CD68 Antibody | FA-11

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CD68 Antibody | FA-11 gallery image 1

Flow cytometric staining of mouse peritoneal macrophages cells with Rat anti Mouse CD68 (MCA1957) following permeablisation with Leucoperm (BUF09), visualised with Mouse adsorbed FITC conjugated F(ab')2 Goat anti Rat IgG: (STAR69)

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Western Blot analysis of CD68 expression on J774 cells using Rat anti Mouse CD68 (MCA1957GA) with Goat anti Rat IgG:HRP (STAR72) as a detection antibody

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Immunoperoxidase staining of mouse spleen cryosection with Rat anti Mouse CD68 (MCA1957) followed by Goat anti Rat IgG:HRP (305005) showing staining of macrophages in the red pulp

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Frozen mouse lymph node stained with rat anti-mouse CD68 (MCA1957) at a 1/100 dilution followed by goat anti-rat IgG:HRP (STAR72) at a 1/50 dilution.

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Frozen mouse lymph node stained with rat anti-mouse CD68 (MCA1957) at a 1/100 dilution followed by goat anti-rat IgG:HRP (STAR72) at a 1/50 dilution.

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Immunoperoxidase staining of a mouse lymph node cryosection with Rat anti Mouse CD68 antibody, clone FA11 (MCA1957) followed by Goat anti Rat IgG antibody (STAR72). High power

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Immunofluorescence staining of a mouse lymph node cryosection with Rat anti Mouse CD68 antibody, clone FA11 (MCA1957), green in A and Rat anti Mouse CD8 antibody, clone YTS105.18 (MCA1108), red in B. C is the merged image with nuclei counterstained blue using DAPI. Low power

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CD68 Antibody | FA-11 gallery image 8

Immunofluorescence staining of a mouse lymph node cryosection with Rat anti Mouse CD68 antibody, clone FA11 (MCA1957), green in A and Rat anti Mouse CD8 antibody, clone YTS105.18 (MCA1108), red in B. C is the merged image with nuclei counterstained blue using DAPI. Medium power

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CD68 Antibody | FA-11 gallery image 9

Immunofluorescence staining of a mouse lymph node cryosection with Rat anti Mouse CD68 antibody, clone FA11 (MCA1957), green in A and Rat anti Mouse CD8 antibody, clone YTS105.18 (MCA1108), red in B. C is the merged image with nuclei counterstained blue using DAPI. High power

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CD68 Antibody | FA-11 gallery image 10

Dot Blot showing FSC/SSC gated mouse peritoneal macrophages dual stained with CD68 (MCA1957A488) at a 1/5 dilution and CD88 (MCA2456A647) at a 1/5 dilution. Isotype control pair in red. Fc receptors were blocked by mouse Seroblock (BUF041B). Permeabilisation was with Leucoperm (BUF09)

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Rat anti Mouse CD68 antibody, clone FA-11 used for the identification of microglia in mouse brain by immunofluorescence.
Image caption:
CB2 receptors are expressed in microglial cells and do not accumulate in Aβ plaques of APPswe/PS1ΔE9 mice. A representative confocal image of staining for CB2 receptors (green, H60 antibody) in the cortex of 12 mo-old transgenic mice. (B) An overlap of red (CD68) and blue (DAPI) channels for the image shown in A. Note a characteristic gathering of activated microglia around an amyloid plaque (marked by an asterisk). (C) An overlap of channels shown in A-B. Note that areas with high CB2 intensities overlap with CD68-positive areas. White rectangle shows an example of areas used for quantifications presented in E-F. Scale bar is 15 μm. (D) Quantification of CB2 densities (integrated intensities/area) in CD68-positive and –negative areas. 26 areas like that shown in A-C were used for the quantification (n = 2 transgenic mice). Asterisk indicates a significant difference between CD68+ and CD68- areas (one-way ANOVA, p<0.0001). (E) A scatterplot of CB2 and DAPI intensities as a function of distance from the center of an Aβ plaque with radius ~10 µm. Note low CB2 signal in the core of the plaque. CB2 and DAPI intensities were normalized (%) to a maximum signal on each channel. An example of an area used for calculations is shown by a white rectangle in C. (F) Quantification of CB2 signal at different distances from a plaque center. 4–6 slices of z stacks from five plaques (range of radiuses 7–15 μm) were used in one-way ANOVA. Asterisks indicate a significant increase (p<0.0001, post-hoc test) in CB2 intensities as compared to the core of plaques (radius = 7 μm).

From: Savonenko AV, Melnikova T, Wang Y, Ravert H, Gao Y, Koppel J, et al. (2015) Cannabinoid CB2 Receptors in a Mouse Model of Aβ Amyloidosis: Immunohistochemical Analysis and Suitability as a PET Biomarker of Neuroinflammation.
PLoS ONE 10(6): e0129618.

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Rat anti Mouse CD68 antibody, clone FA-11 used for the identification of microglia in mouse brain by immunofluorescence.
Image caption:
Localization of CB2 receptors in microglial cells and engulfment synapses. (A) A confocal image of CB2 (green) and CD68 staining (red) centered at the core of an Aβ plaque (marked by an asterisk). Note a region of high intensity for CB2 and CD68 staining around the plaque implying that CB2 receptors are localized in microglial processes surrounding the plaque. Scale 15 μm. (B) A magnification of a microglia cell body (red) and its process (red) forming an engulfment synapse on a dense core amyloid plaque (marked by an asterisk, DAPI). Note CB2 receptor staining along the edge of CD68-positive staining and at the engulfment synapse. White boxes indicate areas used for quantifications in C-D. (C-D) Quantification of CB2, CD68, and DAPI signals from the image of a microglia cell body (C) and engulfment synapse (D). The quantification was done using a Plot profile analysis tool (Fiji). Signals were averaged along short axes of boxes shown in B and normalized to a max value (100%) for each channel. Units of X axis are pixels, scale: 11.1 pixels/ μm. (E) 3D reconstruction of the engulfment synapse shown in B, D. A z stack of 0.31 μm slices (n = 29) was processed by using a background subtraction function and normalization for each of the channels. Surfaces for the plaque (DAPI), microglia process (CD68), and CB2 signal were created by arbitrary thresholding at an upper third of intensity distributions. Note high intensities of CB2 signals are located between CD68 and DAPI surfaces. Insert shows orientation of a 3D window as related to a position of the brain slide.

From: Savonenko AV, Melnikova T, Wang Y, Ravert H, Gao Y, Koppel J, et al. (2015) Cannabinoid CB2 Receptors in a Mouse Model of Aβ Amyloidosis: Immunohistochemical Analysis and Suitability as a PET Biomarker of Neuroinflammation.
PLoS ONE 10(6): e0129618.

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CD68 Antibody | FA-11 gallery image 13

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Rat anti Mouse CD68 antibody, clone FA-11 used for the identification of microglia in mouse brain by immunofluorescence.
Image caption:
Comparison of CB2 immunoreactivity in neurons, activated microglia and astrocytes. (A) Representative confocal images from the cortex of 12 mo-old non-transgenic (NTG) and APPswe/PS1ΔE9 transgenic (AD) mice stained with a CB2 receptor antibody (H60sc; left columns; green) and markers for neurons (NeuN, far red), activated microglia (CD68, red), and astrocytes (GFAP, far red). Brain slides were counterstained with DAPI shown with a grey pseudo color. Note substantial micro- and astro-gliosis in the cortex of the AD mouse brain. In the NTG mice, CD68+ and/or GFAP+ areas were rare (indicated in the upper right panel by an arrowhead and arrow, respectively). (B) Quantification of densities (+-SEM) for CB2 receptor immunoreactivity (integrated intensities/area) in areas positive for NeuN, CD68, and GFAP markers. Densities were averaged over 22 (AD) and 14 (NTG) images of the cortex as shown in A (n = 2 mice per genotype). Single and double asterisks indicate a significant difference between NTG and AD groups as a result of LSD post-hoc test with p levels <0.01 and 0.0001, respectively. Arcs indicate non-significant (NS) differences. Single and double pound signs (p levels <0.05 and 0.001) indicate markers that correspond to the highest CB2 density in the NTG (blue sign) or AD (red sign) groups (LSD post-hoc test). Solid black line at the level of 4,930 shows average densities for the background. (C) An example of NeuN (blue), CD68 (red), and GFAP (green) masks from the AD image shown in A. NeuN masks were drawn by hand as shown in Fig 2C; masks for CD68 and GFAP were created by a threshold function. Black area represents background. Scale is 15 μm.

From: Savonenko AV, Melnikova T, Wang Y, Ravert H, Gao Y, Koppel J, et al. (2015) Cannabinoid CB2 Receptors in a Mouse Model of Aβ Amyloidosis: Immunohistochemical Analysis and Suitability as a PET Biomarker of Neuroinflammation.
PLoS ONE 10(6): e0129618.

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Mouse anti CD68 antibody, clone FA-11 used for the detection of macrophages in mouse brain by immunohistochemistry on cryostat sections.
Image caption:
Primed innate immune response in the brain following a systemic bacterial infection. Phenotypic changes after intracerebral injection of 100 pg of LPS in naïve mice (A, B, E, F, I, J, M, N) or mice pretreated with SL3261 (C, D, G, H, K, L, O, P). Representative images of immune marker expression (CD11c (A-D), CD68 (E-H), MHCII (I-L) in the injected hemisphere (B, D, F, H, J, L, N, P) or contralateral site are shown. Panels M-P show a double immunofluorescent stain for MHCII (green) and laminin (red). n=5 animals per group, black scale bar=50μm, white scale bar=75μm.

From: Püntener U, Booth SG, Perry VH, Teeling JL. Long-term impact of systemic bacterial infection on the cerebral vasculature and microglia.
J Neuroinflammation. 2012 Jun 27;9:146.

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Mouse anti CD68 antibody, clone FA-11 used for the detection of macrophages in mouse by flow cytometry.
Image caption:
F4/80 and either CD11b or CD68 expression of whole liver MNCs. Liver MNCs were obtained from CD, HFD, HCD and HFCD mice and expression of F4/80 either with CD11b or CD68 were examined. The numbers were the means±SE from four mice in each group. F4/80 positive gate was determined by using the isotype control Ab.

From: Nakashima H, Ogawa Y, Shono S, Kinoshita M, Nakashima M, et al. (2013) Activation of CD11b+ Kupffer Cells/Macrophages as a Common Cause for Exacerbation of TNF/Fas-Ligand-Dependent Hepatitis in Hypercholesterolemic Mice. PLoS ONE 8(1): e49339.

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Mouse anti CD68 antibody, clone FA-11 used for the detection of macrophages in mouse by flow cytometry.
Image caption:
The expression of CD68 and CD11b by liver F4/80+ Kupffer cells. Liver MNCs were obtained from CD, HFD, HCD and HFCD mice, and Kupffer cells gated by F4/80 were analyzed for their expression levels of CD68 and CD11b. CD68 positive gate was determined by using the isotype control Ab. The numbers are the means±SE from six to eight mice in each group. *P<0.05 vs. CD and HFD.

From: Nakashima H, Ogawa Y, Shono S, Kinoshita M, Nakashima M, et al. (2013) Activation of CD11b+ Kupffer Cells/Macrophages as a Common Cause for Exacerbation of TNF/Fas-Ligand-Dependent Hepatitis in Hypercholesterolemic Mice. PLoS ONE 8(1): e49339.

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Mouse anti CD68 antibody, clone FA-11 used for the detection of macrophages in mouse aortic root tissue by immunohistochemistry on cryostat sections.
Image caption:
Immunohistochemical analysis of mouse atherosclerotic lesions. Representative aortic root sections immunostained for the foam cell marker CD68 (A), VCAM-1 (B), or Masson trichrome blue stain for collagen content (C). Original magnification, 40×. Note abundant immunostaining for foam cell marker, CD68 (brown), VCAM-1 adhesion molecules (also brown), and presence of collagen ablue) within lesion in baseline and AAV.TBG.nLacZ injected Ldlr-/-Apobec1-/-animals.

From: Kassim SH, Li H, Vandenberghe LH, Hinderer C, Bell P, et al. (2010)
Gene Therapy in a Humanized Mouse Model of Familial Hypercholesterolemia Leads to Marked Regression of Atherosclerosis.
PLoS ONE 5(10): e13424.

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Mouse anti CD68 antibody, clone FA-11 used for the detection of macrophages in mouse by flow cytometry.
Image caption:
Phenotype of pancreatic macrophages. (A) Identification of macrophage subsets in pancreas of CX3CR1GFP/+ C56BL/6 mice. Pancreatic single cell suspensions were gated on FSC-A vs FSC-W and CD45+. Histograms show receptor expression profile of CX3CR1hiLYVE-1- (green line) and CX3CR1loLYVE-1+ (red line) macrophages. (B) Sorted CX3CR1loLYVE-1+ and CX3CR1hiLYVE-1- macrophages spun onto glass slides and stained with Wright's stain. 1000× magnification. (C) mRNA expression profile of chemokine receptors, chemokines and VEGFs in pancreatic macrophage subsets. mRNA levels examined by qRT-PCR in duplicate or triplicate. 6–7 mice/group, data representative of 2–4 separate experiments.

From: Yin N, Zhang N, Lal G, Xu J, Yan M, et al. (2011)
Lymphangiogenesis Is Required for Pancreatic Islet Inflammation and Diabetes.
PLoS ONE 6(11): e28023.

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Mouse anti CD68 antibody, clone FA-11 used for the detection of macrophages in mouse pancreas by immunofluorescence.
Image caption:
Interaction of LEC and pancreatic macrophages. (A) CX3CR1hi macrophages, LYVE-1+ macrophages, CD11b--lymphocytes and LEC were sorted from pancreatic single cell suspensions of CX3CR1GFP/+ mice. Co-cultured CX3CR1hi macrophages-CFSE, LYVE-1+ macrophages-eFlour670, or lymphocytes-CFSE with/without LEC on Matrigel for 5 days. Scale bars: 60 μm. 100× magnification. (B) Immunofluorescent staining of CD68 and LYVE-1 in pancreas from C57BL/6 mice. Scale bars: 160 μm. 50× magnification. (C) Sorted CX3CR1hi macrophages labeled with CFSE and co-cultured with LEC (left upper panel) or without LEC (left lower panel) for 5 days, and stained for CD11b (red) and LYVE-1 (yellow). Scale bars: 10 μm. 630× magnification. Right panel, quantitative analysis, total cells from 5 fields (1344×1024 pixels) were counted. All data representative of 2 to 4 separate experiments.

From: Yin N, Zhang N, Lal G, Xu J, Yan M, et al. (2011)
Lymphangiogenesis Is Required for Pancreatic Islet Inflammation and Diabetes.
PLoS ONE 6(11): e28023.

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Mouse anti CD68 antibody, clone FA-11 used for the detection of macrophages in mouse pancreas by immunofluorescence.
Image caption:
Macrophages infiltrate into inflamed islets. MLDS treated BALB/c mice received sunitinib, or anti-VEGFR3 mAb starting from the first STZ injection 3 days. (A) Immunofluorescent analysis of CD68+LYVE-1+ (yellow arrows) and CD68+LYVE-1- (red arrows) macrophage subsets migrating near islets. 200× magnification. Scale bars: 30 μm. (B) Quantitative analysis of CD68+LYVE-1+ and CD68+LYVE-1- cells surrounding islets. Each symbol represents one islet. 43–52 islets/group, 4–5 slides/mice, 3 mice/group. *** P≤0.001. Mean ± SD.

From: Yin N, Zhang N, Lal G, Xu J, Yan M, et al.
(2011) Lymphangiogenesis Is Required for Pancreatic Islet Inflammation and Diabetes.
PLoS ONE 6(11): e28023.

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Mouse anti CD68 antibody, clone FA-11 used for the detection of macrophages in mouse brain by immunohistochemistry on formain fixed, paraffin embedded tissue.
Image caption:
Iminosugar-based GCS inhibitors decrease the number of brain CD68 positive cells. (A) CD68 immunolabeling of brain stem from 28, 56, 84 and 112 day old Sandhoff mice, untreated or treated with either Genz-529468 or NB-DNJ. Dark brown cells are positive for CD68; scale bar = 50 μm. (B) Quantification of CD68+ cell counts in the brain stem, cerebellum, hippocampus and thalamus of 112 day old drug-treated Sandhoff mice (n = 4–5 mice per group). Cell counts are presented relative to those in untreated Sandhoff mice. Statistics are between untreated and treated Sandhoff mice, and were determined using the Graphpad Prism software t test; * = p<0.05. Error bars indicate SEM.

From: Ashe KM, Bangari D, Li L, Cabrera-Salazar MA, Bercury SD, et al. (2011)
Iminosugar-Based Inhibitors of Glucosylceramide Synthase Increase Brain Glycosphingolipids and Survival in a Mouse Model of Sandhoff Disease.
PLoS One. 2011;6(6):e21758.

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Mouse anti CD68 antibody, clone FA-11 used for the detection of macrophages in mouse atherosclerotic plaques by immunofluorescence.
Image caption:
Impact of SOCS-1 deficiency on atherosclerotic plaque composition A. Atherosclerotic plaques from Socs-1-/- triple-KO (Socs-1-/-) mice showed an increased CD68 (red);iNOS (green) double-positive cell content. B. CD68 (red); CD206 (green) double-positive cells were hardly detected. C. Atherosclerotic plaques from Socs-1-/-triple-KO (Socs-1-/-) mice contained slightly more MOMA-2 (red); Ly-6C (green) double-positive cells after 4 weeks of HCD as well as D. a higher number of Ly-6G positive cells (scale bar A–C: 20 μm, scale bar D:50 μm). N = 5–7 animals per group.

From: Grothusen C, Schuett H, Hillmer A, Lumpe S, Grote K, et al. (2012)
Role of Suppressor of Cytokine Signaling-1 In Murine Atherosclerosis.
PLoS ONE 7(12): e51608.

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Mouse anti CD68 antibody, clone FA-11 used for the detection of macrophages in mouse vacscular tissue by immunofluorescence.
Image caption:
CD68 positive macrophages/monocytes cells can be seen within early developing neointima in some mice. Sections from the same two mice shown in Figure 6 were stained with antibodies to CD68 (white). Left panels show mTomato (red)/mEGFP (green) co-stained sections and in the right panels are the same sections visualized for mEGFP (green) and CD68 (white). CD68-positive cells can be seen within the neointima of vessels from the mouse shown in the upper panels but not in the vessels of the mouse shown in the lower panels. Nuclei were visualized by staining with DAPI (blue). All images are shown at the same magnification with the scale bar shown in the bottom left panel representing 50 μm.

From: Herring BP, Hoggatt AM, Burlak C, Offermanns S.
Previously differentiated medial vascular smooth muscle cells contribute to neointima formation following vascular injury.
Vasc Cell. 2014 Oct 1;6:21.

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Mouse anti CD68 antibody, clone FA-11 used for the detection of macrophages in mouse lung and liver by immunohistochemistry on formalin fixed, paraffin embedded tissues.
Image caption:
Histology in 9V/9V/GCStg and 9V/null/GCStg mice. (A) The lung and liver sections from 9-wk old 9V/9V (row 1), 9V/9V/GCStg (row 2), 9V/null (row 3) and 9V/null/GCStg (row 4) were processed for H&E and CD68 antibody staining as indicated. Large and pale storage cells were observed in H&E stained lung and liver sections (arrows). The macrophages were indicated by anti-CD68 immunostaining (brown). Images were captured by Zeiss microscope with Spot Advance software. Scale bar was 40 μm for all images. (B) The distribution and density of macrophages in 9V/null/GCStg lung and liver immunostained by anti-CD68 antibody (brown). Scale bar was 40 μm for both images. (C) CD68 positive cells (CD68+) in 9V/9V/GCStg and 9V/null/GCStg lungs had significantly more CD68 stained macrophages than 9V/9V and 9V/null at 9 wks of age, respectively. The data present number of cells per image of total 5-15 images/mouse, 3 mice per genotype. Results with error bars are mean ≤S.E. The p values were from Student's t-test.

From: Barnes S, Xu Y-H, Zhang W, Liou B, Setchell KDR, et al. (2014)
Ubiquitous Transgene Expression of the Glucosylceramide-Synthesizing Enzyme Accelerates Glucosylceramide Accumulation and Storage Cells in a Gaucher Disease Mouse Model.
PLoS ONE 9(12): e116023.

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Rat anti Mouse CD68 antibody, clone FA-11 used for the detection of infiltrating macrophages in mouse kidney by immunohistochemistry on paraffin embedded material.
Image caption:
Reduction in cellular infiltration and inflammatory markers in UUO kidneys exposed to telmisartan or PXS64. Untreated UUO kidneys showed increased F4/80, CD68 and CD45 positively stained cells as compared to the sham operated control animals. PXS64 significantly reduced F4/80 and CD45 positive stained cells (Fig. 6C and E) with a trend to a reduction in CD68 cells, although this was not statistically significant (Fig. 6D). Telmisartan treated kidneys showed a reduction in F4/80 positive cells but no difference in CD45 or CD68 stained cells, suggesting a differential action of PXS64 and telmisartan in modifying cellular infiltration. Results are presented as mean showed (n = 8, *P < 0.05 vs. UUO, ** P < 0.01 vs. UUO). Magnification x 400.

From: Zhang J, Wong MG, Wong M, Gross S, Chen J, et al. (2015)
A Cationic-Independent Mannose 6-Phosphate Receptor Inhibitor (PXS64) Ameliorates Kidney Fibrosis by Inhibiting Activation of Transforming Growth Factor-β1. PLoS ONE 10(2): e0116888.

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Rat anti Mouse CD68 antibody, clone FA-11 used for the detection of macrophages in murine cervical lymph nodes by immunofluorescence.
Image caption:
Brucella and fluorescent microspheres in the CLN localize in cells positive for CD68 and low or negative for CD11c. (A) C57BL/6 mice were fed by oral gavage with 0.2 μm yellow green fluorescent microspheres. After 3 days, they were sacrificed and CLN processed for immunofluorescence microscopy. (B) Cells with internal beads from experiments as shown in (A) were quantified as to their expression of CD11c and CD68. At least 100 bead-containing cells per experiment were counted. (C) Mice infected by the oral route with 109 B. melitensis per mouse were sacrificed at day 8, CLN prepared for immunofluorescence analysis as described above and (D) the number of infected cells positive for either marker was determined. All available cuts from the CLN of one mouse were analyzed. Data shown represents mean and standard deviation of three independent experiments. Bars: 10 μm.

From: von Bargen K, Gagnaire A, Arce-Gorvel V, de Bovis B, Baudimont F, Chasson L, et al. (2015)
Cervical Lymph Nodes as a Selective Niche for Brucella during Oral Infections.
PLoS ONE 10(4): e0121790.

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Rat anti Mouse CD68 antibody, clone FA-11 used for the identification of macrophages in atherosclerotic lesions by immunofluorescence.
Image caption:
Macrophage and VSMC accumulation is reduced in uPAR-/-/LDLR-/-. (A) Representative Mac-3 immunostaining and (B) %-Mac-3-positive lesion per lesion area. (C) Representative anti-SMC immunostaining and (D) % anti-SMC-positive lesion per lesion area. (E) PCNA/CD68 double staining excludes differences in macrophage proliferation in the lesions. (F) Macrophage apoptosis was not responsible for the differences in macrophage content as evidenced by cleaved caspase 3 staining. Mean±SEM, n = 8. *P<0.05, **P<0.01, ***P<0.001. A/C: Bar = 250 μm, E/F Bar = 100μm αSMA: alpha smooth-muscle-actin, PCNA: Proliferating-Cell-Nuclear-Antigen, cC3: cleaved caspase-3, DAPI: 4',6-Diamidin-2-phenylindol.

From:Larmann J, Jurk K, Janssen H, Müller M, Herzog C, Lorenz A, et al. (2015)
Hepatic Overexpression of Soluble Urokinase Receptor (uPAR) Suppresses Diet-Induced Atherosclerosis in Low-Density Lipoprotein Receptor-Deficient (LDLR-/-) Mice.
PLoS ONE 10(8): e0131854.

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Rat anti Mouse CD68 antibody, clone FA-11 used for the identification of macrophages in atherosclerotic lesions by immunohistochemistry on cryosections.
Image caption:
Guide-wire injury induced intimal hyperplasia. Mice were subjected to guide wire injury of the internal carotid artery after hypercholesterolemia had been induced. (A) Representative Micrographs of carotid artery lesions in the injured and the contralateral artery. GWI induced concentric lesions did not differ in (B) size or (C) lipid content. (D) Macrophage recruitment did occur, but (E) differences between uPAR-deficient and uPAR-wild-type lesions were not observed in this model. Additionally, no differences between uPAR+/+ and uPAR-/- animals were observable with respect to (F-G) VSMC content of the lesions. No relevant lesion formation was observed in the contralateral sham vessels. (H) Proliferating cells in atherosclerotic lesions are mainly non-VSMC. The amount of proliferating VSMC appears similar for the two genotypes. A, D, F: Bar = 250μm, H: Bar: 50μm, mean±SEM, n = 8. n.s.: not significant, αSMA: alpha smooth-muscle-actin, DAPI: 4',6-Diamidin-2-phenylindol, GWI: guide-wire injury, L: left, R: right, PCNA: Proliferating Cell Nuclear Antigen.

From:Larmann J, Jurk K, Janssen H, Müller M, Herzog C, Lorenz A, et al. (2015)
Hepatic Overexpression of Soluble Urokinase Receptor (uPAR) Suppresses Diet-Induced Atherosclerosis in Low-Density Lipoprotein Receptor-Deficient (LDLR-/-) Mice.
PLoS ONE 10(8): e0131854.

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Rat anti Mouse CD68 antibody, clone FA-11 used for the identification of macrophages in atherosclerotic lesions by immunofluorescence.
Image caption:
Hepatic overexpression of soluble full-length uPAR inhibits atherosclerotic lesion development and macrophage accumulation in LDLR-/- mice. A, Immunostaining for the c-myc-tag revealed expression of soluble uPAR in the livers of mice after hydrodynamic transfection. Bar = 100 μm B. Oil red-O staining of aortic valve cryosections. Bar = 250 μm C. Atherosclerotic lesion size was assessed as % total aortic sinus lumen area occupied. D, Representative CD68 immunostaining and E. % CD68-positive lesion area. F, Representative anti-αSMC immunostaining and G. % anti-αSMA-positive lesion area. Bar = 250 μm, Mean±SEM, n = 6/8. *P<0.05, **P<0.01, ***P<0.001. aSMA: alpha smooth-muscle-actin, DAPI: 4',6-Diamidin-2-phenylindol

From:Larmann J, Jurk K, Janssen H, Müller M, Herzog C, Lorenz A, et al. (2015)
Hepatic Overexpression of Soluble Urokinase Receptor (uPAR) Suppresses Diet-Induced Atherosclerosis in Low-Density Lipoprotein Receptor-Deficient (LDLR-/-) Mice.
PLoS ONE 10(8): e0131854.

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Rat anti Mouse CD68 antibody, clone FA-11 (MCA1957GA) used for the detection of macrophages in murine kidney using immunohistochemistry on PLP fixed cryosections.
Image caption:
Representative histological sections from the 3 bone marrow transplant groups with NTN. (A) PAS-stained sections of renal histology. The WT to IL-17-/- group have severe glomerular thrombosis with profound tubular dilatation and casts, significantly more than the other groups. (B) and (C) Immunofluorescence for glomerular deposition of sheep and mouse IgG respectively. There was no difference between the groups. (D) and (E) Representative glomerular staining for CD68 and CD4 respectively. Magnification x400.

From: Hamour S, Gan P-Y, Pepper R, Florez Barros F, Wang H-H, O'Sullivan K, et al. (2015)
Local IL-17 Production Exerts a Protective Role in Murine Experimental Glomerulonephritis.
PLoS ONE 10(8): e0136238.

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Biotin conjugated Rat anti Mouse CD68antibody, clone FA-11 used for the assessment of CD68 expression on Kuppfer cells by flow cytometry.
Image caption:
The flow cytometric analysis of liver F4/80+CD11b+ cells and F4/80+CD68+ cells three days after Sham operated or PHx mice
(A). The changes in the proportions of F4/80+CD11b+ cells in the remnant livers (B). The changes in the proportions of F4/80+CD68+ cells in the remnant livers (C). The percentages of F4/80+CD11b+ cells which are indicated by red dots and F4/80+CD68+ cells indicated by blue dots are the representative data from four to six mice (A). The percentages of each cellular population at the indicated time points are shown as the means±SE (B, C). (*P < .05 vs Sham).

From: Nishiyama K, Nakashima H, Ikarashi M, Kinoshita M, Nakashima M, Aosasa S, et al. (2015)
Mouse CD11b+Kupffer Cells Recruited from Bone Marrow Accelerate Liver Regeneration after Partial Hepatectomy.
PLoS ONE 10(9): e0136774.

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Rat anti Mouse CD68 antibody, clone FA-11 used for the identification of inflammatory macrophagesat the site of tibial fractire by immunofluorescence
Image caption:
Bone fracture increased CD68+ macrophages in the peri-infarct region. (A) Image illustrates infarct core (Core), infarct border (dotted line) and the peri-infarct region (P.I.). (B) Quantification of CD68+ cells. *: P = 0.004, compared to stroke-only group. (C) Representative images of anti-CD68 antibody-stained sections. BF + 6hS: mice that received tibia fracture 6 hours before pMCAO; BF+1dS: mice that received tibia fracture 24 hours before pMCAO. Scale bars: 50μm. N = 6.

From: Wang L, Kang S, Zou D, Zhan L, Li Z, Zhu W, et al. (2016)
Bone Fracture Pre-Ischemic Stroke Exacerbates Ischemic Cerebral Injury in Mice.
PLoS ONE 11(4): e0153835.

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CD68 Antibody | FA-11 gallery image 33

Published customer image:
Rat anti Mouse CD68 antibody, clone FA11 used for the identification of macrophages and microglia in unfixed cryosections by immunofluorescence.
Image caption:
Engulfment of fibrillar Aβ by BMDM and primary microglia AA schematic shows 10-µm cryosections of unfixed brain from 6-month-old APP/PS1 mice, which show a high amyloid plaque burden (right panel with methoxy-X04 staining), were incubated with antibody for 1 h, followed by adding BMDM or primary microglia on top of the sections. After 24 h incubation, sections were analyzed by immunostaining or immunoblotting.BmAb11 but not the isotype control (IC) co-localized with methoxy-X04. Scale bar: 10 μm.C, DBMDM (C) or primary microglia (D) were cultured on cryosections pre-incubated with mAb11 (1 μg/ml). After 24 h, sections were processed for immunostaining using antibody against CD68 to identify myeloid cells and methoxy-X04 staining to visualize AΒ. Note that both cell types internalize Aβ into intracellular vesicles (right panels show enlargement of insets). Scale bar: 10 μm.

From: Xiang X, Werner G, Bohrmann B, Liesz A, Mazaheri F, Capell A, Feederle R, Knuesel I, Kleinberger G, Haass C.
TREM2 deficiency reduces the efficacy of immunotherapeutic amyloid clearance.
EMBO Mol Med. 2016 Sep 1;8(9):992-1004.

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CD68 Antibody | FA-11 gallery image 34

Published customer image:
Rat anti Mouse CD68 antibody, clone FA11 (MCA1957) used for the identification of macrophages in post stroke infarcts by immunohistochemistry on frozen tissue sections.
Image caption:
Immune cell composition in infarcts at the stage of liquefactive necrosis in humans and mice. a Representative images of CD3+ T-lymphocyte, CD20+ B-lymphocyte, and CD68+ macrophage/microglia infiltration in human infarcts at the stage of liquefactive necrosis. Scale bars, 10 μm (human, CD3 and CD20 images) and 30 μm (human, CD68 image). b Quantification of CD4+ and CD8+ T-lymphocyte, and CD20+ B-lymphocyte infiltration into the infarcts. c Representative images of CD3+ T-lymphocytes, B220+ B-lymphocytes, and CD68+ macrophages/microglia in the infarcts of C57BL/6 and BALB/c mice at 7 weeks post-stroke. Scale bar,100 μm. d Higher magnification images of CD68+ macrophages/microglia in the infarct to reveal individual cells. Scale bar, 25 μm. e Quantification of CD3+ T-lymphocyte infiltration, B220+ B-lymphocyte infiltration, and CD68 immunostaining in mouse infarcts. ****p?
From: Nguyen TV, Frye JB, Zbesko JC, Stepanovic K, Hayes M, Urzua A, Serrano G, Beach TG, Doyle KP.Acta Neuropathol Commun. 2016 Sep 6;4(1):100.

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CD68 Antibody | FA-11 gallery image 35

Published customer image:
Rat anti Mouse CD68 antibody, clone FA11 (MCA1957) used for the identification of macrophages in post stroke infarcts by immunohistochemistry on frozen tissue sections.
Image caption:
Impact of age on the chronic inflammatory response to stroke in C57BL/6 mice. a Comparison of the levels of 25 cytokines and chemokines in infarcts at the stage of liquefactive necrosis dissected from 3-month old and 18-month old C57BL/6 mice at 7 weeks post-stroke. Data are expressed as a fold-change relative to age matched sham control values. Data represent mean?±?SEM. There is no significant difference in the overall cytokine and chemokine profile at the stage of liquefactive necrosis between 3-month old and 18-month old mice by two-way ANOVA. Cytokines and chemokines that are significantly different between the 3-month old and 18-month old mice corrected for multiple comparisons are denoted by an asterisk (*p?<?0.05 versus 3-month old mice). b Representative images of CD3+ T-lymphocytes, B220+ B-lymphocytes, and CD68+ macrophages/microglia in the infarcts of 3-month old and 18-month old C57BL/6 mice at 7 weeks post-stroke. Scale bar, 50 μm. Red dotted lines indicate locations of the glial scar in each image. c Quantification of CD3+ T-lymphocyte and B220+ B-lymphocyte infiltration into the mouse infarcts.

From: Nguyen TV, Frye JB, Zbesko JC, Stepanovic K, Hayes M, Urzua A, Serrano G, Beach TG, Doyle KP.Acta Neuropathol Commun. 2016 Sep 6;4(1):100.

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CD68 Antibody | FA-11 gallery image 36

Published customer image:
Alexaflour™488 conjugated Rat anti Mouse CD68 antibody, clone FA-11 (MCA1957A488) used for visualisation of CD68 expressing cells in brain lesions by immunofluorescence and purified Rat anti Mouse CD68 antibody, clone FA-11 (MCA1957) used to assess CD68 expression in similar lesions by western blotting.
Image caption:
Analysis of M/M phenotypic markers at different time points after dMCAO. a Coronal sections of the lesioned hemispheres of the inhibitor and control mice brains, at seven (two upper rows of panels) and 14 days (two lower rows) after dMCAO. The sections were triple-immunostained with anti-Iba-1 (red), anti-CD45 (blue), and anti-CD68 (green) antibodies. Representative merged and single-channel images depict part of the infarct core (I, outlined with white dotted lines) and peri-infarct area of stroke. High magnification images demonstrate distribution of Iba-1, CD45, and CD68 in the glial scar area (right panels). Imaging was performed using Zeiss LSM800 Airyscan confocal microscope, using single-scan and tile-scan image acquisitions. Bars: from left to right: 100 and 10 μm. b Quantification of the corrected fluorescence intensity for CD45 and CD68 immunofluorescence staining in the inhibitor (black bars) and control groups (white bar), at 7 and 14 days after dMCAO. N?=?5–6 mice per group/per time point, three brain sections per mouse. Error bars: SEM; Student's t test, *p<0.05; **p<0.01. c Representative Western blots and quantification analysis (graphs) of CD206, CD45, CD68, and Iba-1 expression in lysates prepared from the lesioned cortical tissue of the animals from the inhibitor (black bars) and control (white bars) groups, at 7 and 14 days after dMCAO. Graphs: optical density of each band was normalized to β-actin (loading control). N?=?5–6 mice per group/per time point. Error bars: SEM; Student's t test, *p<0.05

From: Pena-Philippides JC, Caballero-Garrido E, Lordkipanidze T, Roitbak T.
In vivo inhibition of miR-155 significantly alters post-stroke inflammatory response.
J Neuroinflammation. 2016 Nov 9;13(1):287.

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CD68 Antibody | FA-11 gallery image 37

Published customer image:
Rat anti Mouse CD68 antibody, clone FA-11 used for the identification of infiltrating microglia in a murine model of Retinitis pigmentosa by immunofluorescence on vibrotome sections.
Image caption:
Transient expression of phagocytic molecules and exposure of the “eat-me” signal phosphatidylserine (PS) in the outer nuclear layer during rod degeneration
A–C Microglia infiltrating the ONL demonstrate upregulation of the phagocytic molecule, CD68. Top images in (A) show composites of CD68 (red), Iba1 (green), and DAPI (blue) staining; bottom images show the same field with CD68 staining only. At P18, non-infiltrating microglia expressed low or undetectable levels of CD68, a lysosome-associated membrane protein. At P21–23, microglia infiltrating the ONL strongly upregulated CD68 (arrowheads). At P30, CD68 immunopositivity in ONL microglia decreased and was mostly confined to amoeboid cells in the subretinal space. (B) Magnified view of inset in (A) demonstrates localization of CD68 to microglia phagosomes. (C) CD68 expression (area of immunopositivity as a fraction of the ONL) demonstrated a peaked at P22 and decreased by P30. Scale bar, 20 μm.
D–F Upregulation of MFG-E8, a secreted glycoprotein that serves as a bridging molecule for phagocytosis receptors, in the ONL. (D) MFG-E8 was low or absent in the ONL at P18, but emerged at P21–23, localizing to photoreceptor cell bodies in a column-like pattern (arrow) and as a punctate signal within infiltrating microglia (arrowhead), before decreasing throughout the ONL at P30. (E) A magnified orthogonal view of the inset from (D) demonstrating punctate MFG-E8 immunopositivity within ONL microglia. (F) Quantification of MFG-E8 expression demonstrated a prominent emergence at P22 and a subsequent decrement by P30. Scale bar, 20 μm.
G–K Increase in phosphatidylserine (PS) exposure in the ONL during photoreceptor degeneration. (G) At P18, PS immunopositivity is near absent in the ONL, but increased significantly in ONL somata at P21–23, before decreasing at P30. (H) Quantitation of PS exposure by image analysis (by fractional area of PS immunopositivity within the ONL (top), and the mean intensity of PS staining in the ONL (bottom)) demonstrated a transient increase at P22. (I) Co-immunolabeling of rods with rhodopsin at P22 demonstrates that PS exposure was present in a majority of rods (inset shows at high magnification the co-labeling of PS and rhodopsin in multiple rod somata). (J) Conversely, immunolabeling of cones with cone arrestin demonstrates the sparse co-localization of PS in cones (inset shows close juxtaposition but no colocalization of PS and arrestin labeling). (K) Scoring of rhodopsin+ rods and arrestin+ cones for PS co-labeling demonstrates that a large majority of rods, but only a small minority of cones, showed PS exposure (two-sided unpaired t-test, n = 3 animals at P22). Scale bars = 20 μm.
Data information: Quantitative analyses in (C, F, H, and K) involved three animals at each time point. Column heights and error bars indicate mean ± SEM.

From: Zhao L, Zabel MK, Wang X, Ma W, Shah P, Fariss RN, Qian H, Parkhurst CN, Gan WB, Wong WT.
Microglial phagocytosis of living photoreceptors contributes to inherited retinal degeneration.
EMBO Mol Med. 2015 Jul 2;7(9):1179-97.

Enlarge
CD68 Antibody | FA-11 gallery image 38

Published customer image:
Rat anti Mouse CD68 antibody, clone FA-11 (MCA1957GA) used for the identification of microglia engulfing rods in the retinal outer nuclear layer (ONL) in a murine model of retinitis pigmentoisa by immunofluorescence.
Image caption:
Infiltrating microglia phagocytose non-apoptotic photoreceptor rods during rod degeneration
A–C Phagocytosis of rods by infiltrating microglia. (A) Representative example of a Iba1+ microglial process extending into the ONL with a phagosome at its terminal end. Each phagosome contained a photoreceptor nucleus (labeled with DAPI, arrow) that was identified as a rod photoreceptor by rhodopsin immunopositivity (superposition of Iba1+ phagosome with rhodopsin+ soma in orthogonal views). (B) Example of an amoeboid microglia in the ONL with multiple phagosomes containing both rhodopsin-positive (arrowhead) and rhodopsin-negative (arrow) nuclei. (C) Rhodopsin+ nuclei can be localized within CD68-positive phagosomes in infiltrating microglia, indicating phagocytic engulfment of rods. Scale bar, 10 μm.

From: Zhao L, Zabel MK, Wang X, Ma W, Shah P, Fariss RN, Qian H, Parkhurst CN, Gan WB, Wong WT.
Microglial phagocytosis of living photoreceptors contributes to inherited retinal degeneration.
EMBO Mol Med. 2015 Jul 2;7(9):1179-97.

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  • Rat anti Mouse CD68:Endotoxin Low
  • Rat anti Mouse CD68:Biotin
  • Rat anti Mouse CD68
  • Rat anti Mouse CD68:FITC
  • Rat anti Mouse CD68:Alexa Fluor® 488
  • Rat anti Mouse CD68:Alexa Fluor® 488
  • Rat anti Mouse CD68:Alexa Fluor® 700
  • Rat anti Mouse CD68:Alexa Fluor® 647
  • Rat anti Mouse CD68:FITC
  • Rat anti Mouse CD68:RPE
  • Rat anti Mouse CD68:Alexa Fluor® 700
  • Rat anti Mouse CD68:RPE
  • Rat anti Mouse CD68
  • Rat anti Mouse CD68:Biotin
  • Rat anti Mouse CD68
  • Rat anti Mouse CD68:Alexa Fluor® 647
  • Rat anti Mouse CD68:FITC
(Rated 5.0 out of 5 based on 1 customer reviews)
    CD68 antibody, clone FA-11 recognizes mouse macrosialin which is a homolog of human CD68, which is classified as a unique scavenger receptor (ScR) family member. CD68 is considered a pan macrophage marker expressed on the intracellular lysosomes of tissue macrophages.
    CD68 price reduction stamp
    • Product Type
      Monoclonal Antibody
    • Clone
      FA-11
    • Isotype
      IgG2a
    8 Formats Available
      Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
      MCA1957BCdatasheet pdfdatasheet pdf0.1 mg
      MCA1957B
      MCA1957GAC, F *, IF, IP, P *, WB*datasheet pdfdatasheet pdf0.1 mg
      MCA1957GA
      MCA1957FF *datasheet pdfdatasheet pdf0.1 mg
      MCA1957F
      MCA1957C, F *, IF, IP, P *, WB*datasheet pdfdatasheet pdf0.25 mg
      MCA1957
      MCA1957ELC, F *, IF, IP, P *, WB*datasheet pdfdatasheet pdf0.5 mg
      MCA1957EL
      MCA1957PEF *datasheet pdfdatasheet pdf100 Tests
      MCA1957PE
      MCA1957A488F *datasheet pdfdatasheet pdf100 Tests/1ml
      MCA1957A488
      MCA1957A700F *datasheet pdfdatasheet pdf100 Tests/1ml
      MCA1957A700
      MCA1957A647F *datasheet pdfdatasheet pdf100 Tests/1ml
      MCA1957A647
      MCA1957FTF *datasheet pdfdatasheet pdf25 µg
      MCA1957FT
      MCA1957TC, F *, IF, IP, P *, WB*datasheet pdfdatasheet pdf25 µg
      MCA1957T
      MCA1957BTCdatasheet pdfdatasheet pdf25 µg
      MCA1957BT
      MCA1957PETF *datasheet pdfdatasheet pdf25 Tests
      MCA1957PET
      MCA1957A488TF *datasheet pdfdatasheet pdf25 Tests/0.25ml
      MCA1957A488T
      MCA1957A647TF *datasheet pdfdatasheet pdf25 Tests/0.25ml
      MCA1957A647T
      MCA1957A700TF *datasheet pdfdatasheet pdf25 Tests/0.25ml
      MCA1957A700T
      MCA1957FAF *datasheet pdfdatasheet pdf50 µg
      MCA1957FA
      Summary
      Secondary Antibodies
      Negative Isotype Controls
      Useful Reagents
      Positive Controls
      Histology Controls
      More Images
      References
      Reviews
      -
      • Rat anti mouse CD68 antibody, clone FA-11 recognizes mouse macrosialin, a heavily glycosylated transmembrane protein and murine homolog of human CD68, which is classified as a unique scavenger receptor (ScR) family member, due to the presence of a lysosome associated membrane protein (LAMP)-like domain.

        CD68 is considered a pan macrophage marker, predominantly expressed on the intracellular lysosomes of tissue macrophages/monocytes, including Kupffer cells, microglia, histiocytes and osteoclasts, and is expressed to a lesser extent by dendritic cells and peripheral blood granulocytes.

        CD68 is expressed by many tumor types including some B cell lymphomas, blastic NK lymphomas, melanomas, granulocytic (myeloid) sarcomas, hairy cell leukemias, and renal, urinary and pancreatic tumors, and can be used in cancer studies to demonstrate the presence/localization of macrophages.

        Rat anti mouse CD68 antibody, clone FA-11, has been used in many mouse models for the identification of CD68 in immunohistochemical studies, using both frozen and paraffin-embedded tissues ( Masaki et al. 2003) and Devey et al. 2009).

        Rat anti mouse CD68 antibody, clone FA-11 can be used in flow cytometry to detect intracellular CD68, following permeabilization, and can detect surface macrosialin at low levels in resident mouse peritoneal macrophages which can be enhanced with thioglycollate stimulation.
      • Intended Use
      • Target Species
        Mouse
      • Product Form
        Purified IgG - liquid
        Purified IgG conjugated to Biotin - liquid
        Purified IgG - liquid
        Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
        Purified IgG conjugated to Alexa Fluor® 488 - liquid
        Purified IgG conjugated to Alexa Fluor® 488 - liquid
        Purified IgG conjugated to Alexa Fluor® 700 - liquid
        Purified IgG conjugated to Alexa Fluor® 647 - liquid
        Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
        Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilised
        Purified IgG conjugated to Alexa Fluor® 700 - liquid
        Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilised
        Purified IgG - liquid
        Purified IgG conjugated to Biotin - liquid
        Purified IgG - liquid
        Purified IgG conjugated to Alexa Fluor® 647 - liquid
        Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
      • Reconstitution
        Pack Size: 25 TestsReconstitute in 0.25 ml disilled water
        Pack Size: 100 TestsReconstitute with 1.0 ml distilled water
        Pack Size: 25 TestsReconstitute in 0.25 ml disilled water
        Pack Size: 100 TestsReconstitute with 1.0 ml distilled water
      • Preparation
        Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
        Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
        Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
        Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
        Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
        Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
        Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
        Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
        Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
        Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
        Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
        Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
        Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
        Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
        Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
        Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
        Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      • Preservative Stabilisers
        None present
        0.09%Sodium Azide
        1%Bovine Serum Albumin
        0.09%Sodium Azide
        0.09%Sodium Azide
        1%Bovine Serum Albumin
        0.09%Sodium Azide
        1%Bovine Serum Albumin
        0.09%Sodium Azide
        1%Bovine Serum Albumin
        0.09%Sodium Azide
        1%Bovine Serum Albumin
        0.09%Sodium Azide
        1%Bovine Serum Albumin
        0.09%Sodium Azide
        1%Bovine Serum Albumin
        0.09%Sodium Azide
        1%Bovine Serum Albumin
        5%Sucrose
        0.09%Sodium Azide
        1%Bovine Serum Albumin
        0.09%Sodium Azide
        1%Bovine Serum Albumin
        5%Sucrose
        0.09%Sodium Azide
        0.09%Sodium Azide
        1%Bovine Serum Albumin
        0.09%Sodium Azide
        0.09%Sodium Azide
        1%Bovine Serum Albumin
        0.09%Sodium Azide
        1%Bovine Serum Albumin
      • Immunogen
        Purified Concanavalin A acceptor glycoprotein from P815 cell line.
      • Purity
      • Approx. Protein Concentrations
        IgG concentration 1.0mg/ml
        IgG concentration 0.1mg/ml
        IgG concentration 1.0mg/ml
        IgG concentration 0.1mg/ml
        IgG concentration 0.05 mg/ml
        IgG concentration 0.05 mg/ml
        IgG concentration 0.05 mg/ml
        IgG concentration 0.05 mg/ml
        IgG concentration 0.1mg/ml
        IgG concentration 0.05 mg/ml
        IgG concentration 1.0mg/ml
        IgG concentration 0.1mg/ml
        IgG concentration 1.0mg/ml
        IgG concentration 0.05 mg/ml
        IgG concentration 0.1mg/ml
      • Reagents In The Kit
      • Preparing The Antibody
      • Test Principle
      • Buffer Solution
        Phosphate buffered saline
        Phosphate buffered saline
        Phosphate buffered saline
        Phosphate buffered saline
        Phosphate buffered saline
        Phosphate buffered saline
        Phosphate buffered saline
        Phosphate buffered saline
        Phosphate buffered saline
        Phosphate buffered saline
        Phosphate buffered saline
        Phosphate buffered saline
        Phosphate buffered saline
        Phosphate buffered saline
        Phosphate buffered saline
        Phosphate buffered saline
        Phosphate buffered saline
      • Storage
        Store at -20oC only.

        This product should be stored undiluted.

        Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
        Store at +4oC or at -20oC if preferred.

        This product should be stored undiluted.

        Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
        Store at +4oC or at -20oC if preferred.

        This product should be stored undiluted.

        Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
        Store at +4oC or at -20oC if preferred.

        This product should be stored undiluted.

        Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.

        Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
        Store at +4oC or at -20oC if preferred.

        This product should be stored undiluted.

        Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.

        Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
        Store at +4oC or at -20oC if preferred.

        This product should be stored undiluted.

        Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.

        Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
        Store at +4oC or at -20oC if preferred.

        This product should be stored undiluted.

        Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.

        Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
        Store at +4oC or at -20oC if preferred.

        This product should be stored undiluted.

        Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.

        Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
        Store at +4oC or at -20oC if preferred.

        This product should be stored undiluted.

        Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.

        Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
        Store at +4oC.

        DO NOT FREEZE.

        This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
        Store at +4oC or at -20oC if preferred.

        This product should be stored undiluted.

        Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.

        Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
        Store at +4oC.

        DO NOT FREEZE.

        This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
        Store at +4oC or at -20oC if preferred.

        This product should be stored undiluted.

        Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
        Store at +4oC or at -20oC if preferred.

        This product should be stored undiluted.

        Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
        Store at +4oC or at -20oC if preferred.

        This product should be stored undiluted.

        Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
        Store at +4oC or at -20oC if preferred.

        This product should be stored undiluted.

        Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.

        Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
        Store at +4oC or at -20oC if preferred.

        This product should be stored undiluted.

        Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.

        Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      • Shelf Life
        18 months from date of despatch.
        18 months from date of despatch.
        18 months from date of despatch.
        18 months from date of despatch.
        18 months from date of despatch.
        18 months from date of despatch.
        18 months from date of despatch.
        18 months from date of despatch.
        18 months from date of despatch.
        12 months from date of reconstitution.
        18 months from date of despatch.
        12 months from date of reconstitution.
        18 months from date of despatch.
        18 months from date of despatch.
        18 months from date of despatch.
        18 months from date of despatch.
        18 months from date of despatch.
      • GO Terms
        lysosomal membrane
        integral to membrane
        plasma membrane
        endosome membrane
      • UniProt
      • Entrez Gene
      • Acknowledgements
        This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
        This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
        This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
        This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
        This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
        This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
      • Regulatory
        For research purposes only
      • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

      • Application NameYesNoMin DilutionMax Dilution
        Immunofluorescence
        Immunohistology - Frozen
        Immunoprecipitation
        Flow Cytometry(1)1/501/100
        Immunohistology - Paraffin(2)
        Western Blotting(3)
        (1)
        Membrane permeabilisation is required for this application. Bio-Rad recommends the use of Leucoperm™ (Product Code BUF09) for this purpose.
        (2)
        This product may require antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose. See Martin-Manso for details. Staining has also been achieved without antigen retrieval, see Lu for details.
        (3)
        Non-reducing conditions recommended.
      • Application NameYesNoMin DilutionMax Dilution
        Immunohistology - Frozen1/10
      • Application NameYesNoMin DilutionMax Dilution
        Immunofluorescence
        Immunohistology - Frozen
        Immunoprecipitation
        Flow Cytometry(1)1/501/100
        Immunohistology - Paraffin(2)
        Western Blotting(3)
        (1)
        Membrane permeabilisation is required for this application. Bio-Rad recommends the use of Leucoperm™ (Product Code BUF09) for this purpose.
        (2)
        This product may require antigen retrieval using heat treatment prior to staining of paraffin sections. Either sodium citrate buffer or Tris/EDTA buffer may be used for this purpose. See Martin-Manso for details. Staining has also been achieved without antigen retrieval, see Lu for details.
        (3)
        Non-reducing conditions recommended.
      • Application NameYesNoMin DilutionMax Dilution
        Flow Cytometry(1)Neat1/10
        (1)
        Membrane permeabilisation is required for this application. Bio-Rad recommends the use of Leucoperm™ (Product Code BUF09) for this purpose.
        The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors. This may be reduced by using SeroBlock FcR (Product Code BUF041).
      • Application NameYesNoMin DilutionMax Dilution
        Flow Cytometry(1)Neat1/10
        (1)
        Membrane permeabilisation is required for this application. Bio-Rad recommends the use of Leucoperm™ (Product Code BUF09) for this purpose.
        The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors. This may be reduced by using SeroBlock FcR (Product Code BUF041)
      • Application NameYesNoMin DilutionMax Dilution
        Flow Cytometry(1)Neat1/10
        (1)
        Membrane permeabilisation is required for this application. Bio-Rad recommends the use of Leucoperm™ (Product Code BUF09) for this purpose.
        The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors. This may be reduced by using SeroBlock FcR (Product Code BUF041)
      • Application NameYesNoMin DilutionMax Dilution
        Flow Cytometry(1)Neat1/10
        (1)
        Membrane permeabilisation is required for this application. Bio-Rad recommends the use of Leucoperm™ (Product Code BUF09) for this purpose.
        The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors. This may be reduced by using SeroBlock FcR (Product Code BUF041).
      • Application NameYesNoMin DilutionMax Dilution
        Flow Cytometry(1)Neat1/10
        (1)
        Membrane permeabilisation is required for this application. Bio-Rad recommends the use of Leucoperm™ (Product Code BUF09) for this purpose.
        The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors. This may be reduced by using SeroBlock FcR (Product Code BUF041A)
      • Application NameYesNoMin DilutionMax Dilution
        Flow Cytometry(1)Neat1/10
        (1)
        Membrane permeabilisation is required for this application. Bio-Rad recommends the use of Leucoperm™ (Product Code BUF09) for this purpose.
        The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors. This may be reduced by using SeroBlock FcR (Product Code BUF041).
      • Application NameYesNoMin DilutionMax Dilution
        Flow Cytometry(1)Neat
        (1)
        Membrane permeabilisation is required for this application. Bio-Rad recommends the use of Leucoperm™ (Product Code BUF09) for this purpose.
        The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors. This may be reduced by using SeroBlock FcR (Product Code BUF041).
      • Application NameYesNoMin DilutionMax Dilution
        Flow Cytometry(1)Neat1/10
        (1)
        Membrane permeabilisation is required for this application. Bio-Rad recommends the use of Leucoperm™ (Product Code BUF09) for this purpose.
        The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors. This may be reduced by using SeroBlock FcR (Product Code BUF041).
      • Application NameYesNoMin DilutionMax Dilution
        Flow Cytometry(1)Neat
        (1)
        Membrane permeabilisation is required for this application. Bio-Rad recommends the use of Leucoperm™ (Product Code BUF09) for this purpose.
        The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors. This may be reduced by using SeroBlock FcR (Product Code BUF041).
      • Application NameYesNoMin DilutionMax Dilution
        Immunofluorescence
        Immunohistology - Frozen
        Immunoprecipitation
        Flow Cytometry(1)1/501/100
        Immunohistology - Paraffin(2)
        Western Blotting(3)
        (1)
        Membrane permeabilisation is required for this application. Bio-Rad recommends the use of Leucoperm™ (Product Code BUF09) for this purpose.
        (2)
        This product may require antigen retrieval using heat treatment prior to staining of paraffin sections. Either sodium citrate buffer or Tris/EDTA buffer may be used for this purpose. See Martin-Manso for details. Staining has also been achieved without antigen retrieval, see Lu for details.
        (3)
        Non-reducing conditions recommended.
      • Application NameYesNoMin DilutionMax Dilution
        Immunohistology - Frozen1/10
      • Application NameYesNoMin DilutionMax Dilution
        Immunofluorescence
        Immunohistology - Frozen
        Immunoprecipitation
        Flow Cytometry(1)1/501/100
        Immunohistology - Paraffin(2)
        Western Blotting(3)
        (1)
        Membrane permeabilisation is required for this application. Bio-Rad recommends the use of Leucoperm™ (Product Code BUF09) for this purpose.
        (2)
        This product may require antigen retrieval using heat treatment prior to staining of paraffin sections. Either sodium citrate buffer or Tris/EDTA buffer may be used for this purpose. See Martin-Manso for details. Staining has also been achieved without antigen retrieval, see Lu for details.
        (3)
        Non-reducing conditions recommended.
      • Application NameYesNoMin DilutionMax Dilution
        Flow Cytometry(1)Neat1/10
        (1)
        Membrane permeabilisation is required for this application. Bio-Rad recommends the use of Leucoperm™ (Product Code BUF09) for this purpose.
        The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors. This may be reduced by using SeroBlock FcR (Product Code BUF041A)
      • Application NameYesNoMin DilutionMax Dilution
        Flow Cytometry(1)Neat1/10
        (1)
        Membrane permeabilisation is required for this application. Bio-Rad recommends the use of Leucoperm™ (Product Code BUF09) for this purpose.
        The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors. This may be reduced by using SeroBlock FcR (Product Code BUF041).

      • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
      • Technical Advice
      • Technical Advice
      • Technical Advice
      • Technical Advice
      • Technical Advice
      • Technical Advice
      • Technical Advice
      • Technical Advice
      • Technical Advice
      • Technical Advice
      • Technical Advice
      • Technical Advice
      • Technical Advice
      • Technical Advice
      • Technical Advice
      • Technical Advice
      • Technical Advice
      • Recommended Protocol
      • Recommended Protocol
      • Recommended Protocol
      • Recommended Protocol
      • Recommended Protocol
      • Recommended Protocol
      • Recommended Protocol
      • Recommended Protocol
      • Recommended Protocol
      • Recommended Protocol
      • Recommended Protocol
      • Recommended Protocol
      • Recommended Protocol
      • Recommended Protocol
      • Recommended Protocol
      • Recommended Protocol
      • Recommended Protocol
      • Flow Cytometry
        Use 10ul of the suggested working dilution to label 106 cells in 100ul. Recommended protocols are available Here
      • ELISA
      • ELISA
      • ELISA
      • ELISA
      • ELISA
      • ELISA
      • ELISA
      • ELISA
      • ELISA
      • ELISA
      • ELISA
      • ELISA
      • ELISA
      • ELISA
      • ELISA
      • ELISA
      • ELISA
      • Immunohistology
      • Immunohistology
      • Immunohistology
      • Immunohistology
      • Immunohistology
      • Immunohistology
      • Immunohistology
      • Immunohistology
      • Immunohistology
      • Immunohistology
      • Immunohistology
      • Immunohistology
      • Immunohistology
      • Immunohistology
      • Immunohistology
      • Immunohistology
      • Immunohistology
      • Histology Positive Control Tissue
      • Histology Positive Control Tissue
      • Histology Positive Control Tissue
      • Histology Positive Control Tissue
      • Histology Positive Control Tissue
      • Histology Positive Control Tissue
      • Histology Positive Control Tissue
      • Histology Positive Control Tissue
      • Histology Positive Control Tissue
      • Histology Positive Control Tissue
      • Histology Positive Control Tissue
      • Histology Positive Control Tissue
      • Histology Positive Control Tissue
      • Histology Positive Control Tissue
      • Histology Positive Control Tissue
      • Histology Positive Control Tissue
      • Histology Positive Control Tissue
      • Immunofluorescence
      • Immunofluorescence
      • Immunofluorescence
      • Immunofluorescence
      • Immunofluorescence
      • Immunofluorescence
      • Immunofluorescence
      • Immunofluorescence
      • Immunofluorescence
      • Immunofluorescence
      • Immunofluorescence
      • Immunofluorescence
      • Immunofluorescence
      • Immunofluorescence
      • Immunofluorescence
      • Immunofluorescence
      • Immunofluorescence
      • Western Blotting
      • Western Blotting
      • Western Blotting
      • Western Blotting
      • Western Blotting
      • Western Blotting
      • Western Blotting
      • Western Blotting
      • Western Blotting
      • Western Blotting
      • Western Blotting
      • Western Blotting
      • Western Blotting
      • Western Blotting
      • Western Blotting
      • Western Blotting
      • Western Blotting
      • Instructions For Use
      • Instructions For Use
      • Instructions For Use
      • Instructions For Use
      • Instructions For Use
      • Instructions For Use
      • Instructions For Use
      • Instructions For Use
      • Instructions For Use
      • Instructions For Use
      • Instructions For Use
      • Instructions For Use
      • Instructions For Use
      • Instructions For Use
      • Instructions For Use
      • Instructions For Use
      • Instructions For Use

      Additional CD68 Antibody Formats

      Formats Clone Applications Sizes available
      CD68 Antibody : Biotin FA-11 C 0.1 mg | 25 µg
      CD68 Antibody : Alexa Fluor® 647 FA-11 F * 100 Tests/1ml | 25 Tests/0.25ml
      CD68 Antibody : FITC FA-11 F * 0.1 mg | 25 µg | 50 µg
      CD68 Antibody : RPE FA-11 F * 100 Tests | 25 Tests
      CD68 Antibody : Purified FA-11 C, F *, IF, IP, P *, WB* 0.25 mg | 25 µg | 0.1 mg
      CD68 Antibody : Alexa Fluor® 488 FA-11 F * 25 Tests/0.25ml | 100 Tests/1ml
      CD68 Antibody : Alexa Fluor® 700 FA-11 F * 100 Tests/1ml | 25 Tests/0.25ml
      CD68 Antibody : Low Endotoxin FA-11 C, F *, IF, IP, P *, WB* 0.5 mg
      • Copyright © 2016 Bio-Rad Antibodies (formerly AbD Serotec)

      Recommended Secondary Antibody

        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Goat anti Rat IgG:Alk. Phos. (Mouse Adsorbed)STAR131A1 mlC, E, P, WB
        STAR131A
        Goat anti Rat IgG:Biotin (Mouse Adsorbed)STAR131B0.5 mgC, E, IF, P, WB
        STAR131B
        Goat anti Rat IgG:DyLight®549 (Mouse Adsorbed)STAR71D549GA0.1 mgF, IF
        STAR71D549GA
        Goat anti Rat IgG:DyLight®649 (Mouse Adsorbed)STAR71D649GA0.1 mgF, IF
        STAR71D649GA
        Rabbit F(ab')2 anti Rat IgG:Dylight®800STAR16D800GA0.1 mgF, IF, WB
        STAR16D800GA
        Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed)STAR71D800GA0.1 mgF, IF, WB
        STAR71D800GA
        Goat F(ab')2 anti Rat IgG:FITC (Mouse Adsorbed)STAR690.5 mlF
        STAR69
        Rabbit F(ab')2 anti Rat IgG:FITCSTAR17B1 mgF
        STAR17B
        Goat anti Rat IgG:HRP (Mouse Adsorbed)STAR720.5 mgC, E, P
        STAR72
        Rabbit F(ab')2 anti Rat IgG:HRPSTAR21B1 mgC, E, P, RE
        STAR21B
        Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed)STAR730.5 mlF
        STAR73
        Rabbit F(ab')2 anti Rat IgG:RPESTAR20A1 mlF
        STAR20A
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Streptavidin:APCSTAR119100 TestsF
        STAR119
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Goat anti Rat IgG:Alk. Phos. (Mouse Adsorbed)STAR131A1 mlC, E, P, WB
        STAR131A
        Goat anti Rat IgG:Biotin (Mouse Adsorbed)STAR131B0.5 mgC, E, IF, P, WB
        STAR131B
        Goat anti Rat IgG:DyLight®549 (Mouse Adsorbed)STAR71D549GA0.1 mgF, IF
        STAR71D549GA
        Goat anti Rat IgG:DyLight®649 (Mouse Adsorbed)STAR71D649GA0.1 mgF, IF
        STAR71D649GA
        Rabbit F(ab')2 anti Rat IgG:Dylight®800STAR16D800GA0.1 mgF, IF, WB
        STAR16D800GA
        Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed)STAR71D800GA0.1 mgF, IF, WB
        STAR71D800GA
        Goat F(ab')2 anti Rat IgG:FITC (Mouse Adsorbed)STAR690.5 mlF
        STAR69
        Rabbit F(ab')2 anti Rat IgG:FITCSTAR17B1 mgF
        STAR17B
        Goat anti Rat IgG:HRP (Mouse Adsorbed)STAR720.5 mgC, E, P
        STAR72
        Rabbit F(ab')2 anti Rat IgG:HRPSTAR21B1 mgC, E, P, RE
        STAR21B
        Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed)STAR730.5 mlF
        STAR73
        Rabbit F(ab')2 anti Rat IgG:RPESTAR20A1 mlF
        STAR20A
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Goat anti Rat IgG:Alk. Phos. (Mouse Adsorbed)STAR131A1 mlC, E, P, WB
        STAR131A
        Goat anti Rat IgG:Biotin (Mouse Adsorbed)STAR131B0.5 mgC, E, IF, P, WB
        STAR131B
        Goat anti Rat IgG:DyLight®549 (Mouse Adsorbed)STAR71D549GA0.1 mgF, IF
        STAR71D549GA
        Goat anti Rat IgG:DyLight®649 (Mouse Adsorbed)STAR71D649GA0.1 mgF, IF
        STAR71D649GA
        Rabbit F(ab')2 anti Rat IgG:Dylight®800STAR16D800GA0.1 mgF, IF, WB
        STAR16D800GA
        Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed)STAR71D800GA0.1 mgF, IF, WB
        STAR71D800GA
        Goat F(ab')2 anti Rat IgG:FITC (Mouse Adsorbed)STAR690.5 mlF
        STAR69
        Rabbit F(ab')2 anti Rat IgG:FITCSTAR17B1 mgF
        STAR17B
        Goat anti Rat IgG:HRP (Mouse Adsorbed)STAR720.5 mgC, E, P
        STAR72
        Rabbit F(ab')2 anti Rat IgG:HRPSTAR21B1 mgC, E, P, RE
        STAR21B
        Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed)STAR730.5 mlF
        STAR73
        Rabbit F(ab')2 anti Rat IgG:RPESTAR20A1 mlF
        STAR20A
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Streptavidin:APCSTAR119100 TestsF
        STAR119
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Goat anti Rat IgG:Alk. Phos. (Mouse Adsorbed)STAR131A1 mlC, E, P, WB
        STAR131A
        Goat anti Rat IgG:Biotin (Mouse Adsorbed)STAR131B0.5 mgC, E, IF, P, WB
        STAR131B
        Goat anti Rat IgG:DyLight®549 (Mouse Adsorbed)STAR71D549GA0.1 mgF, IF
        STAR71D549GA
        Goat anti Rat IgG:DyLight®649 (Mouse Adsorbed)STAR71D649GA0.1 mgF, IF
        STAR71D649GA
        Rabbit F(ab')2 anti Rat IgG:Dylight®800STAR16D800GA0.1 mgF, IF, WB
        STAR16D800GA
        Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed)STAR71D800GA0.1 mgF, IF, WB
        STAR71D800GA
        Goat F(ab')2 anti Rat IgG:FITC (Mouse Adsorbed)STAR690.5 mlF
        STAR69
        Rabbit F(ab')2 anti Rat IgG:FITCSTAR17B1 mgF
        STAR17B
        Goat anti Rat IgG:HRP (Mouse Adsorbed)STAR720.5 mgC, E, P
        STAR72
        Rabbit F(ab')2 anti Rat IgG:HRPSTAR21B1 mgC, E, P, RE
        STAR21B
        Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed)STAR730.5 mlF
        STAR73
        Rabbit F(ab')2 anti Rat IgG:RPESTAR20A1 mlF
        STAR20A

        Recommended Negative Isotype Control

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Rat IgG2a Negative Control:Low EndotoxinMCA1212EL0.5 mgF
          MCA1212EL
          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Rat IgG2a Negative ControlMCA12121 mlE, F
          MCA1212
          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Rat IgG2a Negative Control:FITCMCA1212F100 TestsF
          MCA1212F
          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Rat IgG2a Negative Control:Alexa Fluor® 488MCA1212A488100 Tests/1mlF
          MCA1212A488
          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Rat IgG2a Negative Control:Alexa Fluor® 488MCA1212A488100 Tests/1mlF
          MCA1212A488
          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Rat IgG2a Negative Control:Alexa Fluor® 700MCA1212A700100 Tests/1mlF
          MCA1212A700
          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Rat IgG2a Negative Control:Alexa Fluor® 647MCA1212A647100 Tests/1mlF
          MCA1212A647
          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Rat IgG2a Negative Control:FITCMCA1212F100 TestsF
          MCA1212F
          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Rat IgG2a Negative Control:RPEMCA1212PE100 TestsF
          MCA1212PE
          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Rat IgG2a Negative Control:Alexa Fluor® 700MCA1212A700100 Tests/1mlF
          MCA1212A700
          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Rat IgG2a Negative Control:RPEMCA1212PE100 TestsF
          MCA1212PE
          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Rat IgG2a Negative ControlMCA12121 mlE, F
          MCA1212
          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Rat IgG2a Negative ControlMCA12121 mlE, F
          MCA1212
          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Rat IgG2a Negative Control:Alexa Fluor® 647MCA1212A647100 Tests/1mlF
          MCA1212A647
          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Rat IgG2a Negative Control:FITCMCA1212F100 TestsF
          MCA1212F

          Useful Reagents

            DescriptionProduct CodePack SizeApplicationsList PriceQuantity
            Antigen Retrieval Buffer, pH8.0BUF025C50 mlP
            BUF025C
            Antigen Retrieval Buffer, pH8.0BUF025A500 mlP
            BUF025A
            Leucoperm™BUF0950 TestsF
            BUF09
            DescriptionProduct CodePack SizeApplicationsList PriceQuantity
            Mouse Seroblock FcRBUF041A0.1 mgF
            BUF041A
            Mouse Seroblock FcRBUF041B0.5 mgF
            BUF041B
            Leucoperm™BUF0950 TestsF
            BUF09
            DescriptionProduct CodePack SizeApplicationsList PriceQuantity
            Leucoperm™BUF0950 TestsF
            BUF09
            DescriptionProduct CodePack SizeApplicationsList PriceQuantity
            Leucoperm™BUF0950 TestsF
            BUF09
            DescriptionProduct CodePack SizeApplicationsList PriceQuantity
            Leucoperm™BUF0950 TestsF
            BUF09
            DescriptionProduct CodePack SizeApplicationsList PriceQuantity
            Mouse Seroblock FcRBUF041A0.1 mgF
            BUF041A
            Mouse Seroblock FcRBUF041B0.5 mgF
            BUF041B
            Leucoperm™BUF0950 TestsF
            BUF09
            DescriptionProduct CodePack SizeApplicationsList PriceQuantity
            Leucoperm™BUF0950 TestsF
            BUF09
            DescriptionProduct CodePack SizeApplicationsList PriceQuantity
            Leucoperm™BUF0950 TestsF
            BUF09
            DescriptionProduct CodePack SizeApplicationsList PriceQuantity
            Leucoperm™BUF0950 TestsF
            BUF09
            DescriptionProduct CodePack SizeApplicationsList PriceQuantity
            Leucoperm™BUF0950 TestsF
            BUF09
            DescriptionProduct CodePack SizeApplicationsList PriceQuantity
            Mouse Seroblock FcRBUF041A0.1 mgF
            BUF041A
            Mouse Seroblock FcRBUF041B0.5 mgF
            BUF041B
            Leucoperm™BUF0950 TestsF
            BUF09
            DescriptionProduct CodePack SizeApplicationsList PriceQuantity
            Leucoperm™BUF0950 TestsF
            BUF09
            DescriptionProduct CodePack SizeApplicationsList PriceQuantity
            Antigen Retrieval Buffer, pH8.0BUF025C50 mlP
            BUF025C
            Antigen Retrieval Buffer, pH8.0BUF025A500 mlP
            BUF025A
            Leucoperm™BUF0950 TestsF
            BUF09
            DescriptionProduct CodePack SizeApplicationsList PriceQuantity
            Leucoperm™BUF0950 TestsF
            BUF09
            DescriptionProduct CodePack SizeApplicationsList PriceQuantity
            Leucoperm™BUF0950 TestsF
            BUF09
            DescriptionProduct CodePack SizeApplicationsList PriceQuantity
            Mouse Seroblock FcRBUF041A0.1 mgF
            BUF041A
            Mouse Seroblock FcRBUF041B0.5 mgF
            BUF041B
            Leucoperm™BUF0950 TestsF
            BUF09
            DescriptionProduct CodePack SizeApplicationsList PriceQuantity
            Mouse Seroblock FcRBUF041A0.1 mgF
            BUF041A
            Mouse Seroblock FcRBUF041B0.5 mgF
            BUF041B
            Leucoperm™BUF0950 TestsF
            BUF09

            Recommended Positive Controls

              Histology Controls

                Source Reference

                References

                Write your review

                CD68 FA-11
                5/5stars
                by
                Submitted: 28 Mar 2016
                I work for a histology company that does quite a bit of IHC staining. The CD68 FA-11 clone that is provided by AbD Serotec has been our 'go to' for staining CD68 positive microglia in mouse tissue for many years. We have used it countless times with outstanding results. Thank you for providing this quality product!

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