CD2 antibody | OX-34

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Mouse anti Rat CD2:Alexa Fluor® 488

Mouse anti Rat CD2:Alexa Fluor® 647

Mouse anti Rat CD2

Product Type
Monoclonal Antibody
Clone
OX-34
Isotype
IgG2a
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA154A488 F 100 Tests/1ml
MCA154A647 F 100 Tests/1ml
MCA154GA C F IF IP P* 0.1 mg
MCA154R C F IF IP P* 0.25 mg
Mouse anti Rat CD2 antibody, clone OX-34 recognizes a determinant on thymocytes and peripheral T-cells but it does not bind to B cells or peritoneal macrophages. The antigen recognized by this antibody is a 50-54 kDa glycoprotein, homolog of the human CD2 antigen (Williams et al. 1987).

Product Details

Target Species
Rat
Product Form
Purified IgG conjugated to Alexa Fluor® 488 - liquid
Product Form
Purified IgG conjugated to Alexa Fluor® 647 - liquid
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein G
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
Preservative Stabilisers
0.09%Sodium Azide
Carrier Free
Yes
Immunogen
Activated rat T helper cells.
Approx. Protein Concentrations
IgG concentration 0.05 mg/ml
Approx. Protein Concentrations
IgG concentration 0.05 mg/ml
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the NS1 mouse myeloma cell line.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.

Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.

Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Shelf Life
18 months from date of despatch.
Shelf Life
18 months from date of despatch.

More Information

UniProt
P08921 Related reagents
Entrez Gene
Cd2 Related reagents
GO Terms
GO:0001948 glycoprotein binding
GO:0003823 antigen binding
GO:0016021 integral to membrane
GO:0005615 extracellular space
GO:0030971 receptor tyrosine kinase binding
GO:0009986 cell surface
GO:0016337 cell-cell adhesion
GO:0019901 protein kinase binding
GO:0042110 T cell activation
GO:0042803 protein homodimerization activity
GO:0043234 protein complex
GO:0043621 protein self-association
GO:0044214 fully spanning the plasma membrane
GO:0045121 membrane raft
Acknowledgements
This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
Acknowledgements
This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
Regulatory
For research purposes only

Applications of CD2 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat 1/10
Flow Cytometry Neat
Flow Cytometry 1/50
Immunofluorescence
Immunohistology - Frozen
Immunohistology - Paraffin 1
Immunoprecipitation
  1. 1We recommend the use of PLP fixation for optimal results, see Whiteland et al.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.* This clone has been described as reacting with paraffin-embedded material following PLP fixation.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Human anti Mouse IgG2a:FITC HCA037F 0.1 mg F
Human anti Mouse IgG2a:HRP HCA037P 0.1 mg E
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A 0.5 mg E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed) STAR117D549GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed) STAR117D649GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA 0.1 mg F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F 0.5 mg F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P 0.5 mg E WB
Goat anti Mouse IgG (Fc):FITC STAR120F 1 mg C F
Goat anti Mouse IgG (Fc):HRP STAR120P 1 mg E WB
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A 1 ml F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B 1 mg C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 0.5 mg F
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 1 ml F
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 0.5 mg C E P
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A 1 mg C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P 1 mg E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA 0.1 mg F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B 1 mg F

Negative Isotype Controls Available

Description Product Code Pack Size Applications List Price Quantity
Mouse IgG2a Negative Control:Alexa Fluor® 488 MCA1210A488 100 Tests/1ml F
Mouse IgG2a Negative Control:Alexa Fluor® 647 MCA1210A647 100 Tests/1ml F
Mouse IgG2a Negative Control MCA1210 100 Tests F

Application Based External Images

Immunofluorescence

Immunohistology - Frozen

Immunoprecipitation

Western Blotting

Product Specific References

References for CD2 antibody

  1. Williams, A.F. et al. (1987) Similarities in sequences and cellular expression between rat CD2 and CD4 antigens.
    J Exp Med. 165 (2): 368-80.
  2. Barclay, A.N. (1981) The localization of populations of lymphocytes defined by monoclonal antibodies in rat lymphoid tissues.
    Immunology. 42 (4): 593-600.
  3. Whiteland, J.L. et al. (1995) Immunohistochemical detection of T-cell subsets and other leukocytes in paraffin-embedded rat and mouse tissues with monoclonal antibodies.
    J Histochem Cytochem. 43 (3): 313-20.
  4. Baker, S.C. et al. (2011) Cellular Integration and Vascularisation Promoted by a Resorbable, Particulate-Leached, Cross-Linked Poly(ε-caprolactone) Scaffold.
    Macromol Biosci. 11: 618-27.
  5. Romani, P. et al. (2009) Cell survival and polarity of Drosophila follicle cells require the activity of ecdysone receptor B1 isoform.
    Genetics. 181: 165-75.
  6. Stybayeva, G. et al. (2010) Lensfree holographic imaging of antibody microarrays for high-throughput detection of leukocyte numbers and function.
    Anal Chem. 82: 3736-44.
  7. Bastock, R. et al. (2003) Strabismus is asymmetrically localised and binds to Prickle and Dishevelled during Drosophila planar polarity patterning.
    Development. 130: 3007-14.
  8. Brückner, K. et al. (2000) Glycosyltransferase activity of Fringe modulates Notch-Delta interactions.
    Nature. 406: 411-5.
  9. Liversidge, J. et al. (2002) Nitric oxide mediates apoptosis through formation of peroxynitrite and Fas/Fas-ligand interactions in experimental autoimmune uveitis.
    Am J Pathol. 160: 905-16.
  10. Sarpal, R. et al. (2012) Mutational analysis supports a core role for Drosophila α-catenin in adherens junction function.
    J Cell Sci. 125: 233-45.
  11. Zhang, H. et al. (2011) Basic residues in the T-cell receptor ζ cytoplasmic domain mediate membrane association and modulate signaling.
    Proc Natl Acad Sci U S A. 108: 19323-8.
  12. Heck, B.W. et al. (2012) The transcriptional corepressor SMRTER influences both Notch and ecdysone signaling during Drosophila development.
    Biol Open. 1 (3): 182-96.
  13. Clark, I.B. et al. (2011) Fibroblast growth factor signalling controls successive cell behaviours during mesoderm layer formation in Drosophila.
    Development. 138: 2705-15.
  14. Domanitskaya, E. and Schüpbach, T. (2012) CoREST acts as a positive regulator of Notch signaling in the follicle cells of Drosophila melanogaster.
    J Cell Sci. 125: 399-410.
  15. Dragovic, R.A. et al. (2015) Isolation of syncytiotrophoblast microvesicles and exosomes and their characterisation by multicolour flow cytometry and fluorescence Nanoparticle Tracking Analysis.
    Methods. 87: 64-74.