CD134 antibody | OX-40
Mouse anti Rat CD134
- Product Type
- Monoclonal Antibody
- Clone
- OX-40
- Isotype
- IgG2b
- Specificity
- CD134
Antibody Quality and Performance Guarantee
| Mouse anti Rat CD134 antibody, clone OX-40 recognizes the OX40 antigen which is present on activated T lymphocytes that also express CD4 (Paterson et al. 1987). The antigen is a glycoprotein of approximately 50 kDa which is related in sequence to the low affinity Nerve Growth Factor Receptor (Mallett et al. 1990). Mouse anti Rat CD134 antibody, clone OX-40 is not mitogenic, but does enhance some T cell responses (Paterson et al. 1987). |
- Target Species
- Rat
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide - Carrier Free
- Yes
- Immunogen
- Phytohemagglutinin (PHA) activated Rat Lymph Node Cells.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunized BALB/c mice were fused with cells from the NS0/1 Ag4.1 mouse myeloma cell line.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
1/10 | 1/50 |
| Immunohistology - Frozen | |
||
| Immunoprecipitation | |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Mouse IgG2b Negative Control | MCA691 | F | 100 Tests |
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| List Price | Your Price | ||||||
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| Description | Mouse IgG2b Negative Control | ||||||
References for CD134 antibody
-
Paterson, D.J. et al. (1987) Antigens of activated rat T lymphocytes including a molecule of 50,000 Mr detected only on CD4 positive T blasts.
Mol Immunol. 24 (12): 1281-90. -
Mallett, S. et al. (1990) Characterization of the MRC OX40 antigen of activated CD4 positive T lymphocytes--a molecule related to nerve growth factor receptor.
EMBO J. 9 (4): 1063-8. -
Richardson, P.R. et al. (1996) Immunocytochemical study of retinal diode laser photocoagulation in the rat.
Br J Ophthalmol. 80 (12): 1092-8. -
Cousins, L. et al. (2006) Eosinophilic bowel disease controlled by the BB rat-derived lymphopenia/Gimap5 gene.
Gastroenterology. 131 (5): 1475-85. -
Snelgrove RJ et al. (2012) OX40 ligand fusion protein delivered simultaneously with the BCG vaccine provides superior protection against murine Mycobacterium tuberculosis infection.
J Infect Dis. 205 (6): 975-83. -
Volovitz, I. et al. (2010) T cell vaccination induces the elimination of EAE effector T cells: analysis using GFP-transduced, encephalitogenic T cells.
J Autoimmun. 35 (2): 135-44. -
Stephens, L.A. et al. (2004) Phenotypic characterization of regulatory CD4+CD25+ T cells in rats.
Int Immunol. 16: 365-75. -
Giorgini, A. and Noble, A. (2007) Blockade of chronic graft-versus-host disease by alloantigen-induced CD4+CD25+Foxp3+ regulatory T cells in nonlymphopenic hosts.
J Leukoc Biol. 82: 1053-61.
View The Latest Product References
-
Albillos, A. et al. (2010) The biological response modifier AM3 attenuates the inflammatory cell response and hepatic fibrosis in rats with biliary cirrhosis.
Gut. 59: 943-52. -
Humphreys, I.R. et al. (2003) A critical role for OX40 in T cell-mediated immunopathology during lung viral infection.
J Exp Med. 198: 1237-42. -
Walker, L.S. et al. (1999) Compromised OX40 function in CD28-deficient mice is linked with failure to develop CXC chemokine receptor 5-positive CD4 cells and germinal centers.
J Exp Med. 190: 1115-22. -
Thaunat, O. et al. (2010) Immune responses elicited in tertiary lymphoid tissues display distinctive features.
PLoS One. 5: e11398. -
Lühder, F. et al. (2017) Laquinimod enhances central nervous system barrier functions.
Neurobiol Dis. 102: 60-69. -
Cheng, H.Y. et al. (2018) Bioimaging of alloantigen-stimulated regulatory T cells in rat vascularized composite allotransplantation.
PLoS One. 13 (9): e0203624. -
Lodygin, D. et al. (2019) β-Synuclein-reactive T cells induce autoimmune CNS grey matter degeneration.
Nature. 566 (7745): 503-8.
- Synonyms
- OX40
- RRID
- AB_2207361
- UniProt
- P15725
- Entrez Gene
- Tnfrsf4
- GO Terms
- GO:0016021 integral to membrane
- GO:0005031 tumor necrosis factor receptor activity
- GO:0006968 cellular defense response
- GO:0042098 T cell proliferation
MCA730GA
156509
1609
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