CD44 antibody | 5035-41.1D

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Anti Mouse CD44 Antibody, clone 5035-41.1D thumbnail image 1

Anti Mouse CD44 Antibody, clone 5035-41.1D thumbnail image 2

Anti Mouse CD44 Antibody, clone 5035-41.1D gallery image 1 — Figure A. RPE conjugated rat anti mouse CD3 (MCA500PE) and FITC conjugated mouse IgG1 isotype control (MCA1209F). Figure B. RPE conjugated rat anti mouse CD3 (MCA500PE) and FITC conjugated mouse anti mouse CD44 (MCA1014F). All experiments performed on red cell lysed murine splenocytes gated on lymphocytes in the presence of murine SeroBlock (BUF041A).

Anti Mouse CD44 Antibody, clone 5035-41.1D gallery image 2 — Figure A. APC conjugated rat anti mouse CD3 (MCA500APC) and FITC conjugated mouse IgG1 isotype control (MCA1209F). Figure B. APC conjugated rat anti mouse CD3 (MCA500APC) and FITC conjugated mouse anti mouse CD44 (MCA1014F). All experiments performed on murine splenocytes in the presence of murine SeroBlock (BUF041A).

Mouse anti Mouse CD44:FITC

Mouse anti Mouse CD44

Product Type: Monoclonal Antibody
Clone: 5035-41.1D
Isotype: IgG1

Product Code	Applications	Pack Size
MCA1014FT	F	25 µg
MCA1014F	F	0.1 mg
MCA1014FB	F	0.5 mg
MCA1014G	F IP	0.25 mg

Mouse anti Human CD44 antibody, clone 5035-41.1D recognizes human CD44, also known as Lymphocyte antigen 24, Extracellular matrix receptor III, GP90 lymphocyte homing/adhesion receptor, HUTCH-I, Phagocytic glycoprotein I (PGP-1), Hermes antigen or Hyaluronate receptor. CD44 is found on >90% bone marrow cells and spleen cells, 56% of thymus cells and 90% of lymph node cells.

Mouse anti Human CD44 antibody, clone 5035-41.1D recognizes the following strains of mice:- C57BL/6, C57BL/10, C57/L, C58A, AKR, 129, SJL, NZB, C3H, CE, CBA/H.

Product Details

Target Species

Mouse

Product Form

Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid

Product Form

Purified IgG - liquid

Preparation

Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant

Preparation

Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant

Buffer Solution

Phosphate buffered saline

Buffer Solution

Phosphate buffered saline

Preservative Stabilisers

0.09%	Sodium Azide
1%	Bovine Serum Albumin

Preservative Stabilisers

0.09%

Sodium Azide

Carrier Free

Yes

Immunogen

Spleen cells from B6PL-Ly-2a / Ly-3a B6-Ly-1a mice.

Approx. Protein Concentrations

Pack Size: 25 µg, 0.1 mg

IgG concentration 0.1mg/ml

Pack Size: 0.5 mg

IgG concentration 0.5 mg/ml

Approx. Protein Concentrations

IgG concentration 1.0 mg/ml

Fusion Partners

Spleen cells from immunized mice were fused with cells of the mouse P3-NS1-1-Ag4 myeloma cell line.

Storage Information

Storage: Store at +4^oC or at -20^oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage: Store at +4^oC for one month or at -20^oC for longer.

This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee: 18 months from date of despatch.
Guarantee: 18 months from date of despatch.

More Information

UniProt: P15379 Related reagents
Entrez Gene: Cd44 Related reagents
Regulatory: For research purposes only

Applications of CD44 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Min Dilution	Max Dilution
Flow Cytometry	1/10	Neat Pack Size: 25 µg, 0.1 mg 1/50 Pack Size: 0.5 mg
Flow Cytometry	1/50	1/100
Immunoprecipitation

Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Flow Cytometry: Use 10ul of the suggested working dilution to label 10⁶ cells or 100ul whole blood
Flow Cytometry: Use 10ul of the suggested working dilution to label 10⁶ cells or 100ul whole blood

Secondary Antibodies Available

Description	Product Code	Pack Size	Applications
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)	STAR117A	0.5 mg	E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)	STAR117D488GA	0.1 mg	F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed)	STAR117D549GA	0.1 mg	F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed)	STAR117D649GA	0.1 mg	F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)	STAR117D680GA	0.1 mg	F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)	STAR117D800GA	0.1 mg	F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)	STAR117F	0.5 mg	F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)	STAR117P	0.5 mg	E WB
Goat anti Mouse IgG (Fc):FITC	STAR120F	1 mg	C F
Goat anti Mouse IgG (Fc):HRP	STAR120P	1 mg	E WB
Rabbit F(ab')2 anti Mouse IgG:RPE	STAR12A	1 ml	F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)	STAR13B	1 mg	C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed)	STAR70	0.5 mg	F
Goat anti Mouse IgG:RPE (Rat Adsorbed)	STAR76	1 ml	F
Goat anti Mouse IgG:HRP (Rat Adsorbed)	STAR77	0.5 mg	C E P
Goat anti Mouse IgG/A/M:Alk. Phos.	STAR87A	1 mg	C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)	STAR87P	1 mg	E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800	STAR8D800GA	0.1 mg	F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC	STAR9B	1 mg	F

Useful Reagents Available

Description	Product Code	Pack Size	Applications	List Price	Quantity
Mouse Seroblock FcR	BUF041A	0.1 mg	F
Mouse Seroblock FcR	BUF041B	0.5 mg	F

Application Based External Images

Flow Cytometry

Product Specific References

References for CD44 antibody

Sutton, V.R. et al. (1987) Genetic and biochemical characterization of antigens encoded by the Ly24 (Pgp-1) locus.
J. Immunogenetics 14: 43-57.
Ariel, A. et al. (2001) Cell surface-expressed moesin-like receptor regulates T cell interactions with tissue components and binds an adhesion-modulating IL-2 peptide generated by elastase.
J Immunol. 166: 3052-60.
Ishizuka, T. et al. (2011) Superoxide induced by a high-glucose concentration attenuates production of angiogenic growth factors in hypoxic mouse mesenchymal stem cells.
J Endocrinol. 208: 147-59.
Qian, K. et al. (2015) Effects of Tanshinone IIA on osteogenic differentiation of mouse bone marrow mesenchymal stem cells.
Naunyn Schmiedebergs Arch Pharmacol. 388 (11): 1201-9.

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