The Magic Red™ Cathepsin B kit uses a quick and easy method to analyze intracellular Cathepsin B protease activity in whole living cells.
- Reagents in the Kit
- Magic Red™ MR-(RR)2 Reagent - lyophilized
Hoechst 33342 Stain, 1 mL
Acridine orange (AO), 0.5 mL
- Test Principle
- Cathepsin Magic Red™ kits measure cathepsin B protease activity by detecting active cathepsins in whole, living cells. These kits do not work by using antibodies or as an ELISA. Instead, their methodology is based on a cell-permeable and non-cytotoxic reagent which is cleaved in the presence of cathepsins to produce a fluorescent product.
The Magic Red™ reagent contains a cathepsin B target sequence peptide (RR)2 linked to a red (Cresyl Violet) fluorescent probe.
The Cathepsin Magic Red™ kits are suitable for cells in suspension and adherent cells from many species including mammalian, insect and yeast. Different cell types, e.g. Jurkat, HL-60, THP-1, fibroblasts, UMUC-3, MCF-7 and U937 cells have also been successfully studied with these kits.
This kit can be used in fluorescence microscopy or with fluorescence plate readers using black microtitre plates.
- Store the unopened kit (and each unopened component) at +4oC until the expiration date.
Protect the MR-(RR)2 reagent from light at all times.
Once reconstituted, the 260X MR-(RR)2 stock should be stored at -20°C protected from light.
- Guaranteed until date of expiry. Please see product label.
- Magic Red™ is a trademark of Immunochemistry Technologies, LLC.
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of Cathepsin B
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Instructions For Use
- Instructions for use can be found at www.bio-rad-antibodies.com/uploads/IFU/ICT937.pdf
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Useful Reagents Available