CD4 antibody | KEN-4




Mouse anti Rabbit CD4 antibody, clone KEN-4 blocks the allogeneic mixed lymphocyte reaction response.
Product Details
- Target Species
- Rabbit
- Species Cross-Reactivity
-
Target Species Cross Reactivity Brown Hare (Lepus europeus) - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin - Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Carrier Free
- Yes
- Immunogen
- Rabbit thymocytes.
- Approx. Protein Concentrations
- IgG concentration 0.1 mg/ml
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunized mice were fused with cells of the mouse PAI myeloma cell line.
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC or -20oC if preferred.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P46630
- Entrez Gene
- CD4
- GO Terms
- GO:0007155 cell adhesion
- GO:0016021 integral to membrane
- GO:0006955 immune response
- GO:0045058 T cell selection
- Regulatory
- For research purposes only
Applications of CD4 antibody
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
Flow Cytometry | Neat | ||
Flow Cytometry | 1/25 | 1/200 | |
Immunohistology - Frozen | |||
Immunoprecipitation |
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells or 100ul whole blood.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells or 100ul whole blood.
Secondary Antibodies Available
Negative Isotype Controls Available
Description | Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|---|
Mouse IgG2a Negative Control:FITC | MCA929F | F | 100 Tests |
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Product Specific References
References for CD4 antibody
-
Kotani, M. et al. (1993) Generation and characterization of monoclonal antibodies against rabbit CD4, CD5 and CD11a antigens.
J Immunol Methods. 157 (1-2): 241-52. -
Dewals, B. et al. (2008) Malignant catarrhal fever induced by alcelaphine herpesvirus 1 is associated with proliferation of CD8+ T cells supporting a latent infection.
PLos ONE 3: e1627. -
Chentoufi, A.A. et al. (2010) A novel HLA (HLA-A*0201) transgenic rabbit model for preclinical evaluation of human CD8+ T cell epitope-based vaccines against ocular herpes.
J Immunol. 184: 2561-71. -
Perosa, F. and Dammacco, F. (1994) Anti-idiotypic monoclonal antibodies (mAb) to an anti-CD4 mAb induce CD4+ T cell depletion in rabbit.
Int J Clin Lab Res. 24: 208-12. -
Khan, A.A. et al. (2015) Therapeutic immunization with a mixture of herpes simplex virus 1 glycoprotein D-derived “asymptomatic” human CD8+ T-cell epitopes decreases spontaneous ocular shedding in latently infected HLA transgenic rabbits: association with low frequency of local PD-1+ TIM-3+ CD8+ exhausted T cells.
J Virol. 89 (13): 6619-32. -
Rütgen, B.C. et al. (2014) Exploratory assessment of CD4+ T lymphocytes in brown hares (Lepus europeus) using a cross-reactive anti-rabbit CD4 antibody.
Vet Immunol Immunopathol. 161 (1-2): 108-15. -
Boutard, B. et al. (2015) The α2,3-sialyltransferase encoded by myxoma virus is a virulence factor that contributes to immunosuppression.
PLoS One. 10 (2): e0118806. -
Pakandl, M. et al. (2008) Dependence of the immune response to coccidiosis on the age of rabbit suckling.
Parasitol Res. 103 (6): 1265-71. -
Renaux, S. et al. (2003) Dynamics and responsiveness of T-lymphocytes in secondary lymphoid organs of rabbits developing immunity to Eimeria intestinalis.
Vet Parasitol. 110 (3-4): 181-95. -
Yang, J. et al. (2009) Expression and localization of rabbit B-cell activating factor (BAFF) and its specific receptor BR3 in cells and tissues of the rabbit immune system.
Dev Comp Immunol. 33 (5): 697-708. -
Beghelli, D et al. (2016) Effects of Oregano (Origanum vulgare L.) and Rosemary (Rosmarinus officinalis L.) Aqueous Extracts On in vitro Rabbit Immune Responses
MOJ Immunology. 4 (4) [Epub ahead of print]. -
Sorel, O. et al. (2017) Macavirus latency-associated protein evades immune detection through regulation of protein synthesis in cis depending upon its glycin/glutamate-rich domain.
PLoS Pathog. 13 (10): e1006691. -
Myster, F. et al. (2015) Viral semaphorin inhibits dendritic cell phagocytosis and migration but is not essential for gammaherpesvirus-induced lymphoproliferation in malignant catarrhal fever.
J Virol. 89 (7): 3630-47. -
Penadés, M. et al. (2018) Long-term implications of feed energy source in different genetic types of reproductive rabbit females. II. Immunologic status.
Animal. 12 (9): 1877-85. -
Jeklova, E. et al. (2020) Characterization of humoral and cell-mediated immunity in rabbits orally infected with Encephalitozoon cuniculi..
Vet Res. 51 (1): 79.
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