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Leucoperm™
Leucoperm™ makes intracellular antigen analysis using flow cytometry easy. The kit comes with a ‘Reagent A’ that fixes cells in suspension, and a ‘Reagent B’ that subsequently permeabilizes them.
- Results within the hour
- Negligible background staining
- Optimal staining patterns
- Excellent scatter characteristics
With Leucoperm™, antibodies can access intracellular structures leaving the morphological scatter characteristics of the cells intact. Leucoperm™ is superb for detecting many intracellular antigens including:
- Cytokines e.g. IL-2 & IFN
- TdT, MPO & CD3 involved in leukemia & lymphoma
- Platelets: P-selectin, CD63 & Thrombospondin
- Nuclear Ki-67 & PCNA expression in proliferating cells
The Ideal Fixation and Permeabilization Kits
The specific formulation of Leucoperm™ reduces background staining and allows the simultaneous addition of permeabilization medium and fluorochrome-labeled antibodies.
Most commercially available monoclonal antibody conjugates can be used with Leucoperm™ reagents.