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alamarBlue - Cell Proliferation and Viability Reagent
Listed here are alamarBlue articles and educational resource for anyone interested in learning more about the detailed methods, uses and advantages of alamarBlue.
Comparisons with alternative cell proliferation assays such as MTT, XTT and [3H]thymidine incorporation assays have been highlighted.
Articles have also been grouped by methods to make it easier to see how alamarBlue is used in a variety of cell culture applications.
It has been shown by Hamid et al. in 2004, that alamarBlue is more sensitive than 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide (MTT) for most of the drug compounds they tested in HepG2 cell viability assays.
A study on the use of alamarBlue with peripheral blood mononuclear cells (PBMC) by De Fries et al. 1995 showed that the alamarBlue assay reliably detects human PBMC, and the results were highly reproducible. Ahmed et al. in 1994 describe the use of alamarBlue to monitor and determine the proliferation of murine lymphocytes, lymphoid tumor and hybridoma cells. Cell proliferation can be determined by color change using an ELISA plate reader after alamarBlue is added during the initial phase of cell culture. It was determined that alamarBlue gave comparable results to the [3H]thymidine incorporation assay.
The summary of alamarBlue advantages over the [3H]thymidine incorporation assay include: