CD8 Alpha antibody | MIL12
Mouse anti Pig wCD8 alpha antibody, clone MIL12 (MCA1223GA) used for the evaluation of CD7α expression on porcine lymphocytes by flow cytometry.
Image caption:
Frequency of leukocyte subsets in PBMC and LPMC using FCM analysis A) shows a representative FCM analysis (n=4) from PBMCs (left) and LPMC (right) isolated from 6 week old pigs were analysed on the frequencies of different immune cell subsets using three-color FCM. Forward scatter (FSC) and side scatter (SSC) were used to gate on leukocytes (PBMCs (a) and LPMC (h) for further analysis of myeloid cells (CD172+; b and i), NK cells (CD3- CD8α+; c and j), B cells (CD21+; e and l) and T cells (CD3+; d and k). Gated T cells were then further analysed on their CD4/CD8α expression to determine the frequency of CTLs (CD4-CD8αhigh; f and m) and CD4+ T cells (f and m). CD8α expression of CD4+ T cells was also investigated to define the frequency of experienced CD4+ T cells (CD8α+CD4+; g and n). (o) Summarizes the results of this FCM analysis of all four analysed animals. Percentages from the PBMC population are represented by a closed circle and the percentages from the LPMC populations are represented by an open box. (p) and (q) is a pie chart showing the relative average percentage of PBMCs and LPMCs from all 4 animals.
From: Pasternak JA, Ng S, Kaser T, Meurens F, Wilson HL (2014) Grouping Pig-Specific Responses to Mitogen with Similar Responder Animals may Facilitate the Interpretation of Results Obtained in an Out-Bred Animal Model.
J Vaccines Vaccin 5:242.
Mouse anti Pig wCD8 alpha antibody, clone MIL12 recognizes an epitope on the alpha chain of porcine wCD8. Clone MIl12 was clustered at the Third International Swine CD Workshop (Haverson et al. 2001). Clone MIL12 was determined to bind to the CD8a epitope on the alpha chain based on its staining pattern on T lymphocytes and on its ability to block binding of the previously characterized CD8a antibody clone 76-2-11 to T lymphocytes (Saalmuller et al.2001).
Product Details
- Target Species
- Pig
- Product Form
- Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
- Reconstitution
- Reconstitute with 1.0 ml distilled water
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin - Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin - Carrier Free
- Yes
- Immunogen
- Porcine mesenteric lymphocytes.
- Approx. Protein Concentrations
- IgG concentration 0.1mg/ml
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised BALB/c mice were fused with cells of the P3 - X63 - Ag.653 myeloma cell line.
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Prior to reconstitution store at +4oC. Following reconstitution store at +4oC.
DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 18 months from date of despatch.
- Guarantee
- 18 months from date of despatch.
- Guarantee
- 12 months from date of reconstitution.
More Information
- Regulatory
- For research purposes only
Applications of CD8 Alpha antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry | Neat | ||
| Flow Cytometry | 1/25 | 1/200 | |
| Immunohistology - Frozen | |||
| Flow Cytometry | Neat |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Copyright © 2019 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
Negative Isotype Controls Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Mouse IgG2a Negative Control:FITC | MCA929F | 100 Tests | F | ||
| Mouse IgG2a Negative Control | MCA929 | 100 Tests | F | ||
| Mouse IgG2a Negative Control:RPE | MCA929PE | 100 Tests | F |
Product Specific References
References for CD8 Alpha antibody
-
Sarradell, J. et al. (2003) A morphologic and immunohistochemical study of the bronchus-associated lymphoid tissue of pigs naturally infected with Mycoplasma hyopneumoniae.
Vet Pathol. 40: 395-404. -
Kick, A.R. et al. (2011) Evaluation of peripheral lymphocytes after weaning and vaccination for Mycoplasma hyopneumoniae.
Res Vet Sci. 91 (3): e68-72. -
Tambuyzer, B.R. et al. (2012) Osteopontin alters the functional profile of porcine microglia in vitro.
Cell Biol Int. 36 (12): 1233-8. -
Cao, D. et al. (2010) Synthetic innate defence regulator peptide enhances in vivo immunostimulatory effects of CpG-ODN in newborn piglets.
Vaccine. 28: 6006-13. -
Clapperton, M. et al. (2005) Innate immune traits differ between Meishan and Large White pigs.
Vet Immunol Immunopathol. 104: 131-44. -
Goujon, J.M. et al. (2000) Influence of cold-storage conditions on renal function of autotransplanted large pig kidneys.
Kidney Int. 58: 838-50. -
Piva, A. et al. (2005) Activated carbon does not prevent the toxicity of culture material containing fumonisin B1 when fed to weanling piglets.
J Anim Sci. 83 (8): 1939-47. -
Hauet, T. et al. (2002) Polyethylene glycol reduces the inflammatory injury due to cold ischemia/reperfusion in autotransplanted pig kidneys.
Kidney Int. 62: 654-67. -
Kick, A.R. et al. (2012) Effects of stress associated with weaning on the adaptive immune system in pigs.
J Anim Sci. 90: 649-56. -
Shi, K. et al. (2008) Changes in peripheral blood leukocyte subpopulations in piglets co-infected experimentally with porcine reproductive and respiratory syndrome virus and porcine circovirus type 2.
Vet Microbiol. 129: 367-77. -
Spreeuwenberg, M.A. et al. (2001) Small intestine epithelial barrier function is compromised in pigs with low feed intake at weaning.
J Nutr. 131: 1520-7. -
Clapperton, M. et al. (2008) Pig peripheral blood mononuclear leucocyte subsets are heritable and genetically correlated with performance.
Animal. 2: 1575-84. -
Leifer, I. et al. (2012) Characterization of C-strain "Riems" TAV-epitope escape variants obtained through selective antibody pressure in cell culture.
Vet Res. 43: 33. -
Tuchscherer, M. et al. (2012) Effects of inadequate maternal dietary protein:carbohydrate ratios during pregnancy on offspring immunity in pigs.
BMC Vet Res. 8: 232. -
Lu, X. et al. (2012) Genome-wide association study for T lymphocyte subpopulations in swine.
BMC Genomics. 13: 488. -
Swamy, H.V. et al. (2003) Effects of feeding a blend of grains naturally contaminated with Fusarium mycotoxins on growth and immunological measurements of starter pigs, and the efficacy of a polymeric glucomannan mycotoxin adsorbent.
J Anim Sci. 81: 2792-803. -
Monroy-Salazar, H.G. et al. (2012) Effects of a live yeast dietary supplement on fecal coliform counts and on peripheral blood CD4+ and CD8+ lymphocyte subpopulations in nursery pigs.
J Swine Health Prod. 20: 276–282. -
Ostrowska, E. et al. (2004) Effects of dietary conjugated linoleic acid on haematological and humoral responses in the grower pig.
Australian Journal of Agricultural Research 55: 711–718 -
Carter, D.B. et al. (2002) Phenotyping of transgenic cloned piglets.
Cloning Stem Cells. 4: 131-45. -
Stenfeldt, C. et al. (2014) Morphologic and phenotypic characteristics of myocarditis in two pigs infected by foot-and mouth disease virus strains of serotypes O or A.
Acta Vet Scand. 56: 42. -
Zeigler, B.M. et al. (2015) The development and validation of methods for evaluating the immune system in preweaning piglets.
Food Chem Toxicol. 84: 197-207. -
Pasternak, J. A. (2014) Grouping Pig-Specific Responses to Mitogen with Similar Responder Animals may Facilitate the Interpretation of Results Obtained in an Out-Bred Animal Model
Journal of Vaccines & Vaccination. 05 (05). -
Marinaro M et al. (2015) Changes in peripheral blood leucocytes of sheep experimentally infected with Mycoplasma agalactiae.
Vet Microbiol. 175 (2-4): 257-64. -
Liermann, W. et al. (2016) Effects of two commercial diets and technical feed treatment on stomach lesions and immune system of fattening pigs.
J Anim Physiol Anim Nutr (Berl). Nov 2. [Epub ahead of print] -
Hemmink, J.D. et al. (2016) Distinct immune responses and virus shedding in pigs following aerosol, intra-nasal and contact infection with pandemic swine influenza A virus, A(H1N1)09.
Vet Res. 47 (1): 103. -
López, E. et al. (2019) Identification of very early inflammatory markers in a porcine myocardial infarction model.
BMC Vet Res. 15 (1): 91. -
Hu, Z. et al. (2019) Genomic variant in porcine TNFRSF1A gene and its effects on TNF signaling pathway in vitro.
Gene. 700: 105-9.
Further Reading
-
Piriou-Guzylack, L. (2008) Membrane markers of the immune cells in swine: an update.
Vet Res. 39: 54.
