Browsing Modes for Business Users

SLA Class II DR antibody | 2E9/13

ISO 9001 Quality Assurance Manufacturing | Print
Anti Pig SLA Class II DR Antibody, clone 2E9/13 thumbnail image 1 Anti Pig SLA Class II DR Antibody, clone 2E9/13 thumbnail image 2 Anti Pig SLA Class II DR Antibody, clone 2E9/13 thumbnail image 3 Anti Pig SLA Class II DR Antibody, clone 2E9/13 thumbnail image 4
Anti Pig SLA Class II DR Antibody, clone 2E9/13 gallery image 1
Pig peripheral blood lymphocytes stained with Mouse anti Pig SLA Class II DR (MCA2314GA) followed by FITC conjugated Goat anti Mouse IgG antibody (STAR70)
Anti Pig SLA Class II DR Antibody, clone 2E9/13 gallery image 2
Published customer image:
Mouse anti Pig SLA Class II DR antibody, clone 2E9/13 (MCA2314GA) used for the evaluation of MHC expression by immunohistochemistry on formalin fixed, paraffin embedded tissue sections.
Image caption:
Analysis of decellularized liver materials.
(a,b) Representative H&E staining of the ultrastructure of native liver and decellularized liver matrix. Scale bars = 100 μm. DAPI staining (c,d) and Sirius Red staining (e,f) illustrate a lack of residual cellular components and the preservation of matrix components in the decellularized ECM. Scale bars = 200 μm. Immunohistochemical staining of native and decellularized liver tissues for α-Gal (g,h), SLA-2 (i,j), SLA-DRα (k,l) are shown. Scale bars = 100 μm. (m) Semi-quantitative PCR for the α-Gal, SLA-DRα, PERV, and SLA-2 antigens in the liver scaffolds. Na = native liver; De = decellularized liver matrix.

From: Wang Y, Bao J, Wu X, Wu Q, Li Y, Zhou Y, Li L, Bu H.
Genipin crosslinking reduced the immunogenicity of xenogeneic decellularized porcine whole-liver matrices through regulation of immune cell proliferation and polarization.
Sci Rep. 2016 Apr 21;6:24779.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Anti Pig SLA Class II DR Antibody, clone 2E9/13 gallery image 3
Published customer image:
Mouse anti Porcine SLA II DR antibody, clone 2E9/13 (MCA2314GA) used for the evaluation of SLA class II expression on purified B cells in vitro over time by flow cytometry
Image caption:
CD40L induces B cell activation.
CD21+ purified B cell populations were cultured in the presence or absence of CD40L (1.0 μg/ml), gated on live cells, and then analyzed for changes in the median fluorescence intensity (MFI) of SLA-DR (A) and CD80 (B). Cells were analyzed prior to culture and at 24 h intervals for a period of 7 days. One representative experiment of two performed is displayed. Data shown are mean ± SEM. Statistical differences of *p ≤0.05, ** p ≤0.01, ***p ≤0.001 are indicated.

From: Rahe MC, Murtaugh MP (2017)
Interleukin-21 Drives Proliferation and Differentiation of Porcine Memory B Cells into Antibody Secreting Cells.
PLoS ONE 12(1): e0171171.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Anti Pig SLA Class II DR Antibody, clone 2E9/13 gallery image 4
Published customer image:
Mouse anti Pig SLA Class II DR antibody, clone 2E9/13 (MCA2314GA) used to evaluate SLA II expression on porcine dendritic cells by flow cytometry.
Image caption:
Dot plots and percentage of dendritic cells expressing surface markers. Flow cytometric analysis was conducted to detect double-positive staining for surface markers (A: CD1a and SWC3a; B: MHC-II and CD80/86; a and b: background control).

From: Liu S, Li Q, Qiao J, Wang J, Cui D, Gu K, Zhou S, Li H.
Endothelial IL-8 induced by porcine circovirus type 2 affects dendritic cell maturation and antigen-presenting function.
Virol J. 2019 Dec 12;16(1):154.
doi: 10.1186/s12985-019-1256-z.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.

Filter by Application:

F P

Mouse anti Pig SLA Class II DR

Product Type
Monoclonal Antibody
Clone
2E9/13
Isotype
IgG2b
Specificity
SLA Class II DR

Quick Links:

 Antibody Quality and Performance Guarantee
 Unrivaled Technical Support
Product Code Applications Pack Size List Price Your Price Qty
MCA2314GA
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C F IP 0.1 mg loader
List Price Your Price
loader

Mouse anti Pig SLA Class II DR antibody, clone 2E9/13 recognizes SLA DR molecules which are expressed on all B cells, antigen presenting cells and on certain subsets of resting and activated T cells. Mouse anti Pig SLA Class II DR antibody, clone 289/13 reacts with lymphocytes from all outbred and miniature pigs so far tested, suggesting that it recognizes a monomorphic determinant of porcine SLA DR.

The major histocompatibility complex (MHC) is a cluster of genes that are important in the immune response to infections. In pigs, this is referred to as the swine leukocyte antigen (SLA) region. There are 3 major MHC class II proteins encoded by the SLA which are SLA DP, SLA DQ and SLA DR.

Mouse anti pig SLA class II DR, clone 2E9/13 immunoprecipitates a heterodimer composed of two polypeptides of ~28 and ~35 kDa from NP-40 extracts of biotin surface-labeled porcine peripheral blood mononuclear cells. Mouse anti Pig SLA Class II DR antibody, clone 289/13 is reported to inhibit the mixed lymphocyte reaction and T cell stimulation induced by African swine fever virus and staphylococcal enterotoxin B (Bullido et al. 1997).

Target Species
Pig
Species Cross-Reactivity
Target SpeciesCross Reactivity
Bovine
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% sodium azide (NaN3)
Carrier Free
Yes
Immunogen
Porcine monocytes.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the mouse X63-Ag.8.653 myeloma cell line.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/25 1/200
Immunohistology - Frozen
Immunoprecipitation
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10μl of the suggested working dilution to 1x106 cells in 100μl

Description Product Code Applications Pack Size List Price Your Price Quantity
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A E WB 0.5 mg
List Price Your Price
Description Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA F IF 0.1 mg
List Price Your Price
Description Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):DyLight®550 (Multi Species Adsorbed) STAR117D550 F IF WB 0.1 mg
List Price Your Price
Description Goat anti Mouse IgG (H/L):DyLight®550 (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):DyLight®650 (Multi Species Adsorbed) STAR117D650 F IF 0.1 mg
List Price Your Price
Description Goat anti Mouse IgG (H/L):DyLight®650 (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA F WB 0.1 mg
List Price Your Price
Description Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA F IF WB 0.1 mg
List Price Your Price
Description Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F F 0.5 mg
List Price Your Price
Description Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P C E WB 0.5 mg
List Price Your Price
Description Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)
Goat anti Mouse IgG (Fc):FITC STAR120F C F 1 mg
List Price Your Price
Description Goat anti Mouse IgG (Fc):FITC
Goat anti Mouse IgG (Fc):HRP STAR120P E WB 1 mg
List Price Your Price
Description Goat anti Mouse IgG (Fc):HRP
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A F 1 ml
List Price Your Price
Description Rabbit F(ab')2 anti Mouse IgG:RPE
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B C E P RE WB 1 mg loader
List Price Your Price
loader
Description Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 F 0.5 mg
List Price Your Price
Description Goat anti Mouse IgG:FITC (Rat Adsorbed)
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 F 1 ml
List Price Your Price
Description Goat anti Mouse IgG:RPE (Rat Adsorbed)
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 C E P 0.5 mg
List Price Your Price
Description Goat anti Mouse IgG:HRP (Rat Adsorbed)
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A C E WB 1 mg
List Price Your Price
Description Goat anti Mouse IgG/A/M:Alk. Phos.
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P E 1 mg
List Price Your Price
Description Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B F 1 mg loader
List Price Your Price
loader
Description Rabbit F(ab')2 anti Mouse IgG:FITC

Description Product Code Applications Pack Size List Price Your Price Quantity
Mouse IgG2b Negative Control MCA691 F 100 Tests loader
List Price Your Price
loader
Description Mouse IgG2b Negative Control

References for SLA Class II DR antibody

  1. Bullido, R. et al. (1997) Characterization of five monoclonal antibodies specific for swine class II major histocompatibility antigens and crossreactivity studies with leukocytes of domestic animals.
    Dev Comp Immunol. 21 (3): 311-22.
  2. Jeong, H.J. et al. (2010) Comparative measurement of cell-mediated immune responses of swine to the M and N proteins of porcine reproductive and respiratory syndrome virus.
    Clin Vaccine Immunol. 17: 503-12.
  3. Ding, Q. et al. (2011) Human PD-L1-overexpressing porcine vascular endothelial cells induce functionally suppressive human CD4+CD25hiFoxp3+ Treg cells.
    J Leukoc Biol. 90 (1): 77-86.
  4. Iwase H et al. (2015) Initial in vivo experience of pig artery patch transplantation in baboons using mutant MHC (CIITA-DN) pigs.
    Transpl Immunol. 32 (2): 99-108.
  5. Zanotti, C. et al. (2015) Differential Biological Activities of Swine Interferon-α Subtypes.
    J Interferon Cytokine Res. 35 (12): 990-1002.
  6. Gardner, D.S. et al. (2016) Remote effects of acute kidney injury in a porcine model.
    Am J Physiol Renal Physiol. 310 (4): F259-71.
  7. Wang, Y. et al. (2016) Genipin crosslinking reduced the immunogenicity of xenogeneic decellularized porcine whole-liver matrices through regulation of immune cell proliferation and polarization.
    Sci Rep. 6: 24779.
  8. Singleton, H. et al. (2016) Establishing Porcine Monocyte-Derived Macrophage and Dendritic Cell Systems for Studying the Interaction with PRRSV-1.
    Front Microbiol. 7: 832.
    9. View The Latest Product References
  9. Mašek, J. et al. (2017) Multi-layered nanofibrous mucoadhesive films for buccal and sublingual administration of drug-delivery and vaccination nanoparticles - important step towards effective mucosal vaccines.
    J Control Release. 249: 183-95.
  10. Rahe, M.C. & Murtaugh, M.P. (2017) Interleukin-21 Drives Proliferation and Differentiation of Porcine Memory B Cells into Antibody Secreting Cells.
    PLoS One. 12 (1): e0171171.
  11. Yang, N. et al. (2018) Reduced antigen presentation capability and modified inflammatory/immunosuppressive cytokine expression of induced monocyte-derived dendritic cells from peripheral blood of piglets infected with porcine circovirus type 2.
    Arch Virol. 163 (5): 1231-9.
  12. López, E. et al. (2019) Identification of very early inflammatory markers in a porcine myocardial infarction model.
    BMC Vet Res. 15 (1): 91.
  13. Liu, S. et al. (2019) Endothelial IL-8 induced by porcine circovirus type 2 affects dendritic cell maturation and antigen-presenting function.
    Virol J. 16 (1): 154.
  14. Radlowski, E.C. et al. (2021) Combination-Feeding Causes Differences in Aspects of Systemic and Mucosal Immune Cell Phenotypes and Functions Compared to Exclusive Sow-Rearing or Formula-Feeding in Piglets.
    Nutrients. 13(4):1097.
  15. Franzoni, G. et al. (2022) Analyses of the Impact of Immunosuppressive Cytokines on Porcine Macrophage Responses and Susceptibility to Infection to African Swine Fever Viruses.
    Pathogens. 11 (2): 166.
  16. Arenal, Á. et al. (2022) Effects of Cardiac Stem Cell on Postinfarction Arrhythmogenic Substrate.
    Int J Mol Sci. 23 (24): 16211.

Further Reading

  1. Piriou-Guzylack, L. (2008) Membrane markers of the immune cells in swine: an update.
    Vet Res. 39: 54.
  2. Rayat GR et al. (2016) First update of the International Xenotransplantation Association consensus statement on conditions for undertaking clinical trials of porcine islet products in type 1 diabetes - Chapter 3: Porcine islet product manufacturing and release testing criteria.
    Xenotransplantation. 23 (1): 38-45.

Flow Cytometry

UniProt
Q85ZW4

MCA2314GA

147544 154296 161441

If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.

View more products with SLA CLASS specificity

Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

View all Anti-Pig Products
Please note