Leucoperm™ makes intracellular antigen analysis using flow cytometry easy. The kit comes with a ‘Reagent A’ that fixes cells in suspension, and a ‘Reagent B’ that subsequently permeabilizes them.
With Leucoperm™, antibodies can access intracellular structures leaving the morphological scatter characteristics of the cells intact. Leucoperm™ is superb for detecting many intracellular antigens including:
The specific formulation of Leucoperm™ reduces background staining and allows the simultaneous addition of permeabilization medium and fluorochrome-labeled antibodies.
Most commercially available monoclonal antibody conjugates can be used with Leucoperm™ reagents.