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SLA Class II DR antibody | 2E9/13

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Anti Pig SLA Class II DR Antibody, clone 2E9/13 thumbnail image 1 Anti Pig SLA Class II DR Antibody, clone 2E9/13 thumbnail image 2 Anti Pig SLA Class II DR Antibody, clone 2E9/13 thumbnail image 3
Anti Pig SLA Class II DR Antibody, clone 2E9/13 gallery image 1
Pig peripheral blood lymphocytes stained with Mouse anti Pig SLA Class II DR (MCA2314GA) followed by FITC conjugated Goat anti Mouse IgG antibody (STAR70)
Anti Pig SLA Class II DR Antibody, clone 2E9/13 gallery image 2
Published customer image:
Mouse anti Pig SLA Class II DR antibody, clone 2E9/13 (MCA2314GA) used for the evaluation of MHC expression by immunohistochemistry on formalin fixed, paraffin embedded tissue sections.
Image caption:
Analysis of decellularized liver materials.
(a,b) Representative H&E staining of the ultrastructure of native liver and decellularized liver matrix. Scale bars = 100 μm. DAPI staining (c,d) and Sirius Red staining (e,f) illustrate a lack of residual cellular components and the preservation of matrix components in the decellularized ECM. Scale bars = 200 μm. Immunohistochemical staining of native and decellularized liver tissues for α-Gal (g,h), SLA-2 (i,j), SLA-DRα (k,l) are shown. Scale bars = 100 μm. (m) Semi-quantitative PCR for the α-Gal, SLA-DRα, PERV, and SLA-2 antigens in the liver scaffolds. Na = native liver; De = decellularized liver matrix.

From: Wang Y, Bao J, Wu X, Wu Q, Li Y, Zhou Y, Li L, Bu H.
Genipin crosslinking reduced the immunogenicity of xenogeneic decellularized porcine whole-liver matrices through regulation of immune cell proliferation and polarization.
Sci Rep. 2016 Apr 21;6:24779.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Anti Pig SLA Class II DR Antibody, clone 2E9/13 gallery image 3
Published customer image:
Mouse anti Porcine SLA II DR antibody, clone 2E9/13 (MCA2314GA) used for the evaluation of SLA class II expression on purified B cells in vitro over time by flow cytometry
Image caption:
CD40L induces B cell activation.
CD21+ purified B cell populations were cultured in the presence or absence of CD40L (1.0 μg/ml), gated on live cells, and then analyzed for changes in the median fluorescence intensity (MFI) of SLA-DR (A) and CD80 (B). Cells were analyzed prior to culture and at 24 h intervals for a period of 7 days. One representative experiment of two performed is displayed. Data shown are mean ± SEM. Statistical differences of *p ≤0.05, ** p ≤0.01, ***p ≤0.001 are indicated.

From: Rahe MC, Murtaugh MP (2017)
Interleukin-21 Drives Proliferation and Differentiation of Porcine Memory B Cells into Antibody Secreting Cells.
PLoS ONE 12(1): e0171171.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.

Mouse anti Pig SLA Class II DR:Alexa Fluor® 647

Mouse anti Pig SLA Class II DR:FITC

Mouse anti Pig SLA Class II DR

Product Type
Monoclonal Antibody
Clone
2E9/13
Isotype
IgG2b
Product Code Applications Pack Size List Price Quantity
MCA2314A647
Datasheet
F
SDS
100 Tests/1ml loader

MCA2314F
Datasheet
F
SDS
0.1 mg loader

MCA2314GA
Datasheet
C F IP
SDS
0.1 mg loader

Mouse anti Pig SLA Class II DR antibody, clone 2E9/13 recognizes SLA DR molecules which are expressed on all B cells, antigen presenting cells and on certain subsets of resting and activated T cells. Mouse anti Pig SLA Class II DR antibody, clone 289/13 reacts with lymphocytes from all outbred and miniature pigs so far tested, suggesting that it recognizes a monomorphic determinant of porcine SLA DR.

The major histocompatibility complex (MHC) is a cluster of genes that are important in the immune response to infections. In pigs, this is referred to as the swine leukocyte antigen (SLA) region. There are 3 major MHC class II proteins encoded by the SLA which are SLA DP, SLA DQ and SLA DR.

Mouse anti pig SLA class II DR, clone 2E9/13 immunoprecipitates a heterodimer composed of two polypeptides of ~28 and ~35 kDa from NP-40 extracts of biotin surface-labeled porcine peripheral blood mononuclear cells. Mouse anti Pig SLA Class II DR antibody, clone 289/13 is reported to inhibit the mixed lymphocyte reaction and T cell stimulation induced by African swine fever virus and staphylococcal enterotoxin B (Bullido et al. 1997).

Product Details

Target Species
Pig
Species Cross-Reactivity
Target SpeciesCross Reactivity
Bovine
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG conjugated to Alexa Fluor 647 - liquid
Product Form
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Carrier Free
Yes
Immunogen
Porcine monocytes.
Approx. Protein Concentrations
IgG concentration 0.05 mg/ml
Approx. Protein Concentrations
IgG concentration 0.1 mg/ml
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the mouse X63-Ag.8.653 myeloma cell line.

Storage Information

Storage
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Storage
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Storage
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Guarantee
12 months from date of despatch
Guarantee
12 months from date of despatch
Guarantee
12 months from date of despatch

More Information

Acknowledgements
This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchased product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
Regulatory
For research purposes only

Applications of SLA Class II DR antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat 1/5
Flow Cytometry Neat
Flow Cytometry 1/25 1/200
Immunohistology - Frozen
Immunoprecipitation
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to 1x106 cells in 100ul.
Flow Cytometry
Use 10ul of the suggested working dilution to 1x106 cells in 100ul.
Flow Cytometry
Use 10ul of the suggested working dilution to 1x106 cells in 100ul.
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Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A E WB 0.5 mg loader
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA F IF 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA F WB 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA F IF WB 0.1 mg loader
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F F 0.5 mg loader
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P C E WB 0.5 mg loader
Goat anti Mouse IgG (Fc):FITC STAR120F C F 1 mg loader
Goat anti Mouse IgG (Fc):HRP STAR120P E WB 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A F 1 ml loader
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B C E P RE WB 1 mg loader
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 F 0.5 mg loader
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 F 1 ml loader
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 C E P 0.5 mg loader
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A C E WB 1 mg loader
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P E 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA F IF WB 0.1 mg loader
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B F 1 mg loader

Negative Isotype Controls Available

Description Product Code Applications Pack Size List Price Quantity
Mouse IgG2b Negative Control:Alexa Fluor® 647 MCA691A647 F 100 Tests/1ml loader
Mouse IgG2b Negative Control:FITC MCA691F F 100 Tests loader
Mouse IgG2b Negative Control MCA691 F 100 Tests loader

Application Based External Images

Flow Cytometry

Product Specific References

References for SLA Class II DR antibody

  1. Bullido, R. et al. (1997) Characterization of five monoclonal antibodies specific for swine class II major histocompatibility antigens and crossreactivity studies with leukocytes of domestic animals.
    Dev Comp Immunol. 21 (3): 311-22.
  2. Jeong, H.J. et al. (2010) Comparative measurement of cell-mediated immune responses of swine to the M and N proteins of porcine reproductive and respiratory syndrome virus.
    Clin Vaccine Immunol. 17: 503-12.
  3. Ding, Q. et al. (2011) Human PD-L1-overexpressing porcine vascular endothelial cells induce functionally suppressive human CD4+CD25hiFoxp3+ Treg cells.
    J Leukoc Biol. 90 (1): 77-86.
  4. Wang, Y. et al. (2016) Genipin crosslinking reduced the immunogenicity of xenogeneic decellularized porcine whole-liver matrices through regulation of immune cell proliferation and polarization.
    Sci Rep. 6: 24779.
  5. Park KM et al. (2013) Generation of porcine induced pluripotent stem cells and evaluation of their major histocompatibility complex protein expression in vitro.
    Vet Res Commun. 37 (4): 293-301.
  6. Iwase H et al. (2015) Initial in vivo experience of pig artery patch transplantation in baboons using mutant MHC (CIITA-DN) pigs.
    Transpl Immunol. 32 (2): 99-108.
  7. Singleton, H. et al. (2016) Establishing Porcine Monocyte-Derived Macrophage and Dendritic Cell Systems for Studying the Interaction with PRRSV-1.
    Front Microbiol. 7: 832.
  8. Zanotti, C. et al. (2015) Differential Biological Activities of Swine Interferon-α Subtypes.
    J Interferon Cytokine Res. 35 (12): 990-1002.
  9. Rayat GR et al. (2016) First update of the International Xenotransplantation Association consensus statement on conditions for undertaking clinical trials of porcine islet products in type 1 diabetes - Chapter 3: Porcine islet product manufacturing and release testing criteria.
    Xenotransplantation. 23 (1): 38-45.
  10. Mašek J et al. (2016) Multi-layered nanofibrous mucoadhesive films for buccal and sublingual administration of drug-delivery and vaccination nanoparticles - important step towards effective mucosal vaccines.
    J Control Release. Jul 25. pii: S0168-3659(16)30471-0 [Epub ahead of print]
  11. Gardner, D.S. et al. (2016) Remote effects of acute kidney injury in a porcine model.
    Am J Physiol Renal Physiol. 310 (4): F259-71.
  12. Rahe, M.C. & Murtaugh, M.P. (2017) Interleukin-21 Drives Proliferation and Differentiation of Porcine Memory B Cells into Antibody Secreting Cells.
    PLoS One. 12 (1): e0171171.
  13. López, E. et al. (2019) Identification of very early inflammatory markers in a porcine myocardial infarction model.
    BMC Vet Res. 15 (1): 91.
  14. Yang, N. et al. (2018) Reduced antigen presentation capability and modified inflammatory/immunosuppressive cytokine expression of induced monocyte-derived dendritic cells from peripheral blood of piglets infected with porcine circovirus type 2.
    Arch Virol. 163 (5): 1231-9.

Further Reading

  1. Piriou-Guzylack, L. (2008) Membrane markers of the immune cells in swine: an update.
    Vet Res. 39: 54.

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