CD172a Antibody | CC149

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CD172a Antibody | CC149 gallery image 1

Staining of bovine peripheral blood lymphocytes with Mouse anti Bovine CD172a (MCA2041GA) followed by Goat anti Mouse IgG:FITC (STAR70)

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CD172a Antibody | CC149 gallery image 2

Staining of bovine peripheral blood monocytes with Mouse anti Bovine CD172a:RPE-Cy5 (MCA2041C)

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Published customer image:
Mouse anti Bovine CD172α antibody, clone CC149 used for the detection of SIRP1α expressing cells in bovine PBMCs by flow cytometry.
Image caption:
Expansion of CD172a+ cells in response to ESAT-6/CFP-10 stimulation. Isolated PBMC were stained with PKH67 and cultured for 6d with media only, rMPB83, a pool of overlapping ESAT-6 and CFP-10 peptides, or rESAT-6:CFP-10. Data are depicted as: (A) dot plots (CD172a-PE (y-axis) versus PKH67 (x-axis, green fluorescence), (B) histograms generated by Modfit Proliferation Wizard analysis of PKH67 staining intensity (gated on CD172a-PE+ cells within the live gate) and (C) mean (±SEM) percent CD172a+ cells within PBMC cultures (stimulation indicated in the lower margin) from non-infected (open bars, n = 9, includes non- (n = 3), BCG- (n = 3), and ?RD1- (n = 3) vaccinates) or M. bovis-infected (closed bars, n = 20) cattle. Responses did not differ between controls, BCG- and ?RD1- vaccinates; thus, these groups were combined. Gate R2 in panel A highlights the CD172a+, PKH67lo proliferative fraction. For panels A and B, data from a single M. bovis-infected animal are provided that are indicative of a representative response.

From: Waters WR, Palmer MV, Nonnecke BJ, Thacker TC, Estes DM, et al. (2009) Signal Regulatory Protein a (SIRPa)+ Cells in the Adaptive Response to ESAT-6/CFP-10 Protein of Tuberculous Mycobacteria.
PLoS ONE 4(7): e6414.

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CD172a Antibody | CC149 gallery image 4

Published customer image:
Mouse anti Bovine CD172α antibody, clone CC149 used for the detection of SIRP1α expressing cells in cultured bovine PBMCs by immunofluorescence.
Image caption:
CD172a cells bind rESAT-6:CFP-10 and M. bovis BCG. CD172a+ cells (PE-labeled) were isolated by high-speed cell sorting (>98% purity) from rESAT-6:CFP-10-stimulated PBMC 6d cultures; incubated with rESAT-6:CFP-10-FITC or BCG-FITC for 2–96 hrs; and evaluated by fluorescence microscopy. (A) rESAT-6:CFP-10-FITC bound to the surface of CD172a+ cells in a focal pattern (24 hr cultures shown). Labeling patterns were similar at 2, 24 (shown in Fig. 3A) and 96 hrs after addition of rESAT-6:CFP-10 (FITC) to cells, except for increased polarization of staining at 96 hrs. (B) Over the 96 hr culture period, PE-staining (red) used for CD172a+ cell sorting persisted (24 hr cultures shown) with a polar distribution, possibly due to capping of antibody bound to CD172a. However, rESAT-6:CFP-10-labeling (green) did not overlap with CD172a-PE labeling (red). (C) M. bovis BCG was detected in association with CD172a+ cells at 2 (not shown) and 24 hrs (green, intact bacteria associated with the cell surface, panel C) after addition of the live bacteria to the CD172a+ cell culture and by 96 hrs, M. bovis BCG was internalized and mostly degraded (D). White bar = 10 µm.

From: Waters WR, Palmer MV, Nonnecke BJ, Thacker TC, Estes DM, et al. (2009) Signal Regulatory Protein a (SIRPa)+ Cells in the Adaptive Response to ESAT-6/CFP-10 Protein of Tuberculous Mycobacteria.
PLoS ONE 4(7): e6414.

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CD172a Antibody | CC149 gallery image 5

Published customer image:
Mouse anti Bovine CD172a antibody, clone CC149 (MCA2041GA) used for the identification of CD172a expressing cells in bovine blood by flowcytometry.
Image caption:
Adaptation of the reverse-transmigration in vitro model with bovine cells to recapitulate monocyte-derived cells mobilization from blood to the draining lymph node under inflammatory condition. A-D Schematic representation of the in vitro differentiation of blood monocytes into macrophages and Mo-DCs using the reverse-transmigration model. E-I Two-color flow cytometry assay of monocyte-derived cells collected from monocyte/BAEC cultures grown on collagen-coated transwells according to the reverse-transmigration protocol. Non-adherent and low-adherent cells within the top and bottom sections of the transwell were processed for cytometry analysis. Left contour plots show the conditions in the absence of any exogenous stimulus whereas right contour plots show conditions with zymosan. Cells were labeled with anti-SIRP1-α to discriminate monocyte-derived cells from endothelial cells. The expression of MHCII within the SIRP1-α+ population was also determined to discriminate within monocyte-derived cells potential macrophages (MHCII+) and potential Mo-DC (MHCIIhi) (E). The percentages of SIRP1-α+MHCII+, SIRP1-α+MHCIIhi and BAECs in each representative dot plot are indicated (E). F Comparison showing the relative number of SIRP1-α+MHCII+ cells in the bottom of the transwell in conditions with or without zymosan stimulation. G Comparison showing the relative number of SIRP1-α+MHCII+ cells in the top section of the transwell in conditions with or without zymosan stimulation. H Comparison showing the relative number of SIRP1-α+MHCIIhi cells in the top section of the transwell in conditions with or without zymosan stimulation. i Comparison showing the relative expression level of MHCII in SIRP1-α+MHCII+ cells in the top section of the transwell in conditions with or without zymosan stimulation. F-I The value of the control condition was arbitrarily set to 1. Data are presented as the mean?±?SD based on four independent experiments each including at least three transwells per condition. Statistically significant differences between the two groups were determined by the Student's t test, *P?
From: Vachiery N, Puech C, Cavelier P, Rodrigues V, Aprelon R, Lefrançois T, Martinez D, Epardaud M. An in vitro model to assess the immunosuppressive effect of tick saliva on the mobilization of inflammatory monocyte-derived cells.
Vet Res. 2015 Sep 28;46(1):117.

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CD172a Antibody | CC149 gallery image 6

Published customer image:
Mouse anti Bovine CD172a antibody, clone CC149 (MCA2041GA) used for the identification of CD172a expressing cells in bovine blood by flowcytometry.
Image caption:
In vitro characterization of the dual immunosuppressive effect of tick saliva on inflammatory-induced mobilization of monocyte-derived mononuclear phagocytes. A-E Two-color flow cytometry assay of monocyte-derived cells collected from monocyte/BAEC cultures grown on collagen-coated transwells for 48 h according to the reverse-transmigration protocol. Left contour plots show conditions with zymosan alone whereas right contour plots show conditions with zymosan and tick saliva. B Comparison showing the relative number of SIRP1-α+MHCII+ cells in the bottom of the transwell in conditions with zymosan alone or with zymosan and saliva. C Comparison showing the relative number of SIRP1-α+MHCII+ cells in the top section of the transwell in conditions with zymosan alone or with zymosan and saliva. D Comparison showing the relative number of SIRP1-α+MHCIIhi cells in the top section of the transwell in conditions with zymosan alone or with zymosan and saliva. E Comparison showing the relative expression level of MHCII in SIRP1-α+MHCII+ cells in the top section of the transwell in conditions with zymosan alone or with zymosan and saliva. B-E The value for the control condition (zymosan alone) was arbitrarily set to 1. Data are presented as the mean?±?SD based on four independent experiments each including at least three transwells per condition. Statistically significant differences between the two groups were determined by the Student's t test, *P?in vivo. (1) Monocytes from the blood are recruited to the area of the tick bite where a proportion of the cells differentiate into potential macrophages. (2) Potential Mo-DC precursors then migrate from this area into the draining lymphatic vessels.

From: Vachiery N, Puech C, Cavelier P, Rodrigues V, Aprelon R, Lefrançois T, Martinez D, Epardaud M. An in vitro model to assess the immunosuppressive effect of tick saliva on the mobilization of inflammatory monocyte-derived cells.
Vet Res. 2015 Sep 28;46(1):117.

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  • Mouse anti Bovine CD172a:RPE-Cy5
  • Mouse anti Bovine CD172a
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  • Product Type
    Monoclonal Antibody
  • Clone
    CC149
  • Isotype
    IgG2b
2 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA2041GAFdatasheet pdfdatasheet pdf0.1 mg
    MCA2041GA
    MCA2041CFdatasheet pdfdatasheet pdf100 Tests/1ml
    MCA2041C
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Mouse anti Bovine CD172a antibody, clone CC149 recognizes bovine CD172a, also known as MyD-1 antigen and SIRPA. CD172a is a ~55 kDa single pass type 1 membrane protein belonging to the family of signal regulatory proteins (SIRP). CD172a has been identified as the receptor for CD47.

      Bovine CD172a is strongly expressed by splenic macrophages, monocytes and a subset of afferent lymph veiled cells (ALVC) and by dendritic cells in the skin.
    • Intended Use
    • Target Species
      Bovine
    • Product Form
      Purified IgG conjugated to R. Phycoerythrin (RPE) -Cy5 - Lyophilised.
      Purified IgG - liquid
    • Reconstitution
      Reconstitute with 1.0ml distilled water
      Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
    • Preservative Stabilisers
      0.09% Sodium Azide (NaN3)
      1% Bovine Serum Albumin
      5% Sucrose
      0.09% Sodium Azide (NaN3)
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 1.0 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
    • Storage
      Store at +4oC. DO NOT FREEZE.
      This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      12 months from date of reconstitution.
      18 months from date of despatch.
    • GO Terms
      SH3 domain binding
      integral to membrane
    • UniProt
    • Entrez Gene
    • Acknowledgements
      Cy® and CyDye® are registered trademarks of GE Healthcare
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1/1001/200

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • Flow Cytometry
      Use 10ul of the suggested working dilution to label 106 cells in 100ul.
    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use

    Additional CD172a Antibody Formats

    Formats Clone Applications Sizes available
    CD172a Antibody : Purified CC149 F 0.1 mg
    CD172a Antibody : RPE-Cy5 CC149 F 100 Tests/1ml
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)STAR117A0.5 mgE, WB
      STAR117A
      Goat anti Mouse IgG/A/M:Alk. Phos.STAR87A1 mgC, E, WB
      STAR87A
      Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)STAR117D488GA0.1 mgF, IF
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      Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed)STAR117D549GA0.1 mgF, IF
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      Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed)STAR117D649GA0.1 mgF, IF
      STAR117D649GA
      Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)STAR117D680GA0.1 mgF, WB
      STAR117D680GA
      Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)STAR117D800GA0.1 mgF, IF, WB
      STAR117D800GA
      Rabbit F(ab')2 anti Mouse IgG:Dylight®800STAR8D800GA0.1 mgF, IF, WB
      STAR8D800GA
      Human anti Mouse IgG2b:FITCHCA038F0.1 mgF
      HCA038F
      Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)STAR117F0.5 mgF
      STAR117F
      Goat anti Mouse IgG:FITC (Rat Adsorbed)STAR700.5 mgF
      STAR70
      Goat anti Mouse IgG (Fc):FITCSTAR120F1 mgC, F
      STAR120F
      Rabbit F(ab')2 anti Mouse IgG:FITCSTAR9B1 mgF
      STAR9B
      Human anti Mouse IgG2b:HRPHCA038P0.1 mgE
      HCA038P
      Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)STAR117P0.5 mgE, WB
      STAR117P
      Goat anti Mouse IgG:HRP (Rat Adsorbed)STAR770.5 mgC, E, P
      STAR77
      Goat anti Mouse IgG (Fc):HRPSTAR120P1 mgE, WB
      STAR120P
      Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)STAR13B1 mgC, E, P, RE, WB
      STAR13B
      Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)STAR87P1 mgE
      STAR87P
      Rabbit F(ab')2 anti Mouse IgG:RPESTAR12A1 mlF
      STAR12A
      Goat anti Mouse IgG:RPE (Rat Adsorbed)STAR761 mlF
      STAR76
      Human anti Mouse IgG2b:RPEHCA038PE100 TestsF
      HCA038PE

      Recommended Negative Isotype Control

        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Mouse IgG2b Negative Control:RPE-Cy5MCA691C100 TestsF
        MCA691C
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Mouse IgG2b Negative ControlMCA691100 TestsF
        MCA691

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Mouse anti Bovine CD205:FITCMCA1651F0.1 mgF
          MCA1651F
          Bovine Dendritic Cell Growth KitPBP014KZZ1 mlFN
          PBP014KZZ
          Bovine Dendritic Cell Growth KitPBP015KZZ5 mlFN
          PBP015KZZ
          Mouse anti Bovine CD205MCA1651GA0.1 mgC, F, IP
          MCA1651GA

          Recommended Positive Controls

            Histology Controls

              • Application NameReference Images
                Immunofluorescence

              References

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