CD4 Antibody | CC30

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CD4 Antibody | CC30 gallery image 1

Published customer image:
Mouse anti Bovine CD4 antibody, clone CC30 used to demonstrate the presence of T helper cells in ovine placenta during gestation by immunohistochemistry on cryostat sections.
Image caption:
Comparison of the immunohistochemical labelling of T inflammatory cells and parasite antigen in the placenta. This panel compares the distribution and frequency of T lymphocytes (CD3, CD4 and CD8 antigens) and parasite antigen immunohistochemically labelled in samples from the three groups. Pictures showing the labelling of T cells (first three rows) were taken at 75× and those of the parasite antigen (last row) were taken at 150×. F: foetal mesenchyme, M: maternal endometrial stalk.

From: Arranz-Solís D, Benavides J, Regidor-Cerrillo J, Horcajo P, Castaño P, Del Carmen Ferreras M, Jiménez-Pelayo L, Collantes-Fernández E, Ferre I, Hemphill A, Pérez V, Ortega-Mora LM.
Systemic and local immune responses in sheep after Neospora caninum experimental infection at early, mid and late gestation.
Vet Res. 2016 Jan 6;47(1):2.

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CD4 Antibody | CC30 gallery image 2

Published customer image:
Mouse anti Bovine CD4 antibody, clone CC30 used for the detection of CD4+ve helper T cells by immunohistochemistry on formalin fixed, paraffin embedded placental sections.
Image caption:
Examples of different immune cell infiltration in placentomes from N. caninum-inoculated animals. (a) Severe infiltration of macrophages in the base of maternal caruncle of a N. caninum inoculated cow culled 14 dpi. Counterstained with haematoxylin. Original magnification 100×. (b) Mild aggregate of T helper cells (CD4+) surrounding a blood vessel in maternal caruncle of a placentome collected from an inoculated cow culled 28 dpi. Counterstained with haematoxylin. Original magnification 200×. (c) Minimal infiltration of cytotoxic T-cells (CD8+) in a necrotic focus in maternal caruncle of a N. caninum challenged cow culled 28 dpi. Counterstained with haematoxylin. Original magnification 200×. (d) Mild aggregate of γδ T-cells in the base of maternal caruncle of an inoculated cow culled 14 dpi. Counterstained with haematoxylin. Original magnification 200×. (e) Rare presence of NK-cells in connective tissue in the maternal caruncle surrounding necrotic and mineralized foetal villi, observed in a placenta of an inoculated cow culled 28 dpi. Counterstained with haematoxylin. Original magnification 200×. (f) Rare presence of CD79αcy+ cells aligned with endometrial epithelial cells in the maternal caruncle and in some foetal villi, observed in the placentome of a N. caninum inoculated cow culled 28 dpi. Original magnification 400×. Counterstained with haematoxylin.

From: Cantón GJ, Katzer F, Benavides-Silván J, Maley SW, Palarea-Albaladejo J, Pang Y, Smith S, Bartley PM, Rocchi M, Innes EA, Chianini F.
Phenotypic characterisation of the cellular immune infiltrate in placentas of cattle following experimental inoculation with Neospora caninum in late gestation.
Vet Res. 2013 Jul 22;44:60.

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  • Mouse anti Bovine CD4
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
    CC30
  • Isotype
    IgG1
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA834GAC, F, IP, P *datasheet pdfdatasheet pdf0.1 mg
    MCA834GA
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Mouse anti Bovine CD4 antibody, clone CC30 recognizes a ~50 kDa transmembrane molecule considered to be the bovine homologue of human CD4. The phenotype, tissue distribution and function of T-cells expressing the bovine CD4 antigen are similar to those in other species. However, expression on macrophages has not yet been detected.

      Mouse anti Bovine CD4, clone CC30 has successfully been used for immunohostochemical localization of CD4 on paraffin embedded material using zinc salt fixation (Cantón et al. 2013). Additionally, clone CC30 has been reported as being suitable for use on formal dichromate (FD5) fixed paraffin embedded tissue with amplification and antigen retrieval techniques (Gutierrez et al. 1999).
    • Intended Use
    • Target Species
      Bovine
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      Bisonyes
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Purified IgG - liquid
    • Reconstitution
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
    • Preservative Stabilisers
      0.09% Sodium Azide (NaN3)
    • Immunogen
      Bovine thymocytes.
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 1.0 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Fusion Partners
      Spleen cells from an immunised mouse were fused with cells of the mouse NS1 myeloma cell line.
    • Storage
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1/251/200
      Immunohistology - Frozen
      Immunoprecipitation
      Immunohistology - Paraffin(1)
      (1)

      Clone CC30 has been reported as being suitable for use on formal dichromate (FD5) fixed paraffin embedded tissue with amplification and antigen retrieval techniques, see Gutierrez et al. for details.

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • Flow Cytometry
      Use 10ul of the suggested working dilution to label 106 cells in 100ul.
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional CD4 Antibody Formats

    Formats Clone Applications Sizes available
    CD4 Antibody : Purified CC30 C, F, IP, P * 0.1 mg
    • Copyright © 2016 Bio-Rad

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              References

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