CD2 antibody | OX-34
Mouse anti Rat CD2 antibody, clone OX-34 used for the detection of rat CD2 fusion proteins in Drosophila by immunofluorescence.
Image caption:
Ey and Toy are redundantly required to activate stg-FMW.(D—G) Analysis of the expression of stg-FMW upon mutation of BS1 or BS2. Representative L3 eye imaginal discs carrying the wild type stg-FMW (D), and the mutated versions stg*BS1 (E) and stg*BS2 (F) of the enhancer. (G) Expression of GFP is not affected upon mutation of BS1 or BS2 in stg-FMW. Simultaneous mutation of BS1 and BS2 (stg*BS1+*BS2) abolishes GFP expression in the eye field; small GFP dots can be detected but only at the dorsal and ventral margins of the eye disc. Discs are outlined by the white dashed lines. The position of the MF is indicated by the white arrow and yellow dashed line. (H—J) Clones of ey (H) or toy (I) RNAi, in the eye imaginal disc. Clones are outlined and marked by the absence of CD2 (red). Expression of stg-FMW (green) is not affected by downregulation of Ey or Toy. (J) Eye disc containing ey and toy double mutant clones, stained for CD2 (red) and stg-FMW (green). In clones of cells double mutant for ey and toy the expression of stg-FMW is lost. White arrow indicates the position of the MF. In all images anterior is to the left.
From: Lopes CS, Casares F (2015)
Eye Selector Logic for a Coordinated Cell Cycle Exit.
PLoS Genet 11(2): e1004981.
Mouse anti Rat CD2 antibody, clone OX-34 used for the detection of rat CD2 fusion proteins in Drosophila by immunofluorescence.
Image caption:
Hth defines the anterior domain of stg-FMW expression. (A) Clone of cells ectopically expressing Hth-HA, labeled in magenta, and stained for stg-FMW (green). (A’) Expression of stg-FMW is only repressed within the anterior domain of the clone. (B) Eye disc containing clones of a null allele of hth (hthP2), stained for CD2 (red) and stg-FMW (green). Clone cells are labeled by the absence of CD2. Within the clone stg-FMW expression occurs in a few cell rows anterior to its normal domain of expression. Arrow indicates premature expression of GFP. The vertical dashed line limits the most anterior expression of stg-FMW in wild-type tissue. The horizontal line represents the width of stg-FWW expression in wild-type tissue. (C) Mutation of the Hth BS (stg*Hth) does not affect the spatial or temporal expression of stg-FMW.
From: Lopes CS, Casares F (2015)
Eye Selector Logic for a Coordinated Cell Cycle Exit.
PLoS Genet 11(2): e1004981.
Mouse anti Rat CD2 antibody, clone OX-34 used for the detection of rat CD2 fusion proteins in Drosophila by immunofluorescence.
Image caption:
So/Eya are positive regulators of stg-FMW. (A) Eye imaginal disc with clones of cells mutant for eya, labeled by the absence of CD2 (red). (A′) Within the clone cells, stg-FMW expression (green) is not activated. (A′′) Hth expression (blue) is maintained in eya RNAi cells. (B) MARCM eya-hth- clones (GFP) stained for stg-FMW (lacZ- red) (B′) and Hth (B&prime, &prime)(blue), showing that eya is required for stg-FMW expression. Clones are outlined. The asterisk (*) in A indicates the normal expression pattern of Hth, during L3, in pigment progenitor cells posterior to the MF [17]. In all images anterior is to the left.
From: Lopes CS, Casares F (2015) Eye Selector Logic for a Coordinated Cell Cycle Exit. PLoS Genet 11(2): e1004981.
Mouse anti Rat CD2 antibody, clone OX-34 used for the detection of rat CD2 fusion proteins in Drosophila by immunofluorescence.
Image caption:
Hth defines the anterior domain of stg-FMW expression. (A) Clone of cells ectopically expressing Hth-HA, labeled in magenta, and stained for stg-FMW (green). (A′) Expression of stg-FMW is only repressed within the anterior domain of the clone. (B) Eye disc containing clones of a null allele of hth (hthP2), stained for CD2 (red) and stg-FMW (green). Clone cells are labeled by the absence of CD2. Within the clone stg-FMW expression occurs in a few cell rows anterior to its normal domain of expression. Arrow indicates premature expression of GFP. The vertical dashed line limits the most anterior expression of stg-FMW in wild-type tissue. The horizontal line represents the width of stg-FWW expression in wild-type tissue. (C) Mutation of the Hth BS (stg*Hth) does not affect the spatial or temporal expression of stg-FMW.
From: Lopes CS, Casares F (2015)
Eye Selector Logic for a Coordinated Cell Cycle Exit.
PLoS Genet 11(2): e1004981.
Mouse anti Rat CD2 antibody, clone OX-34 used for the detection of rat CD2 fusion proteins in Drosophila by immunofluorescence.
Image caption:
Expression of stg mRNA is not affected in ey2 mutants. (A—E) In situ hybridization of stg mRNA on ey2 homozygous discs shows that the levels and expression pattern of stg are not significantly affected, despite the growth defect and abnormal MF progression. The solid white line depicts the MF in ey2 and the white dashed line represents the position where the MF should be if progression was uniform in dorsal and ventral domains. (B, C) Higher magnification images of the discs in (A). Representative ey2 eye imaginal discs are shown. (F-G) The activity of stg-FMW enhancer is not affected upon ey mutation. Eye-antennal imaginal discs mutant for ey2 showing expression of GFP driven by stg-FMW. GFP expression (green) is not affected despite the evident irregular progression of the MF. Eya is shown in red. (H-K) One Pax6 BS suffices for enhancer activity. Eye imaginal discs containing clones of ey RNAi (H, I) and toy RNAi (J, K) in the presence of either stg-BS1* or stg-BS2* mutant stg-FMW enhancer. Clones are marked by the absence of CD2 (red) and outlined. GFP expression driven by either stg-BS1* or stg-BS2* is shown in green. In none of these combinations, GFP expression is affected, indicating that Ey and Toy do not show functional preference for either BS1 or BS2.
From: Lopes CS, Casares F (2015)
Eye Selector Logic for a Coordinated Cell Cycle Exit.
PLoS Genet 11(2): e1004981.
Mouse anti Rat CD2 antibody, clone OX-34 used for the detection of rat CD2 fusion proteins in Drosophila by immunofluorescence.
Image caption:
Activation of the Dpp or the Hh pathway does not lead to precocious activation of stg-FMW. Clones of gain of function of an activated form of thickveins (tkvQD) (A-C) and Cubitus interruptus (ci) (D) showing that the Dpp and Hh signalling pathways do not suffice for activation of stg-FMW in other domains of the eye imaginal disc. Clones are labelled by the absence of CD2 (magenta). The activity of stg-FMW is detected by the expression of GFP (green). Clones are outlined. Anterior is to the left.
From: Lopes CS, Casares F (2015)
Eye Selector Logic for a Coordinated Cell Cycle Exit. PLoS Genet 11(2): e1004981.
Mouse anti Rat CD2 antibody, clone OX-34 used for the detection of rat CD2 tagged fusion protein in Drosophila by immunofluorescence.
Image caption:
Protrusion formation during mesoderm flattening. (A,B) Two-photon time-lapse sequence of mesoderm cells expressing GFP-Actin in the period following EMT (ventral imaging protocol; see Fig. S1 in the supplementary material). VM, ventral midline; DL, dorsolateral ectoderm. (A) Intensity-rendered 3D reconstructions (see Movie 4 in the supplementary material); (B) transverse sections (see Movie 5 in the supplementary material). Cells at the dorsal edge (DECs) protrude radially (white arrows) and move towards the ectoderm (yellow arrows indicate ventral cells protruding into the ectoderm). (C) DEC (white arrows) and more ventral cell (yellow arrows) protrusions visualised in a wild-type twi::CD2-expressing embryo fixed during mesoderm flattening (3D reconstructed image shown rotating in Movie 6 in the supplementary material). Embryo in C is shown in ventrolateral view, anterior towards the left.
From: Clark IB, Muha V, Klingseisen A, Leptin M, Müller HA.
Fibroblast growth factor signalling controls successive cell behaviours during mesoderm layer formation in Drosophila.
Development. 2011 Jul;138(13):2705-15.
Mouse anti Rat CD2 antibody, clone OX-34 used for the detection of rat CD2 tagged fusion protein in Drosophila by immunofluorescence.
Image caption:
A role for Cdc42 at the mesoderm/ectoderm interface. (A) Comparison of dorsal and radial protrusions (from twi::CD2 stainings) in wild-type embryos (yellow bars) and embryos expressing UAS::Cdc[N17] driven by twi::Gal4. The asterisks indicate statistically significant differences in the number of radial protrusions [P<0.05 (n=5); two-sample t-test]. There is no difference in the number of dorsal protrusions [P>0.05 (n=5); two-sample t-test]. Data are mean±s.d. (B) Wild-type and Cdc42 mutant embryos (obtained from Cdc424/Cdc426 trans-heterozygous females) were stained for Twi (stages 8-10) and for Eve (stage 12). Note irregular distribution of mesoderm nuclei in Cdc42 mutants compared with wild type, indicating defects in mesoderm spreading. Arrowheads in stage 12 embryos indicate position of segmental Eve-positive dorsal mesoderm cells; note the strong reduction in the number of Eve-positive hemi-segments. (C) E-cadherin immunostaining of embryos (stage 9) expressing UAS::Cdc[N17] driven by twi::Gal4 compared with wild type. E-cadherin accumulates at the ectoderm-mesoderm interface (mesoderm is marked in red with CD2), which is absent in embryos expressing dominant-negative Cdc42.
From: Clark IB, Muha V, Klingseisen A, Leptin M, Müller HA. Fibroblast growth factor signalling controls successive cell behaviours during mesoderm layer formation in Drosophila.
Development. 2011 Jul;138(13):2705-15.
Mouse anti Rat CD2:Alexa Fluor® 488
Mouse anti Rat CD2:Alexa Fluor® 647
Mouse anti Rat CD2
- Product Type
- Monoclonal Antibody
- Clone
- OX-34
- Isotype
- IgG2a
| Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|
| MCA154A488 | F | 100 Tests/1ml |
|
|
| MCA154A647 | F | 100 Tests/1ml |
|
|
| MCA154GA | C F IF IP P* | 0.1 mg |
|
|
| MCA154R | C F IF IP P* | 0.25 mg |
|
Mouse anti Rat CD2 antibody, clone OX-34 recognizes a determinant on thymocytes and peripheral T-cells but it does not bind to B cells or peritoneal macrophages. The antigen recognized by this antibody is a 50-54 kDa glycoprotein, homolog of the human CD2 antigen (Williams et al. 1987).
Product Details
- Target Species
- Rat
- Product Form
- Purified IgG conjugated to Alexa Fluor® 488 - liquid
- Product Form
- Purified IgG conjugated to Alexa Fluor® 647 - liquid
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin - Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin - Preservative Stabilisers
0.09% Sodium Azide - Carrier Free
- Yes
- Immunogen
- Activated rat T helper cells.
- Approx. Protein Concentrations
- IgG concentration 0.05 mg/ml
- Approx. Protein Concentrations
- IgG concentration 0.05 mg/ml
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised BALB/c mice were fused with cells of the NS1 mouse myeloma cell line.
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 18 months from date of despatch.
- Guarantee
- 18 months from date of despatch.
- Guarantee
- 18 months from date of despatch.
More Information
- UniProt
- P08921 Related reagents
- Entrez Gene
- Cd2 Related reagents
- GO Terms
- GO:0001948 glycoprotein binding
- GO:0003823 antigen binding
- GO:0016021 integral to membrane
- GO:0005615 extracellular space
- GO:0030971 receptor tyrosine kinase binding
- GO:0009986 cell surface
- GO:0016337 cell-cell adhesion
- GO:0019901 protein kinase binding
- GO:0042110 T cell activation
- GO:0042803 protein homodimerization activity
- GO:0043234 protein complex
- GO:0043621 protein self-association
- GO:0044214 fully spanning the plasma membrane
- GO:0045121 membrane raft
- Acknowledgements
- This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
- Acknowledgements
- This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
- Regulatory
- For research purposes only
Applications of CD2 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry | Neat | 1/10 | |
| Flow Cytometry | Neat | ||
| Flow Cytometry | 1/50 | ||
| Immunofluorescence | |||
| Immunohistology - Frozen | |||
| Immunohistology - Paraffin 1 | |||
| Immunoprecipitation |
- 1We recommend the use of PLP fixation for optimal results, see Whiteland et al.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.* This clone has been described as reacting with paraffin-embedded material following PLP fixation.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Copyright © 2020 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
Negative Isotype Controls Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Mouse IgG2a Negative Control:Alexa Fluor® 488 | MCA1210A488 | F | 100 Tests/1ml |
|
|
| Mouse IgG2a Negative Control:Alexa Fluor® 647 | MCA1210A647 | F | 100 Tests/1ml |
|
|
| Mouse IgG2a Negative Control | MCA1210 | F | 100 Tests |
|
Application Based External Images
Immunofluorescence
Immunohistology - Frozen
Immunoprecipitation
Western Blotting
Product Specific References
References for CD2 antibody
-
Williams, A.F. et al. (1987) Similarities in sequences and cellular expression between rat CD2 and CD4 antigens.
J Exp Med. 165 (2): 368-80. -
Barclay, A.N. (1981) The localization of populations of lymphocytes defined by monoclonal antibodies in rat lymphoid tissues.
Immunology. 42 (4): 593-600. -
Whiteland, J.L. et al. (1995) Immunohistochemical detection of T-cell subsets and other leukocytes in paraffin-embedded rat and mouse tissues with monoclonal antibodies.
J Histochem Cytochem. 43 (3): 313-20. -
Baker, S.C. et al. (2011) Cellular Integration and Vascularisation Promoted by a Resorbable, Particulate-Leached, Cross-Linked Poly(ε-caprolactone) Scaffold.
Macromol Biosci. 11: 618-27. -
Romani, P. et al. (2009) Cell survival and polarity of Drosophila follicle cells require the activity of ecdysone receptor B1 isoform.
Genetics. 181: 165-75. -
Stybayeva, G. et al. (2010) Lensfree holographic imaging of antibody microarrays for high-throughput detection of leukocyte numbers and function.
Anal Chem. 82: 3736-44. -
Bastock, R. et al. (2003) Strabismus is asymmetrically localised and binds to Prickle and Dishevelled during Drosophila planar polarity patterning.
Development. 130: 3007-14. -
Brückner, K. et al. (2000) Glycosyltransferase activity of Fringe modulates Notch-Delta interactions.
Nature. 406: 411-5. -
Liversidge, J. et al. (2002) Nitric oxide mediates apoptosis through formation of peroxynitrite and Fas/Fas-ligand interactions in experimental autoimmune uveitis.
Am J Pathol. 160: 905-16. -
Sarpal, R. et al. (2012) Mutational analysis supports a core role for Drosophila α-catenin in adherens junction function.
J Cell Sci. 125: 233-45. -
Zhang, H. et al. (2011) Basic residues in the T-cell receptor ζ cytoplasmic domain mediate membrane association and modulate signaling.
Proc Natl Acad Sci U S A. 108: 19323-8. -
Heck, B.W. et al. (2012) The transcriptional corepressor SMRTER influences both Notch and ecdysone signaling during Drosophila development.
Biol Open. 1 (3): 182-96. -
Clark, I.B. et al. (2011) Fibroblast growth factor signalling controls successive cell behaviours during mesoderm layer formation in Drosophila.
Development. 138: 2705-15. -
Domanitskaya, E. and Schüpbach, T. (2012) CoREST acts as a positive regulator of Notch signaling in the follicle cells of Drosophila melanogaster.
J Cell Sci. 125: 399-410. -
Dragovic, R.A. et al. (2015) Isolation of syncytiotrophoblast microvesicles and exosomes and their characterisation by multicolour flow cytometry and fluorescence Nanoparticle Tracking Analysis.
Methods. 87: 64-74.
