CD8 Alpha antibody | MIL12
Mouse anti Pig wCD8 alpha antibody, clone MIL12 (MCA1223GA) used for the evaluation of CD7α expression on porcine lymphocytes by flow cytometry.
Image caption:
Frequency of leukocyte subsets in PBMC and LPMC using FCM analysis A) shows a representative FCM analysis (n=4) from PBMCs (left) and LPMC (right) isolated from 6 week old pigs were analysed on the frequencies of different immune cell subsets using three-color FCM. Forward scatter (FSC) and side scatter (SSC) were used to gate on leukocytes (PBMCs (a) and LPMC (h) for further analysis of myeloid cells (CD172+; b and i), NK cells (CD3- CD8α+; c and j), B cells (CD21+; e and l) and T cells (CD3+; d and k). Gated T cells were then further analysed on their CD4/CD8α expression to determine the frequency of CTLs (CD4-CD8αhigh; f and m) and CD4+ T cells (f and m). CD8α expression of CD4+ T cells was also investigated to define the frequency of experienced CD4+ T cells (CD8α+CD4+; g and n). (o) Summarizes the results of this FCM analysis of all four analysed animals. Percentages from the PBMC population are represented by a closed circle and the percentages from the LPMC populations are represented by an open box. (p) and (q) is a pie chart showing the relative average percentage of PBMCs and LPMCs from all 4 animals.
From: Pasternak JA, Ng S, Kaser T, Meurens F, Wilson HL (2014) Grouping Pig-Specific Responses to Mitogen with Similar Responder Animals may Facilitate the Interpretation of Results Obtained in an Out-Bred Animal Model.
J Vaccines Vaccin 5:242.
R-PE conjugated Mouse anti CD8 alpha antibody, clone MIL12 (MCA1223PE) used to identify CD8+ lymphocytes by flow cytometry.
Image caption:
Gating strategy: (a) determination of the position of lymphocytes (red) on the basic dot plot (FSC-A versus SSC-A); (b) removing doublets and agglomerates from the analysis; (c) analysis of the representation of CD3+ and CD21+ lymphocytes; (d) analysis of the representation of CD4+ and CD8+ lymphocytes; (e) determination of the representation of CD4+CD25+ lymphocytes on histogram from CD4+ positive cells (gated with green).
From: Bujňák, L.; Hreško Šamudovská, A.; Mudroňová, D.; Naď, P.; Marcinčák, S.; Maskaľová, I.; Harčárová, M.; Karaffová, V.; Bartkovský, M.
The Effect of Dietary Humic Substances on Cellular Immunity and Blood Characteristics in Piglets.
Agriculture 2023, 13, 636.
doi: 10.3390/agriculture13030636
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
R-PE conjugated Mouse anti CD8 alpha antibody, clone MIL12 (MCA1223PE) used to identify CD8+ lymphocytes by flow cytometry.
Image caption:
Effect of humic substances on the percentage of lymphocytes: (a) CD3+; (b) CD21+; (c) CD4+CD8-; (d) CD4-CD8+; (e) CD4+CD8+; (f) CD4+CD25+; and (g) ratio of CD4:CD8 lymphocytes in the blood of the piglets. Column labelled with asterisk is significantly different from the control (*** p < 0.001).
From: Bujňák, L.; Hreško Šamudovská, A.; Mudroňová, D.; Naď, P.; Marcinčák, S.; Maskaľová, I.; Harčárová, M.; Karaffová, V.; Bartkovský, M.
The Effect of Dietary Humic Substances on Cellular Immunity and Blood Characteristics in Piglets.
Agriculture 2023, 13, 636.
doi: 10.3390/agriculture13030636
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Pig wCD8 Alpha
- Product Type
- Monoclonal Antibody
- Clone
- MIL12
- Isotype
- IgG2a
- Specificity
- CD8 Alpha
Antibody Quality and Performance Guarantee
| Mouse anti Pig wCD8 alpha antibody, clone MIL12 recognizes an epitope on the alpha chain of porcine wCD8. Clone MIl12 was clustered at the Third International Swine CD Workshop (Haverson et al. 2001). Mouse anti Pig wCD8 alpha antibody, clone MIL12 was determined to bind to the CD8a epitope on the alpha chain based on its staining pattern on T lymphocytes and on its ability to block binding of the previously characterized CD8a antibody clone 76-2-11 to T lymphocytes (Saalmuller et al.2001). |
- Target Species
- Pig
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% sodium azide (NaN3)
- Carrier Free
- Yes
- Immunogen
- Porcine mesenteric lymphocytes.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunized BALB/c mice were fused with cells of the P3 - X63 - Ag.653 myeloma cell line.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
1/25 | 1/200 |
| Immunohistology - Frozen | |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10μl of the suggested working dilution to label 106 cells in 100μl
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Mouse IgG2a Negative Control | MCA929 | F | 100 Tests |
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| List Price | Your Price | ||||||
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| Description | Mouse IgG2a Negative Control | ||||||
References for CD8 Alpha antibody
-
Goujon, J.M. et al. (2000) Influence of cold-storage conditions on renal function of autotransplanted large pig kidneys.
Kidney Int. 58: 838-50. -
Spreeuwenberg, M.A. et al. (2001) Small intestine epithelial barrier function is compromised in pigs with low feed intake at weaning.
J Nutr. 131: 1520-7. -
Carter, D.B. et al. (2002) Phenotyping of transgenic cloned piglets.
Cloning Stem Cells. 4: 131-45. -
Hauet, T. et al. (2002) Polyethylene glycol reduces the inflammatory injury due to cold ischemia/reperfusion in autotransplanted pig kidneys.
Kidney Int. 62: 654-67. -
Swamy, H.V. et al. (2003) Effects of feeding a blend of grains naturally contaminated with Fusarium mycotoxins on growth and immunological measurements of starter pigs, and the efficacy of a polymeric glucomannan mycotoxin adsorbent.
J Anim Sci. 81: 2792-803. -
Sarradell, J. et al. (2003) A morphologic and immunohistochemical study of the bronchus-associated lymphoid tissue of pigs naturally infected with Mycoplasma hyopneumoniae.
Vet Pathol. 40: 395-404. -
Ostrowska, E. et al. (2004) Effects of dietary conjugated linoleic acid on haematological and humoral responses in the grower pig
Austral J Agric Res. 55 (7): 711. -
Piva, A. et al. (2005) Activated carbon does not prevent the toxicity of culture material containing fumonisin B1 when fed to weanling piglets.
J Anim Sci. 83 (8): 1939-47.
View The Latest Product References
-
Clapperton, M. et al. (2005) Innate immune traits differ between Meishan and Large White pigs.
Vet Immunol Immunopathol. 104: 131-44. -
Shi, K. et al. (2008) Changes in peripheral blood leukocyte subpopulations in piglets co-infected experimentally with porcine reproductive and respiratory syndrome virus and porcine circovirus type 2.
Vet Microbiol. 129: 367-77. -
Clapperton, M. et al. (2008) Pig peripheral blood mononuclear leucocyte subsets are heritable and genetically correlated with performance.
Animal. 2: 1575-84. -
Kick, A.R. et al. (2011) Evaluation of peripheral lymphocytes after weaning and vaccination for Mycoplasma hyopneumoniae.
Res Vet Sci. 91 (3): e68-72. -
Monroy-Salazar, H.G. et al. (2012) Effects of a live yeast dietary supplement on fecal coliform counts and on peripheral blood CD4+ and CD8+ lymphocyte subpopulations in nursery pigs.
J Swine Health Prod. 20: 276-82. -
Lu, X. et al. (2012) Genome-wide association study for T lymphocyte subpopulations in swine.
BMC Genomics. 13: 488. -
Leifer, I. et al. (2012) Characterization of C-strain "Riems" TAV-epitope escape variants obtained through selective antibody pressure in cell culture.
Vet Res. 43: 33. -
Tuchscherer, M. et al. (2012) Effects of inadequate maternal dietary protein:carbohydrate ratios during pregnancy on offspring immunity in pigs.
BMC Vet Res. 8: 232. -
Kick, A.R. et al. (2012) Effects of stress associated with weaning on the adaptive immune system in pigs.
J Anim Sci. 90: 649-56. -
Tambuyzer, B.R. et al. (2012) Osteopontin alters the functional profile of porcine microglia in vitro.
Cell Biol Int. 36 (12): 1233-8. -
Stenfeldt, C. et al. (2014) Morphologic and phenotypic characteristics of myocarditis in two pigs infected by foot-and mouth disease virus strains of serotypes O or A.
Acta Vet Scand. 56: 42. -
Alex, P.J. (2014) Grouping Pig-Specific Responses to Mitogen with Similar Responder Animals may Facilitate the Interpretation of Results Obtained in an Out-Bred Animal Model
J Vaccines & Vaccination. 05 (05) -
Zeigler, B.M. et al. (2015) The development and validation of methods for evaluating the immune system in preweaning piglets.
Food Chem Toxicol. 84: 197-207. -
Liermann, W. et al. (2017) Effects of two commercial diets and technical feed treatment on stomach lesions and immune system of fattening pigs.
J Anim Physiol Anim Nutr (Berl). 101 (5): e414-e426. -
Hemmink, J.D. et al. (2016) Distinct immune responses and virus shedding in pigs following aerosol, intra-nasal and contact infection with pandemic swine influenza A virus, A(H1N1)09.
Vet Res. 47 (1): 103. -
López, E. et al. (2019) Identification of very early inflammatory markers in a porcine myocardial infarction model.
BMC Vet Res. 15 (1): 91. -
Hu, Z. et al. (2019) Genomic variant in porcine TNFRSF1A gene and its effects on TNF signaling pathway in vitro.
Gene. 700: 105-9. -
Fogle, J.E. et al. (2019) Antibiotic Therapy Does Not Alter the Humoral Response to Vaccination for Porcine Circovirus 2 in Weaned Pigs.
Vet Sci. 6 (2): 51. -
Nielsen, O.L. et al. (2022) A porcine model of subcutaneous Staphylococcus aureus infection: a pilot study.
APMIS. 130 (7): 359-70. -
Maciag, S.S. et al. (2022) On the influence of the source of porcine colostrum in the development of early immune ontogeny in piglets.
Sci Rep. 12 (1): 15630. -
Wu, M.C. et al. (2023) A protein-based subunit vaccine with biological adjuvants provides effective protection against Pasteurella multocida in pigs.
Vet Res. 54 (1): 17. -
Bujňák, L. et al. (2023) The Effect of Dietary Humic Substances on Cellular Immunity and Blood Characteristics in Piglets
Agriculture. 13 (3): 636. -
Haach, V. et al. (2023) A polyvalent virosomal influenza vaccine induces broad cellular and humoral immunity in pigs.
Virol J. 20 (1): 181. -
Maciag, S. et al. (2022) Effects of freezing storage on the stability of maternal cellular and humoral immune components in porcine colostrum.
Vet Immunol Immunopathol. 254: 110520. -
Forner, R. et al. (2021) Distribution difference of colostrum-derived B and T cells subsets in gilts and sows.
PLoS One. 16 (5): e0249366. -
Novotný, J. et al. (2024) The Use of Flow Cytometry in the Analysis of Sows’ Colostrum and Milk
Folia Veterinaria. 68 (3): 44-51.
Further Reading
-
Piriou-Guzylack, L. (2008) Membrane markers of the immune cells in swine: an update.
Vet Res. 39: 54.
- Synonyms
- CD8
MCA1223GA
159634
1609
165182
165624
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