IgA antibody | MARA-1
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Mouse anti Rat IgA Heavy Chain
- Product Type
- Monoclonal Antibody
- Clone
- MARA-1
- Isotype
- IgG1
- Specificity
- IgA
- Region
- HEAVY CHAIN
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| Mouse anti Rat IgA Heavy Chain antibody, clone MARA-1 recognizes the alpha heavy chain of rat immunoglobulin. Mouse anti Rat IgA Heavy Chain antibody, clone MARA-1 shows no cross-reactivity with other rat immunoglobulin classes. |
- Target Species
- Rat
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.1% sodium azide
- Immunogen
- Purified IR1060 IgA rat myeloma protein.
- Approx. Protein Concentrations
- IgG concentration 1 mg/ml
- Fusion Partners
- Spleen cells from immunised BALB/c mice were fused with cells of the mouse SP2/0 myeloma cell line.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
5ug/ml as coating antibody | |
| Immunofluorescence | |
||
| Immunohistology - Frozen | |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- ELISA
- Mouse anti Rat IgA Heavy Chain antibody, clone MARA-1 may be used as a coating antibody for the evaluation of rat IgA expression in a sandwich ELISA in combination with Mouse anti Rat κ/λ antibody, clone MARK-1/MARL-15 (MCA1296P) as detection reagent.
References for IgA antibody
-
Bjersing, J.L. et al. (2002) Loss of ileal IgA+ plasma cells and of CD4+ lymphocytes in ileal Peyer's patches of vitamin A deficient rats.
Clin Exp Immunol. 130: 404-8. -
Budeč, M. et al. (2007) Possible mechanism of acute effect of ethanol on intestinal IgA expression in rat.
Int Immunopharmacol. 7: 858-63. -
Budeč, M. et al. (2009) Blockade of nitric oxide synthesis modulates rat immunoglobulin A.
Neuroimmunomodulation. 16: 155-61. -
Hahn, A. et al. (2010) Mesenteric lymph nodes are not required for an intestinal immunoglobulin A response to oral cholera toxin.
Immunology. 129: 427-36. -
Herías, M.V. et al. (1999) Immunomodulatory effects of Lactobacillus plantarum colonizing the intestine of gnotobiotic rats
Clin Exp Immunol. 116: 283-90. -
Ito, H. et al. (2011) Degree of polymerization of inulin-type fructans differentially affects number of lactic acid bacteria, intestinal immune functions, and immunoglobulin A secretion in the rat cecum.
J Agric Food Chem. 59 (10): 5771-8. -
Kushnir, N. et al. (1998) Dendritic cells and resting B cells form clusters in vitro and in vivo: T cell independence, partial LFA-1 dependence, and regulation by cross-linking surface molecules.
J Immunol. 160: 1774-81. -
Goodrich, M.E. and McGee, D.W. (1998) Regulation of mucosal B cell immunoglobulin secretion by intestinal epithelial cell-derived cytokines.
Cytokine. 10: 948-55.
View The Latest Product References
-
Heel, K.A. et al. (1998) The effect of minimum luminal nutrition on mucosal cellularity and immunity of the gut.
J Gastroenterol Hepatol. 13: 1015-9. -
Pérez-Cano FJ (2005) Neonatal immunoglobulin secretion and lymphocyte phenotype in rat small intestine lamina propria.
Pediatr Res. 58: 164-9. -
Peng, Z. et al. (1996) Cross-reactivity and molecular mass of the epsilon chains of the IgE antibodies in dogs, humans, rats, and mice.
Int Arch Allergy Immunol. 110: 149-55. -
Nayak, B.N. et al. (2009) Energy-restricted diets result in higher numbers of CD4+, CD8+, immunoglobulins (A, M, and G), and CD45RA cells in spleen and CD4+, immunoglobulin A, and CD45RA cells in colonic lamina propria of rats.
Nutr Res. 2009 Jul;29(7):487-93. -
Komura, M. et al. (2014) A short-term ingestion of fructo-oligosaccharides increases immunoglobulin A and mucin concentrations in the rat cecum, but the effects are attenuated with the prolonged ingestion.
Biosci Biotechnol Biochem. 78: 1592-602. -
Ito, H. et al. (2011) Degree of polymerization of inulin-type fructans differentially affects number of lactic acid bacteria, intestinal immune functions, and immunoglobulin A secretion in the rat cecum.
J Agric Food Chem. 59: 5771-8. -
Hino, S. et al. (2020) Mucin-Derived O-Glycans Act as Endogenous Fiber and Sustain Mucosal Immune Homeostasis via Short-Chain Fatty Acid Production in Rat Cecum.
J Nutr. 150 (10): 2656-65. -
Tulinská, J. et al. (2018) Humoral and cellular immune response in Wistar Han RCC rats fed two genetically modified maize MON810 varieties for 90 days (EU 7th Framework Programme project GRACE).
Arch Toxicol. 92 (7): 2385-99.
- RRID
- AB_322196
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