CD90 antibody | OX-7

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MCA47G
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C F IF IP WB 1 mg loader
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MCA47GA
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C F IF IP 0.1 mg loader
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MCA47R
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C F IF IP WB 0.25 mg loader
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MCA47RT
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C F IF IP WB 25 µg loader
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Mouse anti Rat CD90 antibody, clone OX-7 recognizes rat and CD90, also known as Thy1.1, a GPI-anchored membrane protein containing a single V type Ig-like domain CD90 is expressed on a variety of cell types including thymocytes, neuronal cells, stem cells, immature B cells and connective tissues, CD90 is also expressed in T cells in mice.

Since Thy1.1 is a monomorphic determinant in rat but polymorphic in mice, clone MRC OX-7 reacts with Thy1.1 mice e.g. AKR and FVB, but not Thy1.2 mice such as CBA and BALB/c. The affinity of the Fab′ of MRC OX-7 for rat Thy1 is 3 x 109m-1 and for mouse Thy1.1 is 3 x 108m-1(1).

Mouse anti rat CD90, clone MRC OX-7 has been demonstrated to promote neurite outgrowths on peripherin-stained sympathetic rat neurons, using fluorescence microscopy (Jeng et al. 1998). Clone OX-7 has also been reported to induce glomerular nephritis in Wistar rats (Tamura et al. 1996).

This product is routinely tested in flow cytometry on rat thymocytes.

Target Species
Rat
Species Cross-Reactivity
Target SpeciesCross Reactivity
Rabbit
Mouse
Guinea Pig
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Carrier Free
Yes
Immunogen
Rat Thy1 antigen.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS1 myeloma cell line.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/50 1/100
Immunofluorescence
Immunohistology - Frozen
Immunoprecipitation
Western Blotting
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.

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Description Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)
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Description Goat anti Mouse IgG (Fc):FITC
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Description Goat anti Mouse IgG (Fc):HRP
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Description Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):DyLight®650 (Multi Species Adsorbed) STAR117D650 F IF 0.1 mg loader
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Description Goat anti Mouse IgG (H/L):DyLight®650 (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):DyLight®550 (Multi Species Adsorbed) STAR117D550 F IF WB 0.1 mg loader
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Description Goat anti Mouse IgG (H/L):DyLight®550 (Multi Species Adsorbed)

Description Product Code Applications Pack Size List Price Your Price Quantity
Mouse IgG1 Negative Control MCA1209 F 0.1 mg loader
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Description Mouse IgG1 Negative Control

References for CD90 antibody

  1. Mason, D.W. & Williams, A.F. (1980) The kinetics of antibody binding to membrane antigens in solution and at the cell surface.
    Biochem J. 187 (1): 1-20.
  2. Campbell, D.G. et al. (1981) Rat brain Thy-1 glycoprotein. The amino acid sequence, disulphide bonds and an unusual hydrophobic region.
    Biochem J. 195 (1): 15-30.
  3. Bukovský, A. et al. (1983) The localization of Thy-1.1, MRC OX 2 and Ia antigens in the rat ovary and fallopian tube.
    Immunology. 48 (3): 587-96.
  4. Lee, W.S. et al. (1998) Thy-1, a novel marker for angiogenesis upregulated by inflammatory cytokines.
    Circ Res. 82 (8): 845-51.
  5. Jeng, C.J. et al. (1998) Thy-1 is a component common to multiple populations of synaptic vesicles.
    J Cell Biol. 140 (3): 685-98.
  6. Banerjee, S.A. et al. (1997) An antibody to the tetraspan membrane protein CD9 promotes neurite formation in a partially alpha3beta1 integrin-dependent manner.
    J Neurosci. 17 (8): 2756-65.
  7. Kawachi, H. et al. (1992) Epitope-specific induction of mesangial lesions with proteinuria by a MoAb against mesangial cell surface antigen.
    Clin Exp Immunol. 88 (3): 399-404.
  8. Tamura, M. et al. (1996) Enhanced glomerular profilin gene and protein expression in experimental mesangial proliferative glomerulonephritis.
    Biochem Biophys Res Commun. 222 (3): 683-7.
  9. View The Latest Product References
  10. Stevenson, K.S. et al. (2009) Isolation, characterization, and differentiation of thy1.1-sorted pancreatic adult progenitor cell populations.
    Stem Cells Dev. 18 (10): 1389-98.
  11. Biermann, J. et al. (2011) Histone deacetylase inhibitors sodium butyrate and valproic acid delay spontaneous cell death in purified rat retinal ganglion cells.
    Mol Vis. 17: 395-403.
  12. Keller, R.K. et al. (2004) Formation of 7-dehydrocholesterol-containing membrane rafts in vitro and in vivo, with relevance to the Smith-Lemli-Opitz syndrome.
    J Lipid Res. 45: 347-55.
  13. Ohashi, N. et al. (2010) Glomerular angiotensinogen is induced in mesangial cells in diabetic rats via reactive oxygen species--ERK/JNK pathways.
    Hypertens Res. 33:1174-81.
  14. Maia L et al. (2016) Conditioned medium: A new alternative for cryopreservation of equine umbilical cord mesenchymal stem cells.
    Cell Biol Int. Nov 26. [Epub ahead of print]
  15. Rutigliano, J.A. et al. (2008) Screening monoclonal antibodies for cross-reactivity in the ferret model of influenza infection.
    J Immunol Methods. 336: 71-7.
  16. Freisinger, W. et al. (2013) Sensory renal innervation: a kidney-specific firing activity due to a unique expression pattern of voltage-gated sodium channels?
    Am J Physiol Renal Physiol. 304: F491-7.
  17. Shimizu T et al. (2016) Bioactivity of sol-gel-derived TiO2 coating on polyetheretherketone: In vitro and in vivo studies.
    Acta Biomater. 35: 305-17.
  18. Maia, L. et al. (2017) A proteomic study of mesenchymal stem cells from equine umbilical cord.
    Theriogenology. 100: 8-15.
  19. Chang, J.C. et al. (2019) Early Immune Response to Acute Gastric Fluid Aspiration in a Rat Model of Lung Transplantation.
    Exp Clin Transplant. 17 (1): 84-92.
  20. Huang, X. et al. (2019) MRI Tracking of SPIO- and Fth1-Labeled Bone Marrow Mesenchymal Stromal Cell Transplantation for Treatment of Stroke.
    Contrast Media Mol Imaging. 2019: 5184105.
  21. Zhao, Y. et al. (2017) A new electrospun graphene-silk fibroin composite scaffolds for guiding Schwann cells.
    J Biomater Sci Polym Ed. 28 (18): 2171-85.
  22. Cąkała-Jakimowicz, M. & Puzianowska-Kuznicka, M. (2022) Towards Understanding the Lymph Node Response to Skin Infection with Saprophytic Staphylococcus epidermidis..
    Biomedicines. 10 (5): 1021.
  23. Huang, S. et al. (2022) Hydrogen sulfide supplement preserves mitochondrial function of retinal ganglion cell in a rat glaucoma model.
    Cell Tissue Res. May 20 [Epub ahead of print].
  24. Cacciamali, A. et al. (2022) Engineered nanoparticles toxicity on adipose tissue derived mesenchymal stem cells: A preliminary investigation
    Res Vet Sci. 8 Aug [Epub ahead of print].
  25. Eweida, A. et al. (2022) Systemically injected bone marrow mononuclear cells specifically home to axially vascularized tissue engineering constructs.
    PLoS One. 17 (8): e0272697.

Flow Cytometry

Immunofluorescence

Immunoprecipitation

Western Blotting

Synonyms
THY1
RRID
AB_321889
UniProt
P01830
Entrez Gene
Thy1
GO Terms
GO:0001525 angiogenesis
GO:0007010 cytoskeleton organization
GO:0005829 cytosol
GO:0005100 Rho GTPase activator activity
GO:0006469 negative regulation of protein kinase activity
GO:0016337 cell-cell adhesion
GO:0019901 protein kinase binding
GO:0030336 negative regulation of cell migration
GO:0030426 growth cone
GO:0034235 GPI anchor binding
GO:0043066 negative regulation of apoptosis
GO:0043547 positive regulation of GTPase activity
GO:0045576 mast cell activation
GO:0046549 retinal cone cell development
GO:0048041 focal adhesion assembly
GO:0048147 negative regulation of fibroblast proliferation
GO:0050731 positive regulation of peptidyl-tyrosine phosphorylation
GO:0050771 negative regulation of axonogenesis
GO:0050852 T cell receptor signaling pathway
GO:0050860 negative regulation of T cell receptor signaling pathway
GO:0050870 positive regulation of T cell activation
GO:0051281 positive regulation of release of sequestered calcium ion into cytosol
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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