SLA Class II DR antibody | 2E9/13
Mouse anti Pig SLA Class II DR antibody, clone 2E9/13 (MCA2314GA) used for the evaluation of MHC expression by immunohistochemistry on formalin fixed, paraffin embedded tissue sections.
Image caption:
Analysis of decellularized liver materials.
(a,b) Representative H&E staining of the ultrastructure of native liver and decellularized liver matrix. Scale bars = 100 μm. DAPI staining (c,d) and Sirius Red staining (e,f) illustrate a lack of residual cellular components and the preservation of matrix components in the decellularized ECM. Scale bars = 200 μm. Immunohistochemical staining of native and decellularized liver tissues for α-Gal (g,h), SLA-2 (i,j), SLA-DRα (k,l) are shown. Scale bars = 100 μm. (m) Semi-quantitative PCR for the α-Gal, SLA-DRα, PERV, and SLA-2 antigens in the liver scaffolds. Na = native liver; De = decellularized liver matrix.
From: Wang Y, Bao J, Wu X, Wu Q, Li Y, Zhou Y, Li L, Bu H.
Genipin crosslinking reduced the immunogenicity of xenogeneic decellularized porcine whole-liver matrices through regulation of immune cell proliferation and polarization.
Sci Rep. 2016 Apr 21;6:24779.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Porcine SLA II DR antibody, clone 2E9/13 (MCA2314GA) used for the evaluation of SLA class II expression on purified B cells in vitro over time by flow cytometry
Image caption:
CD40L induces B cell activation.
CD21+ purified B cell populations were cultured in the presence or absence of CD40L (1.0 μg/ml), gated on live cells, and then analyzed for changes in the median fluorescence intensity (MFI) of SLA-DR (A) and CD80 (B). Cells were analyzed prior to culture and at 24 h intervals for a period of 7 days. One representative experiment of two performed is displayed. Data shown are mean ± SEM. Statistical differences of *p ≤0.05, ** p ≤0.01, ***p ≤0.001 are indicated.
From: Rahe MC, Murtaugh MP (2017)
Interleukin-21 Drives Proliferation and Differentiation of Porcine Memory B Cells into Antibody Secreting Cells.
PLoS ONE 12(1): e0171171.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Pig SLA Class II DR antibody, clone 2E9/13 (MCA2314GA) used to evaluate SLA II expression on porcine dendritic cells by flow cytometry.
Image caption:
Dot plots and percentage of dendritic cells expressing surface markers. Flow cytometric analysis was conducted to detect double-positive staining for surface markers (A: CD1a and SWC3a; B: MHC-II and CD80/86; a and b: background control).
From: Liu S, Li Q, Qiao J, Wang J, Cui D, Gu K, Zhou S, Li H.
Endothelial IL-8 induced by porcine circovirus type 2 affects dendritic cell maturation and antigen-presenting function.
Virol J. 2019 Dec 12;16(1):154.
doi: 10.1186/s12985-019-1256-z.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
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F P ResetMouse anti Pig SLA Class II DR
- Product Type
- Monoclonal Antibody
- Clone
- 2E9/13
- Isotype
- IgG2b
- Specificity
- SLA Class II DR
Quick Links:
Antibody Quality and 
Unrivaled 
| Mouse anti Pig SLA Class II DR antibody, clone 2E9/13 recognizes SLA DR molecules which are expressed on all B cells, antigen presenting cells and on certain subsets of resting and activated T cells. Mouse anti Pig SLA Class II DR antibody, clone 289/13 reacts with lymphocytes from all outbred and miniature pigs so far tested, suggesting that it recognizes a monomorphic determinant of porcine SLA DR. The major histocompatibility complex (MHC) is a cluster of genes that are important in the immune response to infections. In pigs, this is referred to as the swine leukocyte antigen (SLA) region. There are 3 major MHC class II proteins encoded by the SLA which are SLA DP, SLA DQ and SLA DR. Mouse anti pig SLA class II DR, clone 2E9/13 immunoprecipitates a heterodimer composed of two polypeptides of ~28 and ~35 kDa from NP-40 extracts of biotin surface-labeled porcine peripheral blood mononuclear cells. Mouse anti Pig SLA Class II DR antibody, clone 289/13 is reported to inhibit the mixed lymphocyte reaction and T cell stimulation induced by African swine fever virus and staphylococcal enterotoxin B (Bullido et al. 1997). |
- Target Species
- Pig
- Species Cross-Reactivity
-
Target Species Cross Reactivity Bovine - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Carrier Free
- Yes
- Immunogen
- Porcine monocytes.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised BALB/c mice were fused with cells of the mouse X63-Ag.8.653 myeloma cell line.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
1/25 | 1/200 |
| Immunohistology - Frozen | |
||
| Immunoprecipitation | |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to 1x106 cells in 100ul.
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Mouse IgG2b Negative Control | MCA691 | F | 100 Tests |
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| Description | Mouse IgG2b Negative Control | ||||||
References for SLA Class II DR antibody
-
Bullido, R. et al. (1997) Characterization of five monoclonal antibodies specific for swine class II major histocompatibility antigens and crossreactivity studies with leukocytes of domestic animals.
Dev Comp Immunol. 21 (3): 311-22. -
Jeong, H.J. et al. (2010) Comparative measurement of cell-mediated immune responses of swine to the M and N proteins of porcine reproductive and respiratory syndrome virus.
Clin Vaccine Immunol. 17: 503-12. -
Ding, Q. et al. (2011) Human PD-L1-overexpressing porcine vascular endothelial cells induce functionally suppressive human CD4+CD25hiFoxp3+ Treg cells.
J Leukoc Biol. 90 (1): 77-86. -
Wang, Y. et al. (2016) Genipin crosslinking reduced the immunogenicity of xenogeneic decellularized porcine whole-liver matrices through regulation of immune cell proliferation and polarization.
Sci Rep. 6: 24779. -
Park KM et al. (2013) Generation of porcine induced pluripotent stem cells and evaluation of their major histocompatibility complex protein expression in vitro.
Vet Res Commun. 37 (4): 293-301. -
Iwase H et al. (2015) Initial in vivo experience of pig artery patch transplantation in baboons using mutant MHC (CIITA-DN) pigs.
Transpl Immunol. 32 (2): 99-108. -
Singleton, H. et al. (2016) Establishing Porcine Monocyte-Derived Macrophage and Dendritic Cell Systems for Studying the Interaction with PRRSV-1.
Front Microbiol. 7: 832. -
Zanotti, C. et al. (2015) Differential Biological Activities of Swine Interferon-α Subtypes.
J Interferon Cytokine Res. 35 (12): 990-1002.
View The Latest Product References
-
Rayat GR et al. (2016) First update of the International Xenotransplantation Association consensus statement on conditions for undertaking clinical trials of porcine islet products in type 1 diabetes - Chapter 3: Porcine islet product manufacturing and release testing criteria.
Xenotransplantation. 23 (1): 38-45. -
Mašek J et al. (2016) Multi-layered nanofibrous mucoadhesive films for buccal and sublingual administration of drug-delivery and vaccination nanoparticles - important step towards effective mucosal vaccines.
J Control Release. Jul 25. pii: S0168-3659(16)30471-0 [Epub ahead of print] -
Gardner, D.S. et al. (2016) Remote effects of acute kidney injury in a porcine model.
Am J Physiol Renal Physiol. 310 (4): F259-71. -
Rahe, M.C. & Murtaugh, M.P. (2017) Interleukin-21 Drives Proliferation and Differentiation of Porcine Memory B Cells into Antibody Secreting Cells.
PLoS One. 12 (1): e0171171. -
López, E. et al. (2019) Identification of very early inflammatory markers in a porcine myocardial infarction model.
BMC Vet Res. 15 (1): 91. -
Yang, N. et al. (2018) Reduced antigen presentation capability and modified inflammatory/immunosuppressive cytokine expression of induced monocyte-derived dendritic cells from peripheral blood of piglets infected with porcine circovirus type 2.
Arch Virol. 163 (5): 1231-9. -
Liu, S. et al. (2019) Endothelial IL-8 induced by porcine circovirus type 2 affects dendritic cell maturation and antigen-presenting function.
Virol J. 16 (1): 154. -
Radlowski, E.C. et al. (2021) Combination-Feeding Causes Differences in Aspects of Systemic and Mucosal Immune Cell Phenotypes and Functions Compared to Exclusive Sow-Rearing or Formula-Feeding in Piglets.
Nutrients. 13(4):1097. -
Franzoni, G. et al. (2022) Analyses of the Impact of Immunosuppressive Cytokines on Porcine Macrophage Responses and Susceptibility to Infection to African Swine Fever Viruses.
Pathogens. 11 (2): 166.
Further Reading
-
Piriou-Guzylack, L. (2008) Membrane markers of the immune cells in swine: an update.
Vet Res. 39: 54.
- UniProt
- Q85ZW4
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