Mitochondrial Permeability Transition:Mitopt JC-1 Kit





Filter by Application:
F IF ResetMitochondrial Permeability Transition Kit
- Product Type
- Kits
- Specificity
- Mitochondrial Permeability Transition:Mitopt JC-1 Kit
The MitoPT JC-1 kit uses a quick and easy staining method to clearly differentiate between non-apoptotic and apoptotic cells through mitochondrial functionality. |
- Reagents In The Kit
- MitoPT JC-1 Reagent, 100 tests
10x Assay Buffer, 60 mL
CCCP (50 mM in DMSO), 125 ul. - Test Principle
- An early indication of apoptosis involves a collapse in the electrochemical gradient across the mitochondrial membrane. Loss of mitochondrial membrane potential can be detected by a unique fluorescent cationic dye known as JC-1 (5,5’,6,6’-tetrachloro-1,1’,3,3’-tetraethylbenzamidazolocarbocyanin iodide) that has been incorporated into the MitoPT JC-1 kit.
The MitoPT JC-1 reagent easily penetrates cells and enters the mitochondria. It aggregates in the mitochondria of non-apoptotic cells and fluoresces red, whilst in apoptotic cells it diffuses throughout the cell. Once dispersed, the reagent assumes a monomeric form and fluoresces green. This allows an easy distinction between non-apoptotic red fluorescent cells and apoptotic green fluorescent cells which can be read with a flow cytometer, fluorescence microscope or a fluorescence plate reader using black microtitre plates. - Regulatory
- For research purposes only.
- Guarantee
- Guaranteed until date of expiry. Please see product label.
- Acknowledgements
- MitoPT is a trademark of Immunochemistry Technologies, LLC.
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
Flow Cytometry | ![]() |
||
Immunofluorescence | ![]() |
- Instructions For Use
-
ICT943
Instructions for use can be found at www.bio-rad-antibodies.com/uploads/IFU/ICT943.pdf
How to Use the Spectraviewer
Watch the Tool Tutorial Video ▸- Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
- Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
- Select the lasers and filters you wish to include
- Select combined or multi-laser view to visualize the spectra
Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
---|---|---|---|---|---|---|---|
Mitochondrial Permeability Transition Kit | ICT944 | F IF | 400 Tests | Log in | |||
List Price | Your Price | ||||||
Log in | |||||||
Description | Mitochondrial Permeability Transition Kit |
References for Mitochondrial Permeability Transition:Mitopt JC-1 Kit
-
Jasek, E. et al. (2012) Effect of histone deacetylase inhibitors trichostatin A and valproic acid on etoposide-induced apoptosis in leukemia cells.
Anticancer Res. 32 (7): 2791-9. -
Wei, X. et al. (2014) In Vitro Comparative Effect of Three Novel Borate Bioglasses on the Behaviors of Osteoblastic MC3T3-E1 Cells
J Mat Sci Technol. 30 (10): 979-83. -
Gizak, A. et al. (2019) Targeting a moonlighting function of aldolase induces apoptosis in cancer cells.
Cell Death Dis. 10 (10): 712. -
Gizak, A. et al. (2021) A novel remitting leukodystrophy associated with a variant in FBP2.
Brain Commun. 3 (2): fcab036. -
Lasota, M. et al. (2022) Property of a new Prussian blue-bounded iron complex able to peroxidate non-saturated fatty acids with a tendency to create conditions that may encourage ferroptosis.
J Physiol Pharmacol. 73 (5)Mar 16 [Epub ahead of print].
Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
Always be the first to know.
When we launch new products and resources to help you achieve more in the lab.
Yes, sign me up