Dengue Virus antibody | Dengue 1-11(3)

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Mouse anti Dengue Virus

Product Type
Monoclonal Antibody
Clone
Dengue 1-11(3)
Isotype
IgG2a
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA2277 E IF P* WB 0.25 mg
Mouse anti-Dengue virus antibody, clone Dengue 1-11 (3) recognizes all four dengue virus serotypes (DEN-1, DEN-2, DEN-3 and DEN-4) of the genus Flavivirus.

Mouse anti-Dengue virus antibody, clone Dengue 1-11 (3) binds strongly to subtypes 1 and 2. It recognizes subtype 3 more weakly. Among the four subtypes, subtype 4 is recognized the least strongly and some customers have reported no recognition of subtype 4.

The dengue virus is responsible for the tropical and sub-tropical diseases, dengue (DF) and dengue haemorrhagic fever (DHF), transmitted to individuals through the bite of the Aedes mosquito. The global distribution of dengue is expanding and comparable to that of malaria, with symptoms ranging from a mild viral syndrome of high fever, rash, headache, fatigue and muscle and joint pain, to the more severe and sometimes fatal DHF. Each dengue virus serotype is antigenically distinct, such that infection only provides an individual with immunity to the causative serotype, with the possibility of further infection by a different serotype or even multiple serotypes.

Product Details

Target Species
Viral
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.05%Sodium Azide (NaN3)
Approx. Protein Concentrations
IgG concentration 1.0mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

Regulatory
For research purposes only

Applications of Dengue Virus antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Immunofluorescence
Immunohistology - Paraffin 1
Western Blotting
  1. 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
This antibody detects an anti-E/envelope specific band of 61kDa under reducing conditions in Western blotting. A weak secondary band of 80kDa may also be apparent.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Human anti Mouse IgG2a:FITC HCA037F 0.1 mg F
Human anti Mouse IgG2a:HRP HCA037P 0.1 mg E
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A 0.5 mg E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed) STAR117D549GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed) STAR117D649GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA 0.1 mg F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F 0.5 mg F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P 0.5 mg E WB
Goat anti Mouse IgG (Fc):FITC STAR120F 1 mg C F
Goat anti Mouse IgG (Fc):HRP STAR120P 1 mg E WB
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A 1 ml F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B 1 mg C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 0.5 mg F
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 1 ml F
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 0.5 mg C E P
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A 1 mg C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P 1 mg E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA 0.1 mg F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B 1 mg F

Product Specific References

References for Dengue Virus antibody

  1. Gubler, D.J. (1998) Dengue and dengue hemorrhagic fever.
    Clin Microbiol Rev. 11 (3): 480-96.
  2. PhilipSamuel, P. & Tyagi, B.K. (2006) Diagnostic methods for detection & isolation of dengue viruses from vector mosquitoes.
    Indian J Med Res. 123 (5): 615-28.
  3. Loroño-pino, M.A. et al. (1999) Common occurrence of concurrent infections by multiple dengue virus serotypes.
    Am J Trop Med Hyg. 61 (5): 725-30.
  4. Kim,T.G. et al. (2010) Cholera toxin B subunit-domain III of dengue virus envelope glycoprotein E fusion protein production in transgenic plants.
    Protein Expr Purif. 74:236-41.
  5. Milic NL et al. (2015) Sequence analysis and characterisation of virally induced viperin in the saltwater crocodile (Crocodylus porosus).
    Dev Comp Immunol. pii: S0145-305X(15)00044-0.
  6. Poggianella, M. et al. (2015) Dengue E Protein Domain III-Based DNA Immunisation Induces Strong Antibody Responses to All Four Viral Serotypes.
    PLoS Negl Trop Dis. 9 (7): e0003947.
  7. Nguyen, N.L. et al. (2015) Expression and characterization of an M cell-specific ligand-fused dengue virus tetravalent epitope using Saccharomyces cerevisiae.
    J Biosci Bioeng. 119 (1): 19-27.
  8. Slon Campos, J.L. (2017) Evaluation of a Tetravalent DNA Vaccine against Dengue: Integrating Biochemical Studies on Dengue Virus Envelope Protein to a Domain-Based Antigen Design.
    PhD thesis The Open University.