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CD268 / BAFF-R Antibody | 2C4

CD268 / BAFF-R Antibody | 2C4 gallery image 1

Mouse anti Chicken CD268 / BAFF-R, clone 2C4.

Paraffin sections of bursal tissue were stained with the mouse anti chicken CD268 / BAFF-R antibody, clone 2C4 (2.5 μg/ml) followed by horse anti mouse Ig secondary antibody conjugated to biotin. Visualization was undertaken using a Vector ABC Kit and peroxidase activity was developed using 3.3´ diamino-benzidine-tetrahydro-chloride (DAB).
Image courtesy of Sonja Härtle, Institute for Animal Physiology, University of Munich

CD268 / BAFF-R Antibody | 2C4 gallery image 2

Mouse anti Chicken CD268 / BAFF-R

Peripheral blood lymphocytes were separated by density centrifugation and first stained with the mouse anti chBAFF-R (clone 2C4) and then with goat anti mouse IgG1 secondary antibody conjugated to FITC, finally blocked with 10% normal mouse serum and followed by staining with the mouse anti chicken Bu-1a/b (clone AV20) conjugated to APC.
Image courtesy of Sonja Härtle, Institute for Animal Physiology, University of Munich

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  • CD268 / BAFF-R Antibody | 2C4 thumbnail image 2
  • Mouse anti Chicken CD268 / BAFF-R
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  • Product Type
    Monoclonal Antibody
  • Clone
  • Isotype
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA6011GAC, F, Pdatasheet pdfdatasheet pdf0.1 mg
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    • Mouse anti Chicken CD268 / BAFF-R, clone 2C4, recognises the chicken homologue of human BAFF-R, known also as CD268 and TNFRSF 13C, a cell surface antigen expressed by avian B-cells.

      Chicken BAFF-R (B cell activating factor receptor) differs from mammalian BAFF-R in that it is produced in B-cells (Reddy et al. 2008) and is not expressed by chicken T-cells. In addition to chicken, clone 2C4 has been shown to cross react with several other commercially important avian species and as such is a specific marker of B-cells for research in domestic galliforms.

      Clone 2C4 is unique in that it can be used in formalin fixed paraffin embbeded tissues and is therefore of particular use on sections from infection studies.
    • Intended Use
    • Target Species
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Purified IgG - liquid
    • Reconstitution
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
    • Preservative Stabilisers
      0.09% Sodium Azide (NaN3)
    • Immunogen
      Chicken BAFF-R transfected HEK293 cells
    • Purity
    • Approx. Protein Concentrations
      IgG concentration1.0 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Storage
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry
      Immunohistology - Frozen
      Immunohistology - Paraffin

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • Flow Cytometry
      Use 10μl of the suggested working dilution to label 1x106 cells in 100ul
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional CD268 / BAFF-R Antibody Formats

    Formats Clone Applications Sizes available
    CD268 / BAFF-R Antibody : Purified 2C4 C, F, P 0.1 mg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)STAR117A0.5 mgE, WB
      Goat anti Mouse IgG/A/M:Alk. Phos.STAR87A1 mgC, E, WB
      Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)STAR117D488GA0.1 mgF, IF
      Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed)STAR117D549GA0.1 mgF, IF
      Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed)STAR117D649GA0.1 mgF, IF
      Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)STAR117D680GA0.1 mgF, WB
      Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)STAR117D800GA0.1 mgF, IF, WB
      Rabbit F(ab')2 anti Mouse IgG:Dylight®800STAR8D800GA0.1 mgF, IF, WB
      Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)STAR117F0.5 mgF
      Goat anti Mouse IgG:FITC (Rat Adsorbed)STAR700.5 mgF
      Goat anti Mouse IgG (Fc):FITCSTAR120F1 mgC, F
      Rabbit F(ab')2 anti Mouse IgG:FITCSTAR9B1 mgF
      Human anti Mouse IgG1:HRPHCA036P0.1 mgE
      Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)STAR117P0.5 mgE, WB
      Goat anti Mouse IgG:HRP (Rat Adsorbed)STAR770.5 mgC, E, P
      Goat anti Mouse IgG (Fc):HRPSTAR120P1 mgE, WB
      Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)STAR13B1 mgC, E, P, RE, WB
      Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)STAR87P1 mgE
      Rabbit F(ab')2 anti Mouse IgG:RPESTAR12A1 mlF
      Goat anti Mouse IgG:RPE (Rat Adsorbed)STAR761 mlF

      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Mouse anti Chicken Bu-1A:FITCMCA2170F0.1 mgF
          Mouse anti Guinea Fowl Bu-1a/bMCA5958GA0.1 mgC, IP, WB
          Mouse anti Chicken Bu-1a/bMCA57640.25 mgC, F, IP
          Mouse anti Chicken Bu-1A:RPEMCA2170PE100 TestsF

          Recommended Positive Controls

            Histology Controls

                • Product Images

                • CD268 / BAFF-R Antibody | 2C4 thumbnail image 1
                • CD268 / BAFF-R Antibody | 2C4 thumbnail image 2

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