Leucoperm™

Leucoperm™ makes intracellular antigen analysis using flow cytometry easy. The kit comes with a ‘Reagent A’ that fixes cells in suspension, and a ‘Reagent B’ that subsequently permeabilizes them.

• Results within the hour
• Negligible background staining
• Optimal staining patterns
• Excellent scatter characteristics

With Leucoperm™, antibodies can access intracellular structures leaving the morphological scatter characteristics of the cells intact. Leucoperm™ is superb for detecting many intracellular antigens including:

• Cytokines e.g. IL-2 & IFN
• TdT, MPO & CD3 involved in leukemia & lymphoma
• Platelets: P-selectin, CD63 & Thrombospondin
• Nuclear Ki-67 & PCNA expression in proliferating cells

The Ideal Fixation and Permeabilization Kits

The specific formulation of Leucoperm™ reduces background staining and allows the simultaneous addition of permeabilization medium and fluorochrome-labeled antibodies.

Most commercially available monoclonal antibody conjugates can be used with Leucoperm™ reagents.