Dengue Virus antibody | Dengue 1-11(3)

Mouse anti Dengue Virus

Product Type
Monoclonal Antibody
Clone
Dengue 1-11(3)
Isotype
IgG2a
Specificity
Dengue Virus

Product Code Applications Pack Size List Price Your Price Qty
MCA2277
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E IF P * WB 0.25 mg loader
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loader
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Mouse anti-Dengue virus antibody, clone Dengue 1-11 (3) recognizes all four dengue virus serotypes (DEN-1, DEN-2, DEN-3 and DEN-4) of the genus Flavivirus.

Mouse anti-Dengue virus antibody, clone Dengue 1-11 (3) binds strongly to subtypes 1 and 2. It recognizes subtype 3 more weakly. Among the four subtypes, subtype 4 is recognized the least strongly and some customers have reported no recognition of subtype 4.

The dengue virus is responsible for the tropical and sub-tropical diseases, dengue (DF) and dengue haemorrhagic fever (DHF), transmitted to individuals through the bite of the Aedes mosquito. The global distribution of dengue is expanding and comparable to that of malaria, with symptoms ranging from a mild viral syndrome of high fever, rash, headache, fatigue and muscle and joint pain, to the more severe and sometimes fatal DHF. Each dengue virus serotype is antigenically distinct, such that infection only provides an individual with immunity to the causative serotype, with the possibility of further infection by a different serotype or even multiple serotypes.

Target Species
Viral
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
<0.1% Sodium Azide (NaN3)
Approx. Protein Concentrations
IgG concentration 1.0mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Immunofluorescence
Immunohistology - Paraffin 1
Western Blotting
  1. 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
This antibody detects an anti-E/envelope specific band of 61kDa under reducing conditions in Western blotting. A weak secondary band of 80kDa may also be apparent.

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Description Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):DyLight®550 (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):DyLight®650 (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (Fc):FITC
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Description Goat anti Mouse IgG (Fc):HRP
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Description Rabbit F(ab')2 anti Mouse IgG:RPE
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Description Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 F 0.5 mg loader
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Description Goat anti Mouse IgG:FITC (Rat Adsorbed)
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Description Goat anti Mouse IgG:RPE (Rat Adsorbed)
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Description Goat anti Mouse IgG:HRP (Rat Adsorbed)
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A C E WB 1 mg loader
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Description Goat anti Mouse IgG/A/M:Alk. Phos.
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P E 1 mg loader
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Description Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA F IF WB 0.1 mg loader
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Description Rabbit F(ab')2 anti Mouse IgG:Dylight®800
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Description Rabbit F(ab')2 anti Mouse IgG:FITC

References for Dengue Virus antibody

  1. Poggianella, M. et al. (2015) Dengue E Protein Domain III-Based DNA Immunisation Induces Strong Antibody Responses to All Four Viral Serotypes.
    PLoS Negl Trop Dis. 9 (7): e0003947.
  2. Slon Campos, J.L. (2017) Evaluation of a Tetravalent DNA Vaccine against Dengue: Integrating Biochemical Studies on Dengue Virus Envelope Protein to a Domain-Based Antigen Design.
    PhD thesis The Open University.
  3. Nguyen, N.L. et al. (2015) Expression and characterization of an M cell-specific ligand-fused dengue virus tetravalent epitope using Saccharomyces cerevisiae.
    J Biosci Bioeng. 119 (1): 19-27.
  4. Kim,T.G. et al. (2010) Cholera toxin B subunit-domain III of dengue virus envelope glycoprotein E fusion protein production in transgenic plants.
    Protein Expr Purif. 74:236-41.
  5. Milic, N.L. et el. (2015) Sequence analysis and characterisation of virally induced viperin in the saltwater crocodile (Crocodylus porosus).
    Dev Comp Immunol. 51 (1): 108-15.
  6. Kim, M.Y. et al. (2018) Plant-expressed Fc-fusion protein tetravalent dengue vaccine with inherent adjuvant properties.
    Plant Biotechnol J. 16 (7): 1283-94.
  7. Kim, B. & Kim, M. (2019) Evaluation of the oral immunogenicity of M cell-targeted tetravalent EDIII antigen for development of plant-based edible vaccine against dengue infection
    Plant Cell, Tissue and Organ Culture (PCTOC). 137 (1): 1-10.

Further Reading

  1. Gubler, D.J. (1998) Dengue and dengue hemorrhagic fever.
    Clin Microbiol Rev. 11 (3): 480-96.
  2. Loroño-pino, M.A. et al. (1999) Common occurrence of concurrent infections by multiple dengue virus serotypes.
    Am J Trop Med Hyg. 61 (5): 725-30.
  3. PhilipSamuel, P. & Tyagi, B.K. (2006) Diagnostic methods for detection & isolation of dengue viruses from vector mosquitoes.
    Indian J Med Res. 123 (5): 615-28.

RRID
AB_609623
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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