SpyCatcher antibody | AbD41909kg

anti SpyCatcher

Product Type
Monoclonal Antibody
Clone
AbD41909kg
Isotype
IgG
Specificity
SpyCatcher

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Anti-SpyCatcher Antibody, clone AbD41909kg is a chimeric antibody comprising a human Fab portion and a rabbit Fc domain, which specifically binds to SpyCatcher protein with high affinity. SpyCatcher is used for protein ligation in combination with the SpyTag (Zakeri et al. 2012). SpyCatcher is derived from the second immunoglobulin-like collagen adhesin domain (CnaB2) from the fibronectin binding protein (FbaB) of Streptococcus pyogenes.

Anti-SpyCatcher Antibody binds to the original SpyCatcher as well as the optimized derivatives SpyCatcher2 (Keeble et al. 2017) and SpyCatcher3 (Keeble et al. 2019). It recognizes the SpyCatcher variants in uncoupled form as well as when coupled to the SpyTag peptide or to fusion proteins with C-terminally fused SpyTag. N-terminal fusions were not tested.

View a summary of SpyCatcher products

Target Species
Synthetic Peptide
Product Form
Recombinant chimeric human/rabbit IgG antibody (lambda light chain) selected from the HuCAL® phage display library and expressed in a human cell line. In this antibody, the VH-CH1 regions and light chains are human while the Fc region is derived from rabbit IgG. This antibody is supplied as a liquid.
Preparation
Purified IgG prepared by affinity chromatography on Protein A
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.01% Thiomersal
Immunogen
SpyCatcher
Approx. Protein Concentrations
Antibody concentration 1.0 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch
Acknowledgements
This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See bio-rad.com/en-us/trademarks for details.

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/10,000 1/20,000
Flow Cytometry 1/100 1/1,000
Western Blotting 1/10,000 1/20,000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Technical Advice
For further information about recombinant antibodies incorporating SpyTag technology visit SpyTag and SpyCatcher Products
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul

Description Product Code Applications Pack Size List Price Your Price Quantity
Goat anti Rabbit IgG (H/L):HRP STAR124P C E WB 1 mg loader
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Description Goat anti Rabbit IgG (H/L):HRP

Description Product Code Applications Pack Size List Price Your Price Quantity
BiSpyCatcher2 TZC002 IY 1 mg loader
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Description BiSpyCatcher2
BiSpyCatcher2-CYS TZC002CYS IY 1 mg loader
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Description BiSpyCatcher2-CYS
BiSpyCatcher2-CYS3 TZC002CYS3 IY 1 mg loader
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Description BiSpyCatcher2-CYS3
Human IgG1-FcSpyCatcher3 TZC009 IY 1 mg loader
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Description Human IgG1-FcSpyCatcher3
Human IgG2-FcSpyCatcher3 TZC016 IY 0.5 mg loader
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Description Human IgG2-FcSpyCatcher3
Human IgG4-FcSpyCatcher3 TZC018 IY 0.5 mg loader
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Description Human IgG4-FcSpyCatcher3
Human IgA-FcSpyCatcher3 TZC020 IY 0.5 mg loader
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Description Human IgA-FcSpyCatcher3
Hispec Assay Diluent BUF049A E IY 50 ml
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Description Hispec Assay Diluent
SpyCatcher2 TZC001 IY 1 mg loader
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Description SpyCatcher2
SpyCatcher2-CYS TZC001CYS IY 1 mg loader
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Description SpyCatcher2-CYS
SpyCatcher3 TZC025 IY 1 mg loader
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Description SpyCatcher3
Rabbit IgG-FcSpyCatcher3 TZC013 IY 0.5 mg loader
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Description Rabbit IgG-FcSpyCatcher3
Human IgG3-FcSpyCatcher3 TZC017 IY 0.5 mg loader
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Description Human IgG3-FcSpyCatcher3
Human IgG4-Pro-FcSpyCatcher3 TZC019 IY 0.5 mg loader
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Description Human IgG4-Pro-FcSpyCatcher3
SpyCatcher3-CYS TZC025CYS IY 1 mg loader
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Description SpyCatcher3-CYS
Mouse IgG2a-FcSpyCatcher3 TZC012 IY 0.5 mg loader
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Description Mouse IgG2a-FcSpyCatcher3