IgA antibody | MARA-1
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Mouse anti Rat IgA Heavy Chain antibody, clone MARA-1 recognizes the alpha heavy chain of rat immunoglobulin. Mouse anti Rat IgA Heavy Chain antibody, clone MARA-1 shows no cross-reactivity with other rat immunoglobulin classes.
Product Details
- Target Species
- Rat
- Product Form
- Purified IgG conjugated to Biotin - liquid
- Product Form
- Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.1% Sodium Azide 50% Glycerol - Preservative Stabilisers
0.1% Sodium Azide 50% Glycerol - Preservative Stabilisers
- 0.1% sodium azide
- Immunogen
- Purified IR1060 IgA rat myeloma protein.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Approx. Protein Concentrations
- IgG concentration 1 mg/ml
- Approx. Protein Concentrations
- IgG concentration 1 mg/ml
- Fusion Partners
- Spleen cells from immunised BALB/c mice were fused with cells of the mouse SP2/0 myeloma cell line.
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Shelf Life
- 18 months from date of despatch.
- Shelf Life
- 18 months from date of despatch.
- Shelf Life
- 18 months from date of despatch.
More Information
- Regulatory
- For research purposes only
Applications of IgA antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | 500ng/ml | ||
| Flow Cytometry | 5ug/ml | ||
| ELISA | 5ug/ml as coating antibody | ||
| Immunofluorescence | |||
| Immunohistology - Frozen |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 50ul of the suggested working dilution to label 106 cells in 100ul.
- ELISA
- Mouse anti Rat IgA Heavy Chain antibody, clone MARA-1 may be used as a coating antibody in a sandwich ELISA in combination with Mouse anti Rat κ/λ antibody, clone MARK-1/MARL-15 (MCA1296P) as detection reagent and purified rat IgA (PRP01) as antigen..
Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
Negative Isotype Controls Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Mouse IgG1 Negative Control:FITC | MCA1209F | 0.1 mg | F |
Product Specific References
References for IgA antibody
-
Bjersing, J.L. et al. (2002) Loss of ileal IgA+ plasma cells and of CD4+ lymphocytes in ileal Peyer's patches of vitamin A deficient rats.
Clin Exp Immunol. 130: 404-8. -
Budeč, M. et al. (2007) Possible mechanism of acute effect of ethanol on intestinal IgA expression in rat.
Int Immunopharmacol. 7: 858-63. -
Budeč, M. et al. (2009) Blockade of nitric oxide synthesis modulates rat immunoglobulin A.
Neuroimmunomodulation. 16: 155-61. -
Hahn, A. et al. (2010) Mesenteric lymph nodes are not required for an intestinal immunoglobulin A response to oral cholera toxin.
Immunology. 129: 427-36. -
Herías, M.V. et al. (1999) Immunomodulatory effects of Lactobacillus plantarum colonizing the intestine of gnotobiotic rats
Clin Exp Immunol. 116: 283-90. -
Ito, H. et al. (2011) Degree of polymerization of inulin-type fructans differentially affects number of lactic acid bacteria, intestinal immune functions, and immunoglobulin A secretion in the rat cecum.
J Agric Food Chem. 59 (10): 5771-8. -
Kushnir, N. et al. (1998) Dendritic cells and resting B cells form clusters in vitro and in vivo: T cell independence, partial LFA-1 dependence, and regulation by cross-linking surface molecules.
J Immunol. 160: 1774-81. -
Goodrich, M.E. and McGee, D.W. (1998) Regulation of mucosal B cell immunoglobulin secretion by intestinal epithelial cell-derived cytokines.
Cytokine. 10: 948-55. -
Heel, K.A. et al. (1998) The effect of minimum luminal nutrition on mucosal cellularity and immunity of the gut.
J Gastroenterol Hepatol. 13: 1015-9. -
Pérez-Cano FJ (2005) Neonatal immunoglobulin secretion and lymphocyte phenotype in rat small intestine lamina propria.
Pediatr Res. 58: 164-9. -
Peng, Z. et al. (1996) Cross-reactivity and molecular mass of the epsilon chains of the IgE antibodies in dogs, humans, rats, and mice.
Int Arch Allergy Immunol. 110: 149-55. -
Nayak, B.N. et al. (2009) Energy-restricted diets result in higher numbers of CD4+, CD8+, immunoglobulins (A, M, and G), and CD45RA cells in spleen and CD4+, immunoglobulin A, and CD45RA cells in colonic lamina propria of rats.
Nutr Res. 2009 Jul;29(7):487-93. -
Komura, M. et al. (2014) A short-term ingestion of fructo-oligosaccharides increases immunoglobulin A and mucin concentrations in the rat cecum, but the effects are attenuated with the prolonged ingestion.
Biosci Biotechnol Biochem. 78: 1592-602. -
Ito, H. et al. (2011) Degree of polymerization of inulin-type fructans differentially affects number of lactic acid bacteria, intestinal immune functions, and immunoglobulin A secretion in the rat cecum.
J Agric Food Chem. 59: 5771-8.
