CD107a antibody | 4E9/11
Mouse anti Pig CD107a antibody, clone 4E9/11 (MCA2315GA) used for the detection of CD107a in porcine NK cells by flow cytometry
Image caption:
Analysis of degranulation capacity in NKp46-defined NK-cell subsets in blood. The cytolytic capacity of NKp46-defined NK-cell subsets (CD8α+NKp46-: blue, CD8α+NKp46+: green) isolated from blood was analysed after receptor triggering. Cells were stimulated with rhIL-2 and rpIL-15 overnight. Triggering of NK-receptors was performed by using monoclonal antibodies against NKp46, CD16 or a combination of both. Irrelevant isotype-matched antibody served as negative control. NK-cell receptor mediated degranulation was assessed by measuring the expression of CD107a on the cell surface by four-colour flow cytometry after one hour incubation. CD107a expression was measured on CD3- lymphocytes (not shown), followed by gating on the respective NKp46-defined NK subsets. (A) Numbers indicate the percentage of CD107a+ cells and the mean fluorescence intensity of CD107a within respective NKp46-gates. Results are representative of experiments with five different animals. (B) CD107a expression analyses of five animals analysed. The proportion of CD107a+ cells within the different NKp46-defined subsets is shown in the upper graphs. Percentage of CD107a+ NK cells was calculated by subtracting spontaneous degranulation observed in cultures stimulated with isotype-control antibodies from the frequency of CD107a+ cells in cultures stimulated with NKp46 and/or CD16 mAbs. The lower graphs show the mean fluorescence intensity of CD107a within the respective subsets. Mean values are represented by a black bar. Significant differences between the subsets are indicated (* = p <0.05, ** = p<0.01).
From: Mair KH, Müllebner A, Essler SE, Duvigneau JC, Storset AK, Saalmüller A, Gerner W.
Porcine CD8αdim/-NKp46high NK cells are in a highly activated state.
Vet Res. 2013 Mar 1;44:13.
This is from an open access article distributed under the terms of a Creative Commons Attribution License.
Mouse anti Pig CD107a antibody, clone 4E9/11 (MCA2315GA) used for the detection of CD107a in porcine NK cells by flow cytometry
Image caption:
NKp46high NK cells in spleen showed the highest cytolytic capacity. The cytolytic capacity of NKp46-defined NK-cell subsets (CD8α+NKp46-: blue, CD8α+NKp46+: green, CD8αdim/-NKp46high: red) isolated from spleen was analysed after receptor-mediated degranulation. Cells were stimulated and gated for flow cytometric analysis as outlined in Figure 4. (A) Numbers indicate the percentage of CD107a+ cells and the mean fluorescence intensity of CD107a within respective NKp46-gates. Results are representative of experiments with five different animals. (B) CD107a expression analyses of five animals analysed. The proportion of CD107a+ cells within the different NKp46-defined subsets is shown in the upper graphs. Percentage of CD107a+ NK cells was calculated by subtracting spontaneous degranulation observed in cultures stimulated with isotype-control antibodies from the frequency of CD107a+ cells in cultures stimulated with NKp46 and/or CD16 mAbs. The lower graphs show the mean fluorescence intensity of CD107a within the respective subsets. Mean values are represented by a black bar. Significant differences between the subsets are indicated (* = p < 0.05, ** = p <0.01).
From: Mair KH, Müllebner A, Essler SE, Duvigneau JC, Storset AK, Saalmüller A, Gerner W.
Porcine CD8αdim/-NKp46high NK cells are in a highly activated state.
Vet Res. 2013 Mar 1;44:13.
This is from an open access article distributed under the terms of a Creative Commons Attribution License.
Alexafluor 647 conjugated Mouse anti Porcine CD107a antibody, clone 4E9/11 (MCA2315A647) used for the evaluation of LAMP-1 expression on porcine CD8β+ T cells by flow cytometry.
Image caption:
Proliferation, degranulation and cytokine production by FLUAVsw-specific CD8β+ T cells. Violet-labeled PBMCs isolated at 2, 6 and 9 weeks post primary infection were restimulated twice with FLUAVsw (infection strain, MOI = 0.1; day 0 and day 5 of culture) and analyzed by FCM on day 6 of culture. (A) CD8β+ T cells were gated (not shown) and analyzed for violet proliferation, surface CD107a expression and intracellular accumulation of IFN-γ and TNF-α. Contour plots show all possible marker combinations. Exemplary raw data of one non-infected control animal (#3) and one infected animal (#8) is shown for week 6 post primary infection. (B) Boolean gating was applied in order to identify multifunctional CD8β+ T cells, and resulting CD8β+ T-cell subsets were grouped by number of functions. Displayed is the frequency of CD8β+ T cells carrying out two or more functions within total CD8β+ T cells. Data of six FLUAVsw-infected animals (#4-9) and two non-infected control animals (#1, #3) is shown.
From: Talker SC, Koinig HC, Stadler M, Graage R, Klingler E, Ladinig A, Mair KH, Hammer SE, Weissenböck H, Dürrwald R, Ritzmann M, Saalmüller A, Gerner W.
Magnitude and kinetics of multifunctional CD4+ and CD8β+ T cells in pigs infected with swine influenza A virus.
Vet Res. 2015 May 14;46:52.
This image is from an open access article distributed under the terms of a Creative Commons Attribution License.
Mouse anti Pig CD107a
- Product Type
- Monoclonal Antibody
- Clone
- 4E9/11
- Isotype
- IgG1
- Specificity
- CD107a
Antibody Quality and Performance Guarantee
| Mouse anti Pig CD107a, clone 4E9/11 recognizes porcine CD107a, a cell surface antigen, also known as lysosomal-associated membrane protein-1 or LAMP-1. CD107a is a type 1 single pass transmembrane glycoprotein expressed on macrophages and more weakly on monocytes and granulocytes. |
- Target Species
- Pig
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% sodium azide (NaN3)
- Carrier Free
- Yes
- Immunogen
- Porcine alveolar macrophages.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunized BALB/c mice were fused with cells of the mouse X63-Ag.8.653 myeloma cell line.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry 1 |  |
1/25 | 1/50 |
| Immunohistology - Frozen | |
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| Immunohistology - Paraffin 2 | |
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| Immunoprecipitation | |
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| Western Blotting | |
- 1 Membrane permeabilization is required for this application. The use of Leucoperm (Product Code BUF09) is recommended for this purpose.
- 2 This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.
Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10μl of the suggested working dilution to label 1x106 cells in 100μl
- Histology Positive Control Tissue
- Porcine spleen
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Mouse IgG1 Negative Control | MCA928 | F | 100 Tests |
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| List Price | Your Price | ||||||
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| Description | Mouse IgG1 Negative Control | ||||||
References for CD107a antibody
-
Bullido, R. et al. (1997) Monoclonal antibodies specific for porcine monocytes/macrophages: macrophage heterogeneity in the pig evidenced by the expression of surface antigens.
Tissue Antigens. 49 (4): 403-13. -
Carrillo, A. et al. (2002) Isolation and characterization of immortalized porcine aortic endothelial cell lines.
Vet Immunol Immunopathol. 89 (1-2): 91-8. -
Domenech, N. et al. (2003) Identification of porcine macrophages with monoclonal antibodies in formalin-fixed, paraffin-embedded tissues.
Vet Immunol Immunopathol. 94 (1-2): 77-81. -
Sánchez-Torres, C. et al. (2003) Expression of porcine CD163 on monocytes/macrophages correlates with permissiveness to African swine fever infection.
Arch Virol. 148 (12): 2307-23. -
Toka, F.N. et al. (2009) Natural killer cell dysfunction during acute infection with foot-and-mouth disease virus.
Clin Vaccine Immunol. 16: 1738-49. -
Toka, F.N. et al. (2009) Activation of porcine natural killer cells and lysis of foot-and-mouth disease virus infected cells.
J Interferon Cytokine Res. 29 (3): 179-92. -
Mair, K.H. et al. (2013) Porcine CD8αdim/-NKp46high NK cells are in a highly activated state.
Vet Res. 44: 13. -
Cruz, J.L. et al. (2013) Alphacoronavirus Protein 7 Modulates Host Innate Immune Response
J Virol. 87: 9754-67.
View The Latest Product References
-
Bullers, S.J. et al. (2014) The human tissue-biomaterial interface: a role for PPARγ-dependent glucocorticoid receptor activation in regulating the CD163+ M2 macrophage phenotype.
Tissue Eng Part A. 20: 2390-401. -
van Hout, G.P. et al. (2015) Invasive surgery reduces infarct size and preserves cardiac function in a porcine model of myocardial infarction.
J Cell Mol Med. 19 (11): 2655-63. -
Talker, S.C. et al. (2015) Magnitude and kinetics of multifunctional CD4+ and CD8β+ T cells in pigs infected with swine influenza A virus.
Vet Res. 46: 52. -
Bongoni, A.K. et al. (2015) Porcine extrahepatic vascular endothelial asialoglycoprotein receptor 1 mediates xenogeneic platelet phagocytosis in vitro and in human-to-pig ex vivo xenoperfusion.
Transplantation. 99 (4): 693-701. -
Dash, R. et al. (2018) Dose-Dependent Cardioprotection of Moderate (32°C) Versus Mild (35°C) Therapeutic Hypothermia in Porcine Acute Myocardial Infarction.
JACC Cardiovasc Interv. 11 (2): 195-205. -
Nishimura, Y. et al. (2023) Application of polyglycolic acid sheets and basic fibroblast growth factor to prevent esophageal stricture after endoscopic submucosal dissection in pigs.
J Gastroenterol. 58 (11): 1094-104. -
Álvarez, B. et al. (2023) Porcine Macrophage Markers and Populations: An Update.
Cells. 12 (16): 2103.
Further Reading
-
Piriou-Guzylack, L. (2008) Membrane markers of the immune cells in swine: an update.
Vet Res. 39: 54.
- Synonyms
- LAMP-1
MCA2315GA
149602
1610
163391
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