CD8 Alpha antibody | OX-8

Mouse anti Rat CD8 Alpha

Product Type
Monoclonal Antibody
Clone
OX-8
Isotype
IgG1
Specificity
CD8 Alpha

Product Code Applications Pack Size List Price Your Price Qty
MCA48R
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C F IF IP P WB 0.25 mg loader
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loader
MCA48G
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C F IF IP P WB 1 mg loader
List Price Your Price
loader
MCA48GA
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C F IP P 0.1 mg loader
List Price Your Price
loader
Search for Batch Specific Datasheets

Mouse anti Rat CD8α, clone MRC OX-8, recognizes the rat CD8 alpha cell surface antigen, expressed by a subset of T lymphocytes, most thymocytes and the majority of NK cells.

Mouse anti Rat CD8α, clone MRC OX-8 is suitable for use in in vitro blocking assays (Popov et al.2001).

Mouse anti Rat CD8α, clone MRC OX-8 reacts with paraffin-embedded material following PLP Fixation (periodate-lysine paraformaldehyde) (Whiteland et al. 1995).

Mouse anti Rat CD8α, clone MRC OX-8 is routinely tested in flow cytometry on rat splenocytes.

Target Species
Rat
Product Form
Purified IgG - liquid
Preparation
MCA48R, MCA48GA: Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant.
MCA48G: Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Carrier Free
Yes
Immunogen
Rat thymocyte membrane glycoproteins.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS1 myeloma cell line.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/50 1/100
Immunofluorescence
Immunohistology - Frozen
Immunohistology - Paraffin
Immunoprecipitation
Western Blotting
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Immunohistology
This product does not require protein digestion pre-treatment of paraffin embedded sections.
This product does not require antigen retrieval using heat treatment prior to staining of paraffin embedded sections.

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Description Mouse IgG1 Negative Control

References for CD8 Alpha antibody

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    Immunology. 123: 480-90.
  2. Bradl, M. et al. (2005) Complementary contribution of CD4 and CD8 T lymphocytes to T-cell infiltration of the intact and the degenerative spinal cord.
    Am J Pathol. 166: 1441-50.
  3. Hashimoto, Y. et al. (2003) Two major histocompatibility complex class I-restricted epitopes of the Borna disease virus p10 protein identified by cytotoxic T lymphocytes induced by DNA-based immunization.
    J Virol. 77: 6076-81.
  4. King, G.D. et al. (2008) Flt3L in combination with HSV1-TK-mediated gene therapy reverses brain tumor-induced behavioral deficits.
    Mol Ther. 16: 682-90
  5. Latta, M. et al. (2007) CXCR6 is expressed on T cells in both T helper type 1 (Th1) inflammation and allergen-induced Th2 lung inflammation but is only a weak mediator of chemotaxis.
    Immunology. 121: 555-64
  6. Brideau, R.J. et al. (1980) Two subsets of rat T lymphocytes defined with monoclonal antibodies.
    Eur J Immunol. 10 (8): 609-15.
  7. Lyscom, N. & Brueton, M.J. (1982) Intraepithelial, lamina propria and Peyer's patch lymphocytes of the rat small intestine: isolation and characterization in terms of immunoglobulin markers and receptors for monoclonal antibodies.
    Immunology. 45 (4): 775-83.
  8. Whiteland, J.L. et al. (1995) Immunohistochemical detection of T-cell subsets and other leukocytes in paraffin-embedded rat and mouse tissues with monoclonal antibodies.
    J Histochem Cytochem. 43 (3): 313-20.
  9. View The Latest Product References
  10. Bukovský A et al. (1984) Association of some cell surface antigens of lymphoid cells and cell surface differentiation antigens with early rat pregnancy.
    Immunology. 52 (4): 631-40.
  11. Popov, I. et al. (2001) The effect of an anti-HLA-B27 immune response on CTL recognition of Chlamydia.
    J Immunol. 167 (6): 3375-82.
  12. Mitnacht, R. et al. (1998) Opposite CD4/CD8 lineage decisions of CD4+8+ mouse and rat thymocytes to equivalent triggering signals: correlation with thymic expression of a truncated CD8 alpha chain in mice but not rats.
    J Immunol. 160 (2): 700-7.
  13. Thomas, M.L. & Green, J.R. (1983) Molecular nature of the W3/25 and MRC OX-8 marker antigens for rat T lymphocytes: comparisons with mouse and human antigens.
    Eur J Immunol. 13 (10): 855-8.
  14. Torres-Nagel, N. et al. (1992) Differential thymus dependence of rat CD8 isoform expression.
    Eur J Immunol. 22 (11): 2841-8.
  15. Zilka, N. et al. (2009) Human misfolded truncated tau protein promotes activation of microglia and leukocyte infiltration in the transgenic rat model of tauopathy.
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  17. Banerjee, S. et al. (2003) Development of organised conjunctival leucocyte aggregates after corneal transplantation in rats.
    Br J Ophthalmol. 87: 1515-22.
  18. Sanchez-Guajardo, V. (2010) Microglia acquire distinct activation profiles depending on the degree of alpha-synuclein neuropathology in a rAAV based model of Parkinson's disease.
    PLoS One. 5: e8784.
  19. Maenz, M. et al. (2011) A comprehensive flow-cytometric analysis of graft infiltrating lymphocytes, draining lymph nodes and serum during the rejection phase in a fully allogeneic rat cornea transplant model.
    Mol Vis. 2011 Feb 8;17:420-9.
  20. Granados-Durán P et al. (2015) Neuroinflammation induced by intracerebroventricular injection of microbial neuraminidase.
    Front Med (Lausanne). 2: 14.
  21. Nuccitelli R et al. (2015) Nanoelectroablation of Murine Tumors Triggers a CD8-Dependent Inhibition of Secondary Tumor Growth.
    PLoS One. 10 (7): e0134364.
  22. Zhang, Z.M. et al. (2016) Lesional accumulation of CD8(+) cells in sciatic nerves of experimental autoimmune neuritis rats.
    Neurol Sci. 37 (2): 199-203.
  23. Pamukcu, O. et al. (2016) Anti-inflammatory role of obestatin in autoimmune myocarditis.
    Clin Exp Pharmacol Physiol. 43 (1): 47-55.
  24. Dabrowska, S. et al. (2019) Human bone marrow mesenchymal stem cell-derived extracellular vesicles attenuate neuroinflammation evoked by focal brain injury in rats.
    J Neuroinflammation. 16 (1): 216.
  25. Arndt, T. et al. (2013) A variable CD3⁺ T-cell frequency in peripheral blood lymphocytes associated with type 1 diabetes mellitus development in the LEW.1AR1-iddm rat.
    PLoS One. 8 (5): e64305.
  26. James, R.E. et al. (2020) Persistent elevation of intrathecal pro-inflammatory cytokines leads to multiple sclerosis-like cortical demyelination and neurodegeneration.
    Acta Neuropathol Commun. 8 (1): 66.
  27. Matsuyama, S. et al. (2021) Properties of macrophages and lymphocytes appearing in rat renal fibrosis followed by repeated injection of cisplatin.
    J Vet Med Sci. 83 (9): 1435-42.
  28. Dabrowska, S. et al. (2021) Neuroinflammation evoked by brain injury in a rat model of lacunar infarct.
    Exp Neurol. 336: 113531.
  29. Schmiedl, A. et al. (2021) Lung development and immune status under chronic LPS exposure in rat pups with and without CD26/DPP4 deficiency.
    Cell Tissue Res. Oct 04 [Epub ahead of print].
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    Hum Reprod Open. 2021 (2): hoab012.
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    Mov Disord. Nov 12 [Epub ahead of print].
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    Multiple Sclerosis and Related Disorders. : 02 Nov: 103346.
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    J King Saud Uni - Science. 34 (3): 101886.
  34. Hoff, U. et al. (2022) The mTOR inhibitor Rapamycin protects from premature cellular senescence early after experimental kidney transplantation.
    PLoS One. 17 (4): e0266319.
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    Biomedicines. 10 (5): 1021.

Flow Cytometry

Immunofluorescence

Immunohistology - Frozen

Immunohistology - Paraffin

RRID
AB_321476
UniProt
P07725
Entrez Gene
Cd8a
GO Terms
GO:0016021 integral to membrane
GO:0006954 inflammatory response
GO:0019901 protein kinase binding
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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