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CD1w2 Antibody | CC20

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CD1w2 Antibody | CC20 gallery image 1

Figure A. FITC conjugated Mouse anti Bovine MHC Class II Monomorphic Antibody (MCA2445F) and RPE conjugated Mouse IgG2a isotype control (MCA929PE). Figure B. FITC conjugated Mouse anti Bovine MHC Class II Monomorphic Antibody (MCA2445F) and RPE conjugated Mouse anti Bovine CD1w2 Antibody (MCA2058PE). All experiments performed on bovine thymocytes in the presence of 10% bovine serum and gated on live, single cell thymocytes. Data acquired on the ZE5™ Cell Analyzer.

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CD1w2 Antibody | CC20 gallery image 2

Figure A. FITC conjugated Mouse anti Bovine CD4 Antibody (MCA1653F) and Mouse IgG2a isotype control (MCA929) conjugated with Rabbit F(ab')2 anti Mouse IgG:RPE Antibody (STAR12A). Figure B. FITC conjugated Mouse anti Bovine CD4 Antibody (MCA1653F) and Mouse anti Bovine CD1w2 Antibody (MCA2058GA) conjugated with Rabbit F(ab')2 anti Mouse IgG:RPE Antibody (STAR12A). All experiments performed on bovine thymocytes in the presence of 10% bovine serum and gated on live, single cell thymocytes. Data acquired on the ZE5™ Cell Analyzer.

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CD1w2 Antibody | CC20 gallery image 3

Figure A. FITC conjugated Mouse anti Bovine CD4 Antibody (MCA1653F) and RPE conjugated Mouse IgG2a isotype control (MCA929PE). Figure B. FITC conjugated Mouse anti Bovine CD4 Antibody (MCA1653F) and RPE conjugated Mouse anti Bovine CD1w2 Antibody (MCA2058PE). All experiments performed on bovine thymocytes in the presence of 10% bovine serum and gated on live, single cell thymocytes. Data acquired on the ZE5™ Cell Analyzer.

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CD1w2 Antibody | CC20 gallery image 4

Figure A. FITC conjugated Mouse anti Bovine MHC Class II Monomorphic Antibody (MCA2445F) and Mouse IgG2a isotype control (MCA929) conjugated with Rabbit F(ab')2 anti Mouse IgG:RPE Antibody (STAR12A). Figure B. FITC conjugated Mouse anti Bovine MHC Class II Monomorphic Antibody (MCA2445F) and Mouse anti Bovine CD1w2 Antibody (MCA2058GA) conjugated with Rabbit F(ab')2 anti Mouse IgG:RPE Antibody (STAR12A). All experiments performed on bovine thymocytes in the presence of 10% bovine serum and gated on live, single cell thymocytes. Data acquired on the ZE5™ Cell Analyzer.

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CD1w2 Antibody | CC20 gallery image 5

Detection of Mouse anti Bovine CD1w2 (MCA2058GA) in an immunohistochemical analysis on acetone-fixed frozen bovine thymus using the Histar detection system (STAR3000), original magnification x60.

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  • Mouse anti Bovine CD1w2
  • Mouse anti Bovine CD1w2:RPE

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  • Product Type
    Monoclonal Antibody
  • Clone
    CC20
  • Isotype
    IgG2a
2 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA2058GC, F, IPdatasheet pdfdatasheet pdf0.25 mg
    MCA2058G
    MCA2058PEFdatasheet pdfdatasheet pdf100 Tests
    MCA2058PE
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Mouse anti Bovine CD1w2 antibody, clone CC20 recognises the bovine CD1w2 cell surface antigen, a glycoprotein heterodimer of ~12 kDa and ~46 kDa. CD1w2 is expressed by dendritic cells, cortical thymocytes and a minority of medullary thymocytes, with a pattern similar to antibodies of the CD1b cluster in humans.
    • Intended Use
    • Target Species
      Bovine
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      Sheepyes
      Goatyes
      Dogyes
      Horseyes
      Catyes
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Purified IgG - liquid
      Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
    • Reconstitution
      Reconstitute with 1 ml distilled water
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
    • Preservative Stabilisers
      0.09%Sodium Azide
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      5%Sucrose
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 1.0mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
    • Fusion Partners
      Spleen cells from immunised mice were fused with cells of the mouse NS1 myeloma cell line.
    • Storage
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Prior to reconstitution store at +4oC.
      After reconstitution store at +4oC.
      DO NOT FREEZE. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
      12 months from date of reconstitution.
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1/251/50
      Immunohistology - Frozen1/1000
      Immunoprecipitation
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat1/5

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • Flow Cytometry
      Use 10ul of the suggested working dilution to label 106 cells in 100ul.
    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use

    Additional CD1w2 Antibody Formats

    Formats Clone Applications Sizes available
    CD1w2 Antibody : RPE CC20 F 100 Tests
    CD1w2 Antibody : Purified CC20 C, F, IP 0.25 mg
    • Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)STAR117A0.5 mgE, WB
      STAR117A
      Goat anti Mouse IgG/A/M:Alk. Phos.STAR87A1 mgC, E, WB
      STAR87A
      Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)STAR117D488GA0.1 mgF, IF
      STAR117D488GA
      Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed)STAR117D549GA0.1 mgF, IF, WB
      STAR117D549GA
      Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed)STAR117D649GA0.1 mgF, IF
      STAR117D649GA
      Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)STAR117D680GA0.1 mgF, WB
      STAR117D680GA
      Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)STAR117D800GA0.1 mgF, IF, WB
      STAR117D800GA
      Rabbit F(ab')2 anti Mouse IgG:Dylight®800STAR8D800GA0.1 mgF, IF, WB
      STAR8D800GA
      Human anti Mouse IgG2a:FITCHCA037F0.1 mgF
      HCA037F
      Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)STAR117F0.5 mgF
      STAR117F
      Goat anti Mouse IgG:FITC (Rat Adsorbed)STAR700.5 mgF
      STAR70
      Goat anti Mouse IgG (Fc):FITCSTAR120F1 mgC, F
      STAR120F
      Rabbit F(ab')2 anti Mouse IgG:FITCSTAR9B1 mgF
      STAR9B
      Human anti Mouse IgG2a:HRPHCA037P0.1 mgE
      HCA037P
      Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)STAR117P0.5 mgE, WB
      STAR117P
      Goat anti Mouse IgG:HRP (Rat Adsorbed)STAR770.5 mgC, E, P
      STAR77
      Goat anti Mouse IgG (Fc):HRPSTAR120P1 mgE, WB
      STAR120P
      Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)STAR13B1 mgC, E, P, RE, WB
      STAR13B
      Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)STAR87P1 mgE
      STAR87P
      Rabbit F(ab')2 anti Mouse IgG:RPESTAR12A1 mlF
      STAR12A
      Goat anti Mouse IgG:RPE (Rat Adsorbed)STAR761 mlF
      STAR76

      Recommended Negative Isotype Control

        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Mouse IgG2a Negative ControlMCA929100 TestsF
        MCA929
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Mouse IgG2a Negative Control:RPEMCA929PE100 TestsF
        MCA929PE

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              • Application NameReference Images
                Flow Cytometry

              References

              1. Siedek, E. et al. (1997) Isolation and characterisation of equine dendritic cells.
              Vet Immunol Immunopathol. 60 (1-2): 15-31.
              2. Hein, W.R. et al. (1991) Summary of workshop findings for leukocyte antigens of sheep.
              Vet Immunol Immunopathol. 27 (1-3): 28-30.
              3. Romero-Palomo, F. et al. (2015) Immunopathologic Changes in the Thymus of Calves Pre-infected with BVDV and Challenged with BHV-1.
              Transbound Emerg Dis. Aug 25. [Epub ahead of print]
              4. Howard, C.J. et al. (1993) Comparison of CD1 monoclonal antibodies on bovine cells and tissues.
              Vet Immunol Immunopathol. 39 (1-3): 77-83.
              5. Rhind, S.M. (2001) CD1--the pathology perspective.
              Vet Pathol. 38 (6): 611-9.
              6. Howard, C.J. & Naessens, J. (1993) Summary of workshop findings for cattle (tables 1 and 2).
              Vet Immunol Immunopathol. 39 (1-3): 25-47.
              7. Åkesson, C.P. et al. (2008) Phenotypic characterisation of intestinal dendritic cells in sheep.
              Dev Comp Immunol. 32: 837-49.
              8. Chan, S.S. et al. (2002) Generation and characterization of ovine dendritic cells derived from peripheral blood monocytes.
              Immunology. 107: 366-72.
              9. Shu, D. et al. (2009) Cutaneous cytokine gene expression and cellular responses in lambs infested with the louse, Bovicola ovis, and following intradermal injection of crude louse antigen.
              Vet Immunol Immunopathol. 129: 82-92.
              10. McNeilly, T.N. et al. (2006) Differential expression of cell surface markers by ovine respiratory tract dendritic cells.
              J Histochem Cytochem. 54: 1021-30.
              11. Mérant, C. et al. (2009) Young foal and adult horse monocyte-derived dendritic cells differ by their degree of phenotypic maturity.
              Vet Immunol Immunopathol. 131: 1-8.
              12. Affolter, V.K. and Moore, P.F. (2002) Localized and disseminated histiocytic sarcoma of dendritic cell origin in dogs.
              Vet Pathol. 39: 74-83.
              13. Moore, P.F. et al. (1996) Canine cutaneous histiocytoma is an epidermotropic Langerhans cell histiocytosis that expresses CD1 and specific beta 2-integrin molecules.
              Am J Pathol. 148: 1699-708.
              14. Bienzle, D. et al. (2003) Immunophenotype and functional properties of feline dendritic cells derived from blood and bone marrow.
              Vet Immunol Immunopathol. 96: 19-30.
              15. Romero-palomo, F. et al. (2013) Immunohistochemical detection of dendritic cell markers in cattle.
              Vet Pathol. 50 (6): 1099-108.

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