IgG1 antibody | K139 3C8
Mouse anti Pig IgG1 antibody, clone K139 3C8 used for the measurement of ova specific IgG1 levels in lung lavages by ELISA.
Image caption:
OVA-specific antibody-mediated immune responses in lung washes from newborn piglets gavaged with OVA then i.p. immunized with OVA at four weeks of age. Piglets (n = 5/group) were gavaged and i.p. immunized as described in Figure 1. Control newborn piglets were not gavaged or immunized with OVA. Lung lavages were collected four weeks post i.p. immunization and OVA-specific serum IgM (A), IgA (B), IgG (C), IgG1 (D), and IgG2 (E) titres were measured. ELISA titres are expressed as the reciprocal of the highest dilution resulting in a reading of two standard deviations above the negative control. Each data point represents an individual animal and median values are indicated by horizontal lines. *p < 0.05, **p < 0.01.
From: Pasternak JA, Ng SH, Buchanan RM, Mertins S, Mutwiri GK, Gerdts V, Wilson HL. Oral antigen exposure in newborn piglets circumvents induction of oral tolerance in response to intraperitoneal vaccination in later life.
BMC Vet Res. 2015 Dec;11(1):350.
Mouse anti Pig IgG1 antibody, clone K139 3C8 used for the measurement of ova specific IgG1 levels in serum by ELISA.
Image caption:
OVA-specific antibody-mediated immune responses in serum from newborn piglets gavaged with OVA then i.p. immunized with OVA at four weeks of age. Piglets (n = 5/group) were gavaged and i.p. immunized as described in Figure 1. Control newborn piglets were not gavaged or immunized with OVA. We measured serum anti-OVA IgM (A), IgA (B), IgG (C), IgG1 (D) and IgG2 (E) production on day 28, day 42 and day 49 after birth. Each data point represents an individual animal and median values are indicated by horizontal lines. *p < 0.05., **p < 0.01.
From: Pasternak JA, Ng SH, Buchanan RM, Mertins S, Mutwiri GK, Gerdts V, Wilson HL. Oral antigen exposure in newborn piglets circumvents induction of oral tolerance in response to intraperitoneal vaccination in later life.
BMC Vet Res. 2015 Dec;11(1):350.
Mouse anti Pig IgG1, clone K139 3C8 recognizes porcine IgG1 and no cross-reaction has been observed with porcine IgA, IgG2 or IgM.
IgG1, along with IgG2 (IgG2a and IgG2b), IgG3 and IgG4 comprise the major known subclasses of IgG in swine. Combined, the various subclasses of IgG comprise approximately 85% of immunoglobulin in porcine serum.
In addition to clone K139 3C8, a range of monoclonal antibodies recognizing other porcine IgG subclasses and immunoglobulins are available.
IgG1, along with IgG2 (IgG2a and IgG2b), IgG3 and IgG4 comprise the major known subclasses of IgG in swine. Combined, the various subclasses of IgG comprise approximately 85% of immunoglobulin in porcine serum.
In addition to clone K139 3C8, a range of monoclonal antibodies recognizing other porcine IgG subclasses and immunoglobulins are available.
Product Details
- Target Species
- Pig
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Carrier Free
- Yes
- Immunogen
- Porcine IgG1.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised mice were fused with cells of the mouse P3-X63-Ag8.653 myeloma cell line.
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch
More Information
- Regulatory
- For research purposes only
Applications of IgG1 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | 1/50 | 1/5000 | |
| Immunohistology - Frozen | |||
| Immunohistology - Paraffin | |||
| Immunohistology - Resin |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Copyright © 2020 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
Useful Reagents Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Mouse anti Pig IgG2 | MCA636GA | E | 0.1 mg |
|
|
| Mouse anti Pig IgA | MCA638GA | C E F | 0.1 mg |
|
Product Specific References
References for IgG1 antibody
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Rivera, E. et al. (2003) Ginseng extract in aluminium hydroxide adjuvanted vaccines improves the antibody response of pigs to porcine parvovirus and Erysipelothrix rhusiopathiae.
Vet. Immunol. Immunopathol. 91; 19 - 27. -
Nejsum, P. et al. (2009) Population dynamics of Trichuris suis in trickle-infected pigs.
Parasitology. 136: 691-7. -
Tian, F. et al. (2010) Immune Events Associated with High Level Protection against Schistosoma japonicum Infection in Pigs Immunized with UV-Attenuated Cercariae.
PLoS One. 5(10): e13408. -
Bailey, M. et al. (2004) Effects of infection with transmissible gastroenteritis virus on concomitant immune responses to dietary and injected antigens.
Clin Diagn Lab Immunol. 11: 337-43. -
Lin, D. et al. (2011) Multiple vaccinations with UV- attenuated cercariae in pig enhance protective immunity against Schistosoma japonicum infection as compared to single vaccination.
Parasit Vectors. 4:103. -
Lefevre, EA. et al. (2012) Immune responses in pigs vaccinated with adjuvanted and non-adjuvanted A(H1N1)pdm/09 influenza vaccines used in human immunization programmes.
PLoS One. 7: e32400. -
Baums, C.G. et al. (2010) Immunogenicity of an autogenous Streptococcus suis bacterin in preparturient sows and their piglets in relation to protection after weaning.
Clin Vaccine Immunol. 17: 1589-97. -
Jayashi, C.M. et al. (2012) Characterisation of antibody responses in pigs induced by recombinant oncosphere antigens from Taenia solium.
Vaccine. pii: S0264-410X(12)01503-4. -
Rodríguez-Calvo, T. et al. (2010) New vaccine design based on defective genomes that combines features of attenuated and inactivated vaccines.
PLoS One. 5: e10414. -
Schmied, J. et al. (2012) Effect of Heat-Killed Escherichia coli, Lipopolysaccharide, and Muramyl Dipeptide Treatments on the Immune Response Phenotype and Allergy in Neonatal Pigs Sensitized to the Egg White Protein Ovomucoid.
Clin Vaccine Immunol. 19: 1955-64. -
Weber, T.E. and Spurlock, M.E. (2004) Leptin alters antibody isotype in the pig in vivo, but does not regulate cytokine expression or stimulate STAT3 signaling in peripheral blood monocytes in vitro.
J Anim Sci. 82: 1630-40. -
Pasternak JA et al. (2015) Oral antigen exposure in newborn piglets circumvents induction of oral tolerance in response to intraperitoneal vaccination in later life.
BMC Vet Res. 11 (1): 350. -
Blanco, E. et al. (2016) Full protection of swine against foot-and-mouth disease by a bivalent B-cell epitope dendrimer peptide.
Antiviral Res. Mar 5. pii: S0166-3542(16)30132-2. [Epub ahead of print] -
Williams, A.R. et al. (2017) Dietary cinnamaldehyde enhances acquisition of specific antibodies following helminth infection in pigs.
Vet Immunol Immunopathol. 189: 43-52. -
Williams, A.R. et al. (2017) A polyphenol-enriched diet and Ascaris suum infection modulate mucosal immune responses and gut microbiota composition in pigs.
PLoS One. 12 (10): e0186546.
