CD90 antibody | OX-7
Mouse anti Rat CD90 antibody, clone OX-7 (MCA47R) used to label THY-1 expressing cells in rat lymph nodes by immunohistochemistry on cryostat sections.
Image caption:
Paracortex of the rat popliteal lymph node in initial massive S. epidermidis infection (days 1–7) with early node’s evaluation (day 8) stained for the presence of large stem cells, thymocytes and immature B cells (CD90+, red). Magnification 1000×.
From: Cąkała-Jakimowicz, M.; Puzianowska-Kuznicka, M.
Towards Understanding the Lymph Node Response to Skin Infection with Saprophytic Staphylococcus epidermidis.
Biomedicines 2022, 10, 1021.
doi: 10.3390/biomedicines10051021
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Rat CD90 antibody, clone OX-7 (MCA47R) used to label THY-1 expressing cells in rat lymph nodes by immunohistochemistry on cryostat sections.
Image caption:
The medulla of the rat popliteal lymph node in initial massive S. epidermidis infection (days 1–7) with early node’s evaluation (day 8). The node stained for: (A) Dendritic cells (OX62+, red). (B) Macrophages and monocytes (CD68+, red). (C) Stem cells, thymocytes and immature B cells (CD90+, red). (D) T helper lymphocytes and monocytes (CD4+, red). (E) Activated antigen-presenting cells (MHC class II+, red). Magnification 1000×.
From: Cąkała-Jakimowicz, M.; Puzianowska-Kuznicka, M.
Towards Understanding the Lymph Node Response to Skin Infection with Saprophytic Staphylococcus epidermidis.
Biomedicines 2022, 10, 1021.
doi: 10.3390/biomedicines10051021
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Rat CD90 antibody, clone OX-7 (MCA47R) used to sort THY-1 expressing cells from rat liver by flow cytometry.
Image caption:
Transplantation of Thy1-LSPCs and Thy1-MCs to Ret/PH-treated rat livers.
(A) Schematic showing the cell transplantation method.
From: Ichinohe, N. et al.
CINC-2 and miR-199a-5p in exosomes secreted by transplanted Thy1+ cells activate hepatocytic progenitor cell growth in rat liver regeneration.
Res Sq. 20 Jan [Preprint].
doi: 10.21203/rs.3.rs-2087658/v1
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Rat CD90
- Product Type
- Monoclonal Antibody
- Clone
- OX-7
- Isotype
- IgG1
- Specificity
- CD90
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| Mouse anti Rat CD90 antibody, clone OX-7 recognizes rat and CD90, also known as Thy1.1, a GPI-anchored membrane protein containing a single V type Ig-like domain CD90 is expressed on a variety of cell types including thymocytes, neuronal cells, stem cells, immature B cells and connective tissues, CD90 is also expressed in T cells in mice. Since Thy1.1 is a monomorphic determinant in rat but polymorphic in mice, clone MRC OX-7 reacts with Thy1.1 mice e.g. AKR and FVB, but not Thy1.2 mice such as CBA and BALB/c. The affinity of the Fab′ of MRC OX-7 for rat Thy1 is 3 x 109m-1 and for mouse Thy1.1 is 3 x 108m-1(1). Mouse anti rat CD90, clone MRC OX-7 has been demonstrated to promote neurite outgrowths on peripherin-stained sympathetic rat neurons, using fluorescence microscopy (Jeng et al. 1998). Clone OX-7 has also been reported to induce glomerular nephritis in Wistar rats (Tamura et al. 1996). This product is routinely tested in flow cytometry on rat thymocytes. |
- Target Species
- Rat
- Species Cross-Reactivity
-
Target Species Cross Reactivity Rabbit Mouse Guinea Pig - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide - Carrier Free
- Yes
- Immunogen
- Rat Thy1 antigen.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunized BALB/c mice were fused with cells of the mouse NS1 myeloma cell line.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
1/50 | 1/100 |
| Immunofluorescence | |
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| Immunohistology - Frozen | |
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| Immunoprecipitation | |
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| Western Blotting | |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
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| Mouse IgG1 Negative Control | MCA1209 | F | 0.1 mg |
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| Description | Mouse IgG1 Negative Control | ||||||
Source Reference
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Mason, D.W. & Williams, A.F. (1980) The kinetics of antibody binding to membrane antigens in solution and at the cell surface.
Biochem J. 187 (1): 1-20.
References for CD90 antibody
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Campbell, D.G. et al. (1981) Rat brain Thy-1 glycoprotein. The amino acid sequence, disulphide bonds and an unusual hydrophobic region.
Biochem J. 195 (1): 15-30. -
Bukovský, A. et al. (1983) The localization of Thy-1.1, MRC OX 2 and Ia antigens in the rat ovary and fallopian tube.
Immunology. 48 (3): 587-96. -
Kawachi, H. et al. (1992) Epitope-specific induction of mesangial lesions with proteinuria by a MoAb against mesangial cell surface antigen.
Clin Exp Immunol. 88 (3): 399-404. -
Tamura, M. et al. (1996) Enhanced glomerular profilin gene and protein expression in experimental mesangial proliferative glomerulonephritis.
Biochem Biophys Res Commun. 222 (3): 683-7. -
Banerjee, S.A. et al. (1997) An antibody to the tetraspan membrane protein CD9 promotes neurite formation in a partially alpha3beta1 integrin-dependent manner.
J Neurosci. 17 (8): 2756-65. -
Lee, W.S. et al. (1998) Thy-1, a novel marker for angiogenesis upregulated by inflammatory cytokines.
Circ Res. 82 (8): 845-51. -
Keller, R.K. et al. (2004) Formation of 7-dehydrocholesterol-containing membrane rafts in vitro and in vivo, with relevance to the Smith-Lemli-Opitz syndrome.
J Lipid Res. 45: 347-55. -
Rutigliano, J.A. et al. (2008) Screening monoclonal antibodies for cross-reactivity in the ferret model of influenza infection.
J Immunol Methods. 336: 71-7.
View The Latest Product References
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Stevenson, K.S. et al. (2009) Isolation, characterization, and differentiation of thy1.1-sorted pancreatic adult progenitor cell populations.
Stem Cells Dev. 18 (10): 1389-98. -
Ohashi, N. et al. (2010) Glomerular angiotensinogen is induced in mesangial cells in diabetic rats via reactive oxygen species--ERK/JNK pathways.
Hypertens Res. 33:1174-81. -
Biermann, J. et al. (2011) Histone deacetylase inhibitors sodium butyrate and valproic acid delay spontaneous cell death in purified rat retinal ganglion cells.
Mol Vis. 17: 395-403. -
Freisinger, W. et al. (2013) Sensory renal innervation: a kidney-specific firing activity due to a unique expression pattern of voltage-gated sodium channels?
Am J Physiol Renal Physiol. 304: F491-7. -
Shimizu T et al. (2016) Bioactivity of sol-gel-derived TiO2 coating on polyetheretherketone: In vitro and in vivo studies.
Acta Biomater. 35: 305-17. -
Maia, L. et al. (2017) Conditioned medium: a new alternative for cryopreservation of equine umbilical cord mesenchymal stem cells.
Cell Biol Int. 41 (3): 239-48. -
Maia, L. et al. (2017) A proteomic study of mesenchymal stem cells from equine umbilical cord.
Theriogenology. 100: 8-15. -
Zhao, Y. et al. (2017) A new electrospun graphene-silk fibroin composite scaffolds for guiding Schwann cells.
J Biomater Sci Polym Ed. 28 (18): 2171-85. -
Chang, J.C. et al. (2019) Early Immune Response to Acute Gastric Fluid Aspiration in a Rat Model of Lung Transplantation.
Exp Clin Transplant. 17 (1): 84-92. -
Huang, X. et al. (2019) MRI Tracking of SPIO- and Fth1-Labeled Bone Marrow Mesenchymal Stromal Cell Transplantation for Treatment of Stroke.
Contrast Media Mol Imaging. 2019: 5184105. -
Kuriyama, T. et al. (2020) A novel rat model of inflammatory bowel disease developed using a device created with a 3D printer.
Regen Ther. 14: 1-10. -
Cąkała-Jakimowicz, M. & Puzianowska-Kuznicka, M. (2022) Towards Understanding the Lymph Node Response to Skin Infection with Saprophytic Staphylococcus epidermidis..
Biomedicines. 10 (5): 1021. -
Huang, S. et al. (2022) Hydrogen sulfide supplement preserves mitochondrial function of retinal ganglion cell in a rat glaucoma model.
Cell Tissue Res. 389 (2): 171-85. -
Cacciamali, A. et al. (2022) Engineered nanoparticles toxicity on adipose tissue derived mesenchymal stem cells: A preliminary investigation
Res Vet Sci. 152: 134-49. -
Eweida, A. et al. (2022) Systemically injected bone marrow mononuclear cells specifically home to axially vascularized tissue engineering constructs.
PLoS One. 17 (8): e0272697. -
Ichinohe, N. et al. (2023) CINC-2 and miR-199a-5p in EVs secreted by transplanted Thy1(+) cells activate hepatocytic progenitor cell growth in rat liver regeneration.
Stem Cell Res Ther. 14 (1): 134. -
Numata-Uematasu, Y. et al. (2023) In vitro myelination using explant culture of dorsal root ganglia: An efficient tool for analyzing peripheral nerve differentiation and disease modeling.
PLoS One. 18 (5): e0285897.
- Synonyms
- THY1
- RRID
- AB_321889
- UniProt
- P01830
- Entrez Gene
- Thy1
- GO Terms
- GO:0001525 angiogenesis
- GO:0007010 cytoskeleton organization
- GO:0005829 cytosol
- GO:0005100 Rho GTPase activator activity
- GO:0006469 negative regulation of protein kinase activity
- GO:0016337 cell-cell adhesion
- GO:0019901 protein kinase binding
- GO:0030336 negative regulation of cell migration
- GO:0030426 growth cone
- View More GO Terms
- GO:0034235 GPI anchor binding
- GO:0043066 negative regulation of apoptosis
- GO:0043547 positive regulation of GTPase activity
- GO:0045576 mast cell activation
- GO:0046549 retinal cone cell development
- GO:0048041 focal adhesion assembly
- GO:0048147 negative regulation of fibroblast proliferation
- GO:0050731 positive regulation of peptidyl-tyrosine phosphorylation
- GO:0050771 negative regulation of axonogenesis
- GO:0050852 T cell receptor signaling pathway
- GO:0050860 negative regulation of T cell receptor signaling pathway
- GO:0050870 positive regulation of T cell activation
- GO:0051281 positive regulation of release of sequestered calcium ion into cytosol
View more products with CD90 specificity
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