Adenovirus antibody | B025 (AD51)
Biotinylated Mouse anti adenovirus antibody, clone B025 (AD51) (MCA489 used for the immunomagnetic concentration of human mastadenovirus from environmetal water samples.
Image caption:
Magnetic bead surface functionalization with antibodies and immunomagnetic separation (IMS) procedure.
From: Gularte, J.S.; de Oliveira Hansen, R.; Demoliner, M.; Fiutowski, J.; Eisen, A.K.A.; Heldt, F.H.; Rodrigues de Almeida, P.; Müller de Quevedo, D.; Rubahn, H.-G.; Rosado Spilki, F.
Functionalized Surfaces as a Tool for Virus Sensing: A Demonstration of Human mastadenovirus Detection in Environmental Waters.
Chemosensors 2021, 9, 19.
doi:10.3390/chemosensors9020019
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Adenovirus
- Product Type
- Monoclonal Antibody
- Clone
- B025 (AD51)
- Isotype
- IgG2a
- Specificity
- Adenovirus
Antibody Quality and Performance Guarantee
| Mouse anti adenovirus antibody, clone B025 (AD51) recognizes all human adenoviruses serotypes. The most common infections caused by adenovirus are respiratory tract infections but some infections may also lead to conjunctivitis, skin-rash, diarrhea and bladder infections. Infant and children are most commonly affected by adenoviruses. Adenoviruses are icosahedral non-enveloped linear double-stranded DNA viruses. There are at least 51 serotypes, which are categorized into 6 species (A-F) based on molecular criteria. The virus capsid is composed of three different proteins: 12 fiber attachment proteins associated with 12 penton base proteins and 240 hexon proteins, which form the main capsid component (Ebner et al. 2005). Mouse anti adenovirus antibody, clone B025 (AD51) reacts with the adenovirus specific hexon polypeptide. Due to their infectivity to both quiescent and proliferating cells, adenoviruses have also been used as vectors in vaccination and in gene therapy (Thomas et al. 2002 and Abad et al. 2002). |
- Target Species
- Viral
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- <0.1% Sodium Azide (NaN3)
- Immunogen
- Adenovirus type 3.
- Purity
- >90% IgG content by SDS page
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised BALB/c mice were fused with cells of the JK.Ag8.653 mouse myeloma cell line.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
||
| Immunohistology - Frozen | |
||
| Immunohistology - Paraffin 1 | |
- 1This product requires protein digestion pre-treatment of paraffin sections e.g. trypsin or pronase.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
References for Adenovirus antibody
-
Maddox, A. et al. (1992) Adenovirus infection of the large bowel in HIV positive patients.
J Clin Pathol. 45 (8): 684-8. -
Blanshard, C. and Gazzard, B.G. (1995) Natural history and prognosis of diarrhoea of unknown cause in patients with acquired immunodeficiency syndrome (AIDS).
Gut. 36: 283-6. -
Blanshard, C. et al. (1996) Investigation of chronic diarrhoea in acquired immunodeficiency syndrome. A prospective study of 155 patients.
Gut. 39: 824-32. -
Thomas, P.D. et al. (2001) Enteric viral infections as a cause of diarrhoea in the acquired immunodeficiency syndrome.
HIV Med. 1: 19-24. -
Audu, R. et al. (2002) Isolation and identification of adenovirus recovered from the stool of children with diarrhoea in Lagos, Nigeria.
Afr J Health Sci. 9: 105-11. -
Abad, L.W. et al. (2002) Development of a biosensor-based method for detection and isotyping of antibody responses to adenoviral-based gene therapy vectors.
Anal Biochem. 2002 Nov 1;310(1):107-13. -
Thomas, C.E. et al. (2002) Adenovirus binding to the coxsackievirus and adenovirus receptor or integrins is not required to elicit brain inflammation but is necessary to transduce specific neural cell types.
J Virol. 76: 3452-60. -
Morfin, F. et al. (2005) In vitro susceptibility of adenovirus to antiviral drugs is species-dependent.
Antivir Ther. 10: 225-9.
View The Latest Product References
-
Griesche, N. et al. (2008) Growth characteristics of human adenoviruses on porcine cell lines.
Virology. 373: 400-10. -
Gularte, S.J. et al. (2021) Functionalized Surfaces as a Tool for Virus Sensing: A Demonstration of Human mastadenovirus Detection in Environmental Waters
Chemosensors. 9 (2): 19.
- RRID
- AB_321166
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