MHC Class I antibody | 2G5
Mouse anti Mouse MHC Class I antibody, clone 2G5 (MCA2189) used for the evaluation of MHC class I expression on guinea pig lung infiltrates in a mycobacterial disease model by flow cytometry.
Image caption:
Evaluation of neutrophil infiltration and MHC expressions in the infected lung. The expression of MHC I, MHC II and percentage of neutrophils were monitored at different time points during the course of therapy. The bar diagram shows the mean percentage of MHC expression on gated population or neutrophil content in the lungs of drug or drug plus MIP group. Data represents the mean value of four animals in each group. * p<0.05. Entire lymphocyte negative region was gated based on forward versus side scatter and MHC expression or neutrophil infiltration was analyzed on the gated region. The representative plots show the MHC expression or neutrophil infiltration in different experimental groups along with the suitable negative and positive controls. Percentage value has been mentioned in the assigned region in each plot.
From: Gupta A, Ahmad FJ, Ahmad F, Gupta UD, Natarajan M, Katoch V, et al. (2012)
Efficacy of Mycobacterium indicus pranii Immunotherapy as an Adjunct to Chemotherapy for Tuberculosis and Underlying Immune Responses in the Lung.
PLoS ONE 7(7): e39215.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
FITC conjugated Mouse anti Mouse MHC Class I antibody, clone 2G5 (MCA2189F) used for the evaluation of MHC class I expression on murine retinal epithelial cells by flow cytometry.
Image caption:
Functional characteristics of mouse iPS-RPE.
(a and b) Two types of pRPE were compared with mouse iPS-RPE. Phase-contrast images of 2 week-cultured pRPE obtained from adult mice (pRPE (2w), a) and 2 day-cultured pRPE obtained from PN day10-mice (pRPE (2d), b) are shown. (c) The expression of RPE functional marker mRNAs in fresh PN day10-RPE, DD29 iPS-RPE, pRPE (2d), and pRPE (2w) is shown. The data for fresh RPE and iPS-RPE are the same as those shown in Fig 4. (d and e) Rod outer segment phagocytosis assay. Phase-contrast image and fluorescent image of iPS-RPE that was co-cultured with or without FITC-ROS and washed by medium are shown (d). Both iPS-RPE and pRPE (2w) phagocytosed FITC-ROS, and the percentage of FITC-positive cells in iPS-RPE was significantly higher than that in pRPE (2w). Tukey-Kramer test. n = 3. (f) Immune surface antigen expression evaluated by flow cytometry. The red line histograms represent isotype control. Numbers in the histogram indicate the percentage of positive cells. (g and h) Percentage of Ki-67 and CD4 double-positive T cells (g) and that of Ki-67 and CD8 double-positive T cells (h) after stimulation by anti-CD3 and anti-CD28 antibody. iPS-RPE significantly suppressed the proliferation of CD4-positive T cells and CD8-positive T cells. Tukey-Kramer test. n = 3. Scale bars: 200 μm (a, b) and 100 μm (d). **: p<0.01, *: p<0.05.
From: Iwasaki Y, Sugita S, Mandai M, Yonemura S, Onishi A, Ito S-i, et al. (2016)
Differentiation/Purification Protocol for Retinal Pigment Epithelium from Mouse Induced Pluripotent Stem Cells as a Research Tool.
PLoS ONE 11(7): e0158282.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Alexa Fluor 647 conjugated Mouse anti Mouse MHC Class I antibody, clone 2G5 (MCA2189A647) used to assess MHC Class I expression on goat monocytes by flow cytometry.
Image caption:
rFg-CaBP4-mediated differential expression of major histocompatibility complex (MHC) on the surface of goat monocytes. Monocytes were cultured in the presence of the indicated concentrations of rFg-CaBP4 and control buffer for 24 h. MHC class-I (MHC-I) and MHC-II expressions were measured by flow cytometric analysis and calculated as the percentage of mean fluorescence intensity (MFI). The data are presented as the MFI ± SD from three independent experiments. **P <0.01, ***P <0.001, ****P <0.0001, ns (compared to untreated control)
From: Ehsan M, Hu RS, Hou JL, Elsheikha HM, Li XD, Liang PH, Zhu XQ.
Fasciola gigantica tegumental calcium-binding EF-hand protein 4 exerts immunomodulatory effects on goat monocytes.
Parasit Vectors. 2021 May 22;14(1):276.
doi: 10.1186/s13071-021-04784-5.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
A647 conjugated Mouse anti Mouse MHC Class I antibody, clone 2G5 (MCA2189A647) used to evaluate MHC Class I expression in goat monocytes by flow cytometry.
Image caption:
Inhibitory effect of recombinant rhodanese protein from Haemonchus contortus (rHCRD) on the expression of MHC-II by goat monocytes. Cells were cultured in the presence of varies rHCRD concentrations and his-tagged protein or control buffer (PBS/DTT) for 24 h. The cells treated with LPS were used as positive control. MHC-II expression was analysed on flow cytometric analysis and calculated as the percentage of mean fluorescence intensity (MFI) of controls. Bars represent the MFI ± SD of controls. The data presented are results of three independent experiments (*P <0.05, **P <0.01, ***P <0.001). a MHC-I. b MHC-II
From: Wang Y, Ehsan M, Huang J, Aimulajiang K, Yan R, Song X, Xu L, Li X.
Characterization of a rhodanese homologue from Haemonchus contortus and its immune-modulatory effects on goat immune cells in vitro.
Parasit Vectors. 2020 Sep 7;13(1):454.
doi: 10.1186/s13071-020-04333-6.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Mouse MHC Class I
- Product Type
- Monoclonal Antibody
- Clone
- 2G5
- Isotype
- IgG2b
- Specificity
- MHC Class I
Antibody Quality and Performance Guarantee
| Mouse anti Mouse MHC Class I antibody, clone 2G5 recognizes a monomorphic epitope present on murine MHC class I molecules, expressed at varying levels on the majority of nucleated cells. The major histocompatibility complex (MHC) is a cluster of genes that are important in the immune response to infections. In mice, this complex is referred to as the histocompatibility 2 (H-2) region. The epitope recognized by clone 2G5 is conformation dependent and is reported to be phylogenetically conserved (Claesson et al. 1994). Reactivity has been observed with some canine samples suggesting that this antibody may recognize a polymorphic epitope of canine MHC class I. |
- Target Species
- Mouse
- Species Cross-Reactivity
-
Target Species Cross Reactivity Rat Guinea Pig Sheep Bovine Pig Human Hamster - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% sodium azide (NaN3)
- Carrier Free
- Yes
- Immunogen
- Purified H-2Kb and H-2Db MHC-I molecules.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunized C1D mice were fused with cells of the X63 myeloma cell line.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
1/10 | 1/25 |
| Immunoprecipitation | |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10μl of the suggested working dilution to label 106 cells in 100μl
Source Reference
-
Claesson, M.H. et al. (1994) Antibodies directed against monomorphic and evolutionary conserved self epitopes may be generated in 'knock-out' mice. Development of monoclonal antibodies directed against monomorphic MHC class I determinants.
Scand J Immunol. 40 (2): 257-64.
References for MHC Class I antibody
-
Cenci, E. et al. (2006) Modulation of phenotype and function of dendritic cells by a therapeutic synthetic killer peptide.
J Leukoc Biol. 79 (1): 40-5. -
Perone, M.J. et al. (2006) Dendritic cells expressing transgenic galectin-1 delay onset of autoimmune diabetes in mice.
J Immunol. 177 (8): 5278-89. -
Giunchetti, R.C. et al. (2007) Immunogenicity of a killed Leishmania vaccine with saponin adjuvant in dogs.
Vaccine. 25 (44): 7674-86. -
Huang, Y.C. et al. (2008) CD5-low expression lymphocytes in canine peripheral blood show characteristics of natural killer cells.
J Leukoc Biol. 84 (6): 1501-10. -
Liu, C.C. et al. (2008) Transient downregulation of monocyte-derived dendritic-cell differentiation, function, and survival during tumoral progression and regression in an in vivo canine model of transmissible venereal tumor.
Cancer Immunol Immunother. 57 (4): 479-91. -
Letellier, M. et al. (2008) Normal adult climbing fiber monoinnervation of cerebellar Purkinje cells in mice lacking MHC class I molecules.
Dev Neurobiol. 68 (8): 997-1006. -
Giunchetti RC et al. (2008) A killed Leishmania vaccine with sand fly saliva extract and saponin adjuvant displays immunogenicity in dogs.
Vaccine. 26 (5): 623-38. -
Vitadello, M. et al. (2010) Myofiber stress-response in myositis: parallel investigations on patients and experimental animal models of muscle regeneration and systemic inflammation.
Arthritis Res Ther. 12 (2): R52.
View The Latest Product References
-
Gupta, A. et al. (2012) Efficacy of Mycobacterium indicus pranii immunotherapy as an adjunct to chemotherapy for tuberculosis and underlying immune responses in the lung.
PLoS One. 7 (7): e39215. -
Patel, G.K. et al. (2012) A humanized stromal bed is required for engraftment of isolated human primary squamous cell carcinoma cells in immunocompromised mice.
J Invest Dermatol. 132 (2): 284-90. -
Gupta, A. et al. (2012) Protective efficacy of Mycobacterium indicus pranii against tuberculosis and underlying local lung immune responses in guinea pig model.
Vaccine. 30 (43): 6198-209. -
Zuza, A.L. et al. (2016) Astrocyte response to St. Louis encephalitis virus.
Virus Res. 217: 92-100. -
Reid E et al. (2016) Type I and III IFNs Produced by Plasmacytoid Dendritic Cells in Response to a Member of the Flaviviridae Suppress Cellular Immune Responses.
J Immunol. 196 (10): 4214-26. -
Iwasaki, Y. et al. (2016) Differentiation/Purification Protocol for Retinal Pigment Epithelium from Mouse Induced Pluripotent Stem Cells as a Research Tool.
PLoS One. 11 (7): e0158282. -
Wang, Y. et al. (2020) Characterization of a rhodanese homologue from Haemonchus contortus and its immune-modulatory effects on goat immune cells in vitro.
Parasit Vectors. 13 (1): 454. -
Ehsan, M. et al. (2021) Fasciola gigantica tegumental calcium-binding EF-hand protein 4 exerts immunomodulatory effects on goat monocytes.
Parasit Vectors. 14 (1): 276. -
Wang, Y. et al. (2020) Modulatory functions of recombinant electron transfer flavoprotein α subunit protein from Haemonchus contortus on goat immune cells in vitro.
Vet Parasitol. 288: 109300.
Further Reading
-
Piriou-Guzylack, L. (2008) Membrane markers of the immune cells in swine: an update.
Vet Res. 39: 54.
- RRID
- AB_324082
MCA2189
149802
159439
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