Cytomegalovirus gB Late Cytoplasmic Antigen antibody | hCMV34

Mouse anti Cytomegalovirus gB Late Cytoplasmic Antigen

Product Type
Monoclonal Antibody
Clone
hCMV34
Isotype
IgG2a
Specificity
Cytomegalovirus gB Late Cytoplasmic Antigen

Product Code Applications Pack Size List Price Your Price Qty
2470-5416
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C * E IF 0.2 mg loader
List Price Your Price
loader
Search for Batch Specific Datasheets

Mouse anti Cytomegalovirus gB Late Cytoplasmic Antigen antibody, clone hCMV34 detects cytomegalovirus glycoprotein B late cytoplasmic antigen. Cytomegalovirus is an enveloped double stranded DNA virus which in humans (HCMV) is commonly known as human herpesvirus 5 (HHV-5).

Glycoprotein B (gB) is the main antigenic protein within the envelope of HCMV. It is required for HCMV mediated membrane fusion along with the gH / gL complex. The unprocessed precursor of HCMV gB is 906 amino acids in length, with a MW of approximately 100kDa.

Mouse anti Cytomegalovirus gB Late Cytoplasmic Antigen antibody, clone hCMV34 does not react with HSV, VZV or EBV. Reacts with MRC-5 infected cells, but not uninfected cells.

Mouse anti Cytomegalovirus gB Late Cytoplasmic Antigen antibody, clone hCMV34 has been used successfully to demonstrate presence of CMV in infected cells by immunofluorescence on paraformaldehyde fixed cell cultures (González-Sánchez et al 2015).

Target Species
Viral
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Carrier Free
Yes
Immunogen
Native cytomeglaovirus extracts from infected human cells..
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Immunofluorescence 0.02 µg/slide 1 µg/slide
Immunohistology - Frozen 1
  1. 1Reactive only in acetone fixed CMV infected cells or frozen sections.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.

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References for Cytomegalovirus gB Late Cytoplasmic Antigen antibody

  1. González-Sánchez HM et al. (2015) Effects of cytomegalovirus infection in human neural precursor cells depend on their differentiation state.
    J Neurovirol. 21 (4): 346-57.

Synonyms
CMV
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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