CD2 antibody | OX-34

Product Code Applications Pack Size List Price Your Price Qty
MCA154GA
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SDS Safety Datasheet SDS
C F IF IP P * 0.1 mg loader
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loader
MCA154R
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SDS Safety Datasheet SDS
C F IF IP P * 0.25 mg loader
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loader
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Mouse anti Rat CD2 antibody, clone OX-34 recognizes a determinant on thymocytes and peripheral T-cells but it does not bind to B cells or peritoneal macrophages. The antigen recognized by this antibody is a 50-54 kDa glycoprotein, homolog of the human CD2 antigen (Williams et al. 1987).

Target Species
Rat
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Carrier Free
Yes
Immunogen
Activated rat T helper cells.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the NS1 mouse myeloma cell line.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/50
Immunofluorescence
Immunohistology - Frozen
Immunohistology - Paraffin 1
Immunoprecipitation
  1. 1We recommend the use of PLP fixation for optimal results, see Whiteland et al.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.* This clone has been described as reacting with paraffin-embedded material following PLP fixation.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.

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Description Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (Fc):FITC
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Description Goat anti Mouse IgG (Fc):HRP
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Description Rabbit F(ab')2 anti Mouse IgG:RPE
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Description Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)
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Description Goat anti Mouse IgG:FITC (Rat Adsorbed)
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Description Goat anti Mouse IgG:RPE (Rat Adsorbed)
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Description Goat anti Mouse IgG:HRP (Rat Adsorbed)
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Description Goat anti Mouse IgG/A/M:Alk. Phos.
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Description Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)
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Description Rabbit F(ab')2 anti Mouse IgG:Dylight®800
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Description Rabbit F(ab')2 anti Mouse IgG:FITC

Description Product Code Applications Pack Size List Price Your Price Quantity
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Description Mouse IgG2a Negative Control

References for CD2 antibody

  1. Williams, A.F. et al. (1987) Similarities in sequences and cellular expression between rat CD2 and CD4 antigens.
    J Exp Med. 165 (2): 368-80.
  2. Barclay, A.N. (1981) The localization of populations of lymphocytes defined by monoclonal antibodies in rat lymphoid tissues.
    Immunology. 42 (4): 593-600.
  3. Whiteland, J.L. et al. (1995) Immunohistochemical detection of T-cell subsets and other leukocytes in paraffin-embedded rat and mouse tissues with monoclonal antibodies.
    J Histochem Cytochem. 43 (3): 313-20.
  4. Baker, S.C. et al. (2011) Cellular Integration and Vascularisation Promoted by a Resorbable, Particulate-Leached, Cross-Linked Poly(ε-caprolactone) Scaffold.
    Macromol Biosci. 11: 618-27.
  5. Romani, P. et al. (2009) Cell survival and polarity of Drosophila follicle cells require the activity of ecdysone receptor B1 isoform.
    Genetics. 181: 165-75.
  6. Stybayeva, G. et al. (2010) Lensfree holographic imaging of antibody microarrays for high-throughput detection of leukocyte numbers and function.
    Anal Chem. 82: 3736-44.
  7. Bastock, R. et al. (2003) Strabismus is asymmetrically localised and binds to Prickle and Dishevelled during Drosophila planar polarity patterning.
    Development. 130: 3007-14.
  8. Brückner, K. et al. (2000) Glycosyltransferase activity of Fringe modulates Notch-Delta interactions.
    Nature. 406: 411-5.
  9. View The Latest Product References
  10. Liversidge, J. et al. (2002) Nitric oxide mediates apoptosis through formation of peroxynitrite and Fas/Fas-ligand interactions in experimental autoimmune uveitis.
    Am J Pathol. 160: 905-16.
  11. Sarpal, R. et al. (2012) Mutational analysis supports a core role for Drosophila α-catenin in adherens junction function.
    J Cell Sci. 125: 233-45.
  12. Zhang, H. et al. (2011) Basic residues in the T-cell receptor ζ cytoplasmic domain mediate membrane association and modulate signaling.
    Proc Natl Acad Sci U S A. 108: 19323-8.
  13. Heck, B.W. et al. (2012) The transcriptional corepressor SMRTER influences both Notch and ecdysone signaling during Drosophila development.
    Biol Open. 1 (3): 182-96.
  14. Clark, I.B. et al. (2011) Fibroblast growth factor signalling controls successive cell behaviours during mesoderm layer formation in Drosophila.
    Development. 138: 2705-15.
  15. Domanitskaya, E. and Schüpbach, T. (2012) CoREST acts as a positive regulator of Notch signaling in the follicle cells of Drosophila melanogaster.
    J Cell Sci. 125: 399-410.
  16. Dragovic, R.A. et al. (2015) Isolation of syncytiotrophoblast microvesicles and exosomes and their characterisation by multicolour flow cytometry and fluorescence Nanoparticle Tracking Analysis.
    Methods. 87: 64-74.
  17. Zecca, M. & Struhl, G. (2021) A unified mechanism for the control of Drosophila. wing growth by the morphogens Decapentaplegic and Wingless.
    PLoS Biol. 19 (3): e3001111.

Immunofluorescence

Immunohistology - Frozen

Immunoprecipitation

Synonyms
E-Rosette Receptor
LFA-2
RRID
AB_321239
UniProt
P08921
Entrez Gene
Cd2
GO Terms
GO:0001948 glycoprotein binding
GO:0003823 antigen binding
GO:0016021 integral to membrane
GO:0005615 extracellular space
GO:0030971 receptor tyrosine kinase binding
GO:0009986 cell surface
GO:0016337 cell-cell adhesion
GO:0019901 protein kinase binding
GO:0042110 T cell activation
GO:0042803 protein homodimerization activity
GO:0043234 protein complex
GO:0043621 protein self-association
GO:0044214 fully spanning the plasma membrane
GO:0045121 membrane raft
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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