Ranibizumab antibody | AbD16746_hIgG1

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Human anti Ranibizumab

Human Anti-Ranibizumab Antibody is a paratope specific, inhibitory anti-idiotypic antibody in IgG1 format that recognizes the monoclonal antibody drugs ranibizumab and bevacizumab, and their biosimilars. This antibody can be used as a control or calibrator in an anti-drug antibody assay.

Product Type
Monoclonal Antibody
Clone
AbD16746_hIgG1
Isotype
IgG1
Product Code Applications Pack Size List Price Quantity
HCA305
Datasheet
E
MSDS
0.1 mg loader

Human Anti-Ranibizumab Antibody, clone AbD16746_hIgG1 is a paratope specific, inhibitory anti-idiotypic antibody in IgG1 format that recognizes the monoclonal antibody drug ranibizumab. The antibody does not recognize recombinant human vascular endothelial growth factor A (VEGF-A) or ranibizumab when in complex with recombinant human VEGF-A.

Clone AbD16746_hIgG1 is a fully human recombinant monoclonal antibody with IgG1 isotype and is suitable as a control or calibration standard in an anti-drug antibody (ADA) assay.

This antibody also recognizes the monoclonal antibody drug bevacizumab.

Ranibizumab (Lucentis) is a humanized Fab fragment antibody (VH3-23/kappa1), originating from the same parental mouse antibody as bevacizumab (Avastin); it has 4 amino acid exchanges in the heavy chain and 1 amino acid exchange in the light chain compared to bevacizumab. Ranibizumab was first approved to treat wet age-related macular degeneration (AMD), a common form of age-related vision loss. It is also approved for the treatment of macular edema following retinal vein occlusion, diabetic macular edema, and diabetic retinopathy. Ranibizumab inhibits angiogenesis by binding to VEGF-A, blocking its interaction with VEGF receptors on the surface of endothelial cells, and thus preventing the subsequent growth of new blood vessels.

View a summary of all anti-ranibizumab antibodies

Product Details

Product Form
Human IgG1 antibody (lambda light chain) selected from the HuCAL® phage display library and expressed in a human cell line. This antibody is supplied as a liquid.
Preparation
Purified IgG prepared by affinity chromatography on Protein A
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.01% Thiomersal
Immunogen
Ranibizumab
Affinity
The monovalent intrinsic affinity of AbD16746_hIgG1 was measured as KD= 2 nM by real time, label free molecular interaction analysis on immobilized bevacizumab.
Approx. Protein Concentrations
Antibody concentration 0.5 mg/ml

Storage Information

Storage
+4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch.

More Information

Acknowledgements
Sold under license of U.S. Patents 6753136, 7785859 and 8273688 and corresponding patents. This antibody was developed by Bio-Rad, Zeppelinstr. 4, 82178 Puchheim, Germany. Lucentis is a trademark of Genentech.
Licensed Use
For in vitro research purposes and for commercial applications for the provision of in vitro testing services to support preclinical and clinical drug development. Any re-sale in any form or any other commercial application needs a written agreement with Bio-Rad.
Regulatory
For research purposes only

Applications of Ranibizumab antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Technical Advice
Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
ELISA
Clone AbD16746_hIgG1 can be used in a direct or indirect ELISA or as a calibrator for the set-up of an anti-drug antibody assay.

Protocol: ADA bridging ELISA

Useful Reagents Available

Description Product Code Applications Pack Size List Price Quantity
Hispec Assay Diluent BUF049A E IY 50 ml
LYNX Rapid HRP Antibody Conjugation Kit LNK002P CJ 3 Conjugations For 400µg Antibody