"A question of Life or Death" - differentiating between healthy and apoptotic cells
Dead or Alive on the ZE5™ - Using flow cytometry to measure apoptosis
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An increase in programmed cell death, also known as apoptosis can be a good indicator of the health of your cells. An extensive range of antibodies and kits have been designed to easily identify apoptosis, whatever your experiment. A selection of products to determine general levels of apoptosis with quantifiable read outs such as caspase, cathepsin and annexin V levels, as well as common ligands and markers involved in apoptosis, are listed below.
We also offer a more comprehensive list of antibodies against apoptosis cell membrane receptors, CTL mediated apoptosis, and negative regulators of apoptosis.
Figure 1. Jurkat cells were stained with MitoPT JC-1 and viewed through a fluorescence microscope using a broad band path filter. Non-apoptotic cells exhibit red stained mitochondria (2 cells at the top). Apoptotic cells at varying stages of mitochondrial membrane potential appear green (3 cells at the bottom)
We have several kits to determine the degree of apoptosis in a cell population. They are suitable to detect both early apoptosis and late apoptosis. Early apoptosis can be determined by loss of the electrochemical gradient across the mitochondrial membrane and by phosphatidylserine flipping using pSIVA™ kits, whereas late apoptosis can be determined by Annexin V staining to show an increase in phosphatidylserine on the cell surface and DNA fragmentation using BrdU incorporation.
They are available in several formats to allow convenient incorporation into multi-color panels and can be detected by flow cytometry or Imaging.
Figure 2. Western blot analysis of whole cell lysates probed with Bcl-2 antibody followed by detection with HRP conjugated Goat anti-mouse IgG (1/10,000, STAR207P) and visualized on the ChemiDoc MP with 67 second exposure. Arrow points to Bcl-2 (molecular weight 26 kDa
Our range also includes antibodies to detect and measure markers of apoptosis.
Markers of apoptosis include the presence of annexin V on the cell surface, intracellular caspases and cytochrome c, death receptors and anti-apoptotic markers of the Bcl-2 family.
In addition to our apoptosis antibodies and reagents we have antibodies, kits and reagents to detect cell viability, proliferation and autophagy so you can have confidence in the health of your cells.
Magic Red™ and MitoPT™ are trademarks of Immunochemistry Technologies, LLC.
pSIVA™ is a trademark of Novus Biologicals and is protected under patent no. 8,541,549.