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CD1w2 Antibody | CC20
Staining of bovine peripheral blood lymphocytes with Mouse anti Bovine CD1w2:RPE (MCA2058PE)
Enlarge- Mouse anti Bovine CD1w2
- Mouse anti Bovine CD1w2:RPE
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Product TypeMonoclonal Antibody
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CloneCC20
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IsotypeIgG2a
2 Formats Available
| Product Code | Applications | Datasheet | MSDS | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|---|
Summary
Secondary Antibodies
Negative Isotype Controls
Useful Reagents
Positive Controls
Histology Controls
More Images
References
Reviews
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- Mouse anti Bovine CD1w2 antibody, clone CC20 recognises the bovine CD1w2 cell surface antigen, a glycoprotein heterodimer of ~12 kDa and ~46 kDa. CD1w2 is expressed by dendritic cells, cortical thymocytes and a minority of medullary thymocytes, with a pattern similar to antibodies of the CD1b cluster in humans.
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Intended Use
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Target SpeciesBovine
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Species Cross-Reactivity
N.B. Antibody reactivity and working conditions may vary between species.Target Species Cross Reactivity Sheep 
Goat Dog Horse Cat -
Product FormPurified IgG - liquidPurified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
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ReconstitutionReconstitute with 1 ml distilled water
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PreparationPurified IgG prepared by affinity chromatography on Protein G from tissue culture supernatantPurified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
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Preservative Stabilisers
0.09% Sodium Azide 0.09% Sodium Azide 1% Bovine Serum Albumin 5% Sucrose -
Purity
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Approx. Protein ConcentrationsIgG concentration 1.0mg/ml
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Reagents In The Kit
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Preparing The Antibody
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Test Principle
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Buffer SolutionPhosphate buffered salinePhosphate buffered saline
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Fusion PartnersSpleen cells from immunised mice were fused with cells of the mouse NS1 myeloma cell line.
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StorageStore at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.Prior to reconstitution store at +4oC.
After reconstitution store at +4oC.
DO NOT FREEZE. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use. -
Shelf Life18 months from date of despatch.12 months from date of reconstitution.
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Acknowledgements
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RegulatoryFor research purposes only
- This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Yes No Min Dilution Max Dilution Flow Cytometry 1/25 1/50 Immunohistology - Frozen Immunoprecipitation Application Name Yes No Min Dilution Max Dilution Flow Cytometry Neat 1/5 - Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Technical Advice
- Technical Advice
- Recommended Protocol
- Recommended Protocol
- Flow CytometryUse 10ul of the suggested working dilution to label 106 cells in 100ul.
- ELISA
- ELISA
- Immunohistology
- Immunohistology
- Histology Positive Control Tissue
- Histology Positive Control Tissue
- Immunofluorescence
- Immunofluorescence
- Western Blotting
- Western Blotting
- Instructions For Use
- Instructions For Use
Additional CD1w2 Antibody Formats
| Formats | Clone | Applications | Sizes available |
|---|---|---|---|
| CD1w2 Antibody : RPE | CC20 | F | 100 Tests |
| CD1w2 Antibody : Purified | CC20 | C, F, IP | 0.25 mg |
- Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)
Recommended Secondary Antibody
Recommended Negative Isotype Control
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Mouse IgG2a Negative Control | MCA929 | 100 Tests | F | MCA929 |
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Mouse IgG2a Negative Control:RPE | MCA929PE | 100 Tests | F | MCA929PE |
Useful Reagents
Recommended Positive Controls
Histology Controls
Application Name Reference Images Flow Cytometry
References
1. Siedek, E. et al. (1997) Isolation and characterisation of equine dendritic cells.Vet Immunol Immunopathol. 60 (1-2): 15-31.
2. Hein, W.R. et al. (1991) Summary of workshop findings for leukocyte antigens of sheep.Vet Immunol Immunopathol. 27 (1-3): 28-30.
3. Romero-Palomo, F. et al. (2015) Immunopathologic Changes in the Thymus of Calves Pre-infected with BVDV and Challenged with BHV-1.Transbound Emerg Dis. Aug 25. [Epub ahead of print]
4. Howard, C.J. et al. (1993) Comparison of CD1 monoclonal antibodies on bovine cells and tissues.Vet Immunol Immunopathol. 39 (1-3): 77-83.
5. Rhind, S.M. (2001) CD1--the pathology perspective.Vet Pathol. 38 (6): 611-9.
6. Howard, C.J. & Naessens, J. (1993) Summary of workshop findings for cattle (tables 1 and 2).Vet Immunol Immunopathol. 39 (1-3): 25-47.
7. Åkesson, C.P. et al. (2008) Phenotypic characterisation of intestinal dendritic cells in sheep.Dev Comp Immunol. 32: 837-49.
8. Chan, S.S. et al. (2002) Generation and characterization of ovine dendritic cells derived from peripheral blood monocytes.Immunology. 107: 366-72.
9. Shu, D. et al. (2009) Cutaneous cytokine gene expression and cellular responses in lambs infested with the louse, Bovicola ovis, and following intradermal injection of crude louse antigen.Vet Immunol Immunopathol. 129: 82-92.
10. McNeilly, T.N. et al. (2006) Differential expression of cell surface markers by ovine respiratory tract dendritic cells.J Histochem Cytochem. 54: 1021-30.
11. Mérant, C. et al. (2009) Young foal and adult horse monocyte-derived dendritic cells differ by their degree of phenotypic maturity.Vet Immunol Immunopathol. 131: 1-8.
12. Affolter, V.K. and Moore, P.F. (2002) Localized and disseminated histiocytic sarcoma of dendritic cell origin in dogs.Vet Pathol. 39: 74-83.
13. Moore, P.F. et al. (1996) Canine cutaneous histiocytoma is an epidermotropic Langerhans cell histiocytosis that expresses CD1 and specific beta 2-integrin molecules.Am J Pathol. 148: 1699-708.
14. Bienzle, D. et al. (2003) Immunophenotype and functional properties of feline dendritic cells derived from blood and bone marrow.Vet Immunol Immunopathol. 96: 19-30.
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Copyright © 2017 Bio-Rad Laboratories, Inc. All rights reserved
Copyright © 2017 Bio-Rad Laboratories, Inc. All rights reserved
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