IgM antibody | K52 1C3
Mouse anti Porcine IgM antibody, clone K52 1C3 (MCA637GA) used for the determination of secreted porcine IgM in vitro by ELISA.
Image caption:
BAFF is a maintenance factor for porcine B cells.
CD21+ purified B cells were treated with cytokines as described and cultured in technical triplicates. Culture supernatants and cells were collected and analyzed at day 7 for number of total cells via hemocytometer (A) and flow cytometry with fixable viability dye (B). Total secreted IgM (C) and IgG (D) were determined by ELISA as described in Materials and Methods. Results are representative of three independent experiments with three individual animals. Data are mean ± SEM. Statistical differences of *p <0.05, ** p <0.01, ***p <0.001 were determined using ANOVA to compare group means followed by t-tests to calculate significance.
From: Rahe MC, Murtaugh MP (2017)
Interleukin-21 Drives Proliferation and Differentiation of Porcine Memory B Cells into Antibody Secreting Cells.
PLoS ONE 12(1): e0171171.
This is from an open access article distributed under the terms of the Creative Commons Attribution License.
Mouse anti Pig IgM antibody, clone K52 1C3 used for the measurement of ova specific IgM levels in serum by ELISA.
Image caption:
OVA-specific antibody-mediated immune responses in serum from newborn piglets gavaged with OVA then i.p. immunized with OVA at four weeks of age. Piglets (n = 5/group) were gavaged and i.p. immunized as described in Figure 1. Control newborn piglets were not gavaged or immunized with OVA. We measured serum anti-OVA IgM (A), IgA (B), IgG (C), IgG1 (D) and IgG2 (E) production on day 28, day 42 and day 49 after birth. Each data point represents an individual animal and median values are indicated by horizontal lines. *p < 0.05., **p < 0.01.
From: Pasternak JA, Ng SH, Buchanan RM, Mertins S, Mutwiri GK, Gerdts V, Wilson HL.
Oral antigen exposure in newborn piglets circumvents induction of oral tolerance in response to intraperitoneal vaccination in later life.
BMC Vet Res. 2015 Dec;11(1):350
This is from an open access article distributed under the terms of the Creative Commons Attribution License.
Mouse anti Pig IgM antibody, clone K52 1C3 used for the measurement of ova specific IgM levels in lung lavages by ELISA.
Image caption:
OVA-specific antibody-mediated immune responses in lung washes from newborn piglets gavaged with OVA then i.p. immunized with OVA at four weeks of age. Piglets (n = 5/group) were gavaged and i.p. immunized as described in Figure 1. Control newborn piglets were not gavaged or immunized with OVA. Lung lavages were collected four weeks post i.p. immunization and OVA-specific serum IgM (A), IgA (B), IgG (C), IgG1 (D), and IgG2 (E) titres were measured. ELISA titres are expressed as the reciprocal of the highest dilution resulting in a reading of two standard deviations above the negative control. Each data point represents an individual animal and median values are indicated by horizontal lines. *p < 0.05, **p < 0.01.
From: Pasternak JA, Ng SH, Buchanan RM, Mertins S, Mutwiri GK, Gerdts V, Wilson HL.
Oral antigen exposure in newborn piglets circumvents induction of oral tolerance in response to intraperitoneal vaccination in later life.
BMC Vet Res. 2015 Dec;11(1):350
This is from an open access article distributed under the terms of the Creative Commons Attribution License.
Mouse anti Pig IgM antibody, clone K52 1C3 used for the detection of IgM expressing cells in pig jejenum by immunofluorescence.
Image caption:
Colonisation expands IgM and IgA-producing mucosal plasma cells. (A) IgA staining in jejunal mucosa from a GF pig and (B) a colonised pig at 21 days of age. Sections were stained with antibodies to IgA (red) and capillary endothelium (MIL11; in blue). In both (A) and (B), (i) shows the two-colour image; (ii) shows the original greyscale image for the red channel (IgA); (iii) shows the original greyscale image for the blue channel (endothelium). Scale bar represents 10 μm. (C) IgM staining in jejunal mucosa from a GF pig and (D) a colonised pig at 21 days of age. Sections were stained with antibodies to IgM (red). Scale bar represents 10 μm. (E) Area of IgM and (F) IgA staining in jejunal sections from piglets at birth (open diamonds) and GF piglets (open squares) and colonised piglets (black squares) at 5 and 21days of age (see Table 1 for numbers of piglets in each experiment). * p<0.01.
From: Inman CF, Laycock GM, Mitchard L, Harley R, Warwick J, et al. (2012)
Neonatal Colonisation Expands a Specific Intestinal Antigen-Presenting Cell Subset Prior to CD4 T-Cell Expansion, without Altering T-Cell Repertoire.
PLoS ONE 7(3): e33707
This is from an open access article distributed under the terms of the Creative Commons Attribution License.
Mouse anti Porcine IgM antibody, clone K52 1C3 (MCA637) used to evaluate IgM in porcine blood samples by ELISA.
Image caption:
Experiment 1: Isotype profile of antibodies against S. suis serotype 7 in piglets. Blood samples were collected from randomly chosen (and tagged) piglets at one and five weeks of age from 50 vaccinated and 50 non-vaccinated (including the 20 placebos) animals to evaluate: (A) IgM titers by ELISA. The vaccination protocol is shown in Figure 1. Antibody titers for individual piglets are shown with horizontal bars representing mean ± SEM. Values significantly different are shown in the graph with corresponding p value.
From: Corsaut L, Misener M, Canning P, Beauchamp G, Gottschalk M, Segura M.
Field Study on the Immunological Response and Protective Effect of a Licensed Autogenous Vaccine to Control Streptococcus suis Infections in Post-Weaned Piglets.
Vaccines (Basel). 2020 Jul 14;8(3):384.
doi: 10.3390/vaccines8030384.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Porcine IgM antibody, clone K52 1C3 (MCA637GA) used to evaluate porcine IgM by western blotting.
Image caption:
IgM Western blot analysis of cerebrospinal fluid (CSF) of experimentally infected piglets with acute meningitis and high intracerebrospinal S. suis burden reveals only intact IgM. Piglets were intranasally infected with the indicated strains and the CSF of one pig with meningitis of each infection group was analyzed for the presence of IgM cleavage products by anti-IgM Western Blot analysis under non-reducing conditions with a monoclonal anti-IgM antibody. Protein concentrations in the CSF were adjusted before loading the 6% polyacrylamide gel. As a control (lanes 7–10), CSF of the same piglets was incubated with 5 μg/ml rIdeSsuis. Asterisks indicate IgM cleavage products for the last positive lane. Marker bands (in kDa) are shown on the left-hand side.
From: Rungelrath V, Weiße C, Schütze N, Müller U, Meurer M, Rohde M, Seele J, Valentin-Weigand P, Kirschfink M, Beineke A, Schrödl W, Bergmann R, Baums CG.
IgM cleavage by Streptococcus suis. reduces IgM bound to the bacterial surface and is a novel complement evasion mechanism.
Virulence. 2018;9(1):1314-37.
doi: 10.1080/21505594.2018.1496778.
From: Insert article citation with space after colon and Hyperlink to PubMed record.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Porcine IgM antibody, clone K52 1C3 (MCA637GA) used to evaluate porcine IgM by flow cytometry and immunofluorescence.
Image caption:
S. suis strain 10 (wt) and the complemented mutant 10ΔideSsuis∇ideSsuis_EcoRI (∇ideSsuis_EcoRI) cleave porcine IgM. (b, c) The indicated S. suis strains were grown to an OD600 of 0.8, incubated in anti-S. suis serotype 2 hyperimmune serum for 0.5 hours at 4°C and then for four hours at 37°C. IgM labeling of the bacterial surface was analyzed before and after incubation at 37°C by flow cytometry (n = 6) (b) and fluorescent microscopy (c) using a monoclonal anti-IgM antibody and a phycoerythrin (PE)-labeled secondary antibody. The % reduction in IgM labeling was calculated by subtracting the percental amount of IgM positive bacteria after a four-hour incubation period at 37°C from an initially one hundred percent positive population before incubation at 37°C. (c) DAPI (4ʹ,6 diamidino-2-phenylindole) dye in blue was used to stain DNA. IgM, labeled by the monoclonal anti IgM antibody and the PE-labeled secondary antibody appears in pink. Bars and error bars indicate mean and standard deviation. Significant differences are indicated by asterisks. Probabilities were considered as follows p <0.05 *, p <0.01 **, p <0.001 ***.
From: Rungelrath V, Weiße C, Schütze N, Müller U, Meurer M, Rohde M, Seele J, Valentin-Weigand P, Kirschfink M, Beineke A, Schrödl W, Bergmann R, Baums CG.
IgM cleavage by Streptococcus suis. reduces IgM bound to the bacterial surface and is a novel complement evasion mechanism.
Virulence. 2018;9(1):1314-37.
doi: 10.1080/21505594.2018.1496778.
From: Insert article citation with space after colon and Hyperlink to PubMed record.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Filter by Application:
E IF WB F ResetMouse anti Pig IgM
- Product Type
- Monoclonal Antibody
- Clone
- K52 1C3
- Isotype
- IgG1
- Specificity
- IgM
Antibody Quality and Performance Guarantee
| Mouse anti Pig IgM antibody, clone K52 1C3 recognizes porcine IgM heavy chain. No cross-reactivity with porcine IgA and IgG is seen in ELISA. |
- Target Species
- Pig
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Carrier Free
- Yes
- Immunogen
- Porcine IgM
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells of immunised mice were fused with cells of the P3 - X63 - Ag 8.653 mouse myeloma line.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
1/5000 | 1/100,000 |
| Flow Cytometry | |
||
| Immunohistology - Frozen | |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Mouse IgG1 Negative Control | MCA928 | F | 100 Tests |
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| List Price | Your Price | ||||||
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| Description | Mouse IgG1 Negative Control | ||||||
References for IgM antibody
-
Leitão, A. et al. (2001) The non-haemadsorbing African swine fever virus isolate ASFV/NH/P68 provides a model for defining the protective anti-virus immune response.
J Gen Virol. 82 (Pt 3): 513-23. -
Baltes, N. et al. (2001) Actinobacillus pleuropneumoniae iron transport and urease activity: effects on bacterial virulence and host immune response.
Infect Immun. 69 (1): 472-8. -
Hamano, M. et al. (2007) Detection of antibodies to Japanese encephalitis virus in the wild boars in Hiroshima prefecture, Japan.
Epidemiol Infect. 135: 974-7. -
Andersen, J.K. et al. (1999) Systematic characterization of porcine ileal Peyer's patch, I. apoptosis-sensitive immature B cells are the predominant cell type.
Immunology. 98 (4): 612-21. -
Bailey, M. (2004) Effects of infection with transmissible gastroenteritis virus on concomitant immune responses to dietary and injected antigens.
Clin Diagn Lab Immunol. 11: 337-43. -
Pasternak, J.A. et al. (2015) Oral antigen exposure in newborn piglets circumvents induction of oral tolerance in response to intraperitoneal vaccination in later life.
BMC Vet Res. 11: 350. -
Seele, J. et al. (2015) The immunoglobulin M-degrading enzyme of Streptococcus suis, IdeSsuis, is a highly protective antigen against serotype 2.
Vaccine. 33 (19): 2207-12. -
Stepanova, H. et al. (2011) Association of attenuated mutants of Salmonella enterica serovar Enteritidis with porcine peripheral blood leukocytes.
FEMS Microbiol Lett. 321: 37-42.
View The Latest Product References
-
Laycock, G. et al. (2012) A defined intestinal colonization microbiota for gnotobiotic pigs.
Vet Immunol Immunopathol. 149: 216-24. -
Lewis MC et al. (2013) Dietary supplementation with Bifidobacterium lactis NCC2818 from weaning reduces local immunoglobulin production in lymphoid-associated tissues but increases systemic antibodies in healthy neonates.
Br J Nutr. 110: 1243-52. -
Chen, F. et al. (2015) Generation of B Cell-Deficient Pigs by Highly Efficient CRISPR/Cas9-Mediated Gene Targeting.
J Genet Genomics. 42 (8): 437-44. -
Rahe, M.C. & Murtaugh, M.P. (2017) Interleukin-21 Drives Proliferation and Differentiation of Porcine Memory B Cells into Antibody Secreting Cells.
PLoS One. 12 (1): e0171171. -
Buermann, A. et al. (2018) Pigs expressing the human inhibitory ligand PD-L1 (CD 274) provide a new source of xenogeneic cells and tissues with low immunogenic properties.
Xenotransplantation. 25 (5): e12387. -
Corsaut, L. et al. (2020) Field Study on the Immunological Response and Protective Effect of a Licensed Autogenous Vaccine to Control Streptococcus suis Infections in Post-Weaned Piglets.
Vaccines (Basel). 8 (3): 384. -
Rungelrath, V. et al. (2018) IgM cleavage by Streptococcus suis. reduces IgM bound to the bacterial surface and is a novel complement evasion mechanism.
Virulence. 9 (1): 1314-1337.
MCA637GA
147843
158337
163583
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