IgG2 antibody | K68 Ig2

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Mouse anti Pig IgG2

Product Type
Monoclonal Antibody
Clone
K68 Ig2
Isotype
IgG1
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA636GA E 0.1 mg
Mouse anti Pig IgG2, clone K68 Ig2 recognizes porcine IgG2 and shows no cross-reactivity with IgG1, IgA or IgM. IgG2 (IgG2a and IgG2b), along with IgG1, IgG3 and IgG4 comprise the major known subclasses of IgG in swine. Combined, the various subclases of IgG comprise approximately 85% of immunoglobulin in porcine serum.

Studies using clone K68 Ig2 have demonstrated that along with IFNγ, porcine IgG2 levels are significantly elevated in parasite infections by worm species such as Schistosoma japonicum (Tian, F. et. al. 2010). With changes in agricultural practices and the ermergence of new strains of porcine diseases, it has been suggested that new methods of vaccine development should be investigated. Studies have shown that antibodies recognizing porcine immunoglobulins such as clone K68 Ig2 may have potential use in porcine vaccine development studies (Rodríguez-Calvo, T. et. al. 2010).

Clone K68 Ig2 forms part of a range of monoclonal antibodies specific for porcine immunoglobulins and immunoglobulin subclasses that are available from Bio-Rad.

Product Details

Target Species
Pig
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Carrier Free
Yes
Immunogen
Porcine IgG2
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised mice were fused with cells of the mouse P3-X63-Ag8.653 myeloma cell line.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch

More Information

Regulatory
For research purposes only

Applications of IgG2 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/1000 1/50,000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Human anti Mouse IgG1:HRP HCA036P 0.1 mg E
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A 0.5 mg E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed) STAR117D549GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed) STAR117D649GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA 0.1 mg F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F 0.5 mg F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P 0.5 mg E WB
Goat anti Mouse IgG (Fc):FITC STAR120F 1 mg C F
Goat anti Mouse IgG (Fc):HRP STAR120P 1 mg E WB
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A 1 ml F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B 1 mg C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 0.5 mg F
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 1 ml F
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 0.5 mg C E P
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A 1 mg C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P 1 mg E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA 0.1 mg F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B 1 mg F

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
Mouse anti Pig IgG1 MCA635GA 0.1 mg E
Mouse anti Pig IgA MCA638GA 0.1 mg C E F

Application Based External Images

ELISA

Product Specific References

References for IgG2 antibody

  1. Rivera, E. et al. (2003) Ginseng extract in aluminium hydroxide adjuvanted vaccines improves the antibody response of pigs to porcine parvovirus and Erysipelothrix rhusiopathiae.
    Vet Immunol Immunopathol. 91 (1): 19-27.
  2. Nejsum, P. et al. (2009) Population dynamics of Trichuris suis in trickle-infected pigs.
    Parasitology. 136:691-7.
  3. Tian, F. et al. (2010) Immune Events Associated with High Level Protection against Schistosoma japonicum Infection in Pigs Immunized with UV-Attenuated Cercariae.
    PLoS One. 2010 Oct 15;5(10):e13408.
  4. Bailey, M. et al. (2004) Effects of infection with transmissible gastroenteritis virus on concomitant immune responses to dietary and injected antigens
    Clin Diagn Lab Immunol. 11:337-43.
  5. Lin, D. et al. (2011) Multiple vaccinations with UV- attenuated cercariae in pig enhance protective immunity against Schistosoma japonicum infection as compared to single vaccination.
    Parasit Vectors. 4:103.
  6. Lefevre, E.A. et al. (2012) Immune responses in pigs vaccinated with adjuvanted and non-adjuvanted A(H1N1)pdm/09 influenza vaccines used in human immunization programmes.
    PLoS One. 7: e32400.
  7. Baums CG et al. (2010) Immunogenicity of an autogenous Streptococcus suis bacterin in preparturient sows and their piglets in relation to protection after weaning.
    Clin Vaccine Immunol. 17 (10): 1589-97.
  8. Jayashi, C.M. et al. (2012) Characterisation of antibody responses in pigs induced by recombinant oncosphere antigens from Taenia solium.
    Vaccine. pii: S0264-410X(12)01503-4.
  9. Rodríguez-Calvo, T. et al. (2010) New vaccine design based on defective genomes that combines features of attenuated and inactivated vaccines.
    PLoS One. 5: e10414.
  10. Schmied, J. et al. (2012) Effect of Heat-Killed Escherichia coli, Lipopolysaccharide, and Muramyl Dipeptide Treatments on the Immune Response Phenotype and Allergy in Neonatal Pigs Sensitized to the Egg White Protein Ovomucoid.
    Clin Vaccine Immunol. 19:1955-64.
  11. Weber, T.E. and Spurlock, M.E. (2004) Leptin alters antibody isotype in the pig in vivo, but does not regulate cytokine expression or stimulate STAT3 signaling in peripheral blood monocytes in vitro.
    J Anim Sci. 82:1630-40.
  12. Pasternak, J.A. et al. (2015) Oral antigen exposure in newborn piglets circumvents induction of oral tolerance in response to intraperitoneal vaccination in later life.
    BMC Vet Res. 11 (1): 350.
  13. Blanco E et al. (2016) Full protection of swine against foot-and-mouth disease by a bivalent B-cell epitope dendrimer peptide.
    Antiviral Res. Mar 5. pii: S0166-3542(16)30132-2. [Epub ahead of print]
  14. Goyette-Desjardins G et al. (2016) Protection against Streptococcus suis Serotype 2 Infection Using a Capsular Polysaccharide Glycoconjugate Vaccine.
    Infect Immun. 84 (7): 2059-75.
  15. Williams, A.R. et al. (2017) Dietary cinnamaldehyde enhances acquisition of specific antibodies following helminth infection in pigs.
    Vet Immunol Immunopathol. 189: 43-52.