VEGF antibody | CH-10

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Mouse anti Mouse VEGF

Product Type: Monoclonal Antibody
Clone: CH-10
Isotype: IgG1

Product Code	Applications	Datasheet	MSDS	Pack Size	List Price	Quantity
MCA5693	WB			0.1 mg

Summary
Secondary Antibodies

Mouse anti Mouse VEGF antibody, clone CH-10 recognises VEGF (vascular endothelial growth factor), a heparin-binding cytokine and member of the PDGF/VEGF growth factor family, which exists as several splice variants of the VEGF gene. VEGF, originally named vascular permeability factor due to its ability to act as vasodilator and increase microvascular permeability, is a potent stimulator of angiogenesis of both normal and cancerous cells, and acts as a regulator of vasculogenesis.

The functional forms of VEGF exist as disulphide-linked homodimers and are classified according to the number of amino acid residues within a constitutive polypeptide chain e.g VEGF165. All VEGF family members act through binding to the tyrosine kinase receptors VEGFR-1 (Flt-1) and VEGFR-2 (KDR/CD309), whilst the closely-related VEGF-C and VEGF-D, can also bind to VEGFR-3 (Flt-4).

Product Details

Target Species

Mouse

Species Cross-Reactivity

Target Species	Cross Reactivity
Human
Rat

N.B. Antibody reactivity and working conditions may vary between species.

Product Form

Purified IgG - liquid

Preparation

Purified IgG prepared by affinity chromatography

Buffer Solution

Phosphate buffered saline

Preservative Stabilisers

0.1% Sodium Azide (NaN₃)

Immunogen

Recombinant mouse VEGF.

Approx. Protein Concentrations

IgG concentration 1.0 mg/ml

Protein Molecular Weight

12/16/19 kDa

Storage Information

Storage: Store at +4^oC or at -20^oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 12 months from date of despatch

More Information

UniProt: Q00731 Related reagents
Entrez Gene: Vegfa Related reagents
GO Terms: GO:0005886 plasma membrane; GO:0001541 ovarian follicle development; GO:0001569 patterning of blood vessels; GO:0001701 in utero embryonic development; GO:0001822 kidney development; GO:0001938 positive regulation of endothelial cell proliferation; GO:0002053 positive regulation of mesenchymal cell proliferation; GO:0003007 heart morphogenesis; GO:0005615 extracellular space; GO:0006916 anti-apoptosis; GO:0040007 growth; GO:0007498 mesoderm development; GO:0007595 lactation; GO:0007610 behavior; GO:0008083 growth factor activity; GO:0008201 heparin binding; GO:0008283 cell proliferation; GO:0030855 epithelial cell differentiation; GO:0031077 post-embryonic camera-type eye development; GO:0042462 eye photoreceptor cell development; GO:0043117 positive regulation of vascular permeability; GO:0043129 surfactant homeostasis; GO:0048010 vascular endothelial growth factor receptor signaling pathway; GO:0048469 cell maturation; GO:0048593 camera-type eye morphogenesis; GO:0048739 cardiac muscle fiber development; GO:0048844 artery morphogenesis; GO:0051781 positive regulation of cell division; GO:0060319 primitive erythrocyte differentiation; GO:0060749 mammary gland alveolus development; GO:0090190 positive regulation of branching involved in ureteric bud morphogenesis
Regulatory: For research purposes only

Applications of VEGF antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Western Blotting

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Secondary Antibodies Available

Description	Product Code	Pack Size	Applications
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)	STAR117A	0.5 mg	E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)	STAR117D488GA	0.1 mg	F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed)	STAR117D549GA	0.1 mg	F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed)	STAR117D649GA	0.1 mg	F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)	STAR117D680GA	0.1 mg	F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)	STAR117D800GA	0.1 mg	F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)	STAR117F	0.5 mg	F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)	STAR117P	0.5 mg	E WB
Goat anti Mouse IgG (Fc):FITC	STAR120F	1 mg	C F
Goat anti Mouse IgG (Fc):HRP	STAR120P	1 mg	E WB
Rabbit F(ab')2 anti Mouse IgG:RPE	STAR12A	1 ml	F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)	STAR13B	1 mg	C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed)	STAR70	0.5 mg	F
Goat anti Mouse IgG:RPE (Rat Adsorbed)	STAR76	1 ml	F
Goat anti Mouse IgG:HRP (Rat Adsorbed)	STAR77	0.5 mg	C E P
Goat anti Mouse IgG/A/M:Alk. Phos.	STAR87A	1 mg	C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)	STAR87P	1 mg	E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800	STAR8D800GA	0.1 mg	F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC	STAR9B	1 mg	F

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