This reagent is offered in two sizes:
25 ml = Enough for 2,500 wells
100 ml = Enough for 10,000 wells
Note: Calculations assume 100 µl final volume per well (96-well plate).
alamarBlue can be used in a wide range of scientific research areas and applications including experiments involving cell proliferation, cell viability, bioassays for relative cytotoxicity, cytokine assays, cell metabolism studies, drug susceptibility, and toxicology studies.
Just add the ready-to-use alamarBlue solution to the cells, incubate for at least 1-4 hours at 37°C between pH 6.8 and pH 7.4. Read the fluorescence or absorbance to measure cell respiration as an indicator for proliferation and cytotoxicity.
alamarBlue reduction is regularly measured using absorbance, which gives good levels of accuracy for most experiments, and is particularly easy to use. In general more sensitive readings can be obtained using fluorescence, especially when attempting to measure very small changes in reduction. This is principally because there is little interference from unreduced alamarBlue on the spectra of reduced alamarBlue when measured by fluorescence. However, when measuring by absorption there can be considerable overlap of the oxidized and reduced forms of alamarBlue.