MHC Class I antibody | 2G5

Mouse anti Mouse MHC Class I

Product Type
Monoclonal Antibody
Clone
2G5
Isotype
IgG2b
Specificity
MHC Class I

Product Code Applications Pack Size List Price Your Price Qty
MCA2189
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F IP 0.25 mg loader
List Price Your Price
loader
Search for Batch Specific Datasheets

Mouse anti Mouse MHC Class I antibody, clone 2G5 recognizes a monomorphic epitope present on murine MHC class I molecules, expressed at varying levels on the majority of nucleated cells. The major histocompatibility complex (MHC) is a cluster of genes that are important in the immune response to infections. In mice, this complex is referred to as the histocompatibility 2 (H-2) region.

The epitope recognized by clone 2G5 is conformation dependent and is reported to be phylogenetically conserved (Claesson et al. 1994). Reactivity has been observed with some canine samples suggesting that this antibody may recognize a polymorphic epitope of canine MHC class I.

Target Species
Mouse
Species Cross-Reactivity
Target SpeciesCross Reactivity
Rat
Guinea Pig
Sheep
Bovine
Pig
Human
Hamster
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Carrier Free
Yes
Immunogen
Purified H-2Kb and H-2Db MHC-I molecules.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised C1D mice were fused with cells of the X63 myeloma cell line.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/10 1/25
Immunoprecipitation
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative\positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.

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Source Reference

  1. Claesson, M.H. et al. (1994) Antibodies directed against monomorphic and evolutionary conserved self epitopes may be generated in 'knock-out' mice. Development of monoclonal antibodies directed against monomorphic MHC class I determinants.
    Scand J Immunol. 40 (2): 257-64.

References for MHC Class I antibody

  1. Perone, M.J. et al. (2006) Dendritic cells expressing transgenic galectin-1 delay onset of autoimmune diabetes in mice.
    J Immunol. 177 (8): 5278-89.
  2. Vitadello, M. et al. (2010) Myofiber stress-response in myositis: parallel investigations on patients and experimental animal models of muscle regeneration and systemic inflammation.
    Arthritis Res Ther. 12 (2): R52.
  3. Huang, Y.C. et al. (2008) CD5-low expression lymphocytes in canine peripheral blood show characteristics of natural killer cells.
    J Leukoc Biol. 84 (6): 1501-10.
  4. Liu, C.C. et al. (2008) Transient downregulation of monocyte-derived dendritic-cell differentiation, function, and survival during tumoral progression and regression in an in vivo canine model of transmissible venereal tumor.
    Cancer Immunol Immunother. 57 (4): 479-91.
  5. Letellier, M. et al. (2008) Normal adult climbing fiber monoinnervation of cerebellar Purkinje cells in mice lacking MHC class I molecules.
    Dev Neurobiol. 68 (8): 997-1006.
  6. Gupta, A. et al. (2012) Efficacy of Mycobacterium indicus pranii immunotherapy as an adjunct to chemotherapy for tuberculosis and underlying immune responses in the lung.
    PLoS One. 7 (7): e39215.
  7. Giunchetti, R.C. et al. (2007) Immunogenicity of a killed Leishmania vaccine with saponin adjuvant in dogs.
    Vaccine. 25 (44): 7674-86.
  8. Cenci, E. et al. (2006) Modulation of phenotype and function of dendritic cells by a therapeutic synthetic killer peptide.
    J Leukoc Biol. 79 (1): 40-5.
  9. View The Latest Product References
  10. Giunchetti RC et al. (2008) A killed Leishmania vaccine with sand fly saliva extract and saponin adjuvant displays immunogenicity in dogs.
    Vaccine. 26 (5): 623-38.
  11. Patel, G.K. et al. (2012) A humanized stromal bed is required for engraftment of isolated human primary squamous cell carcinoma cells in immunocompromised mice.
    J Invest Dermatol. 132 (2): 284-90.
  12. Zuza, A.L. et al. (2016) Astrocyte response to St. Louis encephalitis virus.
    Virus Res. 217: 92-100.
  13. Lohan, P. et al. (2016) Culture expanded primary chondrocytes have potent immunomodulatory properties and do not induce an allogeneic immune response.
    Osteoarthritis Cartilage. 24 (3): 521-33.
  14. Gupta, A. et al. (2012) Protective efficacy of Mycobacterium indicus pranii against tuberculosis and underlying local lung immune responses in guinea pig model.
    Vaccine. 30 (43): 6198-209.
  15. Reid E et al. (2016) Type I and III IFNs Produced by Plasmacytoid Dendritic Cells in Response to a Member of the Flaviviridae Suppress Cellular Immune Responses.
    J Immunol. 196 (10): 4214-26.
  16. Iwasaki, Y. et al. (2016) Differentiation/Purification Protocol for Retinal Pigment Epithelium from Mouse Induced Pluripotent Stem Cells as a Research Tool.
    PLoS One. 11 (7): e0158282.
  17. Wang, Y. et al. (2020) Characterization of a rhodanese homologue from Haemonchus contortus and its immune-modulatory effects on goat immune cells in vitro.
    Parasit Vectors. 13 (1): 454.
  18. Ehsan, M. et al. (2021) Fasciola gigantica tegumental calcium-binding EF-hand protein 4 exerts immunomodulatory effects on goat monocytes.
    Parasit Vectors. 14 (1): 276.

Further Reading

  1. Piriou-Guzylack, L. (2008) Membrane markers of the immune cells in swine: an update.
    Vet Res. 39: 54.

Immunofluorescence

RRID
AB_324082
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