Adenovirus antibody | B025 (AD51)

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Mouse anti Adenovirus

Adenovirus antibody, clone B025 (AD51) recognizes all human adenoviruses serotypes. The most common infections caused by adenovirus are respiratory tract, skin-rash, stomach and bladder.

Product Type
Monoclonal Antibody
Clone
B025 (AD51)
Isotype
IgG2a
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA489 C E P* 1 mg
Mouse anti adenovirus antibody, clone B025 (AD51) recognizes all human adenoviruses serotypes.

The most common infections caused by adenovirus are respiratory tract infections but some infections may also lead to conjunctivitis, skin-rash, diarrhea and bladder infections. Infant and children are most commonly affected by adenoviruses.

Adenoviruses are icosahedral non-enveloped linear double-stranded DNA viruses. There are at least 51 serotypes, which are categorized into 6 species (A-F) based on molecular criteria. The virus capsid is composed of three different proteins: 12 fiber attachment proteins associated with 12 penton base proteins and 240 hexon proteins, which form the main capsid component (Ebner et al. 2005).

Mouse anti adenovirus antibody, clone B025 (AD51) reacts with the adenovirus specific hexon polypeptide.

Due to their infectivity to both quiescent and proliferating cells, adenoviruses have also been used as vectors in vaccination and in gene therapy (Thomas et al. 2002 and Abad et al. 2002).

Product Details

Target Species
Viral
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Immunogen
Adenovirus type 3 (ATCC strain VR847).
Purity
>90% IgG content by SDS page
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the JK.Ag8.653 mouse myeloma cell line.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

Regulatory
For research purposes only

Applications of Adenovirus antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Immunohistology - Frozen
Immunohistology - Paraffin 1
  1. 1This product requires protein digestion pre-treatment of paraffin sections e.g. trypsin or pronase.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Human anti Mouse IgG2a:FITC HCA037F 0.1 mg F
Human anti Mouse IgG2a:HRP HCA037P 0.1 mg E
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A 0.5 mg E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed) STAR117D549GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed) STAR117D649GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA 0.1 mg F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F 0.5 mg F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P 0.5 mg E WB
Goat anti Mouse IgG (Fc):FITC STAR120F 1 mg C F
Goat anti Mouse IgG (Fc):HRP STAR120P 1 mg E WB
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A 1 ml F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B 1 mg C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 0.5 mg F
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 1 ml F
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 0.5 mg C E P
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A 1 mg C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P 1 mg E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA 0.1 mg F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B 1 mg F

Application Based External Images

Immunohistology - Frozen

Immunohistology - Paraffin

Product Specific References

References for Adenovirus antibody

  1. Maddox, A. et al. (1992) Adenovirus infection of the large bowel in HIV positive patients.
    J Clin Pathol. 45 (8): 684-8.
  2. Thomas, C.E. et al. (2002) Adenovirus binding to the coxsackievirus and adenovirus receptor or integrins is not required to elicit brain inflammation but is necessary to transduce specific neural cell types.
    J Virol. 76: 3452-60.
  3. Abad, L.W. et al. (2002) Development of a biosensor-based method for detection and isotyping of antibody responses to adenoviral-based gene therapy vectors.
    Anal Biochem. 2002 Nov 1;310(1):107-13.
  4. Audu, R. et al. (2002) Isolation and identification of adenovirus recovered from the stool of children with diarrhoea in Lagos, Nigeria.
    Afr J Health Sci. 9: 105-11.
  5. Griesche, N. et al. (2008) Growth characteristics of human adenoviruses on porcine cell lines.
    Virology. 373: 400-10.
  6. Blanshard, C. and Gazzard, B.G. (1995) Natural history and prognosis of diarrhoea of unknown cause in patients with acquired immunodeficiency syndrome (AIDS).
    Gut. 36: 283-6.
  7. Thomas, P.D. et al. (2001) Enteric viral infections as a cause of diarrhoea in the acquired immunodeficiency syndrome.
    HIV Med. 1: 19-24.
  8. Blanshard, C. et al. (1996) Investigation of chronic diarrhoea in acquired immunodeficiency syndrome. A prospective study of 155 patients.
    Gut. 39: 824-32.
  9. Morfin, F. et al. (2005) In vitro susceptibility of adenovirus to antiviral drugs is species-dependent.
    Antivir Ther. 10: 225-9.