Listed here are alamarBlue® articles and educational resource for anyone interested in learning more about the detailed methods, uses and advantages of alamarBlue®.
Comparisons with alternative cell proliferation assays such as MTT, XTT and [3H]thymidine incorporation assays have been highlighted.
Articles have also been grouped by methods to make it easier to see how alamarBlue® is used in a variety of cell culture applications.
It has been shown by Hamid et al. in 2004, that alamarBlue® is more sensitive than 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide (MTT) for most of the drug compounds they tested in HepG2 cell viability assays.
A study on the use of alamarBlue® with peripheral blood mononuclear cells (PBMC) by De Fries et al. 1995 showed that the alamarBlue® assay reliably detects human PBMC, and the results were highly reproducible. Ahmed et al. in 1994 describe the use of alamarBlue® to monitor and determine the proliferation of murine lymphocytes, lymphoid tumor and hybridoma cells. Cell proliferation can be determined by color change using an ELISA plate reader after alamarBlue® is added during the initial phase of cell culture. It was determined that alamarBlue® gave comparable results to the [3H]thymidine incorporation assay.
The summary of alamarBlue® advantages over the [3H]thymidine incorporation assay include:
alamarBlue® is manufactured for Bio-Rad by Trek Diagnostic System. U.S. patent 5,501,959.
Payment methods we support: