MHC Class II RT1B antibody | OX-6

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Anti Rat MHC Class II RT1B Antibody, clone OX-6 thumbnail image 1

Anti Rat MHC Class II RT1B Antibody, clone OX-6 thumbnail image 2

Anti Rat MHC Class II RT1B Antibody, clone OX-6 thumbnail image 3

Anti Rat MHC Class II RT1B Antibody, clone OX-6 thumbnail image 4

Anti Rat MHC Class II RT1B Antibody, clone OX-6 gallery image 1 — Figure A. FITC conjugated Mouse anti Rat CD45RA (MCA340FT) and RPE conjugated Mouse IgG1 isotype control (MCA1029PE). Figure B. FITC conjugated Rat anti Mouse CD45RA (MCA340FT) and RPE conjugated Mouse anti Rat MHC-II (MCA46PE). All experiments performed on red cell lysed rat blood gated on lymphoid cells in the presence of 10% rat serum. Data acquired on the ZE5™ Cell Analyzer.

Anti Rat MHC Class II RT1B Antibody, clone OX-6 gallery image 2 — Figure A. FITC conjugated mouse anti rat CD45RC (MCA53FT) and RPE conjugated mouse IgG1 isotype control (MCA1209PE). Figure B. FITC conjugated mouse anti rat CD45RC (MCA53FT) and RPE conjugated mouse anti rat RT1B (**MCA46PE**). All experiments performed on red cell lysed rat blood gated on mononuclear cells.

Anti Rat MHC Class II RT1B Antibody, clone OX-6 gallery image 3 — Figure A. RPE conjugated mouse anti rat RT1B (**MCA46PE**) and FITC conjugated mouse IgG1 isotype control (MCA1209F). Figure B. RPE conjugated mouse anti rat RT1B (**MCA46PE**) and FITC conjugated mouse anti rat CD45RC (MCA53FT). All experiments performed on red cell lysed rat blood gated on mononuclear cells.

Anti Rat MHC Class II RT1B Antibody, clone OX-6 gallery image 4 — **Published customer image:**
Mouse anti Rat MHC Class II RT1B, clone OX-6 (**MCA46R**) used for the identification of MHC II expressing microglia in rat retinal sections by immunofluorescence.
**Image caption:**
Effect of LPS on retinal microglia in SD and P23H rats.
Representative vertical retinal sections immunostained with antibodies against Iba1 (green) and MHC-II (red) in vehicle- and LPS-injected SD rats a–f, or vehicle- and LPS-injected P23H rats g–l. Arrows point microglia MHC-II-immunopositive cells. Nuclei were stained with TO-PRO (blue). All images were taken in the central retina. GCL ganglion cell layer, IPL inner plexiform layer, OPL outer plexiform layer. Scale bar 40μm

From: Noailles A, Maneu V, Campello L, Lax P, Cuenca N.
Systemic inflammation induced by lipopolysaccharide aggravates inherited retinal dystrophy.
Cell Death Dis. 2018 Mar 2;9(3):350.

Mouse anti Rat MHC Class II RT1B:Alexa Fluor® 647

Mouse anti Rat MHC Class II RT1B:Biotin

Mouse anti Rat MHC Class II RT1B:FITC

Mouse anti Rat MHC Class II RT1B

Mouse anti Rat MHC Class II RT1B:RPE

Product Type: Monoclonal Antibody
Clone: OX-6
Isotype: IgG1

Product Code	Applications	Pack Size
MCA46A647	F	100 Tests/1ml
MCA46B	F	0.1 mg
MCA46FA	F	50 µg
MCA46FT	F	0.1 mg
MCA46GA	C F IF P*	0.1 mg
MCA46R	C F IF P*	0.25 mg
MCA46G	C F IF P*	1 mg
MCA46PE	F	100 Tests

Mouse anti Rat MHC Class II RT1B antibody, clone OX-6 recognizes a monomorphic determinant of the rat RT1B MHC class II antigen present on B lymphocytes, dendritic cells, some macrophages and certain epithelial cells.

Rat MHC Class II RT1B antibody, clone OX-6 does not react with the rat BDIX strain due to a defect in RT1B expression (Male et al. 1987).

The major histocompatibility complex (MHC) is a cluster of genes that are important in the immune response to infections. In rats, this complex is referred to as the RT1 region. In mice, this complex is referred to as the H-2 region.

Mouse anti Rat MHC Class II RT1B antibody, clone OX-6 also cross reacts with a polymorphic determinant on mouse strains of the H-2 haplotypes k and s. Analysis of recombinant mouse strains has mapped the OX-6 determinant to the H-2I-A region (McMaster and Williams 1979 and Male et al. 1987).

Mouse anti Rat MHC Class II RT1B antibody, clone OX-6 is routinely tested in flow cytometry on rat splenocytes.

Product Details

Target Species

Rat

Species Cross-Reactivity

Target Species	Cross Reactivity
Mouse

N.B. Antibody reactivity and working conditions may vary between species.

Product Form

Purified IgG conjugated to Alexa Fluor® 647 - liquid

Product Form

Purified IgG conjugated to Biotin - liquid

Product Form

Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid

Product Form

Purified IgG - liquid

Product Form

Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized

Reconstitution

Reconstitute with 1 ml distilled water

Preparation

Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant

Preparation

Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant

Preparation

Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant

Preparation

Purified IgG prepared by affinity chromatography on Protein A

Buffer Solution

Phosphate buffered saline

Buffer Solution

Phosphate buffered saline

Buffer Solution

Phosphate buffered saline

Buffer Solution

Phosphate buffered saline

Buffer Solution

Phosphate buffered saline

Preservative Stabilisers

0.09%	Sodium Azide
1%	Bovine Serum Albumin

Preservative Stabilisers

0.09%	Sodium Azide
1%	Bovine Serum Albumin

Preservative Stabilisers

0.09%	Sodium Azide
1%	Bovine Serum Albumin

Preservative Stabilisers

0.09%

Sodium Azide

Preservative Stabilisers

0.09%	Sodium Azide
1%	Bovine Serum Albumin
5%	Sucrose

Carrier Free

Yes

Immunogen

Rat thymocyte membrane glycoproteins.

Approx. Protein Concentrations

IgG concentration 0.05 mg/ml

Approx. Protein Concentrations

IgG concentration 0.1 mg/ml

Approx. Protein Concentrations

IgG concentration 0.1 mg/ml

Approx. Protein Concentrations

IgG concentration 1.0 mg/ml

Fusion Partners

Spleen cells from immunised BALB/c mice were fused with cells from the NS1 mouse myeloma cell line.

Storage Information

Storage: Store at +4^oC or at -20^oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.

Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage: Store at +4^oC or at -20^oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage: Store at +4^oC or at -20^oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.

Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage: Store at +4^oC or at -20^oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage: Prior to reconstitution store at +4^oC. Following reconstitution store at +4^oC.

DO NOT FREEZE.

This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee: 18 months from date of despatch.
Guarantee: 12 months from date of despatch.
Guarantee: 18 months from date of despatch.
Guarantee: 18 months from date of despatch.
Guarantee: 12 months from date of reconstitution.

More Information

Acknowledgements: This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
Regulatory: For research purposes only

Applications of MHC Class II RT1B antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Min Dilution	Max Dilution
Flow Cytometry	Neat	1/10
Flow Cytometry	Neat	1/10
Flow Cytometry	Neat	1/10
Flow Cytometry	1/50	1/100
Immunofluorescence
Immunohistology - Frozen
Immunohistology - Paraffin ¹	1/50	1/100
Flow Cytometry	Neat	1/2

¹This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose. PLP fixation is recommended for optimal results.

Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Flow Cytometry: Use 10ul of the suggested working dilution to label 10⁶ cells in 100ul.
Flow Cytometry: Use 10ul of the suggested working dilution to label 10⁶ cells in 100ul.
Flow Cytometry: Use 10ul of the suggested working dilution to label 10⁶ cells in 100ul.
Flow Cytometry: Use 10ul of the suggested working dilution to label 10⁶ cells in 100ul.
Flow Cytometry: Use 10ul of the suggested working dilution to label 10⁶ cells in 100ul.

Secondary Antibodies Available

Description	Product Code	Pack Size	Applications
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)	STAR117A	0.5 mg	E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)	STAR117D488GA	0.1 mg	F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed)	STAR117D549GA	0.1 mg	F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed)	STAR117D649GA	0.1 mg	F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)	STAR117D680GA	0.1 mg	F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)	STAR117D800GA	0.1 mg	F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)	STAR117F	0.5 mg	F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)	STAR117P	0.5 mg	E WB
Goat anti Mouse IgG (Fc):FITC	STAR120F	1 mg	C F
Goat anti Mouse IgG (Fc):HRP	STAR120P	1 mg	E WB
Rabbit F(ab')2 anti Mouse IgG:RPE	STAR12A	1 ml	F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)	STAR13B	1 mg	C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed)	STAR70	0.5 mg	F
Goat anti Mouse IgG:RPE (Rat Adsorbed)	STAR76	1 ml	F
Goat anti Mouse IgG:HRP (Rat Adsorbed)	STAR77	0.5 mg	C E P
Goat anti Mouse IgG/A/M:Alk. Phos.	STAR87A	1 mg	C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)	STAR87P	1 mg	E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800	STAR8D800GA	0.1 mg	F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC	STAR9B	1 mg	F

Negative Isotype Controls Available

Description	Product Code	Pack Size	Applications
Mouse IgG1 Negative Control:Alexa Fluor® 647	MCA1209A647	100 Tests/1ml	F
Mouse IgG1 Negative Control:Biotin	MCA1209B	0.1 mg	F
Mouse IgG1 Negative Control:FITC	MCA1209F	0.1 mg	F
Mouse IgG1 Negative Control	MCA1209	0.1 mg	F
Mouse IgG1 Negative Control:RPE	MCA1209PE	100 Tests	F

Application Based External Images

Flow Cytometry

Immunofluorescence

Immunohistology - Frozen

Immunohistology - Paraffin

Western Blotting

Product Specific References

References for MHC Class II RT1B antibody

McMaster, W.R. & Williams, A.F. (1979) Identification of Ia glycoproteins in rat thymus and purification from rat spleen.
Eur J Immunol. 9 (6): 426-33.
Fernandez, J.L. & Weeks, M. (1986) Genetic monitoring of inbred strains of mice using monoclonal antibodies to major histocompatibility haplotypes and lymphocyte alloantigens.
Lab Anim. 20 (4): 293-7.
Charteris, D.G. & Lightman, S.L. (1993) In vivo lymphokine production in experimental autoimmune uveoretinitis.
Immunology. 78 (3): 387-92.
Whiteland, J.L. et al. (1995) Immunohistochemical detection of T-cell subsets and other leukocytes in paraffin-embedded rat and mouse tissues with monoclonal antibodies.
J Histochem Cytochem. 43 (3): 313-20.
McKechnie, N.M. et al. (1997) Immunization with the cross-reactive antigens Ov39 from Onchocerca volvulus and hr44 from human retinal tissue induces ocular pathology and activates retinal microglia.
J Infect Dis. 176 (5): 1334-43.
Male, D.K. et al. (1987) Serological evidence for a defect in RT1.B (I-A) expression by the BDIX rat strain.
J Immunogenet. 14 (6): 301-12.
Burrows, G.G. et al. (1998) Two-domain MHC class II molecules form stable complexes with myelin basic protein 69-89 peptide that detect and inhibit rat encephalitogenic T cells and treat experimental autoimmune encephalomyelitis.
J Immunol. 161 (11): 5987-96.
Zilka, N. et al. (2009) Human misfolded truncated tau protein promotes activation of microglia and leukocyte infiltration in the transgenic rat model of tauopathy.
J Neuroimmunol. 209 (1-2): 16-25.
Kawamura, J. et al. (2010) Neuron-immune Interactions in the Sensitized Thalamus Induced by Mustard Oil Application to Rat Molar Pulp.
J Dent Res. 89: 1309-14.
Calvo, M. et al. (2010) Neuregulin-ErbB signaling promotes microglial proliferation and chemotaxis contributing to microgliosis and pain after peripheral nerve injury.
J Neurosci. 30 (15): 5437-50.
McClain, J.A. et al. (2011) Adolescent binge alcohol exposure induces long-lasting partial activation of microglia.
Brain Behav Immun. 25 Suppl 1: S120-8.
Baca Jones, C.C. et al. (2009) Rat cytomegalovirus infection depletes MHC II in bone marrow derived dendritic cells.
Virology. 388: 78-90.
Lobato-Pascual, A. et al. (2013) Rat macrophage C-type lectin is an activating receptor expressed by phagocytic cells.
PLoS One. 8: e57406.
Takizawa, T. et al. (2016) High-mobility group box 1 is an important mediator of microglial activation induced by cortical spreading depression
Journal of Cerebral Blood Flow & Metabolism. May 3 [Epub ahead of print]
Liu, M. et al. (2017) Pioglitazone Attenuates Neuroinflammation and Promotes Dopaminergic Neuronal Survival in the Nigrostriatal System of Rats after Diffuse Brain Injury.
J Neurotrauma. 34 (2): 414-22.
Noailles, A. et al. (2018) Systemic inflammation induced by lipopolysaccharide aggravates inherited retinal dystrophy.
Cell Death Dis. 9 (3): 350.

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