CD54 antibody | 1A29

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Mouse anti Rat CD54:Alexa Fluor® 647

Mouse anti Rat CD54:FITC

Mouse anti Rat CD54:Low Endotoxin

Mouse anti Rat CD54

Product Type
Monoclonal Antibody
Clone
1A29
Isotype
IgG1
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA773A647 F 100 Tests/1ml
MCA773F F 0.1 mg
MCA773FB F 0.5 mg
MCA773EL C F FN IF IP P* WB 0.5 mg
MCA773GA C F IF IP P* 0.1 mg
MCA773 C F IP P* WB 0.2 mg
Mouse anti Rat CD54 antibody, clone 1A29 recognizes the rat CD54 cell surface antigen, also known as intercellular adhesion molecule-1 (ICAM-1), a ~90 kDa adhesion molecule belonging to the immunoglobulin superfamily.

CD54 is a cell surface ligand of the lymphocyte integrin, LFA-1 and is known to play an important role in various cell-cell interactions in the immune system. Studies suggest that cross-linking of ICAM-1 using clone 1A29 induces calcium signalling (Etienne et al.).

Functionally Mouse anti Rat CD54 antibody, clone 1A29 inhibits homotypic aggregation of PHA blasts.

Product Details

Target Species
Rat
Product Form
Purified IgG conjugated to Alexa Fluor® 647 - liquid
Product Form
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Product Form
Purified IgG - liquid
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
Preservative Stabilisers
None present
Preservative Stabilisers
0.09%Sodium Azide
Carrier Free
Yes
Carrier Free
Yes
Immunogen
Rat Ax cells (a HEV derived cell line).
Approx. Protein Concentrations
IgG concentration 0.05 mg/ml
Approx. Protein Concentrations
Pack Size: 0.1 mg
IgG concentration 0.1 mg/ml
Pack Size: 0.5 mg
IgG concentration 0.5 mg/ml
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells from the PAI mouse myeloma cell line.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.

Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at -20oC only.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Pack Size: 0.1 mg
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Pack Size: 0.2 mg
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Shelf Life
18 months from date of despatch.
Shelf Life
18 months from date of despatch.
Shelf Life
18 months from date of despatch.

More Information

UniProt
Q00238 Related reagents
Entrez Gene
Icam1 Related reagents
GO Terms
GO:0001541 ovarian follicle development
GO:0001975 response to amphetamine
GO:0016021 integral to membrane
GO:0005178 integrin binding
GO:0005615 extracellular space
GO:0007569 cell aging
GO:0008360 regulation of cell shape
GO:0042493 response to drug
GO:0010212 response to ionizing radiation
GO:0010477 response to sulfur dioxide
GO:0014070 response to organic cyclic compound
GO:0031669 cellular response to nutrient levels
GO:0034698 response to gonadotropin stimulus
GO:0043200 response to amino acid stimulus
GO:0043498 cell surface binding
GO:0046688 response to copper ion
GO:0045429 positive regulation of nitric oxide biosynthetic process
GO:0045471 response to ethanol
GO:0045907 positive regulation of vasoconstriction
GO:0050900 leukocyte migration
GO:0051092 positive regulation of NF-kappaB transcription factor activity
GO:0051926 negative regulation of calcium ion transport
GO:0071222 cellular response to lipopolysaccharide
GO:0071312 cellular response to alkaloid
GO:0071333 cellular response to glucose stimulus
GO:0071347 cellular response to interleukin-1
GO:0071356 cellular response to tumor necrosis factor
GO:0071456 cellular response to hypoxia
Acknowledgements
This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
Regulatory
For research purposes only

Applications of CD54 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat
Flow Cytometry 1/25 Neat Pack Size: 0.1 mg
1/100 Pack Size: 0.5 mg
Flow Cytometry 1/25 1/50
Functional Assays
Immunofluorescence
Immunohistology - Frozen
Immunohistology - Paraffin 1
Immunoprecipitation
Western Blotting
Flow Cytometry 1/25 1/50
Immunofluorescence
Immunohistology - Frozen
Immunohistology - Paraffin 1
Immunoprecipitation
Western Blotting
  1. 1This clone is suitable for use on paraffin embedded material using target unmasking fluid HIS003B for this purpose.
  1. 1This clone is suitable for use on paraffin embedded material using target unmasking fluid HIS003B for this purpose.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Human anti Mouse IgG1:HRP HCA036P 0.1 mg E
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A 0.5 mg E WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F 0.5 mg F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P 0.5 mg E WB
Goat anti Mouse IgG (Fc):FITC STAR120F 1 mg C F
Goat anti Mouse IgG (Fc):HRP STAR120P 1 mg E WB
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A 1 ml F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B 1 mg C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 0.5 mg F
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 1 ml F
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A 1 mg C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P 1 mg E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA 0.1 mg F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B 1 mg F
Human anti Mouse IgG1:HRP HCA036P 0.1 mg E
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A 0.5 mg E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed) STAR117D649GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F 0.5 mg F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P 0.5 mg E WB
Goat anti Mouse IgG (Fc):FITC STAR120F 1 mg C F
Goat anti Mouse IgG (Fc):HRP STAR120P 1 mg E WB
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A 1 ml F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B 1 mg C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 0.5 mg F
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 1 ml F
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A 1 mg C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P 1 mg E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA 0.1 mg F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B 1 mg F

Negative Isotype Controls Available

Description Product Code Pack Size Applications List Price Quantity
Mouse IgG1 Negative Control:Alexa Fluor® 647 MCA1209A647 100 Tests/1ml F
Mouse IgG1 Negative Control:FITC MCA1209F 0.1 mg F
Mouse IgG1 Negative Control:Low Endotoxin MCA1209EL 0.5 mg F
Mouse IgG1 Negative Control MCA1209 0.1 mg F

Application Based External Images

Flow Cytometry

Functional Assays

Immunohistology - Frozen

Immunohistology - Paraffin

Immunoprecipitation

Western Blotting

Product Specific References

References for CD54 antibody

  1. Kawai, T. et al. (1999) Selective diapedesis of Th1 cells induced by endothelial cell RANTES.
    J Immunol. 163: 3269-78.
  2. Sato, N. et al. (2000) Roles of ICAM-1 for abnormal leukocyte recruitment in the microcirculation of bleomycin-induced fibrotic lung injury.
    Am J Respir Crit Care Med. 161: 1681-8.
  3. Etienne, S. et al. (1998) ICAM-1 signaling pathways associated with Rho activation in microvascular brain endothelial cells.
    J Immunol. 161 (10): 5755-61.
  4. Etienne-Manneville, S. et al. (2000) ICAM-1-coupled cytoskeletal rearrangements and transendothelial lymphocyte migration involve intracellular calcium signaling in brain endothelial cell lines.
    J Immunol. 165 (6): 3375-83.
  5. McKechnie, N. M. et al. (2002) Antigenic mimicry: Onchocerca volvulus antigen-specific T cells and ocular inflammation.
    Invest Ophthalmol Vis Sci. 43:411-8.
  6. Banerjee, S. et al. (2003) Development of organised conjunctival leucocyte aggregates after corneal transplantation in rats.
    Br J Ophthalmol. 2003 Dec;87(12):1515-22.
  7. Kielian, T. et al. (2000) Proinflammatory cytokine, chemokine, and cellular adhesion molecule expression during the acute phase of experimental brain abscess development.
    Am J Pathol. 157: 647-58.
  8. Trinh, L. et al. (2008) The corneal endothelium in an endotoxin-induced uveitis model: correlation between in vivo confocal microscopy and immunohistochemistry.
    Mol Vis. 14: 1149-56.
  9. Adamson, P. et al. (1999) Lymphocyte migration through brain endothelial cell monolayers involves signaling through endothelial ICAM-1 via a rho-dependent pathway.
    J Immunol. 162: 2964-73.
  10. Arsenović-Ranin, N. et al. (2000) A monoclonal antibody to the rat Crry/p65 antigen, a complement regulatory membrane protein, stimulates adhesion and proliferation of thymocytes.
    Immunology. 100: 334-44.
  11. Beck-Schimmer, B. et al. (2001) Hypoxia mediates increased neutrophil and macrophage adhesiveness to alveolar epithelial cells.
    Am J Respir Cell Mol Biol. 25: 780-7.
  12. Couty, J.P. (2007) PECAM-1 engagement counteracts ICAM-1-induced signaling in brain vascular endothelial cells.
    J Neurochem. 103: 793-801.
  13. Deng, H. et al. (2003) Mild hypothermia inhibits inflammation after experimental stroke and brain inflammation.
    Stroke. 34: 2495-501.
  14. Ikezumi, Y. et al. (2004) Macrophage-mediated renal injury is dependent on signaling via the JNK pathway.
    J Am Soc Nephrol. 15: 1775-84.
  15. Kanellis, J. et al. (2010) JNK signalling in human and experimental renal ischaemia/reperfusion injury.
    Nephrol Dial Transplant. 25: 2898-908.
  16. Westermann, D. et al. (2007) Cardioprotective and anti-inflammatory effects of interleukin converting enzyme inhibition in experimental diabetic cardiomyopathy.
    Diabetes. 56: 1834-41.
  17. Zhu, X. et al. (2003) Matrine protects sinusoidal endothelial cells from cold ischemia and reperfusion injury in rat orthotopic liver transplantation.
    Ann Clin Lab Sci. 33: 216-25.
  18. Choi, J.S. et al. (2011) Mild Hypothermia Attenuates Intercellular Adhesion Molecule-1 Induction via Activation of Extracellular Signal-Regulated Kinase-1/2 in a Focal Cerebral Ischemia Model.
    Stroke Res Treat. 2011: 846716.
  19. Azcutia V et al. (2010) Inflammation determines the pro-adhesive properties of high extracellular d-glucose in human endothelial cells in vitro and rat microvessels in vivo.
    PLoS One. 5 (4): e10091.
  20. Li, W. & Klein, S.L. (2012) Seoul virus-infected rat lung endothelial cells and alveolar macrophages differ in their ability to support virus replication and induce regulatory T cell phenotypes.
    J Virol. 86 (21): 11845-55.
  21. Gates, D. et al. (2012) Apo J/clusterin expression and secretion: evidence for 15-deoxy-Δ(12,14)-PGJ(2)-dependent mechanism.
    Biochim Biophys Acta. 1821 (2): 335-42.
  22. Li, Z. et al. (2015) Three-dimensional graphene foams loaded with bone marrow derived mesenchymal stem cells promote skin wound healing with reduced scarring.
    Mater Sci Eng C Mater Biol Appl. 57: 181-8.
  23. Gautier, S. et al. (2015) PPAR-Alpha Agonist Used at the Acute Phase of Experimental Ischemic Stroke Reduces Occurrence of Thrombolysis-Induced Hemorrhage in Rats.
    PPAR Research. 2015: 1-6.
  24. Liu, Y.C. et al. (2013) A biodegradable, sustained-released, prednisolone acetate microfilm drug delivery system effectively prolongs corneal allograft survival in the rat keratoplasty model.
    PLoS One. 8 (8): e70419.
  25. Ichihara, Y. et al. (2018) Self-assembling peptide hydrogel enables instant epicardial coating of the heart with mesenchymal stromal cells for the treatment of heart failure.
    Biomaterials. 154: 12-23.