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IgA antibody | MARA-1

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Mouse anti Rat IgA Heavy Chain:Biotin

Mouse anti Rat IgA Heavy Chain:FITC

Mouse anti Rat IgA Heavy Chain

Product Type
Monoclonal Antibody
Clone
MARA-1
Isotype
IgG1
Product Code Applications Pack Size List Price Quantity
MCA191B
Datasheet
E
SDS
0.5 mg loader

MCA191F
Datasheet
F
SDS
0.5 mg loader

MCA191
Datasheet
C E IF
SDS
0.25 mg loader

Mouse anti Rat IgA Heavy Chain antibody, clone MARA-1 recognizes the alpha heavy chain of rat immunoglobulin. Mouse anti Rat IgA Heavy Chain antibody, clone MARA-1 shows no cross-reactivity with other rat immunoglobulin classes.

Product Details

Target Species
Rat
Product Form
Purified IgG conjugated to Biotin - liquid
Product Form
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.1%Sodium Azide
50%Glycerol
Preservative Stabilisers
0.1%Sodium Azide
50%Glycerol
Preservative Stabilisers
0.1% sodium azide
Immunogen
Purified IR1060 IgA rat myeloma protein.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Approx. Protein Concentrations
IgG concentration 1 mg/ml
Approx. Protein Concentrations
IgG concentration 1 mg/ml
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the mouse SP2/0 myeloma cell line.

Storage Information

Storage
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Storage
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Storage
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Guarantee
12 months from date of despatch
Guarantee
12 months from date of despatch
Guarantee
12 months from date of despatch

More Information

Regulatory
For research purposes only

Applications of IgA antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 500ng/ml
Flow Cytometry 5ug/ml
ELISA 5ug/ml as coating antibody
Immunofluorescence
Immunohistology - Frozen
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 50ul of the suggested working dilution to label 106 cells in 100ul.
ELISA
Mouse anti Rat IgA Heavy Chain antibody, clone MARA-1 may be used as a coating antibody in a sandwich ELISA in combination with Mouse anti Rat κ/λ antibody, clone MARK-1/MARL-15 (MCA1296P) as detection reagent and purified rat IgA (PRP01) as antigen..
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A E WB 0.5 mg loader
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA F IF 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA F WB 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA F IF WB 0.1 mg loader
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F F 0.5 mg loader
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P C E WB 0.5 mg loader
Goat anti Mouse IgG (Fc):FITC STAR120F C F 1 mg loader
Goat anti Mouse IgG (Fc):HRP STAR120P E WB 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A F 1 ml loader
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B C E P RE WB 1 mg loader
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 F 0.5 mg loader
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 F 1 ml loader
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 C E P 0.5 mg loader
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A C E WB 1 mg loader
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P E 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA F IF WB 0.1 mg loader
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B F 1 mg loader

Negative Isotype Controls Available

Description Product Code Applications Pack Size List Price Quantity
Mouse IgG1 Negative Control:FITC MCA1209F F 0.1 mg loader

Application Based External Images

Immunofluorescence

Immunohistology - Frozen

Product Specific References

References for IgA antibody

  1. Bjersing, J.L. et al. (2002) Loss of ileal IgA+ plasma cells and of CD4+ lymphocytes in ileal Peyer's patches of vitamin A deficient rats.
    Clin Exp Immunol. 130: 404-8.
  2. Budeč, M. et al. (2007) Possible mechanism of acute effect of ethanol on intestinal IgA expression in rat.
    Int Immunopharmacol. 7: 858-63.
  3. Budeč, M. et al. (2009) Blockade of nitric oxide synthesis modulates rat immunoglobulin A.
    Neuroimmunomodulation. 16: 155-61.
  4. Hahn, A. et al. (2010) Mesenteric lymph nodes are not required for an intestinal immunoglobulin A response to oral cholera toxin.
    Immunology. 129: 427-36.
  5. Herías, M.V. et al. (1999) Immunomodulatory effects of Lactobacillus plantarum colonizing the intestine of gnotobiotic rats
    Clin Exp Immunol. 116: 283-90.
  6. Ito, H. et al. (2011) Degree of polymerization of inulin-type fructans differentially affects number of lactic acid bacteria, intestinal immune functions, and immunoglobulin A secretion in the rat cecum.
    J Agric Food Chem. 59 (10): 5771-8.
  7. Kushnir, N. et al. (1998) Dendritic cells and resting B cells form clusters in vitro and in vivo: T cell independence, partial LFA-1 dependence, and regulation by cross-linking surface molecules.
    J Immunol. 160: 1774-81.
  8. Goodrich, M.E. and McGee, D.W. (1998) Regulation of mucosal B cell immunoglobulin secretion by intestinal epithelial cell-derived cytokines.
    Cytokine. 10: 948-55.
  9. Heel, K.A. et al. (1998) The effect of minimum luminal nutrition on mucosal cellularity and immunity of the gut.
    J Gastroenterol Hepatol. 13: 1015-9.
  10. Pérez-Cano FJ (2005) Neonatal immunoglobulin secretion and lymphocyte phenotype in rat small intestine lamina propria.
    Pediatr Res. 58: 164-9.
  11. Peng, Z. et al. (1996) Cross-reactivity and molecular mass of the epsilon chains of the IgE antibodies in dogs, humans, rats, and mice.
    Int Arch Allergy Immunol. 110: 149-55.
  12. Nayak, B.N. et al. (2009) Energy-restricted diets result in higher numbers of CD4+, CD8+, immunoglobulins (A, M, and G), and CD45RA cells in spleen and CD4+, immunoglobulin A, and CD45RA cells in colonic lamina propria of rats.
    Nutr Res. 2009 Jul;29(7):487-93.
  13. Komura, M. et al. (2014) A short-term ingestion of fructo-oligosaccharides increases immunoglobulin A and mucin concentrations in the rat cecum, but the effects are attenuated with the prolonged ingestion.
    Biosci Biotechnol Biochem. 78: 1592-602.
  14. Ito, H. et al. (2011) Degree of polymerization of inulin-type fructans differentially affects number of lactic acid bacteria, intestinal immune functions, and immunoglobulin A secretion in the rat cecum.
    J Agric Food Chem. 59: 5771-8.
  15. Hino, S. et al. (2020) Mucin-Derived O-Glycans Act as Endogenous Fiber and Sustain Mucosal Immune Homeostasis via Short-Chain Fatty Acid Production in Rat Cecum.
    J Nutr. 150 (10): 2656-65.

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