SLA Class II DR antibody | 2E9/13



Product Details
- Target Species
- Pig
- Species Cross-Reactivity
-
Target Species Cross Reactivity Bovine - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin - Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Carrier Free
- Yes
- Approx. Protein Concentrations
- IgG concentration 0.1 mg/ml
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
More Information
- Regulatory
- For research purposes only
Applications of SLA Class II DR antibody
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
Flow Cytometry | Neat | ||
Flow Cytometry | 1/25 | 1/200 | |
Immunohistology - Frozen | |||
Immunoprecipitation |
- Flow Cytometry
- Use 10ul of the suggested working dilution to 1x106 cells in 100ul.
- Flow Cytometry
- Use 10ul of the suggested working dilution to 1x106 cells in 100ul.
Secondary Antibodies Available
Negative Isotype Controls Available
Description | Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|---|
Mouse IgG2b Negative Control:FITC | MCA691F | F | 100 Tests |
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|
Mouse IgG2b Negative Control | MCA691 | F | 100 Tests |
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Product Specific References
References for SLA Class II DR antibody
-
Bullido, R. et al. (1997) Characterization of five monoclonal antibodies specific for swine class II major histocompatibility antigens and crossreactivity studies with leukocytes of domestic animals.
Dev Comp Immunol. 21 (3): 311-22. -
Jeong, H.J. et al. (2010) Comparative measurement of cell-mediated immune responses of swine to the M and N proteins of porcine reproductive and respiratory syndrome virus.
Clin Vaccine Immunol. 17: 503-12. -
Ding, Q. et al. (2011) Human PD-L1-overexpressing porcine vascular endothelial cells induce functionally suppressive human CD4+CD25hiFoxp3+ Treg cells.
J Leukoc Biol. 90 (1): 77-86. -
Wang, Y. et al. (2016) Genipin crosslinking reduced the immunogenicity of xenogeneic decellularized porcine whole-liver matrices through regulation of immune cell proliferation and polarization.
Sci Rep. 6: 24779. -
Park KM et al. (2013) Generation of porcine induced pluripotent stem cells and evaluation of their major histocompatibility complex protein expression in vitro.
Vet Res Commun. 37 (4): 293-301. -
Iwase H et al. (2015) Initial in vivo experience of pig artery patch transplantation in baboons using mutant MHC (CIITA-DN) pigs.
Transpl Immunol. 32 (2): 99-108. -
Singleton, H. et al. (2016) Establishing Porcine Monocyte-Derived Macrophage and Dendritic Cell Systems for Studying the Interaction with PRRSV-1.
Front Microbiol. 7: 832. -
Zanotti, C. et al. (2015) Differential Biological Activities of Swine Interferon-α Subtypes.
J Interferon Cytokine Res. 35 (12): 990-1002. -
Rayat GR et al. (2016) First update of the International Xenotransplantation Association consensus statement on conditions for undertaking clinical trials of porcine islet products in type 1 diabetes - Chapter 3: Porcine islet product manufacturing and release testing criteria.
Xenotransplantation. 23 (1): 38-45. -
Mašek J et al. (2016) Multi-layered nanofibrous mucoadhesive films for buccal and sublingual administration of drug-delivery and vaccination nanoparticles - important step towards effective mucosal vaccines.
J Control Release. Jul 25. pii: S0168-3659(16)30471-0 [Epub ahead of print] -
Gardner, D.S. et al. (2016) Remote effects of acute kidney injury in a porcine model.
Am J Physiol Renal Physiol. 310 (4): F259-71. -
Rahe, M.C. & Murtaugh, M.P. (2017) Interleukin-21 Drives Proliferation and Differentiation of Porcine Memory B Cells into Antibody Secreting Cells.
PLoS One. 12 (1): e0171171. -
López, E. et al. (2019) Identification of very early inflammatory markers in a porcine myocardial infarction model.
BMC Vet Res. 15 (1): 91. -
Yang, N. et al. (2018) Reduced antigen presentation capability and modified inflammatory/immunosuppressive cytokine expression of induced monocyte-derived dendritic cells from peripheral blood of piglets infected with porcine circovirus type 2.
Arch Virol. 163 (5): 1231-9.
Further Reading
-
Piriou-Guzylack, L. (2008) Membrane markers of the immune cells in swine: an update.
Vet Res. 39: 54.
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