CD8 Alpha antibody | OX-8
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|Mouse anti Rat CD8α, clone MRC OX-8, recognizes the rat CD8 alpha cell surface antigen, expressed by a subset of T lymphocytes, most thymocytes and the majority of NK cells.
Mouse anti Rat CD8α, clone MRC OX-8 is suitable for use in in vitro blocking assays (Popov et al.2001).
Mouse anti Rat CD8α, clone MRC OX-8 reacts with paraffin-embedded material following PLP Fixation (periodate-lysine paraformaldehyde) (Whiteland et al. 1995).
Mouse anti Rat CD8α, clone MRC OX-8 is routinely tested in flow cytometry on rat splenocytes.
- Target Species
- Product Form
- Purified IgG - liquid
- MCA48R, MCA48GA: Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant.
- MCA48G: Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide
- Carrier Free
- Rat thymocyte membrane glycoproteins.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS1 myeloma cell line.
- For research purposes only
- 12 months from date of despatch
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
|Application Name||Verified||Min Dilution||Max Dilution|
|Immunohistology - Frozen|
|Immunohistology - Paraffin|
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
- This product does not require protein digestion pre-treatment of paraffin embedded sections.
This product does not require antigen retrieval using heat treatment prior to staining of paraffin embedded sections.
References for CD8 Alpha antibody
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Bradl, M. et al. (2005) Complementary contribution of CD4 and CD8 T lymphocytes to T-cell infiltration of the intact and the degenerative spinal cord.
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Latta, M. et al. (2007) CXCR6 is expressed on T cells in both T helper type 1 (Th1) inflammation and allergen-induced Th2 lung inflammation but is only a weak mediator of chemotaxis.
Immunology. 121: 555-64
Brideau, R.J. et al. (1980) Two subsets of rat T lymphocytes defined with monoclonal antibodies.
Eur J Immunol. 10 (8): 609-15.
Lyscom, N. & Brueton, M.J. (1982) Intraepithelial, lamina propria and Peyer's patch lymphocytes of the rat small intestine: isolation and characterization in terms of immunoglobulin markers and receptors for monoclonal antibodies.
Immunology. 45 (4): 775-83.
Whiteland, J.L. et al. (1995) Immunohistochemical detection of T-cell subsets and other leukocytes in paraffin-embedded rat and mouse tissues with monoclonal antibodies.
J Histochem Cytochem. 43 (3): 313-20.
View The Latest Product References
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Popov, I. et al. (2001) The effect of an anti-HLA-B27 immune response on CTL recognition of Chlamydia.
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Mitnacht, R. et al. (1998) Opposite CD4/CD8 lineage decisions of CD4+8+ mouse and rat thymocytes to equivalent triggering signals: correlation with thymic expression of a truncated CD8 alpha chain in mice but not rats.
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Thomas, M.L. & Green, J.R. (1983) Molecular nature of the W3/25 and MRC OX-8 marker antigens for rat T lymphocytes: comparisons with mouse and human antigens.
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Torres-Nagel, N. et al. (1992) Differential thymus dependence of rat CD8 isoform expression.
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