Mitochondrial Permeability Transition:MitoPT™ JC-1 Kit

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Mito Permeability Transition Kit - 400 Tests gallery image 1 — Jurkat T cells were stained with MitoPT^TM JC-1 and viewed through a fluorescence microscope using a broad band path filter. Non-apoptotic cells exhibit red stained mitochondria (2 cells at the top). Apoptotic cells at varying stages of mitochondrial membrane potential appear green (3 cells at the bottom)

Mitochondrial Permeability Transition Kit

Product Type: Kits

Product Code	Applications	Pack Size	Your Price	List Price	Quantity
ICT944 Datasheet	F IF MSDS	400 Tests	Log in		Get Quote

Summary

The MitoPT™ JC-1 kit uses a quick and easy staining method to clearly differentiate between non-apoptotic and apoptotic cells through mitochondrial functionality.

Product Details

Reagents in the Kit: MitoPT™ JC-1 Reagent - lyophilized
10X Assay Buffer, 125mL
600uL vial of 50mM Carbonylcyanide m - chlorophenylhydrazone (CCCP) concentrate in DMSO
Test Principle: An early indication of apoptosis involves a collapse in the electrochemical gradient across the mitochondrial membrane. Loss of mitochondrial membrane potential can be detected by a unique fluorescent cationic dye known as JC-1 (5,5’,6,6’-tetrachloro-1,1’,3,3’-tetraethylbenzamidazolocarbocyanin iodide) that has been incorporated into the MitoPT™ JC-1 kit.

The MitoPT™ JC-1 reagent easily penetrates cells and enters the mitochondria. It aggregates in the mitochondria of non-apoptotic cells and fluoresces red, whilst in apoptotic cells it diffuses throughout the cell. Once dispersed, the reagent assumes a monomeric form and fluoresces green. This allows an easy distinction between non-apoptotic red fluorescent cells and apoptotic green fluorescent cells which can be read with a flow cytometer, fluorescence microscope or a fluorescence plate reader using black microtitre plates.

Storage Information

Storage: Store the unopened kit (and each unopened component) at -20°C until the expiration date. Protect the MitoPT™ JC-1 reagent from light at all times.

Once reconstituted, the 100X MitoPT™ JC-1 stock should be stored at -20°C protected from light.
Guarantee: Guaranteed until date of expiry. Please see product label.

More Information

Acknowledgements: MitoPT™> is a trademark of Immunochemistry Technologies, LLC.
Regulatory: For research purposes only

Applications of Mitochondrial Permeability Transition:MitoPT™ JC-1 Kit

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Flow Cytometry
Immunofluorescence

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.