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SWC5 antibody | b37c10

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Anti Pig SWC5 Antibody, clone b37c10 thumbnail image 1 Anti Pig SWC5 Antibody, clone b37c10 thumbnail image 2 Anti Pig SWC5 Antibody, clone b37c10 thumbnail image 3
Anti Pig SWC5 Antibody, clone b37c10 gallery image 1
Figure A. FITC conjugated Mouse anti Pig CD5 (MCA2307F) and Mouse IgG1 isotype control (MCA928) detected with Goat anti Mouse IgG1:RPE (STAR132PE). Figure B. FITC conjugated Mouse anti Pig CD5 (MCA2307F) and Mouse anti Pig SWC5 (MCA6050GA) detected with Goat anti Mouse IgG1:RPE (STAR132PE). All experiments performed on red cell lysed porcine blood gated on lymphocytes in the presence of 10% pig serum. Data acquired on the ZE5™ Cell Analyzer.
Anti Pig SWC5 Antibody, clone b37c10 gallery image 2
Figure A. FITC conjugated Mouse anti Pig CD3 (MCA5951F) and Alexa Fluor 647 conjugated Mouse IgG1 isotype control (MCA928A647). Figure B. FITC conjugated Mouse anti Pig CD3 (MCA5951F) and Alexa Fluor 647 conjugated Mouse anti Pig SWC5 (MCA6050A647). All experiments performed on red cell lysed porcine blood gated on lymphocytes in the presence of 10% pig serum. Data acquired on the ZE5™ Cell Analyzer.
Anti Pig SWC5 Antibody, clone b37c10 gallery image 3
Figure A. FITC conjugated Mouse anti Pig CD3 (MCA5951F) and RPE conjugated Mouse IgG1 isotype control (MCA928PE). Figure B. FITC conjugated Mouse anti Pig CD3 (MCA5951F) and RPE conjugated Mouse anti Pig SWC5 (MCA6050PE). All experiments performed on red cell lysed porcine blood gated on lymphocytes in the presence of 10% pig serum. Data acquired on the ZE5™ Cell Analyzer.

Mouse anti Pig SWC5:Alexa Fluor® 647

Mouse anti Pig SWC5

Mouse anti Pig SWC5:RPE

Product Type
Monoclonal Antibody
Clone
b37c10
Isotype
IgG1
Product Code Applications Pack Size List Price Quantity
MCA6050A647
Datasheet
F
SDS
100 Tests/1ml loader

MCA6050GA
Datasheet
F
SDS
0.1 mg loader

MCA6050PE
Datasheet
F
SDS
100 Tests loader

Mouse anti Pig SWC5 antibody, clone b37c10 recognizes the porcine SWC5 cell surface antigen. SWC5 has a molecular weight of 180 kDa and is expressed only on subset of γ/δ T cells.
SWC5 and CD2 expression divides γδ T cells into three subpopulations: CD2-SWC5-, CD2-SWC5+ and CD2+SWC5- γδ T cells subsets. The vast majority of CD2-SWC5- and CD2-SWC5+ T cells are negative for CD8α and SLA-DR and positive for CD27. Only thymus and spleen has an extra CD2+SWC5+ population.

Product Details

Target Species
Pig
Product Form
Purified IgG conjugated to Alexa Fluor® 647 - liquid
Product Form
Purified IgG - liquid
Product Form
Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
Reconstitution
Reconstitute with 1.0 ml distilled water
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
5% Sucrose
Carrier Free
Yes
Immunogen
Porcine T cells
Approx. Protein Concentrations
IgG concentration 0.05 mg/ml
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light.
Guarantee
12 months from date of despatch
Guarantee
12 months from date of despatch
Guarantee
12 months from date of despatch

More Information

Acknowledgements
This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchased product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
Regulatory
For research purposes only

Applications of SWC5 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat 1/10
Flow Cytometry 1/10 1/200
Flow Cytometry Neat 1/10
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A E WB 0.5 mg loader
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F F 0.5 mg loader
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P E WB 0.5 mg loader
Goat anti Mouse IgG (Fc):FITC STAR120F C F 1 mg loader
Goat anti Mouse IgG (Fc):HRP STAR120P E WB 1 mg loader
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 F 0.5 mg loader
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 C E P 0.5 mg loader
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A C E WB 1 mg loader
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P E 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA F IF WB 0.1 mg loader
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B F 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B C E P RE WB 1 mg loader
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA F IF WB 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA F IF 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA F WB 0.1 mg loader
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A F 1 ml loader
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 F 1 ml loader

Negative Isotype Controls Available

Description Product Code Applications Pack Size List Price Quantity
Mouse IgG1 Negative Control:Alexa Fluor® 647 MCA928A647 F 100 Tests/1ml loader
Mouse IgG1 Negative Control MCA928 F 100 Tests loader
Mouse IgG1 Negative Control:RPE MCA928PE F 100 Tests loader

Product Specific References

References for SWC5 antibody

  1. Binns, R.M. et al. (1992) Subsets of null and gamma delta T-cell receptor+ T lymphocytes in the blood of young pigs identified by specific monoclonal antibodies.
    Immunology. 77 (2): 219-27.
  2. Binns, R.M. (1994) The Null/gamma delta TCR+ T cell family in the pig.
    Vet Immunol Immunopathol. 43 (1-3): 69-77.
  3. Sedlak, C. et al. (2014) CD2 and CD8α define porcine γδ T cells with distinct cytokine production profiles.
    Dev Comp Immunol. 45 (1): 97-106.

Further Reading

  1. Piriou-guzylack, L. & Salmon, H. (2008) Membrane markers of the immune cells in swine: an update.
    Vet Res. 39 (6): 54.

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Request a different product with the SWC5 specificity