Bevacizumab antibody | AbD17976_hIgG1

Human anti Bevacizumab

Product Type
Monoclonal Antibody
Clone
AbD17976_hIgG1
Isotype
IgG1
Specificity
Bevacizumab

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HCA185G
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SDS Safety Datasheet SDS
E 1 mg loader
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HCA185
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Human Anti-Bevacizumab Antibody, clone AbD17976_hIgG1 is an affinity-matured, inhibitory, anti-idiotypic antibody that recognizes the humanized monoclonal antibody drugs bevacizumab and ranibizumab, and their biosimilar products.

Clone AbD17976_hIgG1 is a fully human recombinant monoclonal antibody with IgG1 isotype and is suitable as a positive control or calibrator in an anti-drug antibody (ADA) assay.

This Anti-Bevacizumab Antibody cross-reacts with the drug ranibizumab (Lucentis). Ranibizumab is a humanized Fab fragment antibody (VH3-23/kappa1), originating from the same parental mouse antibody as bevacizumab; it has 4 amino acid exchanges in the heavy chain and 1 amino acid exchange in the light chain compared to bevacizumab.

Bevacizumab (trade name Avastin) is a humanized monoclonal antibody (IgG1/kappa) that inhibits angiogenesis by binding to vascular endothelial growth factor alpha (VEGF-A). VEGF-A is a potent stimulator of angiogenesis of both normal and cancerous cells, and acts as a regulator of vasculogenesis. Bevacizumab is used to treat various cancers, including colorectal, lung, breast, glioblastoma, kidney and ovarian.

View a summary of all anti-bevacizumab antibodies.

Product Form
Pack Size: 1 mg
Human IgG1 antibody selected from the HuCAL® phage display library and expressed in a human cell line. This antibody is supplied as a liquid.
Pack Size: 0.1 mg
Human IgG1 antibody selected from the HuCAL? phage display library and expressed in a human cell line. This antibody is supplied as a liquid.
Preparation
Purified IgG prepared by affinity chromatography on Protein A
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.01% Thiomersal
Immunogen
Bevacizumab
Affinity
The monovalent intrinsic affinity of this antibody was measured as KD=0.4 nM by real time, label-free molecular interaction analysis on immobilized bevacizumab.
Approx. Protein Concentrations
Pack Size: 1 mg
Total protein concentration 1.0 mg/ml
Pack Size: 0.1 mg
IgG concentration 1.0 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch
Acknowledgements
This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See bio-rad.com/en-us/trademarks for details.
Avastin is a trademark of Genentech Inc.

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Technical Advice
Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
ELISA
This product is suitable for use in direct ELISA applications.
This antibody is fully human and can be used as a reference standard in an ADA assay.
Protocol: ADA bridging ELISA

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References for Bevacizumab antibody

  1. Iwamoto, N. et al. (2018) Antibody drug quantitation in coexistence with anti-drug antibodies on nSMOL bioanalysis.
    Anal Biochem. 540-541: 30-7.

Synonyms
Avastin
Licensed Use
For in vitro research purposes and for commercial applications for the provision of in vitro testing services to support preclinical and clinical drug development. Any re-sale in any form or any other commercial application needs a written agreement with Bio-Rad.
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"