MHC Class II H-2I-Ak/s Antibody | OX-6

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Staining of rat spleen cells with Mouse anti H-2I-Ak/s (MCA2687GA)

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Staining of rat spleen cells with Mouse anti H-2I-Ak/s: Alexa Fluor® 647 (MCA2687A647)

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Staining of rat spleen cells with Mouse anti H-2I-Ak/s: Alexa Fluor® 488 (MCA2687A488)

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Staining of rat spleen cells with Mouse anti H-2I-Ak/s: FITC (MCA2687FA)

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Staining of rat spleen cells with Mouse anti H-2I-Ak/s: FITC (MCA2687FB)

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Staining of rat spleen cells with Mouse anti H-2I-Ak/s: RPE (MCA2687PE)

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Mouse anti MHC Class II H-2I-Ak/s antibody, clone OX-6 used for the identification of MHC class II expressing rat retinal microglia by immunofluorescence on cryostat sections.
Image caption:
Activation of microglial cells. Vertical section of retinas from a SD (A-C), untreated P23H (D-F) and tauroursodeoxycholic acid (TUDCA)-treated P23H (G-I) rat stained for Iba1 (green; A, D, G) MHC-II RT 1B (red; B, E, H) or both (C, F, I). Nuclei stained with a nuclear marker (TO-PRO 3, blue). All images were collected from the central area of the retina, close to the optic nerve. GCL, ganglion cell layer; IPL, inner plexiform layer; INL, inner nuclear layer; OPL, outer plexiform layer; ONL, outer nuclear layer. Scale bar: 20 μm.

From: Noailles A, Fernández-Sánchez L, Lax P, Cuenca N. Microglia activation in a model of retinal degeneration and TUDCA neuroprotective effects.
J Neuroinflammation. 2014 Oct 29;11(1):186.

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Mouse anti MHC Class II H-2I-Ak/s antibody, clone OX-6 used for the identification of MHC class II expressing mictoglial cells in rat brain by immunohistochemistry on gormalin fixed cryosections.
Image caption:

Visualization of microglia in midbrain with GSA-I-B4 lectin (A-F) and in motor cortex with OX-42 (G) and OX-6 (H) during symptomatic disease. A, low power view of midbrain reveals enhanced lectin staining in the red nucleus. B, higher magnification shows that enhanced lectin reactivity is confined strictly to red nucleus region (arrows indicate perimeter of red nucleus). C, microglial fusions are interspersed with rubrospinal neurons that appear undamaged. D, lectin-positive microglial fusion (giant cell) within red nucleus. E, oculomotor nucleus reveals normal-appearing motor neurons and lack of microgliosis. F, substantia nigra (pars compacta) shows presence of normal, ramified microglial cells. G, motor cortex shows normal, ramified microglia. H, single, ramified microglial cell positive with OX-6 (arrow) near lateral ventricle. Scale bars: 400 μm (A); 200 μm (E); 100 μm (B,H); 50 μm (C,F,G); 20 μm (D).

From: Fendrick SE, Xue QS, Streit WJ. Formation of multinucleated giant cells and microglial degeneration in rats expressing a mutant Cu/Zn superoxide dismutase gene.
J Neuroinflammation. 2007 Feb 28;4:9.

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Mouse anti MHC Class II H-2I-Ak/s antibody, clone OX-6 used for the identification of MHC class II expressing cells in the rat brain by immunohistochemistry on formalin fixed, paraffin embedded tissue sections.
Image caption:
Representative images of (immuno)histochemical staining of brain tissue (TBI and control) with ED-1, OX-6, GFAP, Perl's, and Fluoro-Jade B ten days after the microdialysis experiment.

From: Folkersma H, Foster Dingley JC, van Berckel BN, Rozemuller A, Boellaard R, Huisman MC, Lammertsma AA, Vandertop WP, Molthoff CF. Increased cerebral (R)-[(11)C]PK11195 uptake and glutamate release in a rat model of traumatic brain injury: a longitudinal pilot study. J Neuroinflammation. 2011 Jun 14;8:67.

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Published customer image:Mouse anti MHC Class II H-2I-Ak/s antibody, clone OX-6 used for the visualization of MHC Class II expressing cells in Rat brain by immunohistochemistry on formalin fixed coronal tissue sections.
Image caption:
Progressive degeneration of the nigral dopaminergic neurons after intrastriatal LPS. (A) Representative TH immunostaining of coronal midbrain sections demonstrates that the numbers of TH-positive neurons and fibers in the substantia nigra pars compacta are gradually reduced by intrastriatal LPS injection. Note that TH-positive neurons in the medial substantia nigra pars compacta and ventral tegmental area are spared; scale bar: 200 μm. (B) Stereological cell counts of the TH-positive neurons in the substantia nigra pars compacta (n = 5–6/group, ** p<0.01, *** p<0.001). (C) The substantia nigra pars compacta is outlined with an orange dashed line (top). High magnification image of Nissl stainings suggest loss of the nigral dopaminergic neurons, at four weeks following LPS injection (bottom); scale bar: 200 μm. (D) Silver staining is hardly seen in the substantia nigra ipsilateral to vehicle treatment. However, abundant silver grain-deposits are observed in the neurons (arrows) and fibers (arrow heads) in the substantia nigra ipsilateral to the intrastriatal LPS injections, indicating there is ongoing neurodegenerative process in the region. Scale bar: 20 μm.

From: Choi D-Y, Liu M, Hunter RL, Cass WA, Pandya JD, et al. (2009) Striatal Neuroinflammation Promotes Parkinsonism in Rats.
PLoS ONE 4(5): e5482.

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Mouse anti MHC Class II H-2I-Ak/s antibody, clone OX-6 used for the evaluation of MHHC class II reactivity in rat brain be immunohistochemistry on coronal sections.
Image caption:
OX-6 IHC staining in the DG. No OX-6 positive cells were identified in P1 D1D2 pups treated with either NS (a) or Dex (b). Brain tumor implant staining as a positive control showed OX-6 positive cells stained brown (c), arrow). Magnification, 400x; scale bar, 50μm.

From: Sze CI, Lin YC, Lin YJ, Hsieh TH, Kuo YM, Lin CH. The role of glucocorticoid receptors in dexamethasone-induced apoptosis of neuroprogenitor cells in the hippocampus of rat pups.
Mediators Inflamm. 2013;2013:628094.

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Mouse anti MHC Class II H-2I-Ak/s antibody, clone OX-6 used for the evaluation of MHC class II expression in the rat brain of transgenic rats by immunohistochemistry on cryostat sections.
Image caption:
Expression of MHC class II molecules is different in brains of transgenic rat models. In brainstem of WKY TG rats (A), activation of microglia is accompanied by widespread MHCII expression, while in SHR TG (B), only sparse MHCII staining was recorded. Stereological quantification shows highly significant differences between the transgenic lines. In WKY TG rats, there are 10 times more microglia that express MHCII than are present in SHR TG rats (C, Student's t-test, *** p < 0.001). Pre-fixed frozen sections. Scale bars: 50 μm.

From: Stozicka Z, Zilka N, Novak P, Kovacech B, Bugos O, Novak M. Genetic background modifies neurodegeneration and neuroinflammation driven by misfolded human tau protein in rat model of tauopathy: implication for immunomodulatory approach to Alzheimer's disease.
J Neuroinflammation. 2010 Oct 12;7:64.

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Mouse anti MHC Class II H-2I-Ak/s antibody, clone OX6 used for the identification of activated microglia in rat brain be immunohistochemistry on formalin fixed cryosections.
Image caption:
Neuroprotection against 6-OHDA toxicity following delayed subchronic intranigral administration of 2R,4R-APDC is associated with reduced numbers of activated microglia in the SNc. Representative photomicrographs (40) of DAB/peroxidase staining for OX-6, a marker of activated microglia in the SNc from each treatment group (scale bar 200μm.). (a) 6-OHDA + vehicle, unlesioned SNc, (b) 6-OHDA + vehicle, lesioned SNc, (c) 6-OHDA + 2R,4R-APDC (10?nmol) unlesioned SNc, (d) 6-OHDA + 2R,4R-APDC (10?nmol) lesioned SNc. Note the increase in intensity of OX-6 staining and the increase in OX-6+ cells displaying morphology of activated microglia in the lesioned SNc of vehicle-treated animal, which appears markedly reduced in animals treated with 2R,4R-APDC.

From: Chan H, Paur H, Vernon AC, Zabarsky V, Datla KP, Croucher MJ, Dexter DT. Neuroprotection and Functional Recovery Associated with Decreased Microglial Activation Following Selective Activation of mGluR2/3 Receptors in a Rodent Model of Parkinson's Disease.
Parkinsons Dis. 2010 May 23;2010. pii: 190450.

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Mouse anti MHC Class II H-2I-Ak/s antibody, clone OX-6 used to identify activated microglia in rat brain be immunohistochemistry on formalin fixed, paraffin embedded tissue sections.
Image caption:
Imatinib reduces infiltration of immune cells and attenuates microglia activation. IHC analysis on paraffin embedded spinal cord tissue cross-sections on day 10 p.i. (A–D; n = 8 rats/experimental group; representative images shown) and 14 p.i. (E–H; n = 5 rats/experimental group; representative images shown). Although showing no sign of CNS inflammation and demyelination, control animals already started recruiting W3/13+ T-cells (B) and ED1+ macrophages (D) to the meninges and in the perivascular space, whereas the imatinib-treated group showed a delay in recruitment of inflammatory cells to the CNS. On day 14 p.i., spinal cords of the imatinib-treated rats exhibiting demyelinated lesions recruited lower amounts of W3/13+ T-cells (G, H) while ED1+ macrophages infiltration was similar to the controls (E, F). Scale bar, 200 μm (A–H). (I–Z) IF performed on spinal cord cross-sections of rats injected with fluorescent tracer (dextran, red) on day 14 p.i. α-Ox-42, ED1, Ox-6, Ox-22, CD45RA and W3/13 antibody staining (all in green) in imatinib- (I, L, O, R, U, X) and PBS-treated rats (J, M, P, S, V, Y). (I–K) Microglia activation was significantly decreased in the imatinib-treated rats, while Ox-42+ cells were detectable around leaky blood vessels (asterix) in the control tissue. (L–N) The amount of macrophages/activated microglia cells was significantly decreased in the spinal cords of the imatinib-treated rats. (O–Q) Significantly lower amounts of MHC class II+ cells were found in the meninges and parenchyma of the imatinib-treated rats vs. PBS controls. (R–Z) Significantly lower amounts of Ox-22+, CD45RA+ and W3/13+ cells were found in the meninges and parenchyma of the imatinib-treated rats vs. PBS controls (R, U, X vs. S, V, Y). Quantifications of Ox-42, ED1, Ox-6, Ox-22, CD45RA and W3/13 expression based on green fluorescent pixel area quantifications from spinal cord cross-sections (K, N, Q, T, W, Z). n = 5 rats/experimental group. Scale bar, 50 μm. Error bars, S.E.M. Statistics were calculated using t-test and P values <0.05 were considered significant (P<0.05 = *, P<0.01 = **, P<0.001 = ***). Imatinib or PBS oral gavage was performed from day 5 p.i until the end of the experiment.

From: Z. Adzemovic M, Zeitelhofer M, Eriksson U, Olsson T, Nilsson I (2013) Imatinib Ameliorates Neuroinflammation in a Rat Model of Multiple Sclerosis by Enhancing Blood-Brain Barrier Integrity and by Modulating the Peripheral Immune Response.
PLoS ONE 8(2): e56586.

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Published customer image:Mouse anti MHC Class II H-2I-Ak/s antibody, clone OX-6 used to identify activated microglia in the rat hippocampus by immunohistochemistry on formalin fixed cryosections.
Image caption:
Microglial activation in response to the “Binge Drinking” regime and β-sultam supplementation. Representative microphotographs of OX-6 positive microglia in the hippocampus of binge-treated rats without (upper panels) and with β-sultam supplementation (lower panels). The micrograph shows colocalisation of OX-6 and iNOS immunoreactivities. Black arrows indicate iNOS, red arrows indicate activated microglia. b: Quantitation of microglial activation is presented. Number of OX-6 immunopositive cells (3-4 rats per group) analysed by two way ANOVA, where the two factors were Binge regime and β-sultam supplementation, followed by the post hoc Fisher's LSD (Protected t-Test) for multiple comparisons. Binge F2,14=66.24, p<0.0001; ethane-β- sultam: F1,14=14.50, p<0.0019; Interaction binge x ethane-β-sultam: F2,14=6.12, p<0.0123; post hoc Bonferroni multiple comparison test: *p<0.05, **p<0.01, ***p<0.001 versus control, #p<0.5, ##<<0.01, ###p<0.001 versus Binge alone.

From: Stefanini C, Colivicchi MA, Della Corte L, Ward RJ, de Witte P, et al. (2014) Ethane-β-Sultam Modifies the Activation of the Innate Immune System Induced by Intermittent Ethanol Administration in Female Adolescent Rats. J Alcohol Drug Depend 2:150.

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Mouse anti MHC Class II H-2I-Ak/s antibody, clone OX-6 used for Minocycline (Mino) treatment significantly diminishes cellular inflammation following ischemia-reperfusion (IR). Mino was delivered as twice-daily intraperitoneal (IP) injections, with two initial dosages of 45 mg/kg prior to ischemia and dosages of 22.5 mg/kg just prior to ischemia and every 12 h for the next 2 d during the reperfusion period. Non-treated (NT) animals received PBS vehicle injections. One eye of each rat was subjected to IR for 45 min or needle puncture only (Sham) and after 48 h of reperfusion retinas were enzymatically dissociated and cells analyzed by flow cytometry. (A) Representative scatter plots of CD11b and CD45 immunostaining intensities of total retinal cells from Sham and IR retinas of rats treated with PBS or Mino. Events are partitioned into quadrant 1 (Q1) containing CD11b+/CD45low cells, quadrant 2 (Q2) containing CD11b+/CD45hi cells and quadrant 3 (Q3) containing CD11bneg/CD45hi cells. The fourth quadrant contains the majority of retinal cells, which are CD11bneg/CD45neg. (B) Example of MHCII staining distributions of cells from quadrants 1, 2 and 3. Red traces are for cells from Sham-treated retinas, blue traces are for cells from IR retinas and the black trace is for cells incubated without the MHCII antibody and used to define the intensity cutoff between define MHCII+ and MHCIIneg cells. (C) Quantification of total events, MHCII+ events and MHCIIneg events from quadrants 1, 2 and 3 from Sham and IR retinas from PBS- and Mino-treated rats. The numbers shown are mean percentiles relative to all events, including those in quadrant 4, obtained from three separate flow experiments, with a total of 11 to 12 determinations per group. Significant differences between Sham and IR groups are indicated as *P ≤0.05, **P ≤0.01 and ***P ≤0.001, while significant effects of Mino-treatment are indicated by #P ≤0.05 and ##P ≤0.01. All comparisons are by Student's t-test.

From: Abcouwer SF, Lin CM, Shanmugam S, Muthusamy A, Barber AJ, Antonetti DA. Minocycline prevents retinal inflammation and vascular permeability following ischemia-reperfusion injury.
J Neuroinflammation. 2013 Dec 10;10:149.

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Mouse anti MHC Class II H-2I-Ak/s antibody, clone OX-6 used for the evaluation of microglial activation in rat brain using immunohistochemistry on
Effect of chronic stress on the lipopolysaccharide-induced activation of microglia in the ventral mesencephalon. Midbrain microglia were evaluated by immunohistochemistry with Iba-1 (left and middle columns) and OX-6 antibodies (right column) in vehicle-injected animals (A) through (F) and lipopolysaccharide (LPS)-injected animals (G) through (L) under nonstressed conditions ((A) through C) and (G) through (I)) and stressed conditions ((D) through (F) and (J) through (L)). Iba-1 immunohistochemistry is shown at low and high magnification (left and middle columns, respectively). (M) through (O) The effect of RU486 (mifepristone (11β-[p-(dimethylamino)phenyl]-17β-hydroxy-17-(1-propynyl)estra-4,9-dien-3-one)) on the microglia population in response to LPS injection in stressed animals. Note that stress highly increased the microglial activation response to LPS injection (J) through (L) compared with nonstressed conditions (G) through (I). Note that, after LPS injection, most microglial cells display a round morphology typical of macrophages, whose density significantly increases under conditions of chronic stress. Also note how RU486 treatment strongly prevents the stress-induced sensitisation of microglia to subsequent LPS injection (M) through (O). The blue staining in all panels is the Monastral Blue inert tracer contained in the vehicle. Scale bars: 500 μm (A, D, G, J and M); 100 µm (all other panels). Abbreviations: V, Vehicle; S, Stress; SL, Lipopolysaccharide injected into stressed animals; SLR, Lipopolysaccharide injected into stressed animals treated with RU486.

From: de Pablos RM, Herrera AJ, Espinosa-Oliva AM, Sarmiento M, Muñoz MF, Machado A, Venero JL. Chronic stress enhances microglia activation and exacerbates death of nigral dopaminergic neurons under conditions of inflammation. J Neuroinflammation. 2014 Feb 24;11:34.

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Mouse anti MHC Class II H-2I-Ak/s used for evaluation of MHCII expression on immune cells from rat brain by flow cytometry.
Image caption:
Experimental design. Representative scatter plots and gating strategies for immune cell analysis in brains from rats subjected to tMCAO. (A) Cells were first gated for lymphocytes (SSC-A versus FSC-A) and (B) singlets (FSC-W versus FSC-A). Thereafter, surface expression of (C) MHC class II or (D) CD11b was determined from this gated population. Abbreviations: forward side scatter (FSC); side scatter (SSC); fluorescence minus one (FMO).

From: Kuric E, Ruscher K. Dynamics of major histocompatibility complex class II-positive cells in the postischemic brain--influence of levodopa treatment.
J Neuroinflammation. 2014 Aug 23;11:145.

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Mouse anti MHC Class II H-2I-Ak/s antibody, clone OX-6 used for the identification of activated microglia in rat brain by immunohistochemistry on formalin fixed free-floating sections.
Image caption:Spatiotemporal expression of MHC II protein in the ischemic hemisphere. Spatiotemporal distribution of MHC II expressing cells in the ipsilateral cortex of a sham operated animal and at the indicated time points after tMCAO. The border of the infarct core is delineated by a dotted line. Scale bars: low magnification - 200 μm; higher magnification - 20 μm. Abbreviations: V, lateral ventricle; IC, infarct core.

From: Kuric E, Ruscher K. Dynamics of major histocompatibility complex class II-positive cells in the postischemic brain--influence of levodopa treatment.
J Neuroinflammation. 2014 Aug 23;11:145.

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Mouse anti MHC Class II H-2I-Ak/s antibody, clone OX-6 used for the demonstration of activated microglia in rat brain by immunofluorescence
Image caption:Reduction of MHC II protein and pro-inflammatory cytokines 14 days after tMCAO. (A) Co-localization of MHC II (Cy3, red) and D1 receptors (Alexa 488, green) in the peri-infarct area 14 days after tMCAO; two cells are indicated with an arrow. Higher magnification of the area delineated in the merged low power micrograph. The asterisk indicates the infarct core. Scale bars: low magnification - 50 μm; high magnification - 5 μm. (B) Western blot analysis for MHC II in the infarct core of rats treated either with vehicle (VH, n?=?4) or levodopa/benserazide (LD, n?=?4). Levels of MHC II protein are presented as arbitrary units (AU) to the right. Data are displayed as means?±?SEM, Student's t-test, P?<?0.05. (C) Levels of IFN-γ in the infarct core, (D) TNF-α and (E) IL-4 levels in the peri-infarct region from sham operated animals (VH n?=?4; LD n?=?4) and rats subjected to tMCAO (VH n?=?8; LD n?=?8). Results are presented as means?±?SEM. Statistical differences were tested with two-way ANOVA followed by Bonferroni post hoc correction or Fisher's Least Significant Difference test (italic). Letters denote P values; a) P?=?0.043, b) P?=?0.002, c) P?=?0.021. d) P?=?0.013, e) P?=?0.016, f) P?=?0.009, g) P?=?0.032, h) P?=?0.030, i) P?=?0.008. Abbreviations: M, marker lane (kDa); S, sham operated animals; VH, vehicle; LD, levodopa/benserazide.

From: Kuric E, Ruscher K. Dynamics of major histocompatibility complex class II-positive cells in the postischemic brain - influence of levodopa treatment.
J Neuroinflammation. 2014 Aug 23;11:145.

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Mouse anti MHC Class II H-2I-Ak/s antibody , clone OX-6 used for the identification of MHC class II expressing microglial cells in rat brain by immunohistochemistry on formalin fixed free foating sections.
Image caption:MHC II+cells in the corpus callosum (CC) after tMCAO. Temporal expression of MHC II in the CC contralateral and ipsilateral to the lesioned hemisphere at different time points after tMCAO and a sham operated animal, respectively. Scale bars: lower magnification - 100 μM; higher magnification - 20 μM.

From: Kuric E, Ruscher K. Dynamics of major histocompatibility complex class II-positive cells in the postischemic brain--influence of levodopa treatment.
J Neuroinflammation. 2014 Aug 23;11:145.

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Published customer image
Mouse anti MHC Class II H-2I-Ak/s used for the evaluation of MHC class II expressing cells in the rat corpus callosum by immunohistochemistry on formalin fixed free floating sections.
Image caption:Effect of levodopa/benserazide treatment on MHC II+cells in the corpus callosum (CC) after tMCAO. (A) Number of MHC II+ cells in the CC contralateral to the lesioned hemisphere of rats treated with vehicle (n?=?7) or levodopa/benserazide (n?=?6). Data are presented as means?±?SEM and statistical difference was tested with the Student's t-test, P?<?0.05. (B) Correlation between infarct volume presented in mm3 and the absolute number of MHC II+ cells. Abbreviations: VH, vehicle; LD, levodopa/benserazide.

From: Kuric E, Ruscher K. Dynamics of major histocompatibility complex class II-positive cells in the postischemic brain - influence of levodopa treatment.
J Neuroinflammation. 2014 Aug 23;11:145.

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  • Product Type
    Monoclonal Antibody
  • Clone
    OX-6
  • Isotype
    IgG1
5 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA2687FTFdatasheet pdfdatasheet pdf0.1 mg
    MCA2687FT
    MCA2687GAC, F, P *datasheet pdfdatasheet pdf0.1 mg
    MCA2687GA
    MCA2687RC, F, P *, WBdatasheet pdfdatasheet pdf0.25 mg
    MCA2687R
    MCA2687FBFdatasheet pdfdatasheet pdf0.5 mg
    MCA2687FB
    MCA2687PEFdatasheet pdfdatasheet pdf100 Tests
    MCA2687PE
    MCA2687A488Fdatasheet pdfdatasheet pdf100 Tests/1ml
    MCA2687A488
    MCA2687A647Fdatasheet pdfdatasheet pdf100 Tests/1ml
    MCA2687A647
    MCA2687FAFdatasheet pdfdatasheet pdf50 µg
    MCA2687FA
    MCA2687PEBFdatasheet pdfdatasheet pdf500 Tests
    MCA2687PEB
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
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    Reviews
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    • Mouse anti MHC Class II H-2I-Ak/s antibody, clone OX-6 recognizes a monomorphic determinant of the rat RT1B MHC class II antigen present on B lymphocytes, dendritic cells, some macrophages and certain epithelial cells.

      Mouse anti MHC Class II H-2I-Ak/s antibody, clone OX-6 does not react with the rat BDIX strain due to a defect in RT1B expression (Male, D. K. et al.).

      Mouse anti MHC Class II H-2I-Ak/s antibody, clone OX-6 also cross reacts with a polymorphic determinant on mouse strains of the H-2 haplotypes k and s. Analysis of recombinant mouse strains has mapped the OX-6 determinant to the H-2I-A region (McMaster & Williams 1979) and (Maleet al. 1987).

      The major histocompatibility complex (MHC) is a cluster of genes that are important in the immune response to infections. In mice, this complex is referred to as the H-2 region. In rats, this complex is referred to as the RT1 region.

      This product is routinely tested in flow cytometry on rat splenocytes.
    • Intended Use
    • Target Species
      Rat
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      Mouseyes
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
      Purified IgG conjugated to Alexa Fluor® 488 - liquid
      Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
      Purified IgG - liquid
      Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
      Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilised
      Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilised
      Purified IgG - liquid
      Purified IgG conjugated to Alexa Fluor® 647 - liquid
    • Reconstitution
      Pack Size: 100 TestsReconstitute with 1 ml distilled water
      Pack Size: 500 TestsReconstitute with 5.0 ml distilled water
      Pack Size: 100 TestsReconstitute with 1 ml distilled water
      Pack Size: 500 TestsReconstitute with 5.0 ml distilled water
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Pack Size: 0.1 mgPurified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Pack Size: 0.25 mgPurified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Pack Size: 100 TestsPurified IgG prepared by affinity chromatography on Protein G
      Pack Size: 500 TestsPurified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Pack Size: 100 TestsPurified IgG prepared by affinity chromatography on Protein G
      Pack Size: 500 TestsPurified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Pack Size: 0.1 mgPurified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Pack Size: 0.25 mgPurified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
    • Preservative Stabilisers
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      0.09%Sodium Azide
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      5%Sucrose
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      5%Sucrose
      0.09%Sodium Azide
      0.09%Sodium Azide
      1%Bovine Serum Albumin
    • Immunogen
      Rat thymocyte membrane glycoproteins.
    • Purity
    • Approx. Protein Concentrations
      Pack Size: 50 µg, 0.1 mgIgG concentration 0.1 mg/ml
      Pack Size: 0.5 mgIgG concentration 0.5 mg/ml
      IgG concentration 0.05 mg/ml
      Pack Size: 50 µg, 0.1 mgIgG concentration 0.1 mg/ml
      Pack Size: 0.5 mgIgG concentration 0.5 mg/ml
      IgG concentration 1.0 mg/ml
      Pack Size: 50 µg, 0.1 mgIgG concentration 0.1 mg/ml
      Pack Size: 0.5 mgIgG concentration 0.5 mg/ml
      IgG concentration 1.0 mg/ml
      IgG concentration 0.05 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
    • Fusion Partners
      Spleen cells from immunised Balb/c mice were fused with cells from the NS1 mouse myeloma cell line.
    • Storage
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.

      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.

      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.

      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.

      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Prior to reconstitution store at +4oC. Following reconstitution store at +4oC.

      DO NOT FREEZE.

      This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
      Prior to reconstitution store at +4oC. Following reconstitution store at +4oC.

      DO NOT FREEZE.

      This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.

      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch.
      12 months from date of reconstitution.
      12 months from date of reconstitution.
      18 months from date of despatch.
      18 months from date of despatch.
    • Acknowledgements
      This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
      This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry
      Neat Pack Size: 50 µg, 0.1 mg
      1/50 Pack Size: 0.5 mg
      1/10Pack Size: 50 µg, 0.1 mg
      1/100Pack Size: 0.5 mg
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat1/10
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry
      Neat Pack Size: 50 µg, 0.1 mg
      1/50 Pack Size: 0.5 mg
      1/10Pack Size: 50 µg, 0.1 mg
      1/100Pack Size: 0.5 mg
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1/501/100
      Immunohistology - Frozen
      Immunohistology - Paraffin(1)1/501/100
      Western Blotting
      (1)
      This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose. PLP fixation is recommended for optimal results
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry
      Neat Pack Size: 50 µg, 0.1 mg
      1/50 Pack Size: 0.5 mg
      1/10Pack Size: 50 µg, 0.1 mg
      1/100Pack Size: 0.5 mg
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat1/10
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat1/10
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1/501/100
      Immunohistology - Frozen
      Immunohistology - Paraffin(1)1/501/100
      Western Blotting
      (1)
      This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose. PLP fixation is recommended for optimal results
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat1/10

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Flow Cytometry
      Use 10ul of the suggested working dilution to label 106 cells in 100ul.
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use

    Additional MHC Class II H-2I-Ak/s Antibody Formats

    Formats Clone Applications Sizes available
    MHC Class II H-2I-Ak/s Antibody : RPE OX-6 F 500 Tests | 100 Tests
    MHC Class II H-2I-Ak/s Antibody : FITC OX-6 F 0.1 mg | 50 µg | 0.5 mg
    MHC Class II H-2I-Ak/s Antibody : Alexa Fluor® 488 OX-6 F 100 Tests/1ml
    MHC Class II H-2I-Ak/s Antibody : Purified OX-6 C, F, P *, WB 0.1 mg | 0.25 mg
    MHC Class II H-2I-Ak/s Antibody : Alexa Fluor® 647 OX-6 F 100 Tests/1ml
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

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      STAR117A
      Goat anti Mouse IgG/A/M:Alk. Phos.STAR87A1 mgC, E, WB
      STAR87A
      Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)STAR117D488GA0.1 mgF, IF
      STAR117D488GA
      Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed)STAR117D549GA0.1 mgF, IF
      STAR117D549GA
      Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed)STAR117D649GA0.1 mgF, IF
      STAR117D649GA
      Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)STAR117D680GA0.1 mgF, WB
      STAR117D680GA
      Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)STAR117D800GA0.1 mgF, IF, WB
      STAR117D800GA
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      STAR8D800GA
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      STAR117F
      Goat anti Mouse IgG:FITC (Rat Adsorbed)STAR700.5 mgF
      STAR70
      Goat anti Mouse IgG (Fc):FITCSTAR120F1 mgC, F
      STAR120F
      Rabbit F(ab')2 anti Mouse IgG:FITCSTAR9B1 mgF
      STAR9B
      Human anti Mouse IgG1:HRPHCA036P0.1 mgE
      HCA036P
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      STAR117P
      Goat anti Mouse IgG:HRP (Rat Adsorbed)STAR770.5 mgC, E, P
      STAR77
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      STAR120P
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      STAR13B
      Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)STAR87P1 mgE
      STAR87P
      Rabbit F(ab')2 anti Mouse IgG:RPESTAR12A1 mlF
      STAR12A
      Goat anti Mouse IgG:RPE (Rat Adsorbed)STAR761 mlF
      STAR76
      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)STAR117A0.5 mgE, WB
      STAR117A
      Goat anti Mouse IgG/A/M:Alk. Phos.STAR87A1 mgC, E, WB
      STAR87A
      Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)STAR117D488GA0.1 mgF, IF
      STAR117D488GA
      Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed)STAR117D549GA0.1 mgF, IF
      STAR117D549GA
      Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed)STAR117D649GA0.1 mgF, IF
      STAR117D649GA
      Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)STAR117D680GA0.1 mgF, WB
      STAR117D680GA
      Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)STAR117D800GA0.1 mgF, IF, WB
      STAR117D800GA
      Rabbit F(ab')2 anti Mouse IgG:Dylight®800STAR8D800GA0.1 mgF, IF, WB
      STAR8D800GA
      Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)STAR117F0.5 mgF
      STAR117F
      Goat anti Mouse IgG:FITC (Rat Adsorbed)STAR700.5 mgF
      STAR70
      Goat anti Mouse IgG (Fc):FITCSTAR120F1 mgC, F
      STAR120F
      Rabbit F(ab')2 anti Mouse IgG:FITCSTAR9B1 mgF
      STAR9B
      Human anti Mouse IgG1:HRPHCA036P0.1 mgE
      HCA036P
      Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)STAR117P0.5 mgE, WB
      STAR117P
      Goat anti Mouse IgG:HRP (Rat Adsorbed)STAR770.5 mgC, E, P
      STAR77
      Goat anti Mouse IgG (Fc):HRPSTAR120P1 mgE, WB
      STAR120P
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      STAR13B
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      STAR87P
      Rabbit F(ab')2 anti Mouse IgG:RPESTAR12A1 mlF
      STAR12A
      Goat anti Mouse IgG:RPE (Rat Adsorbed)STAR761 mlF
      STAR76

      Recommended Negative Isotype Control

        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Mouse IgG1 Negative Control:FITCMCA1209F0.1 mgF
        MCA1209F
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Mouse IgG1 Negative Control:Alexa Fluor® 488MCA1209A488100 Tests/1mlF
        MCA1209A488
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Mouse IgG1 Negative Control:FITCMCA1209F0.1 mgF
        MCA1209F
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Mouse IgG1 Negative ControlMCA12090.1 mgF
        MCA1209
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Mouse IgG1 Negative Control:FITCMCA1209F0.1 mgF
        MCA1209F
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Mouse IgG1 Negative Control:RPEMCA1209PE100 TestsF
        MCA1209PE
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Mouse IgG1 Negative Control:RPEMCA1209PE100 TestsF
        MCA1209PE
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Mouse IgG1 Negative ControlMCA12090.1 mgF
        MCA1209
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Mouse IgG1 Negative Control:Alexa Fluor® 647MCA1209A647100 Tests/1mlF
        MCA1209A647

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              • Application NameReference Images
                Flow Cytometry

              References

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