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SLA Class II DR antibody | 2E9/13

Mouse anti Pig SLA Class II DR

Product Type
Monoclonal Antibody
Clone
2E9/13
Isotype
IgG2b
Specificity
SLA Class II DR

Product Code Applications Pack Size List Price Your Price Qty
MCA2314GA
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C F IP 0.1 mg loader
List Price Your Price
loader

Mouse anti Pig SLA Class II DR antibody, clone 2E9/13 recognizes SLA DR molecules which are expressed on all B cells, antigen presenting cells and on certain subsets of resting and activated T cells. Mouse anti Pig SLA Class II DR antibody, clone 289/13 reacts with lymphocytes from all outbred and miniature pigs so far tested, suggesting that it recognizes a monomorphic determinant of porcine SLA DR.

The major histocompatibility complex (MHC) is a cluster of genes that are important in the immune response to infections. In pigs, this is referred to as the swine leukocyte antigen (SLA) region. There are 3 major MHC class II proteins encoded by the SLA which are SLA DP, SLA DQ and SLA DR.

Mouse anti pig SLA class II DR, clone 2E9/13 immunoprecipitates a heterodimer composed of two polypeptides of ~28 and ~35 kDa from NP-40 extracts of biotin surface-labeled porcine peripheral blood mononuclear cells. Mouse anti Pig SLA Class II DR antibody, clone 289/13 is reported to inhibit the mixed lymphocyte reaction and T cell stimulation induced by African swine fever virus and staphylococcal enterotoxin B (Bullido et al. 1997).

Target Species
Pig
Species Cross-Reactivity
Target SpeciesCross Reactivity
Bovine
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% sodium azide (NaN3)
Carrier Free
Yes
Immunogen
Porcine monocytes.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunized BALB/c mice were fused with cells of the mouse X63-Ag.8.653 myeloma cell line.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/25 1/200
Immunohistology - Frozen
Immunoprecipitation
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10μl of the suggested working dilution to 1x106 cells in 100μl

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References for SLA Class II DR antibody

  1. Bullido, R. et al. (1997) Characterization of five monoclonal antibodies specific for swine class II major histocompatibility antigens and crossreactivity studies with leukocytes of domestic animals.
    Dev Comp Immunol. 21 (3): 311-22.
  2. Jeong, H.J. et al. (2010) Comparative measurement of cell-mediated immune responses of swine to the M and N proteins of porcine reproductive and respiratory syndrome virus.
    Clin Vaccine Immunol. 17: 503-12.
  3. Ding, Q. et al. (2011) Human PD-L1-overexpressing porcine vascular endothelial cells induce functionally suppressive human CD4+CD25hiFoxp3+ Treg cells.
    J Leukoc Biol. 90 (1): 77-86.
  4. Thierry, A. et al. (2012) Identification of invariant natural killer T cells in porcine peripheral blood.
    Vet Immunol Immunopathol. 149 (3-4): 272-9.
  5. Iwase H et al. (2015) Initial in vivo experience of pig artery patch transplantation in baboons using mutant MHC (CIITA-DN) pigs.
    Transpl Immunol. 32 (2): 99-108.
  6. Zanotti, C. et al. (2015) Differential Biological Activities of Swine Interferon-α Subtypes.
    J Interferon Cytokine Res. 35 (12): 990-1002.
  7. Wang, Y. et al. (2016) Genipin crosslinking reduced the immunogenicity of xenogeneic decellularized porcine whole-liver matrices through regulation of immune cell proliferation and polarization.
    Sci Rep. 6: 24779.
  8. Gardner, D.S. et al. (2016) Remote effects of acute kidney injury in a porcine model.
    Am J Physiol Renal Physiol. 310 (4): F259-71.
  9. View The Latest Product References
  10. Singleton, H. et al. (2016) Establishing Porcine Monocyte-Derived Macrophage and Dendritic Cell Systems for Studying the Interaction with PRRSV-1.
    Front Microbiol. 7: 832.
  11. Rahe, M.C. & Murtaugh, M.P. (2017) Interleukin-21 Drives Proliferation and Differentiation of Porcine Memory B Cells into Antibody Secreting Cells.
    PLoS One. 12 (1): e0171171.
  12. Mašek, J. et al. (2017) Multi-layered nanofibrous mucoadhesive films for buccal and sublingual administration of drug-delivery and vaccination nanoparticles - important step towards effective mucosal vaccines.
    J Control Release. 249: 183-95.
  13. Ladowski, J.M. et al. (2018) Swine Leukocyte Antigen Class II Is a Xenoantigen.
    Transplantation. 102 (2): 249-54.
  14. Yang, N. et al. (2018) Reduced antigen presentation capability and modified inflammatory/immunosuppressive cytokine expression of induced monocyte-derived dendritic cells from peripheral blood of piglets infected with porcine circovirus type 2.
    Arch Virol. 163 (5): 1231-9.
  15. López, E. et al. (2019) Identification of very early inflammatory markers in a porcine myocardial infarction model.
    BMC Vet Res. 15 (1): 91.
  16. Liu, S. et al. (2019) Endothelial IL-8 induced by porcine circovirus type 2 affects dendritic cell maturation and antigen-presenting function.
    Virol J. 16 (1): 154.
  17. Radlowski, E.C. et al. (2021) Combination-Feeding Causes Differences in Aspects of Systemic and Mucosal Immune Cell Phenotypes and Functions Compared to Exclusive Sow-Rearing or Formula-Feeding in Piglets.
    Nutrients. 13(4):1097.
  18. Arenal, Á. et al. (2022) Effects of Cardiac Stem Cell on Postinfarction Arrhythmogenic Substrate.
    Int J Mol Sci. 23 (24): 16211.
  19. Franzoni, G. et al. (2022) Analyses of the Impact of Immunosuppressive Cytokines on Porcine Macrophage Responses and Susceptibility to Infection to African Swine Fever Viruses.
    Pathogens. 11 (2): 166.
  20. Haach, V. et al. (2023) A polyvalent virosomal influenza vaccine induces broad cellular and humoral immunity in pigs.
    Virol J. 20 (1): 181.
  21. Skirecki, T. et al. (2022) Compartment-Specific Differences in the Activation of Monocyte Subpopulations Are Not Affected by Nitric Oxide and Glucocorticoid Treatment in a Model of Resuscitated Porcine Endotoxemic Shock.
    J Clin Med. 11 (9): 2641.

Further Reading

  1. Piriou-Guzylack, L. (2008) Membrane markers of the immune cells in swine: an update.
    Vet Res. 39: 54.
  2. Rayat GR et al. (2016) First update of the International Xenotransplantation Association consensus statement on conditions for undertaking clinical trials of porcine islet products in type 1 diabetes - Chapter 3: Porcine islet product manufacturing and release testing criteria.
    Xenotransplantation. 23 (1): 38-45.

Flow Cytometry

UniProt
Q85ZW4

MCA2314GA

147544 154296 161441

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