HuCAL Fab-F-Spy2-H Negative Control antibody | AbD18705ad

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HuCAL Fab-F-Spy2-H Negative Control Antibody

Product Type
Negative/Isotype Control
Clone
AbD18705ad
Isotype
HuCAL Fab monovalent
Product Code Applications Pack Size List Price Quantity
TZA003
Datasheet
E
SDS
0.1 mg loader

HuCAL Fab-F-Spy2-H negative control, clone AbD18705ad is a recombinant Fab antibody fragment in the format Fab-F-Spy2-H with specificity for green fluorescent protein (GFP). It has no known reactivity with mammalian proteins or other antigens. It has been tested by ELISA for specificity to GFP, but has not been tested in other applications. It is suggested as a negative control reagent when using other HuCAL antibodies of the same Fab format.

It is recommended that this reagent is used at the same concentration as the test reagent.

Product Details

Target Species
Negative Control
Product Form
A monovalent human recombinant Fab (kappa light chain) selected from the HuCAL® phage display library. Expressed in a proprietary E. coli strain. The antibody is tagged with a DYKDDDDK tag, a SpyTag version 2 (VPTIVMVDAYKRYK) and a His6-tag (HHHHHH) at the C-terminus of the antibody heavy chain. This antibody is supplied as liquid.
Preparation
Metal chelate affinity chromatography
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.1% ProClin 950
Immunogen
Green fluorescent protein
Approx. Protein Concentrations
Antibody concentration 0.5 mg/ml

Storage Information

Storage
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Guarantee
12 months from date of despatch

More Information

Acknowledgements
ProClin is a trademark of The Dow Chemical Company (“Dow”) or an affiliated company of Dow
This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See bio-rad.com/en-us/trademarks for details.
His-tag is a registered trademark of EMD Biosciences.
Licensed Use
For in vitro research purposes only, unless otherwise specified in writing by Bio-Rad.
Regulatory
For research purposes only

Applications of HuCAL Fab-F-Spy2-H Negative Control antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Technical Advice
Recommended protocols for coupling a Fab antibody with a SpyTag to a SpyCatcher can be found at SpyTag and SpyCatcher Products, and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual

Useful Reagents Available

Description Product Code Applications Pack Size List Price Quantity
SpyCatcher2 TZC001 IY 1 mg loader
SpyCatcher2-CYS TZC001CYS IY 1 mg loader
BiSpyCatcher2 TZC002 IY 1 mg loader
BiSpyCatcher2-CYS TZC002CYS IY 1 mg loader
BiSpyCatcher2-CYS3 TZC002CYS3 IY 1 mg loader
Human IgG1-FcSpyCatcher3 TZC009 IY 1 mg loader
Mouse IgG2a-FcSpyCatcher3 TZC012 IY 0.5 mg loader
Rabbit IgG-FcSpyCatcher3 TZC013 IY 0.5 mg loader
Human IgG2-FcSpyCatcher3 TZC016 IY 0.5 mg loader
Human IgG3-FcSpyCatcher3 TZC017 IY 0.5 mg loader
Human IgG4-FcSpyCatcher3 TZC018 IY 0.5 mg loader
Human IgG4-Pro-FcSpyCatcher3 TZC019 IY 0.5 mg loader
Human IgA-FcSpyCatcher3 TZC020 IY 0.5 mg loader
SpyCatcher3 TZC025 IY 1 mg loader
SpyCatcher3-CYS TZC025CYS IY 1 mg loader

Fluorescent Spectraviewer

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  • Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
  • Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
  • Select the lasers and filters you wish to include
  • Select combined or multi-laser view to visualize the spectra