CD309 antibody | CH-11
Mouse anti CD309, Clone CH-11, recognizes both mouse and human vascular endothelial growth factor receptor 2 (VEGF R2), also known as FLK-1 or Kinase insert domain receptor. CD309 is one of three VEGF receptor kinases which play an important role in angiogenesis. VEGF receptors are characterised by a cytoplasmic split-kinase domain and seven extracellular immunoglobulin domains.
- Target Species
- Species Cross-Reactivity
Target Species Cross Reactivity Human
- N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Purified IgG prepared by affinity chromatography on Protein A
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.1% Sodium azide
- Recombinant fragment corresponding to amino acids 1158 to 1345 within the intracellular region of mouse CD309.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
- 12 months from date of despatch.
- P35918 Related reagents
- P35968 Related reagents
- Entrez Gene
- Kdr Related reagents
- KDR Related reagents
- GO Terms
- GO:0001525 angiogenesis
- GO:0001541 ovarian follicle development
- GO:0001570 vasculogenesis
- GO:0001945 lymph vessel development
- GO:0002053 positive regulation of mesenchymal cell proliferation
- GO:0019838 growth factor binding
- GO:0005021 vascular endothelial growth factor receptor activity
- GO:0005887 integral to plasma membrane
- GO:0005524 ATP binding
- GO:0006468 protein phosphorylation
- GO:0016477 cell migration
- GO:0030097 hemopoiesis
- GO:0043129 surfactant homeostasis
- GO:0045165 cell fate commitment
- GO:0045446 endothelial cell differentiation
- GO:0048286 lung alveolus development
- GO:0048469 cell maturation
- GO:0048754 branching morphogenesis of a tube
- GO:0055074 calcium ion homeostasis
- GO:0001938 positive regulation of endothelial cell proliferation
- GO:0004716 receptor signaling protein tyrosine kinase activity
- GO:0005178 integrin binding
- GO:0008360 regulation of cell shape
- GO:0051879 Hsp90 protein binding
- GO:0010595 positive regulation of endothelial cell migration
- GO:0044419 interspecies interaction between organisms
- GO:0050927 positive regulation of positive chemotaxis
- GO:0051894 positive regulation of focal adhesion assembly
- For research purposes only
Applications of CD309 antibody
|Application Name||Verified||Min Dilution||Max Dilution|
|Immunohistology - Paraffin 1|
- 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.EDTA pH8.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Histology Positive Control Tissue
- Western Blotting
- Clone CH-11 detects bands of approximately 200kDa and 230kDa in HUVEC cell lysates.
Copyright © 2019 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
Useful Reagents Available
|Description||Product Code||Pack Size||Applications||List Price||Quantity|
|Antigen Retrieval Buffer, pH8.0||BUF025A||500 ml||P|
|Antigen Retrieval Buffer, pH8.0||BUF025C||50 ml||P|
Product Specific References
References for CD309 antibody
Malini, E. et al. (2015) Role of LIMP-2 in the intracellular trafficking of β-glucosidase in different human cellular models.
FASEB J. 29 (9): 3839-52.
Dardis, A. et al. (2016) Altered localization and functionality of TAR DNA Binding Protein 43 (TDP-43) in niemann- pick disease type C.
Acta Neuropathol Commun. 4 (1): 52.
Qiang, L. et al. (2013) Expression of miR-126 and miR-508-5p in endothelial progenitor cells is associated with the prognosis of chronic heart failure patients.
Int J Cardiol. 168 (3): 2082-8.