CD31 antibody | TLD-3A12

Mouse anti Rat CD31

Product Type
Monoclonal Antibody
Clone
TLD-3A12
Isotype
IgG1
Specificity
CD31

Product Code Applications Pack Size List Price Your Price Qty
MCA1334G
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C E F IF WB 0.25 mg loader
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loader
MCA1334GA
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C E F IF WB 0.1 mg loader
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loader
Search for Batch Specific Datasheets

Mouse anti Rat CD31 antibody, clone TLD-3A12 recognizes rat PECAM-1 (CD31), a 661 amino acid type 1 transmembrane protein expressed primarily on endothelial cells, platelets and leucocytes.

Clone TLD-3A12 has been shown to partially block the proliferative response of antigen-specific CD4+ T cells to antigen-presenting cells and relevant antigen (Stevenson, K.S. et al.2009).

Mouse anti Rat CD31 antibody, clone TLD-3A12 is suitable for use in IHC on formalin-fixed paraffin-embedded sections pre-treated with 0.2M boric acid, pH7.0. (Wilson et al. 2007). Mouse anti Rat CD31, clone TLD-3A12 has been shown to be cross-reactive with endothelial cells derived from rhesus macaque (Maclean et al. 2001)

Target Species
Rat
Species Cross-Reactivity
Target SpeciesCross Reactivity
Rhesus Monkey
Pig
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% sodium azide (NaN3)
Carrier Free
Yes
Immunogen
Activated, Lewis rat derived microglial cells.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised BALB/c mouse were fused with cells of the mouse SP2 myeloma cell line.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Flow Cytometry 1/10 1/100
Immunofluorescence
Immunohistology - Frozen 1/10 1/100
Western Blotting
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10μl of the suggested working dilution to label 106 cells in 100μl

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References for CD31 antibody

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Further Reading

  1. Male, D. et al. (1995) An interferon-inducible molecule on brain endothelium which controls lymphocyte adhesion mediated by integrins.
    Immunology. 84 (3): 453-60.

Immunofluorescence

Immunohistology - Frozen

Immunohistology - Paraffin

Western Blotting

Synonyms
PECAM-1
RRID
AB_322925
UniProt
Q3SWT0
Entrez Gene
Pecam1
GO Terms
GO:0005515 protein binding
GO:0002687 positive regulation of leukocyte migration
GO:0007155 cell adhesion
GO:0016021 integral to membrane
GO:0006928 cellular component movement
GO:0009986 cell surface
GO:0030054 cell junction
GO:0034097 response to cytokine stimulus
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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