CD134 antibody | OX-40

100% Secure


Mouse anti Rat CD134:FITC

Mouse anti Rat CD134

Product Type
Monoclonal Antibody
Clone
OX-40
Isotype
IgG2b
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA730F F 0.1 mg
MCA730GA C F IP 0.1 mg
Mouse anti Rat CD134 antibody, clone OX-40 recognizes the OX40 antigen which is present on activated T lymphocytes that also express CD4 (Paterson et al. 1987).

The antigen is a glycoprotein of approximately 50 kDa which is related in sequence to the low affinity Nerve Growth Factor Receptor (Mallett et al. 1990).

Mouse anti Rat CD134 antibody, clone OX-40 is not mitogenic, but does enhance some T cell responses (Paterson et al. 1987).

Product Details

Target Species
Rat
Product Form
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
Preservative Stabilisers
0.09%Sodium Azide
Carrier Free
Yes
Immunogen
Phytohemagglutinin (PHA) activated Rat Lymph Node Cells.
Approx. Protein Concentrations
IgG concentration 0.1 mg/ml
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells from the NS0/1 Ag4.1 mouse myeloma cell line.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.

Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Shelf Life
18 months from date of despatch.

More Information

UniProt
P15725 Related reagents
Entrez Gene
Tnfrsf4 Related reagents
GO Terms
GO:0016021 integral to membrane
GO:0005031 tumor necrosis factor receptor activity
GO:0006968 cellular defense response
GO:0042098 T cell proliferation
Regulatory
For research purposes only

Applications of CD134 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat
Flow Cytometry 1/10 1/50
Immunohistology - Frozen
Immunoprecipitation
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Human anti Mouse IgG2b:FITC HCA038F 0.1 mg F
Human anti Mouse IgG2b:HRP HCA038P 0.1 mg E
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A 0.5 mg E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed) STAR117D549GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed) STAR117D649GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA 0.1 mg F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F 0.5 mg F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P 0.5 mg E WB
Goat anti Mouse IgG (Fc):FITC STAR120F 1 mg C F
Goat anti Mouse IgG (Fc):HRP STAR120P 1 mg E WB
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A 1 ml F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B 1 mg C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 0.5 mg F
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 1 ml F
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 0.5 mg C E P
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A 1 mg C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P 1 mg E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA 0.1 mg F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B 1 mg F

Negative Isotype Controls Available

Description Product Code Pack Size Applications List Price Quantity
Mouse IgG2b Negative Control:FITC MCA691F 100 Tests F
Mouse IgG2b Negative Control MCA691 100 Tests F

Application Based External Images

Flow Cytometry

Product Specific References

References for CD134 antibody

  1. Paterson, D.J. et al. (1987) Antigens of activated rat T lymphocytes including a molecule of 50,000 Mr detected only on CD4 positive T blasts.
    Mol Immunol. 24 (12): 1281-90.
  2. Mallett, S. et al. (1990) Characterization of the MRC OX40 antigen of activated CD4 positive T lymphocytes--a molecule related to nerve growth factor receptor.
    EMBO J. 9 (4): 1063-8.
  3. Richardson, P.R. et al. (1996) Immunocytochemical study of retinal diode laser photocoagulation in the rat.
    Br J Ophthalmol. 80 (12): 1092-8.
  4. Cousins, L. et al. (2006) Eosinophilic bowel disease controlled by the BB rat-derived lymphopenia/Gimap5 gene.
    Gastroenterology. 131 (5): 1475-85.
  5. Snelgrove RJ et al. (2012) OX40 ligand fusion protein delivered simultaneously with the BCG vaccine provides superior protection against murine Mycobacterium tuberculosis infection.
    J Infect Dis. 205 (6): 975-83.
  6. Stephens, L.A. et al. (2004) Phenotypic characterization of regulatory CD4+CD25+ T cells in rats.
    Int Immunol. 16: 365-75.
  7. Volovitz, I. et al. (2010) T cell vaccination induces the elimination of EAE effector T cells: analysis using GFP-transduced, encephalitogenic T cells.
    J Autoimmun. 35 (2): 135-44.
  8. Stephens, L.A. et al. (2004) Phenotypic characterization of regulatory CD4+CD25+ T cells in rats.
    Int Immunol. 16: 365-75.
  9. Giorgini, A. and Noble, A. (2007) Blockade of chronic graft-versus-host disease by alloantigen-induced CD4+CD25+Foxp3+ regulatory T cells in nonlymphopenic hosts.
    J Leukoc Biol. 82: 1053-61.
  10. Albillos, A. et al. (2010) The biological response modifier AM3 attenuates the inflammatory cell response and hepatic fibrosis in rats with biliary cirrhosis.
    Gut. 59: 943-52.
  11. Humphreys, I.R. et al. (2003) A critical role for OX40 in T cell-mediated immunopathology during lung viral infection.
    J Exp Med. 198: 1237-42.
  12. Walker, L.S. et al. (1999) Compromised OX40 function in CD28-deficient mice is linked with failure to develop CXC chemokine receptor 5-positive CD4 cells and germinal centers.
    J Exp Med. 190: 1115-22.
  13. Thaunat, O. et al. (2010) Immune responses elicited in tertiary lymphoid tissues display distinctive features.
    PLoS One. 5: e11398.
  14. Lühder, F. et al. (2017) Laquinimod enhances central nervous system barrier functions.
    Neurobiol Dis. Feb 21. pii: S0969-9961(17)30031-1. [Epub ahead of print]