CD2 antibody | OX-34











Mouse anti Rat CD2:Alexa Fluor® 488
Mouse anti Rat CD2:Alexa Fluor® 647
Mouse anti Rat CD2
- Product Type
- Monoclonal Antibody
- Clone
- OX-34
- Isotype
- IgG2a
Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|
MCA154A488 | F | 100 Tests/1ml |
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MCA154A647 | F | 100 Tests/1ml |
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MCA154GA | C F IF IP P* | 0.1 mg |
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MCA154R | C F IF IP P* | 0.25 mg |
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Product Details
- Target Species
- Rat
- Product Form
- Purified IgG conjugated to Alexa Fluor® 488 - liquid
- Product Form
- Purified IgG conjugated to Alexa Fluor® 647 - liquid
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin - Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin - Preservative Stabilisers
0.09% Sodium Azide - Carrier Free
- Yes
- Immunogen
- Activated rat T helper cells.
- Approx. Protein Concentrations
- IgG concentration 0.05 mg/ml
- Approx. Protein Concentrations
- IgG concentration 0.05 mg/ml
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised BALB/c mice were fused with cells of the NS1 mouse myeloma cell line.
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 18 months from date of despatch.
- Guarantee
- 18 months from date of despatch.
- Guarantee
- 18 months from date of despatch.
More Information
- UniProt
- P08921 Related reagents
- Entrez Gene
- Cd2 Related reagents
- GO Terms
- GO:0001948 glycoprotein binding
- GO:0003823 antigen binding
- GO:0016021 integral to membrane
- GO:0005615 extracellular space
- GO:0030971 receptor tyrosine kinase binding
- GO:0009986 cell surface
- GO:0016337 cell-cell adhesion
- GO:0019901 protein kinase binding
- GO:0042110 T cell activation
- GO:0042803 protein homodimerization activity
- GO:0043234 protein complex
- GO:0043621 protein self-association
- GO:0044214 fully spanning the plasma membrane
- GO:0045121 membrane raft
- Acknowledgements
- This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
- Acknowledgements
- This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
- Regulatory
- For research purposes only
Applications of CD2 antibody
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
Flow Cytometry | Neat | 1/10 | |
Flow Cytometry | Neat | ||
Flow Cytometry | 1/50 | ||
Immunofluorescence | |||
Immunohistology - Frozen | |||
Immunohistology - Paraffin 1 | |||
Immunoprecipitation |
- 1We recommend the use of PLP fixation for optimal results, see Whiteland et al.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Secondary Antibodies Available
Negative Isotype Controls Available
Description | Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|---|
Mouse IgG2a Negative Control:Alexa Fluor® 488 | MCA1210A488 | F | 100 Tests/1ml |
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Mouse IgG2a Negative Control:Alexa Fluor® 647 | MCA1210A647 | F | 100 Tests/1ml |
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Mouse IgG2a Negative Control | MCA1210 | F | 100 Tests |
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Application Based External Images
Immunofluorescence
Immunohistology - Frozen
Immunoprecipitation
Western Blotting
Product Specific References
References for CD2 antibody
-
Williams, A.F. et al. (1987) Similarities in sequences and cellular expression between rat CD2 and CD4 antigens.
J Exp Med. 165 (2): 368-80. -
Barclay, A.N. (1981) The localization of populations of lymphocytes defined by monoclonal antibodies in rat lymphoid tissues.
Immunology. 42 (4): 593-600. -
Whiteland, J.L. et al. (1995) Immunohistochemical detection of T-cell subsets and other leukocytes in paraffin-embedded rat and mouse tissues with monoclonal antibodies.
J Histochem Cytochem. 43 (3): 313-20. -
Baker, S.C. et al. (2011) Cellular Integration and Vascularisation Promoted by a Resorbable, Particulate-Leached, Cross-Linked Poly(ε-caprolactone) Scaffold.
Macromol Biosci. 11: 618-27. -
Romani, P. et al. (2009) Cell survival and polarity of Drosophila follicle cells require the activity of ecdysone receptor B1 isoform.
Genetics. 181: 165-75. -
Stybayeva, G. et al. (2010) Lensfree holographic imaging of antibody microarrays for high-throughput detection of leukocyte numbers and function.
Anal Chem. 82: 3736-44. -
Bastock, R. et al. (2003) Strabismus is asymmetrically localised and binds to Prickle and Dishevelled during Drosophila planar polarity patterning.
Development. 130: 3007-14. -
Brückner, K. et al. (2000) Glycosyltransferase activity of Fringe modulates Notch-Delta interactions.
Nature. 406: 411-5. -
Liversidge, J. et al. (2002) Nitric oxide mediates apoptosis through formation of peroxynitrite and Fas/Fas-ligand interactions in experimental autoimmune uveitis.
Am J Pathol. 160: 905-16. -
Sarpal, R. et al. (2012) Mutational analysis supports a core role for Drosophila α-catenin in adherens junction function.
J Cell Sci. 125: 233-45. -
Zhang, H. et al. (2011) Basic residues in the T-cell receptor ζ cytoplasmic domain mediate membrane association and modulate signaling.
Proc Natl Acad Sci U S A. 108: 19323-8. -
Heck, B.W. et al. (2012) The transcriptional corepressor SMRTER influences both Notch and ecdysone signaling during Drosophila development.
Biol Open. 1 (3): 182-96. -
Clark, I.B. et al. (2011) Fibroblast growth factor signalling controls successive cell behaviours during mesoderm layer formation in Drosophila.
Development. 138: 2705-15. -
Domanitskaya, E. and Schüpbach, T. (2012) CoREST acts as a positive regulator of Notch signaling in the follicle cells of Drosophila melanogaster.
J Cell Sci. 125: 399-410. -
Dragovic, R.A. et al. (2015) Isolation of syncytiotrophoblast microvesicles and exosomes and their characterisation by multicolour flow cytometry and fluorescence Nanoparticle Tracking Analysis.
Methods. 87: 64-74.