CD31 antibody | LCI-4

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MCA1746GA
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C F IF IP 0.1 mg loader
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Mouse anti Pig CD31, clone LCI-4 recognizes porcine CD31, also known as Platelet endothelial cell adhesion molecule (PECAM-1). CD31 is constitutively expressed by platelets, monocytes and some lymphocytes, it is expressed by endothelial cells at a level, an order of magnitude greater that of other cell types (Fawcwett et al.1995). The extracellular region contains six Ig-like domains. Mouse anti Pig CD31, clone LCI-4 is cross reactive with human CD31 and binds to the 5th extracellular Ig domain, proximal to the transmembrane region as demonstrated by human CD31 domain deletion mutants (Nasu et al.1999).

Mouse anti Pig CD31, clone LCI-4 immunoprecipitates a protein of ~130 kDa from lysates of porcine aortic endothelial cells and is strongly expressed at cell junctions (Nasu et al. 1999).

Target Species
Pig
Species Cross-Reactivity
Target SpeciesCross Reactivity
Human
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% sodium azide (NaN3)
Carrier Free
Yes
Immunogen
Porcine CD31/human IgGFc fusion protein.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/10 1/100
Immunofluorescence
Immunohistology - Frozen
Immunoprecipitation
Western Blotting
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10μl of the suggested working dilution to label 106 cells or 100μl whole blood

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References for CD31 antibody

  1. Nasu, K. et al. (1999) Alpha-galactosyl-mediated activation of porcine endothelial cells: studies on CD31 and VE-cadherin in adhesion and signaling.
    Transplantation. 68: 861-7.
  2. Evans, P.C. et al. (2001) Signaling through CD31 protects endothelial cells from apoptosis.
    Transplantation. 71 (3): 343-4.
  3. Gesslein, B. et al. (2010) Mitogen-activated protein kinases in the porcine retinal arteries and neuroretina following retinal ischemia-reperfusion.
    Mol Vis. 16: 392-407.
  4. Gyöngyösi, M. et al. (2010) Differential effect of ischaemic preconditioning on mobilisation and recruitment of haematopoietic and mesenchymal stem cells in porcine myocardial ischaemia-reperfusion.
    Thromb Haemost. 104 (2): 376-84.
  5. Iohara, K. et al. (2008) A novel stem cell source for vasculogenesis in ischemia: subfraction of side population cells from dental pulp.
    Stem Cells. 26 (9): 2408-18.
  6. Campos, E. et al. (2004) In vitro effect of classical swine fever virus on a porcine aortic endothelial cell line
    Vet Res. 35: 625-33.
  7. Takeda, S. et al. (2006) Differential origin for endothelial and mesangial cells after transplantation of pig fetal renal primordia into rats.
    Transpl Immunol. 15: 211-5.
  8. Katchman, H. et al. (2008) Embryonic porcine liver as a source for transplantation: advantage of intact liver implants over isolated hepatoblasts in overcoming homeostatic inhibition by the quiescent host liver.
    Stem Cells. 26: 1347-55.
  9. View The Latest Product References
  10. Poirier, N. et al. (2010) Inducing CTLA-4-dependent immune regulation by selective CD28 blockade promotes regulatory T cells in organ transplantation.
    Sci Transl Med. 2 (17): 17ra10.
  11. Tchorsh-Yutsis, D. et al. (2009) Pig embryonic pancreatic tissue as a source for transplantation in diabetes: transient treatment with anti-LFA1, anti-CD48, and FTY720 enables long-term graft maintenance in mice with only mild ongoing immunosuppression.
    Diabetes. 58: 1585-94.
  12. Waksman, R. et al. (2006) Intracoronary photodynamic therapy reduces neointimal growth without suppressing re-endothelialisation in a porcine model.
    Heart. 92: 1138-44.
  13. Chatelais, M. et al. (2011) Gene transfer of the adaptor Lnk (SH2B3) prevents porcine endothelial cell activation and apoptosis: implication for xenograft's cytoprotection.
    Xenotransplantation. 18: 108-20.
  14. Peng, X. et al. (2015) Phenotypic and Functional Properties of Porcine Dedifferentiated Fat Cells during the Long-Term Culture In Vitro.
    Biomed Res Int. 2015: 673651.
  15. Chitalia, V.C. et al. (2011) Matrix-embedded endothelial cells are protected from the uremic milieu.
    Nephrol Dial Transplant. 26: 3858-65.
  16. Kang, S.D. et al. (2013) Isolation of functional human endothelial cells from small volumes of umbilical cord blood.
    Ann Biomed Eng. 41 (10): 2181-92.
  17. Graham, J.J. et al. (2010) Long-term tracking of bone marrow progenitor cells following intracoronary injection post-myocardial infarction in swine using MRI.
    Am J Physiol Heart Circ Physiol. 299: H125-33.
  18. Azimzadeh, A.M. et al. (2014) Development of a consensus protocol to quantify primate anti-non-Gal xenoreactive antibodies using pig aortic endothelial cells.
    Xenotransplantation. 21 (6): 555-66.
  19. Andrée, B. et al. (2014) Successful re-endothelialization of a perfusable biological vascularized matrix (BioVaM) for the generation of 3D artificial cardiac tissue.
    Basic Res Cardiol. 109: 441.
  20. Sokoli, .A. et al. (2013) Mycoplasma suis infection results endothelial cell damage and activation: new insight into the cell tropism and pathogenicity of hemotrophic mycoplasma.
    Vet Res.44: 6.
  21. Ramirez, H.A. et al. (2015) Comparative Genomic, MicroRNA, and Tissue Analyses Reveal Subtle Differences between Non-Diabetic and Diabetic Foot Skin.
    PLoS One. 10 (8): e0137133.
  22. Balaoing, L.R. et al. (2015) Laminin Peptide-Immobilized Hydrogels Modulate Valve Endothelial Cell Hemostatic Regulation.
    PLoS One. 10 (6): e0130749.
  23. Leitão, A.F. et al. (2016) A Novel Small-Caliber Bacterial Cellulose Vascular Prosthesis: Production, Characterization, and Preliminary In Vivo Testing.
    Macromol Biosci. 16 (1): 139-50.
  24. Barsotti, M.C. et al. (2015) Oligonucleotide biofunctionalization enhances endothelial progenitor cell adhesion on cobalt/chromium stents.
    J Biomed Mater Res A. 103 (10): 3284-92.
  25. Zhang, Q. et al. (2015) Engineering vascularized soft tissue flaps in an animal model using human adipose-derived stem cells and VEGF+PLGA/PEG microspheres on a collagen-chitosan scaffold with a flow-through vascular pedicle.
    Biomaterials. 73: 198-213.
  26. Puperi, D.S. et al. (2015) 3-Dimensional spatially organized PEG-based hydrogels for an aortic valve co-culture model.
    Biomaterials. 67: 354-64.
  27. Chen, P. et al. (2017) Altered expression of eNOS, prostacyclin synthase, prostaglandin G/H synthase, and thromboxane synthase in porcine aortic endothelial cells after exposure to human serum-relevance to xenotransplantation.
    Cell Biol Int. 41 (7): 798-808.
  28. Rayat, G.R. et al. (2016) First update of the International Xenotransplantation Association consensus statement on conditions for undertaking clinical trials of porcine islet products in type 1 diabetes - Chapter 3: Porcine islet product manufacturing and release testing criteria.
    Xenotransplantation. 23 (1): 38-45.
  29. Maïga, S. et al. (2017) Renal auto-transplantation promotes cortical microvascular network remodeling in a preclinical porcine model.
    PLoS One. 12 (7): e0181067.
  30. Strbo, N. et al. (2019) Single cell analyses reveal specific distribution of anti-bacterial molecule Perforin-2 in human skin and its modulation by wounding and Staphylococcus aureus infection.
    Exp Dermatol. 28 (3): 225-32.
  31. Ramm, R. et al. (2016) Decellularized GGTA1-KO pig heart valves do not bind preformed human xenoantibodies.
    Basic Res Cardiol. 111 (4): 39.
  32. Hätinen, O.A. et al. (2019) Isolation of fresh endothelial cells from porcine heart for cardiovascular studies: a new fast protocol suitable for genomic, transcriptomic and cell biology studies.
    BMC Mol Cell Biol. 20 (1): 32.
  33. Bernardini, C. et al. (2020) Effects of Hydrogen Sulfide Donor NaHS on Porcine Vascular Wall-Mesenchymal Stem Cells.
    Int J Mol Sci. 21(15):5267.
  34. Jaff, N. et al. (2018) Transcriptomic analysis of the harvested endothelial cells in a swine model of mechanical thrombectomy.
    Neuroradiology. 60 (7): 759-68.
  35. Zhu, H. et al. (2022) Production of cultured meat from pig muscle stem cells.
    Biomaterials. 287: 121650.

Further Reading

  1. Piriou-Guzylack, L. (2008) Membrane markers of the immune cells in swine: an update.
    Vet Res. 39: 54.

Immunofluorescence

Synonyms
PECAM-1
UniProt
Q95242
Entrez Gene
PECAM1
GO Terms
GO:0007155 cell adhesion
GO:0016021 integral to membrane
GO:0030054 cell junction
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