Dengue Virus antibody | Dengue 1-11(3)
Mouse anti-Dengue virus antibody, clone Dengue 1-11 (3) binds strongly to subtypes 1 and 2. It recognizes subtype 3 more weakly. Among the four subtypes, subtype 4 is recognized the least strongly and some customers have reported no recognition of subtype 4.
The dengue virus is responsible for the tropical and sub-tropical diseases, dengue (DF) and dengue haemorrhagic fever (DHF), transmitted to individuals through the bite of the Aedes mosquito. The global distribution of dengue is expanding and comparable to that of malaria, with symptoms ranging from a mild viral syndrome of high fever, rash, headache, fatigue and muscle and joint pain, to the more severe and sometimes fatal DHF. Each dengue virus serotype is antigenically distinct, such that infection only provides an individual with immunity to the causative serotype, with the possibility of further infection by a different serotype or even multiple serotypes.
- Target Species
- Product Form
- Purified IgG - liquid
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- <0.1% Sodium Azide (NaN3)
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
- 12 months from date of despatch
- For research purposes only
Applications of Dengue Virus antibody
|Application Name||Verified||Min Dilution||Max Dilution|
|Immunohistology - Paraffin 1|
- 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
- Western Blotting
- This antibody detects an anti-E/envelope specific band of 61kDa under reducing conditions in Western blotting. A weak secondary band of 80kDa may also be apparent.
Secondary Antibodies Available
Product Specific References
References for Dengue Virus antibody
Poggianella, M. et al. (2015) Dengue E Protein Domain III-Based DNA Immunisation Induces Strong Antibody Responses to All Four Viral Serotypes.
PLoS Negl Trop Dis. 9 (7): e0003947.
Slon Campos, J.L. (2017) Evaluation of a Tetravalent DNA Vaccine against Dengue: Integrating Biochemical Studies on Dengue Virus Envelope Protein to a Domain-Based Antigen Design.
PhD thesis The Open University.
Nguyen, N.L. et al. (2015) Expression and characterization of an M cell-specific ligand-fused dengue virus tetravalent epitope using Saccharomyces cerevisiae.
J Biosci Bioeng. 119 (1): 19-27.
Kim,T.G. et al. (2010) Cholera toxin B subunit-domain III of dengue virus envelope glycoprotein E fusion protein production in transgenic plants.
Protein Expr Purif. 74:236-41.
Milic, N.L. et el. (2015) Sequence analysis and characterisation of virally induced viperin in the saltwater crocodile (Crocodylus porosus).
Dev Comp Immunol. 51 (1): 108-15.
Kim, M.Y. et al. (2018) Plant-expressed Fc-fusion protein tetravalent dengue vaccine with inherent adjuvant properties.
Plant Biotechnol J. 16 (7): 1283-94.
Gubler, D.J. (1998) Dengue and dengue hemorrhagic fever.
Clin Microbiol Rev. 11 (3): 480-96.
Loroño-pino, M.A. et al. (1999) Common occurrence of concurrent infections by multiple dengue virus serotypes.
Am J Trop Med Hyg. 61 (5): 725-30.
PhilipSamuel, P. & Tyagi, B.K. (2006) Diagnostic methods for detection & isolation of dengue viruses from vector mosquitoes.
Indian J Med Res. 123 (5): 615-28.
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