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SpyTag antibody | AbD51767kg

anti SpyTag

Product Type
Monoclonal Antibody
Clone
AbD51767kg
Isotype
IgG
Specificity
SpyTag

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New HCA406
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Anti-SpyTag Antibody, clone AbD51767kg is a chimeric antibody comprising a human Fab portion and a rabbit Fc domain, which specifically binds to SpyTag protein with high affinity. SpyTag is a 13 amino acid peptide tag deriving from the second immunoglobulin-like collagen adhesin domain (CnaB2) from the fibronectin binding protein (FbaB) of Streptococcus pyogenes and mainly used for protein ligation in combination with the SpyCatcher (Zakeri et al. 2012).

Anti-SpyTag Antibody binds to the original SpyTag as well as the optimized derivatives SpyTag2 (Keeble et al. 2017) and SpyTag3 (Keeble et al. 2019). It recognizes the SpyTag variants in uncoupled form when fused to both N-terminal or C-terminal end of the target protein. The anti-SpyTag Antibody has been shown to not react with any SpyCatcher variants nor with E.coli lysate. Only residual detection of SpyTag ligated to SpyCatchers was observed in ELISA.

Target Species
Synthetic Peptide
Product Form
Recombinant chimeric human/rabbit IgG antibody (lambda light chain) selected from the HuCAL phage display library and expressed in a human cell line. In this antibody, the VH-CH1 regions and light chains are human while the Fc region is derived from rabbit IgG. This antibody is supplied as a liquid.
Preparation
Purified IgG prepared by affinity chromatography on Protein A
Source
HKB-11
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
SpyTag version 1 and 2
Approx. Protein Concentrations
Antibody concentration 1.0 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch
Acknowledgements
This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See bio-rad.com/en-us/trademarks for details.

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/10,000 1/20,000
Western Blotting 1/5,000 1/10,000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Technical Advice
For further information about recombinant antibodies incorporating SpyTag technology visit SpyTag and SpyCatcher Products.

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HCA406

166414 167714

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