CD309 antibody | CH-11

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Mouse anti CD309

Product Type
Monoclonal Antibody
Clone
CH-11
Isotype
IgG1
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
OBT1170 E P* WB 0.1 mg
Mouse anti CD309, Clone CH-11, recognizes both mouse and human vascular endothelial growth factor receptor 2 (VEGF R2), also known as FLK-1 or Kinase insert domain receptor. CD309 is one of three VEGF receptor kinases which play an important role in angiogenesis. VEGF receptors are characterised by a cytoplasmic split-kinase domain and seven extracellular immunoglobulin domains.

Product Details

Target Species
Mouse
Species Cross-Reactivity
Target SpeciesCross Reactivity
Human
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.1% Sodium azide
Immunogen
Recombinant fragment corresponding to amino acids 1158 to 1345 within the intracellular region of mouse CD309.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch.

More Information

UniProt
P35918 Related reagents
P35968 Related reagents
Entrez Gene
Kdr Related reagents
KDR Related reagents
GO Terms
GO:0001525 angiogenesis
GO:0001541 ovarian follicle development
GO:0001570 vasculogenesis
GO:0001945 lymph vessel development
GO:0002053 positive regulation of mesenchymal cell proliferation
GO:0019838 growth factor binding
GO:0005021 vascular endothelial growth factor receptor activity
GO:0005887 integral to plasma membrane
GO:0005524 ATP binding
GO:0006468 protein phosphorylation
GO:0016477 cell migration
GO:0030097 hemopoiesis
GO:0043129 surfactant homeostasis
GO:0045165 cell fate commitment
GO:0045446 endothelial cell differentiation
GO:0048286 lung alveolus development
GO:0048469 cell maturation
GO:0048754 branching morphogenesis of a tube
GO:0055074 calcium ion homeostasis
GO:0001938 positive regulation of endothelial cell proliferation
GO:0004716 receptor signaling protein tyrosine kinase activity
GO:0005178 integrin binding
GO:0008360 regulation of cell shape
GO:0051879 Hsp90 protein binding
GO:0010595 positive regulation of endothelial cell migration
GO:0044419 interspecies interaction between organisms
GO:0050927 positive regulation of positive chemotaxis
GO:0051894 positive regulation of focal adhesion assembly
Regulatory
For research purposes only

Applications of CD309 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Immunohistology - Paraffin 1
Western Blotting
  1. 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.EDTA pH8.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Histology Positive Control Tissue
Placenta
Western Blotting
Clone CH-11 detects bands of approximately 200kDa and 230kDa in HUVEC cell lysates.

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
Antigen Retrieval Buffer, pH8.0 BUF025A 500 ml P
Antigen Retrieval Buffer, pH8.0 BUF025C 50 ml P

Product Specific References

References for CD309 antibody

  1. Malini, E. et al. (2015) Role of LIMP-2 in the intracellular trafficking of β-glucosidase in different human cellular models.
    FASEB J. 29 (9): 3839-52.
  2. Dardis, A. et al. (2016) Altered localization and functionality of TAR DNA Binding Protein 43 (TDP-43) in niemann- pick disease type C.
    Acta Neuropathol Commun. 4 (1): 52.
  3. Qiang, L. et al. (2013) Expression of miR-126 and miR-508-5p in endothelial progenitor cells is associated with the prognosis of chronic heart failure patients.
    Int J Cardiol. 168 (3): 2082-8.
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