SARS-CoV Nucleoprotein antibody | 3861
Mouse anti SARS-CoV Nucleoprotein
- Product Type
- Monoclonal Antibody
- Clone
- 3861
- Isotype
- IgG1
- Specificity
- SARS-CoV Nucleoprotein
Antibody Quality and Performance Guarantee
| Mouse anti SARS-CoV nucleoprotein antibody recognizes nucleoprotein, also known as nucleocapsid protein and Protein N. The nucleoprotein (N) is the most abundant viral protein in SARS-CoV-infected cells (Chang et al. 2014). It is one of the four structural proteins essential for viral assembly, alongside the spike (S), envelope (E), and membrane (M) proteins (Bartlam et al. 2005). The nucleoprotein encloses the viral genome; during assembly of the virion, nucleoprotein binds to viral RNA and forms the helical nucleocapsid (Zhu et al. 2004). It appears to be a multifunctional protein, and plays an important role in enhancing viral transcription and assembly (McBride et al. 2014). The N protein of a range of coronaviruses are abundantly expressed and are highly immunogenic, and high concentrations of anti-nucleoprotein IgG antibodies have been detected in patients with SARS. These properties have made the nucleoprotein of SARS-CoV-2 a target of interest for developing a vaccine against COVID-19 (Dutta et al. 2020). |
- Target Species
- Viral
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from ascites
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Immunogen
- Recombinant fragment aa1-49 of the SARS nucleoprotein
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
10 ug/ml | 1.0 ug/ml |
| Western Blotting | |
1.0 ug/ml |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- UniProt
- P59595
- GO Terms
- GO:0003723 RNA binding
- GO:0030529 ribonucleoprotein complex
- GO:0005198 structural molecule activity
- GO:0019013 viral nucleocapsid
- GO:0044172 host cell endoplasmic reticulum-Golgi intermediate compartment
- GO:0044177 host cell Golgi apparatus
- GO:0044220 host cell perinuclear region of cytoplasm
MCA6372
156961
If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
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