Mitochondrial Permeability Transition:MitoPT™ JC-1 Kit
The MitoPT™ JC-1 kit (ICT944) used to detect mitochondrial depolarization in mouse cells by immunofluorescence.
Image caption:
Polarization of mitochondrial membrane in cancer and normal cells upon UM0112176 treatment. The more yellow appear the mitochondria the higher is the ratio of JC-1 aggregates (red, strong polarization) to monomers of the dye (green, depolarized). Bar = 10μM.
From: Gizak A, Wiśniewski J, Heron P, Mamczur P, Sygusch J, Rakus D.
Targeting a moonlighting function of aldolase induces apoptosis in cancer cells.
Cell Death Dis. 2019 Sep 26;10(10):712.
doi: 10.1038/s41419-019-1968-4.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
The MitoPT™ JC-1 kit uses a quick and easy staining method to clearly differentiate between non-apoptotic and apoptotic cells through mitochondrial functionality.
Product Details
- Reagents in the Kit
- MitoPT JC-1 Reagent, 400 tests
Two bottles 10x Assay Buffer, 125 mL
CCCP (50 mM in DMSO), 600 uL - Test Principle
- An early indication of apoptosis involves a collapse in the electrochemical gradient across the mitochondrial membrane. Loss of mitochondrial membrane potential can be detected by a unique fluorescent cationic dye known as JC-1 (5,5’,6,6’-tetrachloro-1,1’,3,3’-tetraethylbenzamidazolocarbocyanin iodide) that has been incorporated into the MitoPT JC-1 kit.
The MitoPT JC-1 reagent easily penetrates cells and enters the mitochondria. It aggregates in the mitochondria of non-apoptotic cells and fluoresces red, whilst in apoptotic cells it diffuses throughout the cell. Once dispersed, the reagent assumes a monomeric form and fluoresces green. This allows an easy distinction between non-apoptotic red fluorescent cells and apoptotic green fluorescent cells which can be read with a flow cytometer, fluorescence microscope or a fluorescence plate reader using black microtitre plates.
Storage Information
- Storage
- Store the unopened kit and each unopened component at -20°C until the expiration date. Once opened, some components may be stored at 2-8°C until the expiration date. CCCP should be stored frozen. Once reconstituted with DMSO, dilute and use MitoPT reagent immediately, or store at ≤-20°C for 12 months protected from light and thawed no more than twice.
- Guarantee
- Guaranteed until date of expiry. Please see product label.
More Information
- Acknowledgements
- MitoPT™ is a trademark of Immunochemistry Technologies, LLC.
- Regulatory
- For research purposes only
Applications of Mitochondrial Permeability Transition:MitoPT™ JC-1 Kit
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry | |||
| Immunofluorescence |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Instructions For Use
- Instructions for use can be found at www.bio-rad-antibodies.com/uploads/IFU/ICT944.pdf
Copyright © 2020 Bio-Rad Antibodies (formerly AbD Serotec)
Product Specific References
References for Mitochondrial Permeability Transition:MitoPT™ JC-1 Kit
-
Gizak, A. et al. (2019) Targeting a moonlighting function of aldolase induces apoptosis in cancer cells.
Cell Death Dis. 10 (10): 712. -
Jasek, E. et al. (2012) Effect of histone deacetylase inhibitors trichostatin A and valproic acid on etoposide-induced apoptosis in leukemia cells.
Anticancer Res. 32 (7): 2791-9. -
Xiaojuan, W. et al. (2014) In Vitro Comparative Effect of Three Novel Borate Bioglasses on the Behaviors of Osteoblastic MC3T3-E1 Cells
Journal of Materials Science & Technology. 30 (10): 979-83.
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