VEGF antibody | CH-10
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Mouse anti Mouse VEGF antibody, clone CH-10 recognises VEGF (vascular endothelial growth factor), a heparin-binding cytokine and member of the PDGF/VEGF growth factor family, which exists as several splice variants of the VEGF gene. VEGF, originally named vascular permeability factor due to its ability to act as vasodilator and increase microvascular permeability, is a potent stimulator of angiogenesis of both normal and cancerous cells, and acts as a regulator of vasculogenesis.
The functional forms of VEGF exist as disulphide-linked homodimers and are classified according to the number of amino acid residues within a constitutive polypeptide chain e.g VEGF165. All VEGF family members act through binding to the tyrosine kinase receptors VEGFR-1 (Flt-1) and VEGFR-2 (KDR/CD309), whilst the closely-related VEGF-C and VEGF-D, can also bind to VEGFR-3 (Flt-4).
The functional forms of VEGF exist as disulphide-linked homodimers and are classified according to the number of amino acid residues within a constitutive polypeptide chain e.g VEGF165. All VEGF family members act through binding to the tyrosine kinase receptors VEGFR-1 (Flt-1) and VEGFR-2 (KDR/CD309), whilst the closely-related VEGF-C and VEGF-D, can also bind to VEGFR-3 (Flt-4).
Product Details
- Target Species
- Mouse
- Species Cross-Reactivity
-
Target Species Cross Reactivity Human Rat - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.1% Sodium Azide (NaN3)
- Immunogen
- Recombinant mouse VEGF.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Protein Molecular Weight
- 12/16/19 kDa
Storage Information
- Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- Q00731
- Entrez Gene
- Vegfa
- GO Terms
- GO:0005886 plasma membrane
- GO:0001541 ovarian follicle development
- GO:0001569 patterning of blood vessels
- GO:0001701 in utero embryonic development
- GO:0001822 kidney development
- GO:0001938 positive regulation of endothelial cell proliferation
- GO:0002053 positive regulation of mesenchymal cell proliferation
- GO:0003007 heart morphogenesis
- GO:0005615 extracellular space
- GO:0006916 anti-apoptosis
- GO:0040007 growth
- GO:0007498 mesoderm development
- GO:0007595 lactation
- GO:0007610 behavior
- GO:0008083 growth factor activity
- GO:0008201 heparin binding
- GO:0008283 cell proliferation
- GO:0030855 epithelial cell differentiation
- GO:0031077 post-embryonic camera-type eye development
- GO:0042462 eye photoreceptor cell development
- GO:0043117 positive regulation of vascular permeability
- GO:0043129 surfactant homeostasis
- GO:0048010 vascular endothelial growth factor receptor signaling pathway
- GO:0048469 cell maturation
- GO:0048593 camera-type eye morphogenesis
- GO:0048739 cardiac muscle fiber development
- GO:0048844 artery morphogenesis
- GO:0051781 positive regulation of cell division
- GO:0060319 primitive erythrocyte differentiation
- GO:0060749 mammary gland alveolus development
- GO:0090190 positive regulation of branching involved in ureteric bud morphogenesis
- Regulatory
- For research purposes only
Applications of VEGF antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
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Secondary Antibodies Available
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