CD107a antibody | 4E9/11
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|Mouse anti Pig CD107a, clone 4E9/11 recognizes porcine CD107a, a cell surface antigen, also known as lysosomal-associated membrane protein-1 or LAMP-1.
CD107a is a type 1 single pass transmembrane glycoprotein expressed on macrophages and more weakly on monocytes and granulocytes.
- Target Species
- Product Form
- Purified IgG - liquid
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Carrier Free
- Porcine alveolar macrophages.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised BALB/c mice were fused with cells of the mouse X63-Ag.8.653 myeloma cell line.
- For research purposes only
- 12 months from date of despatch
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
|Application Name||Verified||Min Dilution||Max Dilution|
|Flow Cytometry 1||1/25||1/50|
|Immunohistology - Frozen|
|Immunohistology - Paraffin 2|
- 1 Membrane permeabilization is required for this application. The use of Leucoperm (Product Code BUF09) is recommended for this purpose.
- 2 This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.
Sodium citrate buffer pH 6.0 is recommended for this purpose.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
- Histology Positive Control Tissue
- Porcine spleen.
References for CD107a antibody
Bullido, R. et al. (1997) Monoclonal antibodies specific for porcine monocytes/macrophages: macrophage heterogeneity in the pig evidenced by the expression of surface antigens.
Tissue Antigens. 49 (4): 403-13.
Carrillo, A. et al. (2002) Isolation and characterization of immortalized porcine aortic endothelial cell lines.
Vet Immunol Immunopathol. 89 (1-2): 91-8.
Domenech, N. et al. (2003) Identification of porcine macrophages with monoclonal antibodies in formalin-fixed, paraffin-embedded tissues.
Vet Immunol Immunopathol. 94 (1-2): 77-81.
Sánchez-Torres, C. et al. (2003) Expression of porcine CD163 on monocytes/macrophages correlates with permissiveness to African swine fever infection.
Arch Virol. 148 (12): 2307-23.
Toka, F.N. et al. (2009) Natural killer cell dysfunction during acute infection with foot-and-mouth disease virus.
Clin Vaccine Immunol. 16: 1738-49.
Bullers, S.J. et al. (2014) The human tissue-biomaterial interface: a role for PPARγ-dependent glucocorticoid receptor activation in regulating the CD163+ M2 macrophage phenotype.
Tissue Eng Part A. 20: 2390-401.
Mair, K.H. et al. (2013) Porcine CD8αdim/-NKp46high NK cells are in a highly activated state.
Vet Res. 44: 13.
Cruz, J.L. et al. (2013) Alphacoronavirus Protein 7 Modulates Host Innate Immune Response
J Virol. 87: 9754-67.
View The Latest Product References
van Hout, G.P. et al. (2015) Invasive surgery reduces infarct size and preserves cardiac function in a porcine model of myocardial infarction.
J Cell Mol Med. 19 (11): 2655-63.
Toka, F.N. et al. (2009) Activation of porcine natural killer cells and lysis of foot-and-mouth disease virus infected cells.
J Interferon Cytokine Res. 29 (3): 179-92.
Dash, R. et al. (2018) Dose-Dependent Cardioprotection of Moderate (32°C) Versus Mild (35°C) Therapeutic Hypothermia in Porcine Acute Myocardial Infarction.
JACC Cardiovasc Interv. 11 (2): 195-205.
Talker, S.C. et al. (2015) Magnitude and kinetics of multifunctional CD4+ and CD8β+ T cells in pigs infected with swine influenza A virus.
Vet Res. 46: 52.
Piriou-Guzylack, L. (2008) Membrane markers of the immune cells in swine: an update.
Vet Res. 39: 54.
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