CD31 antibody | CO.3E1D4

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Mouse anti Sheep CD31:FITC

Mouse anti Sheep CD31

Product Type
Monoclonal Antibody
Clone
CO.3E1D4
Isotype
IgG2a
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA1097F F 0.1 mg
MCA1097GA F IP 0.1 mg
Mouse anti Sheep CD31 antibody, clone CO.3E1D4 recognizes ovine CD31, also known as PECAM-1.

Ovine CD31 is predominantly expressed by peripheral blood platelets and a small percentage of lymphocytes. CD31 is also highly expressed by ovine endothelial cells.

Mouse anti Sheep CD31 antibody, clone CO.3E1D4 is reported to inhibit homotypic leucocyte aggregation induced by anti CD43 antibodies (Pintado et al. 1995).

Product Details

Target Species
Sheep
Species Cross-Reactivity
Target SpeciesCross Reactivity
Goat
Bovine
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Carrier Free
Yes
Immunogen
Ovine leucocytes.
Approx. Protein Concentrations
IgG concentration 0.1 mg/ml
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the SP2-0/Ag14 mouse myeloma cell line.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.

Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Shelf Life
18 months from date of despatch.

More Information

Regulatory
For research purposes only

Applications of CD31 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat
Flow Cytometry 1/10 1/25
Immunoprecipitation
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1 x 106 cells in 100ul.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1 x 106 cells in 100ul.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Human anti Mouse IgG2a:FITC HCA037F 0.1 mg F
Human anti Mouse IgG2a:HRP HCA037P 0.1 mg E
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A 0.5 mg E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed) STAR117D549GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed) STAR117D649GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA 0.1 mg F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F 0.5 mg F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P 0.5 mg E WB
Goat anti Mouse IgG (Fc):FITC STAR120F 1 mg C F
Goat anti Mouse IgG (Fc):HRP STAR120P 1 mg E WB
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A 1 ml F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B 1 mg C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 0.5 mg F
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 1 ml F
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 0.5 mg C E P
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A 1 mg C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P 1 mg E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA 0.1 mg F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B 1 mg F

Negative Isotype Controls Available

Description Product Code Pack Size Applications List Price Quantity
Mouse IgG2a Negative Control:FITC MCA929F 100 Tests F

Application Based External Images

Flow Cytometry

Immunofluorescence

Immunohistology - Frozen

Product Specific References

References for CD31 antibody

  1. Brodersen, R. et al. (1998) Analysis of the immunological cross reactivities of 213 well characterized monoclonal antibodies with specificities against various leucocyte surface antigens of human and 11 animal species:
    Vet. Immunol. Immunopathol. 64: 1-13.
  2. Pintado. C. O. et al. (1995) A monoclonal antibody to an ovine gp130 molecule inhibits homotypic aggregation induced by anti CD43 monoclonal antibodies of ruminant leukocytes.
    Immunol. Lett. 45: 81 - 85.
  3. Zannettino, A.C. et al. (2010) Comparative assessment of the osteoconductive properties of different biomaterials in vivo seeded with human or ovine mesenchymal stem/stromal cells.
    Tissue Eng Part A. 16 (12): 3579-87.
  4. Newland, A. et al. (2004) Ovine dendritic cells transduced with an adenoviral CTLA4eEGFP fusion protein construct induce hyporesponsiveness to allostimulation.
    Immunology. 113: 310-7.
  5. De Visscher, G. et al. (2010) Selection of an immunohistochemical panel for cardiovascular research in sheep.
    Appl Immunohistochem Mol Morphol. 18: 382-91.
  6. Filby,. C.E. et al. (2010) Partial pulmonary embolization disrupts alveolarization in fetal sheep.
    Respir Res. 11: 42.
  7. Berardinelli, P. et al. (2013) Role of amniotic fluid mesenchymal cells engineered on MgHA/collagen-based scaffold allotransplanted on an experimental animal study of sinus augmentation.
    Clin Oral Investig. 17 (7): 1661-75.
  8. Summers, C. et al. (2005) An influx of macrophages is the predominant local immune response in ovine pulmonary adenocarcinoma.
    Vet Immunol Immunopathol. 106 (3-4): 285-94.
  9. Lasecka L et al. (2015) Antibodies to the core proteins of nairobi sheep disease virus/ganjam virus reveal details of the distribution of the proteins in infected cells and tissues.
    PLoS One. 10 (4): e0124966.
  10. Boos, A.M. et al. (2011) Directly auto-transplanted mesenchymal stem cells induce bone formation in a ceramic bone substitute in an ectopic sheep model.
    J Cell Mol Med. 15 (6): 1364-78.
  11. van Spreeuwel, A.C.C. (2008) Obtaining pure ovine endothelial and myofibroblast cell cultures
    BMTE 08.49
  12. Iablonskii, P. et al. (2015) Tissue-engineered mitral valve: morphology and biomechanics †.
    Interact Cardiovasc Thorac Surg. 20 (6): 712-9; discussion 719.
  13. Weigand, A. et al. (2017) Bone Tissue Engineering Under Xenogeneic-Free Conditions in a Large Animal Model as a Basis for Early Clinical Applicability.
    Tissue Eng Part A. 23 (5-6): 208-22.