CD45RO Antibody | IL-A116

//www.bio-rad-antibodies.com/cow-bovine-cd45ro-antibody-il-a116-mca2434ga.html?RenderPageSpecific=1
CD45RO Antibody | IL-A116 gallery image 1

Staining of bovine peripheral blood lymphocytes with Mouse anti Bovine CD45RO (MCA2434GA) followed by Goat anti Mouse IgG:FITC (STAR117F)

Enlarge
CD45RO Antibody | IL-A116 gallery image 2

Staining of bovine peripheral blood lymphocytes with Mouse anti Bovine CD45RO:RPE (MCA2434PE)

Enlarge
  • CD45RO Antibody | IL-A116 thumbnail image 1
  • CD45RO Antibody | IL-A116 thumbnail image 2
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
    IL-A116
  • Isotype
    IgG3
2 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA2434GAF, IPdatasheet pdfdatasheet pdf0.1 mg
    MCA2434GA
    MCA2434PEFdatasheet pdfdatasheet pdf100 Tests
    MCA2434PE
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Mouse anti Bovine CD45RO, clone IL-A116, specifically recognises the bovine homologue of the human CD45RO cell surface antigen.

      CD45, also known as Leucocyte Common Antigen or LCA, occurs in a number of isoforms, clone IL-A116 is specific for the low molecular weight isoform termed CD45RO, the isoform associated with expression on memory T-cells. Bovine CD45RO is expressed by monocytes, granulocytes and subsets of thymocytes, CD4+ T cells and CD8+ T cells. Studies utilizing clone IL-A116 have demonstrated that the percentage of CD45RO+ CD8+ T cells increase from approximately 5% in neonatal calves to approximately 35% in adult cattle over the age of 5 years (Hogg et al. 2011). It has been demonstrated that mouse anti bovine CD45RO, clone IL-A116 immunoprecipitates a molecule of 180kDa (Bembridge et al 1995) which is analogus to the molecular weight of human and mouse CD45RO.

      Clone IL-A116 has been demonstrated to recognise the CD45RO cell surface antigen by flow cytometry in both European cattle, Bos taurus, and in Zebu, B.indicus (Bembridge et al 1995).
    • Intended Use
    • Target Species
      Bovine
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      Goatyes
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilised
      Purified IgG - liquid
    • Reconstitution
      Reconstitute with 1.0 ml distilled water
      Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
    • Preservative Stabilisers
      0.09% Sodium Azide (NaN3)
      1% Bovine Serum Albumin
      5% Sucrose
      0.09% Sodium Azide (NaN3)
    • Immunogen
      Bovine peripheral blood monouclear cells.
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 1.0 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
    • Fusion Partners
      Spleen cells from immunised BALB/c mice were fused with cells of the X63.Ag8.653 myeloma cell line.
    • Storage
      Prior to reconstitution store at +4oC.
      Following reconstitution store at +4oC.
      DO NOT FREEZE.
      This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      12 months from date of reconstitution
      18 months from date of despatch
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1/101/50
      Immunoprecipitation

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • Flow Cytometry
      Use 10ul of the suggested working dilution to label 1x106 cells in 100ul
    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use

    Additional CD45RO Antibody Formats

    Formats Clone Applications Sizes available
    CD45RO Antibody : Purified IL-A116 F, IP 0.1 mg
    CD45RO Antibody : RPE IL-A116 F 100 Tests
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              References

              Write your review

              You may also be interested in...