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OX-62 antibody | OX-62

Product Code Applications Pack Size List Price Your Price Qty
MCA1029G
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C F IP WB 0.25 mg loader
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loader
MCA1029GA
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C F IP 0.1 mg loader
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loader

Mouse anti Rat OX-62 antibody, clone OX-62 recognizes the OX-62 antigen, also known as rat alpha E integrin or CD103. OX-62 appears as an 1150 amino acid, ~130 kDa single pass type I transmembrane protein expressed by intestinal dendritic cells, dendritic epidermal T cells, intraepithelial lymphocytes in the small intestine and by cells of dendritic morphology in lymphoid organs, at sites where gamma delta T cells are present.

Target Species
Rat
Product Form
MCA1029G: Purified IgG - liquid
MCA1029GA: Purified IgG - liquid.
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% sodium azide (NaN3)
Carrier Free
Yes
Immunogen
Density gradient enriched PVG rat thoracic duct dendritic cells.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells of BALB/c mice were fused with cells of the mouse NS0 myeloma cell line.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/50 1/100
Immunohistology - Frozen 1/25 1/100
Immunoprecipitation
Western Blotting
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10μl of the suggested working dilution to label 106 cells in 100μl

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Description Mouse IgG1 Negative Control

Source Reference

  1. Brenan, M. & Puklavec, M. (1992) The MRC OX-62 antigen: a useful marker in the purification of rat veiled cells with the biochemical properties of an integrin.
    J Exp Med. 175 (6): 1457-65.

References for OX-62 antibody

  1. Chen-Woan, M. et al. (1995) A new protocol for the propagation of dendritic cells from rat bone marrow using recombinant GM-CSF, and their quantification using the mAb OX-62.
    J Immunol Methods. 178 (2): 157-71.
  2. Brenan, M. & Rees, D.J. (1997) Sequence analysis of rat integrin alpha E1 and alpha E2 subunits: tissue expression reveals phenotypic similarities between intraepithelial lymphocytes and dendritic cells in lymph.
    Eur J Immunol. 27 (11): 3070-9.
  3. Varas, A. et al. (1998) Interleukin-7 influences the development of thymic dendritic cells.
    Blood. 92: 93-100.
  4. Henry, F. et al. (1999) Antigen-presenting cells that phagocytose apoptotic tumor-derived cells are potent tumor vaccines.
    Cancer Res. 59: 3329-32.
  5. Aiello, S. et al. (2000) Thymic Dendritic Cells Express Inducible Nitric Oxide Synthase and Generate Nitric Oxide in Response to Self- and Alloantigens
    J Immunol. 164: 4649-58.
  6. O'Sullivan, N.L. et al. (2001) Lymphocyte lineages at mucosal effector sites: rat salivary glands.
    J Immunol. 166: 5522-9.
  7. Kostulas, N. et al. (2002) Dendritic cells are present in ischemic brain after permanent middle cerebral artery occlusion in the rat.
    Stroke. 33 (4): 1129-34.
  8. Tsuchiya, T. et al. (2002) Dendritic cell involvement in pulmonary granuloma formation elicited by bacillus calmette-guérin in rats.
    Am J Respir Crit Care Med. 165 (12): 1640-6.
  9. View The Latest Product References
  10. Camelo, S. et al. (2003) Local retention of soluble antigen by potential antigen-presenting cells in the anterior segment of the eye.
    Invest Ophthalmol Vis Sci. 44: 5212-9.
  11. Penttila, I.A. et al. (2003) Maternal milk regulation of cell infiltration and interleukin 18 in the intestine of suckling rat pups.
    Gut. 52: 1579-86.
  12. Camelo, S. et al. (2004) The distribution of antigen in lymphoid tissues following its injection into the anterior chamber of the rat eye.
    J Immunol. 172: 5388-95.
  13. Sugawara, I. et al. (2004) Pathological and immunological profiles of rat tuberculosis.
    Int J Exp Pathol. 85: 125-34.
  14. Stephens, L.A. et al. (2004) Phenotypic characterization of regulatory CD4+CD25+ T cells in rats.
    Int Immunol. 16: 365-75.
  15. Rival, C. et al. (2006) Identification of a dendritic cell population in normal testis and in chronically inflamed testis of rats with autoimmune orchitis.
    Cell Tissue Res. 324 (2): 311-8.
  16. Baca Jones, C.C. et al. (2009) Rat cytomegalovirus infection depletes MHC II in bone marrow derived dendritic cells.
    Virology. 388: 78-90.
  17. Tiurbe, G. et al. (2009) Inhibitory effects of rat bone marrow-derived dendritic cells on naïve and alloantigen-specific CD4+ T cells: a comparison between dendritic cells generated with GM-CSF plus IL-4 and dendritic cells generated with GM-CSF plus IL-10.
    BMC Res Notes. 2: 12.
  18. Zilka, N. et al. (2009) Human misfolded truncated tau protein promotes activation of microglia and leukocyte infiltration in the transgenic rat model of tauopathy.
    J Neuroimmunol. 209 (1-2): 16-25.
  19. Schwartzkopff, J. et al. (2010) NK cell depletion delays corneal allograft rejection in baby rats.
    Mol Vis. 16: 1928-35.
  20. Zhou, Y.J. et al. (2010) The role of the lactadherin in promoting intestinal DCs development in vivo and vitro.
    Clin Dev Immunol. 2010: 357541.
  21. Bufan, B. et al. (2015) Aging Impairs Endocytic Capacity Of Splenic Dendritic Cells From Dark Agouti Rats And Alters Their Response To TLR4 Stimulation
    Acta Veterinaria. Volume 65, Issue 1, Pages 30–55.
  22. Syrjälä, S. et al. (2015) Donor Heart Treatment With COMP-Ang1 Limits Ischemia-Reperfusion Injury and Rejection of Cardiac Allografts.
    Am J Transplant. 15 (8): 2075-84.
  23. Stojić-Vukanić, Z. et al. (2016) Estradiol enhances capacity of TLR-matured splenic dendritic cells to polarize CD4+ lymphocytes into IL-17/GM-CSF-producing cells in vitro.
    Int Immunopharmacol. 40: 244-253.
  24. Palin, N.K. et al. (2017) Activin inhibition limits early innate immune response in rat kidney allografts-a pilot study.
    Transpl Int. 30 (1): 96-107.
  25. Raissadati, A. et al. (2017) Vascular Endothelial Growth Factor-B Overexpressing Hearts Are Not Protected From Transplant-Associated Ischemia-Reperfusion Injury.
    Exp Clin Transplant. 15 (2): 203-12.
  26. Stojić-Vukanić, Z. et al. (2020) Age and sex determine CD4+ T cell stimulatory and polarizing capacity of rat splenic dendritic cells.
    Biogerontology. 21 (1): 83-107.
  27. Pilipović, I. et al. (2019) Noradrenaline modulates CD4+ T cell priming in rat experimental autoimmune encephalomyelitis: a role for the α1-adrenoceptor.
    Immunol Res. 67 (2-3): 223-40.
  28. Lauzon-Joset, J.F. et al. (2020) Oestrogen amplifies pre-existing atopy-associated Th2 bias in an experimental asthma model.
    Clin Exp Allergy. 50 (3): 391-400.
  29. Yu, J. et al. (2022) Topical Administration of 0.3% Tofacitinib Suppresses M1 Macrophage Polarization and Allograft Corneal Rejection by Blocking STAT1 Activation in the Rat Cornea
    Transl Vis Sci Technol. 11 (3): 34
  30. Cąkała-Jakimowicz, M. & Puzianowska-Kuznicka, M. (2022) Towards Understanding the Lymph Node Response to Skin Infection with Saprophytic Staphylococcus epidermidis..
    Biomedicines. 10 (5): 1021.
  31. de Jesus, E.J. et al. (2016) Adoptive Transfer of Dendritic Cells Expressing Fas Ligand Modulates Intestinal Inflammation in a Model of Inflammatory Bowel Disease.
    J Clin Cell Immunol. 7 (2): 411.
  32. Leffler, J. et al. (2018) Functional differences in airway dendritic cells determine susceptibility to IgE-sensitization.
    Immunol Cell Biol. 96 (3): 316-29.
  33. Yu, J. et al. (2021) Subconjunctival injections of dimethyl fumarate inhibit lymphangiogenesis and allograft rejection in the rat cornea.
    Int Immunopharmacol. 96: 107580.
  34. Djuretić, J. et al. (2019) Natural killer cells as participants in pathogenesis of rat experimental autoimmune encephalomyelitis (EAE): lessons from research on rats with distinct age and strain.
    Cent Eur J Immunol. 44 (4): 337-356.

Flow Cytometry

Immunocytochemistry

Immunohistology - Frozen

Synonyms
CD103
RRID
AB_2128733
UniProt
O88341

MCA1029G

1804

MCA1029GA

149732 158691

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