CD31 antibody | TLD-3A12
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Mouse anti Rat CD31
- Product Type
- Monoclonal Antibody
|Mouse anti Rat CD31 antibody, clone TLD-3A12 recognizes rat PECAM-1 (CD31), a 661 amino acid type 1 transmembrane protein expressed primarily on endothelial cells, platelets and leucocytes.
Clone TLD-3A12 has been shown to partially block the proliferative response of antigen-specific CD4+ T cells to antigen-presenting cells and relevant antigen (Stevenson, K.S. et al.2009).
Mouse anti Rat CD31 antibody, clone TLD-3A12 is suitable for use in IHC on formalin-fixed paraffin-embedded sections pre-treated with 0.2M boric acid, pH7.0. (Wilson et al. 2007). Mouse anti Rat CD31, clone TLD-3A12 has been shown to be cross-reactive with endothelial cells derived from rhesus macaque (Maclean et al. 2001)
- Target Species
- Species Cross-Reactivity
Target Species Cross Reactivity Rhesus Monkey Pig
- N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% sodium azide (NaN3)
- Carrier Free
- Activated, Lewis rat derived microglial cells.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised BALB/c mouse were fused with cells of the mouse SP2 myeloma cell line.
- For research purposes only
- 12 months from date of despatch
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
|Application Name||Verified||Min Dilution||Max Dilution|
|Immunohistology - Frozen||1/10||1/100|
- Flow Cytometry
- Use 10μl of the suggested working dilution to label 106 cells in 100μl
|Description||Product Code||Applications||Pack Size||List Price||Your Price||Quantity|
|Mouse IgG1 Negative Control||MCA1209||F||0.1 mg||Log in|
|List Price||Your Price|
|Description||Mouse IgG1 Negative Control|
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References for CD31 antibody
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Graham, M.J. et al. (1998) In vivo distribution and metabolism of a phosphorothioate oligonucleotide within rat liver after intravenous administration.
J Pharmacol Exp Ther. 286: 447-58.
MacLean, A.G. et al. (2001) Rhesus macaque brain microvessel endothelial cells behave in a manner phenotypically distinct from umbilical vein endothelial cells.
J Neuroimmunol. 118: 223-32.
Frye, C.A. & Patrick, C.W. Jr (2002) Isolation and culture of rat microvascular endothelial cells.
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Seegers, H.C. et al. (2003) Enhancement of angiogenesis by endogenous substance P release and neurokinin-1 receptors during neurogenic inflammation.
J Pharmacol Exp Ther. 306: 8-12.
Haywood, L. et al. (2003) Inflammation and angiogenesis in osteoarthritis.
Arthritis Rheum. 48: 2173-7.
Wilson, E. et al. (2007) An evaluation of the immunohistochemistry benefits of boric acid antigen retrieval on rat decalcified joint tissues.
J Immunol Methods. 322: 137-42.
View The Latest Product References
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- Entrez Gene
- GO Terms
- GO:0005515 protein binding
- GO:0002687 positive regulation of leukocyte migration
- GO:0007155 cell adhesion
- GO:0016021 integral to membrane
- GO:0006928 cellular component movement
- GO:0009986 cell surface
- GO:0030054 cell junction
- GO:0034097 response to cytokine stimulus
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