IgM antibody | K52 1C3

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Mouse anti Pig IgM

Product Type
Monoclonal Antibody
Clone
K52 1C3
Isotype
IgG1
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA637GA C E F 0.1 mg
Mouse anti Pig IgM antibody, clone K52 1C3 recognizes porcine IgM heavy chain. No cross-reactivity with porcine IgA and IgG is seen in ELISA.

Product Details

Target Species
Pig
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Carrier Free
Yes
Immunogen
Porcine IgM
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells of immunised mice were fused with cells of the P3 - X63 - Ag 8.653 mouse myeloma line.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody.
Shelf Life
18 months from date of despatch.

More Information

Regulatory
For research purposes only

Applications of IgM antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/5000 1/100,000
Flow Cytometry
Immunohistology - Frozen
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul

Negative Isotype Controls Available

Description Product Code Pack Size Applications List Price Quantity
Mouse IgG1 Negative Control MCA928 100 Tests F

Application Based External Images

ELISA

Flow Cytometry

Product Specific References

References for IgM antibody

  1. Leitão, A. et al. (2001) The non-haemadsorbing African swine fever virus isolate ASFV/NH/P68 provides a model for defining the protective anti-virus immune response.
    J Gen Virol. 82 (Pt 3): 513-23.
  2. Baltes, N. et al. (2001) Actinobacillus pleuropneumoniae iron transport and urease activity: effects on bacterial virulence and host immune response.
    Infect Immun. 69 (1): 472-8.
  3. Hamano, M. et al. (2007) Detection of antibodies to Japanese encephalitis virus in the wild boars in Hiroshima prefecture, Japan.
    Epidemiol Infect. 135: 974-7.
  4. Andersen, J.K. et al. (1999) Systematic characterization of porcine ileal Peyer's patch, I. apoptosis-sensitive immature B cells are the predominant cell type.
    Immunology. 98 (4): 612-21.
  5. Bailey, M. (2004) Effects of infection with transmissible gastroenteritis virus on concomitant immune responses to dietary and injected antigens.
    Clin Diagn Lab Immunol. 11: 337-43.
  6. Pasternak, J.A. et al. (2015) Oral antigen exposure in newborn piglets circumvents induction of oral tolerance in response to intraperitoneal vaccination in later life.
    BMC Vet Res. 11: 350.
  7. Seele, J. et al. (2015) The immunoglobulin M-degrading enzyme of Streptococcus suis, IdeSsuis, is a highly protective antigen against serotype 2.
    Vaccine. 33 (19): 2207-12.
  8. Stepanova, H. et al. (2011) Association of attenuated mutants of Salmonella enterica serovar Enteritidis with porcine peripheral blood leukocytes.
    FEMS Microbiol Lett. 321: 37-42.
  9. Laycock, G. et al. (2012) A defined intestinal colonization microbiota for gnotobiotic pigs.
    Vet Immunol Immunopathol. 149: 216-24.
  10. Lewis MC et al. (2013) Dietary supplementation with Bifidobacterium lactis NCC2818 from weaning reduces local immunoglobulin production in lymphoid-associated tissues but increases systemic antibodies in healthy neonates.
    Br J Nutr. 110: 1243-52.
  11. Chen, F. et al. (2015) Generation of B Cell-Deficient Pigs by Highly Efficient CRISPR/Cas9-Mediated Gene Targeting.
    J Genet Genomics. 42 (8): 437-44.
  12. Rahe, M.C. & Murtaugh, M.P. (2017) Interleukin-21 Drives Proliferation and Differentiation of Porcine Memory B Cells into Antibody Secreting Cells.
    PLoS One. 12 (1): e0171171.