MHC Class II RT1B antibody | OX-6
Mouse anti Rat MHC Class II RT1B, clone OX-6 (MCA46R) used for the identification of MHC II expressing microglia in rat retinal sections by immunofluorescence.
Image caption:
Effect of LPS on retinal microglia in SD and P23H rats.
Representative vertical retinal sections immunostained with antibodies against Iba1 (green) and MHC-II (red) in vehicle- and LPS-injected SD rats a–f, or vehicle- and LPS-injected P23H rats g–l. Arrows point microglia MHC-II-immunopositive cells. Nuclei were stained with TO-PRO (blue). All images were taken in the central retina. GCL ganglion cell layer, IPL inner plexiform layer, OPL outer plexiform layer. Scale bar 40μm
From: Noailles A, Maneu V, Campello L, Lax P, Cuenca N.
Systemic inflammation induced by lipopolysaccharide aggravates inherited retinal dystrophy.
Cell Death Dis. 2018 Mar 2;9(3):350.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Rat MHC Class II RT1B antibody, clone OX-6 (MCA46G) used for the visualization of MHC class II expressing cells in rat spleen by immunohistochemistry on cryosections.
Image caption:
Immunostaining of rat spleen.
Serial-cut frozen sections were stained with mAbs towards A, rat MCL B, rat CD169 C, rat MHC class II D, human MHC class I (negative control) and visualized with peroxidase-conjugated secondary antibody and DAB substrate. RP: red pulp. PALS: periarteriolar lymphoid sheath. FOLL: follicle. MZ: marginal zone.
From: Lobato-Pascual A, Saether PC, Dahle MK, Gaustad P, Dissen E, Fossum S, et al. (2013)
Rat Macrophage C-Type Lectin Is an Activating Receptor Expressed by Phagocytic Cells.
PLoS ONE 8(2): e57406.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Rat MHC Class II RT1B antibody, clone OX-6 (MCA46G) used for the demonstration of MHC class II expressing cells by immunofluorescence.
Image caption:
Delayed recruitment of phagocytic cells and increased MHC II activation in the epineurium of DA rats.
Immunofluorescence micrographs illustrating sections of the whiskers area at 2 dpi (A, B, D, F, I and K) and 4 dpi (E, G, J and L). The DA (A, D, E, I and J) and the PVG (B, F, G, K and L) sections were stained with macrophage marker Iba1 (green, A, B, D–G and I–L), MHC II marker (red, A, B and D–G) to show the antigen presenting properties and phago-lysosome marker ED1 (red, I–L). Cellular quantification is represented in C, H and M panels. (A–C) At 2 dpi massive recruitment of Iba1+ cells (green) was present in the whiskers area of both DA (A) and PVG (B) rats at the site of the infection. However, the quantification of infiltrating Iba1+ cells to the epineurium (C) showed that the recruitment of macrophages was delayed and reduced in the epineurium of DA (arrow in A) compared to PVG rats (arrow in B). (D–H) At 2 dpi (D, F, H) the activation of antigen presenting cells detected by MHC II (red) was similar in both strains, but at 3 dpi (H) and 4 dpi (E and H) MHC II increased in DA compared to PVG rats (G and H). At 4 dpi in the whiskers area of DA rats (E) Iba1+/MHC II+ cells (arrows in E) were seen in the perineurium, in a pattern similar to the HSV-1 staining seen in Figure 1. In PVG rats (G), double and single stained Iba1+ and MHC II+ cells were more scattered in the vicinity of nerve fascicles (arrows in G), not delineating the perineurium in the same way as in DA. (I–M) Most Iba1+ cells were also ED1+ in both DA (I and J) and in PVG rats (K and L) indicating their phagocytic activity (arrows). However, there were more ED1+ cells in PVG at 2 dpi and 3 dpi compared to DA (M). At 4 dpi (J and L), Iba1+/ED1+ cells clearly delineated the perineurial cell layer in DA (arrows in J), but not in PVG (arrow in L), where these cells remained in the epineurium. Asterisks (E and G) point to the cellular infiltration into the epineurium; small arrows indicate cells in the perineurium; arrowheads indicate differences in Iba1+ morphology in DA compared to PVG rats; n, nerve fascicles; dots in panel C represent outliers. Scale bar: 50 μm.
From: Bereczky-Veress B, Abdelmagid N, Piehl F, Bergström T, Olsson T, Sköldenberg B, et al. (2010)
Influence of Perineurial Cells and Toll-Like Receptors 2 and 9 on Herpes simplex Type 1 Entry to the Central Nervous System in Rat Encephalitis.
PLoS ONE 5(8): e12350.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Rat MHC Class II RT1B antibody, clone OX-6 (MCA46PE) used for the evaluation of MHC Class II expression on rat adrenal cells by flow cytometry.
Image caption:
Intraadrenal MHCII+/CD86+ cells during collagen type II induced arthritis (CIA).
Quantification of MHCII and CD86 positive adrenal gland cells (exemplary FACS staining).
From: Stangl H, Krammetsvogl A, Lesiak M, Wolff C, Straub RH.
MHC/class-II-positive cells inhibit corticosterone of adrenal gland cells in experimental arthritis: a role for IL-1β, IL-18, and the inflammasome.
Sci Rep. 2020 Oct 13;10(1):17071.
doi: 10.1038/s41598-020-74309-0.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Rat MHC Class II RT1B:Alexa Fluor® 647
Mouse anti Rat MHC Class II RT1B:Biotin
Mouse anti Rat MHC Class II RT1B:FITC
Mouse anti Rat MHC Class II RT1B
Mouse anti Rat MHC Class II RT1B:RPE
- Product Type
- Monoclonal Antibody
- Clone
- OX-6
- Isotype
- IgG1
| Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|
| MCA46A647 | F | 100 Tests/1ml |
|
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| MCA46B | F | 0.1 mg |
|
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| MCA46FA | F | 50 µg |
|
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| MCA46FT | F | 0.1 mg |
|
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| MCA46GA | C F IF P* | 0.1 mg |
|
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| MCA46R | C F IF P* | 0.25 mg |
|
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| MCA46G | C F IF P* | 1 mg |
|
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| MCA46PE | F | 100 Tests |
|
Mouse anti Rat MHC Class II RT1B antibody, clone OX-6 recognizes a monomorphic determinant of the rat RT1B MHC class II antigen present on B lymphocytes, dendritic cells, some macrophages and certain epithelial cells.
Rat MHC Class II RT1B antibody, clone OX-6 does not react with the rat BDIX strain due to a defect in RT1B expression (Male et al. 1987).
The major histocompatibility complex (MHC) is a cluster of genes that are important in the immune response to infections. In rats, this complex is referred to as the RT1 region. In mice, this complex is referred to as the H-2 region.
Mouse anti Rat MHC Class II RT1B antibody, clone OX-6 also cross reacts with a polymorphic determinant on mouse strains of the H-2 haplotypes k and s. Analysis of recombinant mouse strains has mapped the OX-6 determinant to the H-2I-A region (McMaster and Williams 1979 and Male et al. 1987).
Mouse anti Rat MHC Class II RT1B antibody, clone OX-6 is routinely tested in flow cytometry on rat splenocytes.
Rat MHC Class II RT1B antibody, clone OX-6 does not react with the rat BDIX strain due to a defect in RT1B expression (Male et al. 1987).
The major histocompatibility complex (MHC) is a cluster of genes that are important in the immune response to infections. In rats, this complex is referred to as the RT1 region. In mice, this complex is referred to as the H-2 region.
Mouse anti Rat MHC Class II RT1B antibody, clone OX-6 also cross reacts with a polymorphic determinant on mouse strains of the H-2 haplotypes k and s. Analysis of recombinant mouse strains has mapped the OX-6 determinant to the H-2I-A region (McMaster and Williams 1979 and Male et al. 1987).
Mouse anti Rat MHC Class II RT1B antibody, clone OX-6 is routinely tested in flow cytometry on rat splenocytes.
Product Details
- Target Species
- Rat
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG conjugated to Alexa Fluor® 647 - liquid
- Product Form
- Purified IgG conjugated to Biotin - liquid
- Product Form
- Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
- Reconstitution
- Reconstitute with 1 ml distilled water
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin - Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin - Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin - Preservative Stabilisers
0.09% Sodium Azide - Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin 5% Sucrose - Carrier Free
- Yes
- Immunogen
- Rat thymocyte membrane glycoproteins.
- Approx. Protein Concentrations
- IgG concentration 0.05 mg/ml
- Approx. Protein Concentrations
- IgG concentration 0.1 mg/ml
- Approx. Protein Concentrations
- IgG concentration 0.1 mg/ml
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised BALB/c mice were fused with cells from the NS1 mouse myeloma cell line.
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Prior to reconstitution store at +4oC. Following reconstitution store at +4oC.
DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
More Information
- Acknowledgements
- This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
- Regulatory
- For research purposes only
Applications of MHC Class II RT1B antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry | Neat | 1/10 | |
| Flow Cytometry | Neat | 1/10 | |
| Flow Cytometry | Neat | 1/10 | |
| Flow Cytometry | 1/50 | 1/100 | |
| Immunofluorescence | |||
| Immunohistology - Frozen | |||
| Immunohistology - Paraffin 1 | 1/50 | 1/100 | |
| Flow Cytometry | Neat | 1/2 |
- 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose. PLP fixation is recommended for optimal results.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Copyright © 2020 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
Negative Isotype Controls Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Mouse IgG1 Negative Control:Alexa Fluor® 647 | MCA1209A647 | F | 100 Tests/1ml |
|
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| Mouse IgG1 Negative Control:Biotin | MCA1209B | F | 0.1 mg |
|
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| Mouse IgG1 Negative Control:FITC | MCA1209F | F | 0.1 mg |
|
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| Mouse IgG1 Negative Control | MCA1209 | F | 0.1 mg |
|
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| Mouse IgG1 Negative Control:RPE | MCA1209PE | F | 100 Tests |
|
Application Based External Images
Flow Cytometry
Immunofluorescence
Immunohistology - Paraffin
Western Blotting
Product Specific References
References for MHC Class II RT1B antibody
-
McMaster, W.R. & Williams, A.F. (1979) Identification of Ia glycoproteins in rat thymus and purification from rat spleen.
Eur J Immunol. 9 (6): 426-33. -
Fernandez, J.L. & Weeks, M. (1986) Genetic monitoring of inbred strains of mice using monoclonal antibodies to major histocompatibility haplotypes and lymphocyte alloantigens.
Lab Anim. 20 (4): 293-7. -
Charteris, D.G. & Lightman, S.L. (1993) In vivo lymphokine production in experimental autoimmune uveoretinitis.
Immunology. 78 (3): 387-92. -
Whiteland, J.L. et al. (1995) Immunohistochemical detection of T-cell subsets and other leukocytes in paraffin-embedded rat and mouse tissues with monoclonal antibodies.
J Histochem Cytochem. 43 (3): 313-20. -
McKechnie, N.M. et al. (1997) Immunization with the cross-reactive antigens Ov39 from Onchocerca volvulus and hr44 from human retinal tissue induces ocular pathology and activates retinal microglia.
J Infect Dis. 176 (5): 1334-43. -
Male, D.K. et al. (1987) Serological evidence for a defect in RT1.B (I-A) expression by the BDIX rat strain.
J Immunogenet. 14 (6): 301-12. -
Burrows, G.G. et al. (1998) Two-domain MHC class II molecules form stable complexes with myelin basic protein 69-89 peptide that detect and inhibit rat encephalitogenic T cells and treat experimental autoimmune encephalomyelitis.
J Immunol. 161 (11): 5987-96. -
Zilka, N. et al. (2009) Human misfolded truncated tau protein promotes activation of microglia and leukocyte infiltration in the transgenic rat model of tauopathy.
J Neuroimmunol. 209 (1-2): 16-25. -
Kawamura, J. et al. (2010) Neuron-immune Interactions in the Sensitized Thalamus Induced by Mustard Oil Application to Rat Molar Pulp.
J Dent Res. 89: 1309-14. -
Calvo, M. et al. (2010) Neuregulin-ErbB signaling promotes microglial proliferation and chemotaxis contributing to microgliosis and pain after peripheral nerve injury.
J Neurosci. 30 (15): 5437-50. -
McClain, J.A. et al. (2011) Adolescent binge alcohol exposure induces long-lasting partial activation of microglia.
Brain Behav Immun. 25 Suppl 1: S120-8. -
Baca Jones, C.C. et al. (2009) Rat cytomegalovirus infection depletes MHC II in bone marrow derived dendritic cells.
Virology. 388: 78-90. -
Lobato-Pascual, A. et al. (2013) Rat macrophage C-type lectin is an activating receptor expressed by phagocytic cells.
PLoS One. 8: e57406. -
Takizawa, T. et al. (2016) High-mobility group box 1 is an important mediator of microglial activation induced by cortical spreading depression
Journal of Cerebral Blood Flow & Metabolism. May 3 [Epub ahead of print] -
Liu, M. et al. (2017) Pioglitazone Attenuates Neuroinflammation and Promotes Dopaminergic Neuronal Survival in the Nigrostriatal System of Rats after Diffuse Brain Injury.
J Neurotrauma. 34 (2): 414-22. -
Noailles, A. et al. (2018) Systemic inflammation induced by lipopolysaccharide aggravates inherited retinal dystrophy.
Cell Death Dis. 9 (3): 350. -
Stangl, H. et al. (2020) MHC/class-II-positive cells inhibit corticosterone of adrenal gland cells in experimental arthritis: a role for IL-1β, IL-18, and the inflammasome.
Sci Rep. 10 (1): 17071.
