CD31 antibody | LCI-4
Mouse anti Pig CD31 antibody, clone LCI-4 used for the detection of vascular endothelium in pigs by immunofluorescence.
Image caption:
VECs produce, release, and actively cleave von Willebrand factor. (A) Non-stimulated VECs seeded on hydrogels (3.4 kDa) functionalized with RKR (left) or RGDS (right) stained for CD31 (red) and VWF (green). Nucleus was stained with DAPI (blue). Scale bar = 50 μm. (B) Quantification of rapidly released VWF and (C) the fraction of cleaved VWF-140 fragments from histamine-stimulated VECs cultured on different molecular weight hydrogels functionalized with RKR or RGDS, or TCPS. Groups not connected by same symbols are significantly different. p < 0.05.
From: Balaoing LR, Post AD, Lin AY, Tseng H, Moake JL, Grande-Allen KJ (2015) Laminin Peptide-Immobilized Hydrogels Modulate Valve Endothelial Cell Hemostatic Regulation.
PLoS ONE 10(6): e0130749.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Mouse anti Pig CD31 antibody, clone LCI-4 used for the evaluation of PECAM-1 expression on M. suis infected porcine endothelial cells.
Image caption:
CAM expression in M. suis infected endothelial cells. The expression profiles of adhesion molecules on endothelial cells were analyzed using a FACSCanto II cell sorting system. A. Dashed lines represent the isotype control; solid lines represent cells incubated with the negative control preparation; grey histograms represent PEDSV.15 cells incubated with M. suis (1 × 104 cells/mL). B. Percent increase in expression ICAM-1 (CD54), PECAM-1 (CD31), E (CD62E)- and P (CD62P)-selectin in ECs infected with M. suis.
From: Sokoli A, Groebel K, Hoelzle K, Amselgruber WM, Mateos JM, Schneider MK, Ziegler U, Felder KM, Hoelzle LE.
Mycoplasma suis infection results endothelial cell damage and activation: new insight into the cell tropism and pathogenicity of hemotrophic mycoplasma.
Vet Res. 2013 Feb 11;44:6.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Mouse anti Pig CD31 antibody, clone LCI-4 (MCA1746GA) used for the detection of blood vessels in normal and diabetic human patient skin by immunohistochemistry of formalin fixed, paraffin embedded tissue sections.
Image caption:
Similar numbers of lymphocytes, blood vessels and proliferating keratinocytes are found in DFS and NFS. Immunostaining and quantification for CD45, a lymphocyte marker, (A, D), CD31, an endothelial cell marker, (B, E) and Ki67, a proliferation marker (C,F), reveal no differences in the number of lymphocytes, blood vessels and proliferating keratinocytes found in DFS and NFS. Ki67 immunoperoxidase staining for proliferating cells (C) shows that proliferative keratinocytes were located in the basal layer of the skin, as expected. No significant differences were found between DFS and NFS in numbers of CD45+ cells (D), number of CD31+ (E) or Ki67 positive keratinocytes (F). Bar graphs indicate mean and SD.
From: Ramirez HA, Liang L, Pastar I, Rosa AM, Stojadinovic O, Zwick TG, et al. (2015) Comparative Genomic, MicroRNA, and Tissue Analyses Reveal Subtle Differences between Non-Diabetic and Diabetic Foot Skin.
PLoS ONE 10(8): e0137133.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Mouse anti Pig CD31 antibody, clone LCI-4 used for the detection of CD31 expressing cells in porcine retina by immunofluorescence.
Image caption:
Phosphorylated p38 immunofluorescence in the retinal arteries and neuroretina. A: A representative example of phosphorylated p38 (p-p38; green) staining of the retinal arteries is shown. Double staining with the smooth muscle cell marker smooth muscle actin (SMA; red) and the endothelium cell marker CD31 (red) shows that p-p38 is primarily located in the smooth muscle layer. Similar results were seen for sham-operated and ischemia-reperfusion eyes. B: p-p38 (green) was also occasionally detected in the inner nuclear layer of the neuroretina. Double staining with protein kinase Cα (PKCα; red), a bipolar cell marker, showed that p38 was localized to bipolar cell bodies, whereas the p38 protein appears to be associated with the nucleus. The abbreviations used in the figure are outer nuclear layer (ONL), outer plexiform layer (OPL), and inner plexiform layer (IPL).
From: Gesslein B, Håkansson G, Carpio R, Gustafsson L, Perez MT, Malmsjö M.
Mitogen-activated protein kinases in the porcine retinal arteries and neuroretina following retinal ischemia-reperfusion.
Mol Vis. 2010 Mar 10;16:392-407.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Mouse anti Pig CD31 antibody, clone LCI-4 used for the evaluation of CD31 expression on porcine differentiated fat cells by flow cytometry.
Image caption:
Expression of cell surface antigens on porcine DFAT cells. Expressions of CD44, CD29, CD90, CD31, and CD34 were detected by FITC or CY3 conjugated antibody of P4 DFAT cells.
From: Peng X, Song T, Hu X, Zhou Y, Wei H, Peng J, Jiang S. (2015)
Phenotypic and Functional Properties of Porcine Dedifferentiated Fat Cells during the Long-Term Culture In Vitro.
Biomed Res Int. 2015: 673651.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Mouse anti Porcine CD31 antibody, clone LCI-4 used for the demonstration of renal vasculature by immunofluorescence on cryostat sections.
Image caption:
Renal transplantation induces vascular remodeling and interstitial fibrosis.
(D) CD31 and aminopeptidase P-positive blood microvessels from renal cortex samples from uni-nephrectomized and grafted kidneys three months after surgery (Magnification x40).
From: Maïga S, Allain G, Hauet T, Roumy J, Baulier E, Scepi M, et al. (2017)
Renal auto-transplantation promotes cortical microvascular network remodeling in a preclinical porcine model.
PLoS ONE 12(7): e0181067.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Mouse anti Porcine CD31 antibody, clone LCI-4 (MCA1746GA) used to identify porcine endothelial cells by immunofluorescence and flow cytometry.
Image caption:
Identification of functionality and purity of endothelial cells. a) Isolated cells were identified as endothelial cells by CD31 (PECAM-1) antibody (MCA1746GA, AbD Serotec) immunostaining (passage 3). b) One of the characteristics of endothelial cells is tube formation. Functional analysis of the cells was performed by using an angiogenesis assay (Matrigel GFR Membrane Matrix, #356231, Corning) (passage 2). c) Purity of the cells was ensured by using fluorescence assisted cell sorting (FACS) resulting in purity of 97.81%, which can be considered as a pure preparation of ECs (passage 2).
From: Hätinen OA, Lähteenvuo JE, Korpela HJ, Pajula JJ, Ylä-Herttuala S.
Isolation of fresh endothelial cells from porcine heart for cardiovascular studies: a new fast protocol suitable for genomic, transcriptomic and cell biology studies.
BMC Mol Cell Biol. 2019 Aug 13;20(1):32.
doi: 10.1186/s12860-019-0215-2.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Mouse anti Porcine CD31 antibody, clone LCI-4 (MCA1746GA) used to identify an endothelial cell phenotype in sheroid cutlures by immunofluorescence.
Image caption:
Effect of NaHS on the pVW–MSC gene expression profile and angiogenic attitude:
Immunofluorescence analysis of pVW–MSC’s spheroids with endothelial markers Factor VIII and CD31(both green) in the control (CTR) and NaHS treatments (10 μM), and the nuclei were stained with propidium iodide (PI) (red) scale bar = 500 μm.
From: Bernardini C, La Mantia D, Nesci S, Salaroli R, Algieri C, Pagliarani A, Zannoni A, Forni M.
Effects of Hydrogen Sulfide Donor NaHS on Porcine Vascular Wall-Mesenchymal Stem Cells.
Int J Mol Sci. 2020 Jul 24;21(15):5267.
doi: 10.3390/ijms21155267.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Porcine CD31 antibody, clone LCI-4 (MCA1746GA) used to demonstrate CD31 staining of endothelial cell membranes by immunofluorescence.
Image caption:
Immunohistochemistry staining of endothelial cells from cell culture. Endothelial cells revealed by positive CD31 membrane staining (a) and positive CD31 membrane staining and vWF cytosol staining (d). Scale bars = 100 μm
From: Jaff N, Grankvist R, Muhl L, Chireh A, Sandell M, Jonsson S, Arnberg F, Eriksson U, Holmin S.
Transcriptomic analysis of the harvested endothelial cells in a swine model of mechanical thrombectomy.
Neuroradiology. 2018 Jul;60(7):759-68.
doi: 10.1007/s00234-018-2033-1.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Pig CD31
- Product Type
- Monoclonal Antibody
- Clone
- LCI-4
- Isotype
- IgG1
- Specificity
- CD31
Antibody Quality and Performance Guarantee
| Mouse anti Pig CD31, clone LCI-4 recognizes porcine CD31, also known as Platelet endothelial cell adhesion molecule (PECAM-1). CD31 is constitutively expressed by platelets, monocytes and some lymphocytes, it is expressed by endothelial cells at a level, an order of magnitude greater that of other cell types (Fawcwett et al.1995). The extracellular region contains six Ig-like domains. Mouse anti Pig CD31, clone LCI-4 is cross reactive with human CD31 and binds to the 5th extracellular Ig domain, proximal to the transmembrane region as demonstrated by human CD31 domain deletion mutants (Nasu et al.1999). Mouse anti Pig CD31, clone LCI-4 immunoprecipitates a protein of ~130 kDa from lysates of porcine aortic endothelial cells and is strongly expressed at cell junctions (Nasu et al. 1999). |
- Target Species
- Pig
- Species Cross-Reactivity
-
Target Species Cross Reactivity Human Mouse - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% sodium azide (NaN3)
- Carrier Free
- Yes
- Immunogen
- Porcine CD31/human IgGFc fusion protein.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
1/10 | 1/100 |
| Immunofluorescence | |
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| Immunohistology - Frozen | |
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| Immunoprecipitation | |
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| Western Blotting |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10μl of the suggested working dilution to label 106 cells or 100μl whole blood
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Mouse IgG1 Negative Control | MCA928 | F | 100 Tests |
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Log in | ||
| List Price | Your Price | ||||||
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| Description | Mouse IgG1 Negative Control | ||||||
References for CD31 antibody
-
Nasu, K. et al. (1999) Alpha-galactosyl-mediated activation of porcine endothelial cells: studies on CD31 and VE-cadherin in adhesion and signaling.
Transplantation. 68: 861-7. -
Evans, P.C. et al. (2001) Signaling through CD31 protects endothelial cells from apoptosis.
Transplantation. 71 (3): 343-4. -
Campos, E. et al. (2004) In vitro effect of classical swine fever virus on a porcine aortic endothelial cell line
Vet Res. 35: 625-33. -
Waksman, R. et al. (2006) Intracoronary photodynamic therapy reduces neointimal growth without suppressing re-endothelialisation in a porcine model.
Heart. 92: 1138-44. -
Iohara, K. et al. (2008) A novel stem cell source for vasculogenesis in ischemia: subfraction of side population cells from dental pulp.
Stem Cells. 26 (9): 2408-18. -
Katchman, H. et al. (2008) Embryonic porcine liver as a source for transplantation: advantage of intact liver implants over isolated hepatoblasts in overcoming homeostatic inhibition by the quiescent host liver.
Stem Cells. 26: 1347-55. -
Tchorsh-Yutsis, D. et al. (2009) Pig embryonic pancreatic tissue as a source for transplantation in diabetes: transient treatment with anti-LFA1, anti-CD48, and FTY720 enables long-term graft maintenance in mice with only mild ongoing immunosuppression.
Diabetes. 58: 1585-94. -
Gesslein, B. et al. (2010) Mitogen-activated protein kinases in the porcine retinal arteries and neuroretina following retinal ischemia-reperfusion.
Mol Vis. 16: 392-407.
View The Latest Product References
-
Gyöngyösi, M. et al. (2010) Differential effect of ischaemic preconditioning on mobilisation and recruitment of haematopoietic and mesenchymal stem cells in porcine myocardial ischaemia-reperfusion.
Thromb Haemost. 104 (2): 376-84. -
Poirier, N. et al. (2010) Inducing CTLA-4-dependent immune regulation by selective CD28 blockade promotes regulatory T cells in organ transplantation.
Sci Transl Med. 2 (17): 17ra10. -
Graham, J.J. et al. (2010) Long-term tracking of bone marrow progenitor cells following intracoronary injection post-myocardial infarction in swine using MRI.
Am J Physiol Heart Circ Physiol. 299: H125-33. -
Chitalia, V.C. et al. (2011) Matrix-embedded endothelial cells are protected from the uremic milieu.
Nephrol Dial Transplant. 26: 3858-65. -
Sokoli, .A. et al. (2013) Mycoplasma suis infection results endothelial cell damage and activation: new insight into the cell tropism and pathogenicity of hemotrophic mycoplasma.
Vet Res.44: 6. -
Kang, S.D. et al. (2013) Isolation of functional human endothelial cells from small volumes of umbilical cord blood.
Ann Biomed Eng. 41 (10): 2181-92. -
Azimzadeh, A.M. et al. (2014) Development of a consensus protocol to quantify primate anti-non-Gal xenoreactive antibodies using pig aortic endothelial cells.
Xenotransplantation. 21 (6): 555-66. -
Peng, X. et al. (2015) Phenotypic and Functional Properties of Porcine Dedifferentiated Fat Cells during the Long-Term Culture In Vitro.
Biomed Res Int. 2015: 673651. -
Ramirez, H.A. et al. (2015) Comparative Genomic, MicroRNA, and Tissue Analyses Reveal Subtle Differences between Non-Diabetic and Diabetic Foot Skin.
PLoS One. 10 (8): e0137133. -
Balaoing, L.R. et al. (2015) Laminin Peptide-Immobilized Hydrogels Modulate Valve Endothelial Cell Hemostatic Regulation.
PLoS One. 10 (6): e0130749. -
Barsotti, M.C. et al. (2015) Oligonucleotide biofunctionalization enhances endothelial progenitor cell adhesion on cobalt/chromium stents.
J Biomed Mater Res A. 103 (10): 3284-92. -
Zhang, Q. et al. (2015) Engineering vascularized soft tissue flaps in an animal model using human adipose-derived stem cells and VEGF+PLGA/PEG microspheres on a collagen-chitosan scaffold with a flow-through vascular pedicle.
Biomaterials. 73: 198-213. -
Puperi, D.S. et al. (2015) 3-Dimensional spatially organized PEG-based hydrogels for an aortic valve co-culture model.
Biomaterials. 67: 354-64. -
Ramm, R. et al. (2016) Decellularized GGTA1-KO pig heart valves do not bind preformed human xenoantibodies.
Basic Res Cardiol. 111 (4): 39. -
Leitão, A.F. et al. (2016) A Novel Small-Caliber Bacterial Cellulose Vascular Prosthesis: Production, Characterization, and Preliminary In Vivo Testing.
Macromol Biosci. 16 (1): 139-50. -
Chen, P. et al. (2017) Altered expression of eNOS, prostacyclin synthase, prostaglandin G/H synthase, and thromboxane synthase in porcine aortic endothelial cells after exposure to human serum-relevance to xenotransplantation.
Cell Biol Int. 41 (7): 798-808. -
Maïga, S. et al. (2017) Renal auto-transplantation promotes cortical microvascular network remodeling in a preclinical porcine model.
PLoS One. 12 (7): e0181067. -
Jaff, N. et al. (2018) Transcriptomic analysis of the harvested endothelial cells in a swine model of mechanical thrombectomy.
Neuroradiology. 60 (7): 759-68. -
Strbo, N. et al. (2019) Single cell analyses reveal specific distribution of anti-bacterial molecule Perforin-2 in human skin and its modulation by wounding and Staphylococcus aureus infection.
Exp Dermatol. 28 (3): 225-32. -
Hätinen, O.A. et al. (2019) Isolation of fresh endothelial cells from porcine heart for cardiovascular studies: a new fast protocol suitable for genomic, transcriptomic and cell biology studies.
BMC Mol Cell Biol. 20 (1): 32. -
Bernardini, C. et al. (2020) Effects of Hydrogen Sulfide Donor NaHS on Porcine Vascular Wall-Mesenchymal Stem Cells.
Int J Mol Sci. 21(15):5267. -
Zhu, H. et al. (2022) Production of cultured meat from pig muscle stem cells.
Biomaterials. 287: 121650. -
Arenal, Á. et al. (2022) Effects of Cardiac Stem Cell on Postinfarction Arrhythmogenic Substrate.
Int J Mol Sci. 23 (24): 16211. -
Burdorf, L. et al. (2023) Expression of human thrombomodulin by GalTKO.hCD46 pigs modulates coagulation cascade activation by endothelial cells and during ex vivo lung perfusion with human blood.
Xenotransplantation. : e12828. -
Bernardini, C. et al. (2023) Isolation of Vascular Wall Mesenchymal Stem Cells from the Thoracic Aorta of Adult Göttingen Minipigs: A New Protocol for the Simultaneous Endothelial Cell Collection.
Animals (Basel). 13 (16): 2601.
Further Reading
-
Piriou-Guzylack, L. (2008) Membrane markers of the immune cells in swine: an update.
Vet Res. 39: 54. -
Rayat, G.R. et al. (2016) First update of the International Xenotransplantation Association consensus statement on conditions for undertaking clinical trials of porcine islet products in type 1 diabetes - Chapter 3: Porcine islet product manufacturing and release testing criteria.
Xenotransplantation. 23 (1): 38-45.
- Synonyms
- PECAM-1
- UniProt
- Q95242
- Entrez Gene
- PECAM1
- GO Terms
- GO:0007155 cell adhesion
- GO:0016021 integral to membrane
- GO:0030054 cell junction
MCA1746GA
148206
155527
163126
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