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CD107a antibody | 4E9/11

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Anti Pig CD107a Antibody, clone 4E9/11 thumbnail image 1 Anti Pig CD107a Antibody, clone 4E9/11 thumbnail image 2 Anti Pig CD107a Antibody, clone 4E9/11 thumbnail image 3 Anti Pig CD107a Antibody, clone 4E9/11 thumbnail image 4
Anti Pig CD107a Antibody, clone 4E9/11 gallery image 1
Pig peripheral blood granulocytes stained with Mouse anti Pig CD107a (MCA2315GA) followed by FITC conjugated Goat anti Mouse IgG antibody (STAR117F). Membrane permeabilisation was achieved with Leucoperm ™ (BUF09A)
Anti Pig CD107a Antibody, clone 4E9/11 gallery image 2
Published customer image:
Mouse anti Pig CD107a antibody, clone 4E9/11 (MCA2315GA) used for the detection of CD107a in porcine NK cells by flow cytometry
Image caption:
Analysis of degranulation capacity in NKp46-defined NK-cell subsets in blood. The cytolytic capacity of NKp46-defined NK-cell subsets (CD8α+NKp46-: blue, CD8α+NKp46+: green) isolated from blood was analysed after receptor triggering. Cells were stimulated with rhIL-2 and rpIL-15 overnight. Triggering of NK-receptors was performed by using monoclonal antibodies against NKp46, CD16 or a combination of both. Irrelevant isotype-matched antibody served as negative control. NK-cell receptor mediated degranulation was assessed by measuring the expression of CD107a on the cell surface by four-colour flow cytometry after one hour incubation. CD107a expression was measured on CD3- lymphocytes (not shown), followed by gating on the respective NKp46-defined NK subsets. (A) Numbers indicate the percentage of CD107a+ cells and the mean fluorescence intensity of CD107a within respective NKp46-gates. Results are representative of experiments with five different animals. (B) CD107a expression analyses of five animals analysed. The proportion of CD107a+ cells within the different NKp46-defined subsets is shown in the upper graphs. Percentage of CD107a+ NK cells was calculated by subtracting spontaneous degranulation observed in cultures stimulated with isotype-control antibodies from the frequency of CD107a+ cells in cultures stimulated with NKp46 and/or CD16 mAbs. The lower graphs show the mean fluorescence intensity of CD107a within the respective subsets. Mean values are represented by a black bar. Significant differences between the subsets are indicated (* = p <0.05, ** = p<0.01).

From: Mair KH, Müllebner A, Essler SE, Duvigneau JC, Storset AK, Saalmüller A, Gerner W.
Porcine CD8αdim/-NKp46high NK cells are in a highly activated state.
Vet Res. 2013 Mar 1;44:13.
This is from an open access article distributed under the terms of a Creative Commons Attribution License.
Anti Pig CD107a Antibody, clone 4E9/11 gallery image 3
Published customer image:
Mouse anti Pig CD107a antibody, clone 4E9/11 (MCA2315GA) used for the detection of CD107a in porcine NK cells by flow cytometry
Image caption:
NKp46high NK cells in spleen showed the highest cytolytic capacity. The cytolytic capacity of NKp46-defined NK-cell subsets (CD8α+NKp46-: blue, CD8α+NKp46+: green, CD8αdim/-NKp46high: red) isolated from spleen was analysed after receptor-mediated degranulation. Cells were stimulated and gated for flow cytometric analysis as outlined in Figure 4. (A) Numbers indicate the percentage of CD107a+ cells and the mean fluorescence intensity of CD107a within respective NKp46-gates. Results are representative of experiments with five different animals. (B) CD107a expression analyses of five animals analysed. The proportion of CD107a+ cells within the different NKp46-defined subsets is shown in the upper graphs. Percentage of CD107a+ NK cells was calculated by subtracting spontaneous degranulation observed in cultures stimulated with isotype-control antibodies from the frequency of CD107a+ cells in cultures stimulated with NKp46 and/or CD16 mAbs. The lower graphs show the mean fluorescence intensity of CD107a within the respective subsets. Mean values are represented by a black bar. Significant differences between the subsets are indicated (* = p < 0.05, ** = p <0.01).

From: Mair KH, Müllebner A, Essler SE, Duvigneau JC, Storset AK, Saalmüller A, Gerner W.
Porcine CD8αdim/-NKp46high NK cells are in a highly activated state.
Vet Res. 2013 Mar 1;44:13.
This is from an open access article distributed under the terms of a Creative Commons Attribution License.
Anti Pig CD107a Antibody, clone 4E9/11 gallery image 4
Published customer image:
Alexafluor 647 conjugated Mouse anti Porcine CD107a antibody, clone 4E9/11 (MCA2315A647) used for the evaluation of LAMP-1 expression on porcine CD8β+ T cells by flow cytometry.
Image caption:
Proliferation, degranulation and cytokine production by FLUAVsw-specific CD8β+ T cells. Violet-labeled PBMCs isolated at 2, 6 and 9 weeks post primary infection were restimulated twice with FLUAVsw (infection strain, MOI = 0.1; day 0 and day 5 of culture) and analyzed by FCM on day 6 of culture. (A) CD8β+ T cells were gated (not shown) and analyzed for violet proliferation, surface CD107a expression and intracellular accumulation of IFN-γ and TNF-α. Contour plots show all possible marker combinations. Exemplary raw data of one non-infected control animal (#3) and one infected animal (#8) is shown for week 6 post primary infection. (B) Boolean gating was applied in order to identify multifunctional CD8β+ T cells, and resulting CD8β+ T-cell subsets were grouped by number of functions. Displayed is the frequency of CD8β+ T cells carrying out two or more functions within total CD8β+ T cells. Data of six FLUAVsw-infected animals (#4-9) and two non-infected control animals (#1, #3) is shown.

From: Talker SC, Koinig HC, Stadler M, Graage R, Klingler E, Ladinig A, Mair KH, Hammer SE, Weissenböck H, Dürrwald R, Ritzmann M, Saalmüller A, Gerner W.
Magnitude and kinetics of multifunctional CD4+ and CD8β+ T cells in pigs infected with swine influenza A virus.
Vet Res. 2015 May 14;46:52.
This image is from an open access article distributed under the terms of a Creative Commons Attribution License.

Mouse anti Pig CD107a:Alexa Fluor® 647

Mouse anti Pig CD107a:FITC

Mouse anti Pig CD107a

Product Type
Monoclonal Antibody
Clone
4E9/11
Isotype
IgG1
Product Code Applications Pack Size List Price Quantity
MCA2315A647
Datasheet
F*
SDS
100 Tests/1ml loader

MCA2315F
Datasheet
F*
SDS
0.1 mg loader

MCA2315GA
Datasheet
C F* IP P* WB
SDS
0.1 mg loader

Mouse anti Pig CD107a, clone 4E9/11 recognizes porcine CD107a, a cell surface antigen, also known as lysosomal-associated membrane protein-1 or LAMP-1.

CD107a is a type 1 single pass transmembrane glycoprotein expressed on macrophages and more weakly on monocytes and granulocytes.

Product Details

Target Species
Pig
Product Form
Purified IgG conjugated to Alexa Fluor® 647- liquid
Product Form
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Carrier Free
Yes
Immunogen
Porcine alveolar macrophages.
Approx. Protein Concentrations
IgG concentration 0.05mg/ml
Approx. Protein Concentrations
IgG concentration 0.1 mg/ml
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the mouse X63-Ag.8.653 myeloma cell line.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.

Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.

Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch
Guarantee
12 months from date of despatch
Guarantee
12 months from date of despatch

More Information

Acknowledgements
This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
Regulatory
For research purposes only

Applications of CD107a antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1 Neat
Flow Cytometry 1 Neat
Flow Cytometry 1 1/25 1/50
Immunohistology - Frozen
Immunohistology - Paraffin 2
Immunoprecipitation
Western Blotting
  1. 1 Membrane permeabilisation is required for this application. Bio-Rad recommends the use of Leucoperm™ (Product Code BUF09) for this purpose.
  1. 1 Membrane permeabilisation is required for this application. Bio-Rad recommends the use of Leucoperm™ (Product Code BUF09) for this purpose.
  1. 1 Membrane permeabilisation is required for this application. Bio-Rad recommends the use of Leucoperm™ (Product Code BUF09) for this purpose.
  2. 2 This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.

    Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
Histology Positive Control Tissue
Porcine spleen.
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A E WB 0.5 mg loader
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA F IF 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA F WB 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA F IF WB 0.1 mg loader
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F F 0.5 mg loader
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P C E WB 0.5 mg loader
Goat anti Mouse IgG (Fc):FITC STAR120F C F 1 mg loader
Goat anti Mouse IgG (Fc):HRP STAR120P E WB 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A F 1 ml loader
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B C E P RE WB 1 mg loader
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 F 0.5 mg loader
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 F 1 ml loader
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 C E P 0.5 mg loader
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A C E WB 1 mg loader
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P E 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA F IF WB 0.1 mg loader
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B F 1 mg loader

Negative Isotype Controls Available

Description Product Code Applications Pack Size List Price Quantity
Mouse IgG1 Negative Control:Alexa Fluor® 647 MCA928A647 F 100 Tests/1ml loader
Mouse IgG1 Negative Control:FITC MCA928F F 100 Tests loader
Mouse IgG1 Negative Control MCA928 F 100 Tests loader

Application Based External Images

Flow Cytometry

Immunofluorescence

Immunohistology - Paraffin

Product Specific References

References for CD107a antibody

  1. Bullido, R. et al. (1997) Monoclonal antibodies specific for porcine monocytes/macrophages: macrophage heterogeneity in the pig evidenced by the expression of surface antigens.
    Tissue Antigens. 49 (4): 403-13.
  2. Carrillo, A. et al. (2002) Isolation and characterization of immortalized porcine aortic endothelial cell lines.
    Vet Immunol Immunopathol. 89 (1-2): 91-8.
  3. Domenech, N. et al. (2003) Identification of porcine macrophages with monoclonal antibodies in formalin-fixed, paraffin-embedded tissues.
    Vet Immunol Immunopathol. 94 (1-2): 77-81.
  4. Sánchez-Torres, C. et al. (2003) Expression of porcine CD163 on monocytes/macrophages correlates with permissiveness to African swine fever infection.
    Arch Virol. 148 (12): 2307-23.
  5. Toka, F.N. et al. (2009) Natural killer cell dysfunction during acute infection with foot-and-mouth disease virus.
    Clin Vaccine Immunol. 16: 1738-49.
  6. Bullers, S.J. et al. (2014) The human tissue-biomaterial interface: a role for PPARγ-dependent glucocorticoid receptor activation in regulating the CD163+ M2 macrophage phenotype.
    Tissue Eng Part A. 20: 2390-401.
  7. Mair, K.H. et al. (2013) Porcine CD8αdim/-NKp46high NK cells are in a highly activated state.
    Vet Res. 44: 13.
  8. Cruz, J.L. et al. (2013) Alphacoronavirus Protein 7 Modulates Host Innate Immune Response
    J Virol. 87: 9754-67.
  9. van Hout, G.P. et al. (2015) Invasive surgery reduces infarct size and preserves cardiac function in a porcine model of myocardial infarction.
    J Cell Mol Med. 19 (11): 2655-63.
  10. Toka, F.N. et al. (2009) Activation of porcine natural killer cells and lysis of foot-and-mouth disease virus infected cells.
    J Interferon Cytokine Res. 29 (3): 179-92.
  11. Dash, R. et al. (2018) Dose-Dependent Cardioprotection of Moderate (32°C) Versus Mild (35°C) Therapeutic Hypothermia in Porcine Acute Myocardial Infarction.
    JACC Cardiovasc Interv. 11 (2): 195-205.
  12. Talker, S.C. et al. (2015) Magnitude and kinetics of multifunctional CD4+ and CD8β+ T cells in pigs infected with swine influenza A virus.
    Vet Res. 46: 52.

Further Reading

  1. Piriou-Guzylack, L. (2008) Membrane markers of the immune cells in swine: an update.
    Vet Res. 39: 54.

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