CD44 antibody | 5035-41.1D

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Mouse anti Mouse CD44:FITC

Mouse anti Mouse CD44

Product Type
Monoclonal Antibody
Clone
5035-41.1D
Isotype
IgG1
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA1014FT F 25 µg
MCA1014F F 0.1 mg
MCA1014FB F 0.5 mg
MCA1014G F IP 0.25 mg
Mouse anti Human CD44 antibody, clone 5035-41.1D recognizes human CD44, also known as Lymphocyte antigen 24, Extracellular matrix receptor III, GP90 lymphocyte homing/adhesion receptor, HUTCH-I, Phagocytic glycoprotein I (PGP-1), Hermes antigen or Hyaluronate receptor. CD44 is found on >90% bone marrow cells and spleen cells, 56% of thymus cells and 90% of lymph node cells.

Mouse anti Human CD44 antibody, clone 5035-41.1D recognizes the following strains of mice:- C57BL/6, C57BL/10, C57/L, C58A, AKR, 129, SJL, NZB, C3H, CE, CBA/H.

Product Details

Target Species
Mouse
Product Form
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
Preservative Stabilisers
0.09%Sodium Azide
Carrier Free
Yes
Immunogen
Spleen cells from B6PL-Ly-2a / Ly-3a B6-Ly-1a mice.
Approx. Protein Concentrations
Pack Size: 25 µg, 0.1 mg
IgG concentration 0.1mg/ml
Pack Size: 0.5 mg
IgG concentration 0.5 mg/ml
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunized mice were fused with cells of the mouse P3-NS1-1-Ag4 myeloma cell line.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC for one month or at -20oC for longer.

This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
18 months from date of despatch.
Guarantee
18 months from date of despatch.

More Information

UniProt
P15379 Related reagents
Entrez Gene
Cd44 Related reagents
Regulatory
For research purposes only

Applications of CD44 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/10 Neat Pack Size: 25 µg, 0.1 mg
1/50 Pack Size: 0.5 mg
Flow Cytometry 1/50 1/100
Immunoprecipitation
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells or 100ul whole blood
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells or 100ul whole blood

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
Mouse Seroblock FcR BUF041A 0.1 mg F
Mouse Seroblock FcR BUF041B 0.5 mg F

Application Based External Images

Flow Cytometry

Product Specific References

References for CD44 antibody

  1. Sutton, V.R. et al. (1987) Genetic and biochemical characterization of antigens encoded by the Ly24 (Pgp-1) locus.
    J. Immunogenetics 14: 43-57.
  2. Ariel, A. et al. (2001) Cell surface-expressed moesin-like receptor regulates T cell interactions with tissue components and binds an adhesion-modulating IL-2 peptide generated by elastase.
    J Immunol. 166: 3052-60.
  3. Ishizuka, T. et al. (2011) Superoxide induced by a high-glucose concentration attenuates production of angiogenic growth factors in hypoxic mouse mesenchymal stem cells.
    J Endocrinol. 208: 147-59.
  4. Qian, K. et al. (2015) Effects of Tanshinone IIA on osteogenic differentiation of mouse bone marrow mesenchymal stem cells.
    Naunyn Schmiedebergs Arch Pharmacol. 388 (11): 1201-9.
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