CD44 antibody | 5035-41.1D
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F ResetMouse anti Mouse CD44
- Product Type
- Monoclonal Antibody
- Clone
- 5035-41.1D
- Isotype
- IgG1
- Specificity
- CD44
Antibody Quality and Performance Guarantee
| Mouse anti Mouse CD44 antibody, clone 5035-41.1D recognizes human CD44, also known as Lymphocyte antigen 24, Extracellular matrix receptor III, GP90 lymphocyte homing/adhesion receptor, HUTCH-I, Phagocytic glycoprotein I (PGP-1), Hermes antigen or Hyaluronate receptor. CD44 is found on >90% bone marrow cells and spleen cells, 56% of thymus cells and 90% of lymph node cells. Mouse anti Mouse CD44 antibody, clone 5035-41.1D recognizes the following strains of mice:- C57BL/6, C57BL/10, C57/L, C58A, AKR, 129, SJL, NZB, C3H, CE, CBA/H. |
- Target Species
- Mouse
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide - Carrier Free
- Yes
- Immunogen
- Spleen cells from B6PL-Ly-2a / Ly-3a B6-Ly-1a mice.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunized mice were fused with cells of the mouse P3-NS1-1-Ag4 myeloma cell line.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
1/50 | 1/100 |
| Immunoprecipitation | |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells or 100ul whole blood
Source Reference
-
Sutton, V.R. et al. (1987) Genetic and biochemical characterization of antigens encoded by the Ly-24 (Pgp-1) locus.
J Immunogenet. 14 (1): 43-57.
References for CD44 antibody
-
A, A. et al. (2001) Cell surface-expressed moesin-like receptor regulates T cell interactions with tissue components and binds an adhesion-modulating IL-2 peptide generated by elastase.
J Immunol. 166 (5): 3052-60. -
Qian, K. et al. (2015) Effects of Tanshinone IIA on osteogenic differentiation of mouse bone marrow mesenchymal stem cells.
Naunyn Schmiedebergs Arch Pharmacol. 388 (11): 1201-9.
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0312R
157506
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If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
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