CD161 / NK1.1 antibody | PK136

Mouse anti Mouse CD161 / NK1.1

Product Type
Monoclonal Antibody
Clone
PK136
Isotype
IgG2a
Specificity
CD161 / NK1.1

Product Code Applications Pack Size List Price Your Price Qty
MCA1266
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SDS Safety Datasheet SDS
CT F IP 0.25 mg loader
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loader
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Mouse anti Mouse CD161 / NK1.1 antibody, clone PK136 recognizes the mouse NK1.1 cell surface antigen, a cell surface glycoprotein encoded by members of the NKR-P1 gene family. The NK1.1 surface antigen is also known as CD161b/CD161c and Ly-55.

In the mouse the NKR-P1 family has three members, NKR-P1A, -B and -C, whilst in the human only one member has been identified. The human protein has received the designation CD161, and the mouse proteins have been referred to as CD161a, -b, -c etc.

Although previously thought to recognize only CD161c, recent data has shown that the PK136 antibody may also react with CD161b. CD161c expression itself is strain specific in mice, but recognition of CD161b by PK136 appears to be even more complex, as only some CD161b positive strains are labelled by the antibody. Engagement of CD161c has been reported to have activating function in NK cells, whilst engagement of CD161b is inhibitory.

Mouse anti Mouse NK1.1 Antigen antibody, clone PK136 is useful for the identification of NK cells in selected strains of mice (positive on C57BL, FVB/N and NZB, but negative on AKR and BALB/c) and is also expressed by rare subsets of T cells and monocytes. Mouse anti Mouse NK1.1 antibody, clone PK136 has also been used for in vivo depletion of NK cells (Wang et al. 2022) and in vitro activation of NK cells (Kung and Miller 1995).

Target Species
Mouse
Species Cross-Reactivity
Target SpeciesCross Reactivity
Rat
Human
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% sodium azide (NaN3)
Carrier Free
Yes
Immunogen
Spleen and bone marrow cells from CE mice.
Approx. Protein Concentrations
IgG concentration 1 mg/ml
Fusion Partners
Spleen cells from immunised (C3H x BALB/c) FI Hybrid were fused with cells of the Sp2/0 - Ag14 myeloma cell line.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Cytotoxic Assays 1ug/ml 10ug/ml
Flow Cytometry 5ug/ml 10ug/ml
Immunoprecipitation
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10μl of the suggested working dilution to label 1 x 106 cells in 100μl

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Source Reference

  1. Koo, G.C. & Peppard, J.R. (1984) Establishment of monoclonal anti-Nk-1.1 antibody.
    Hybridoma. 3 (3): 301-3.

References for CD161 / NK1.1 antibody

  1. Kung, S.K. & Miller RG (1995) The NK1.1 antigen in NK-mediated F1 antiparent killing in vitro.
    J Immunol. 154 (4): 1624-33.
  2. Wang, M. et al. (1998) Natural killer cell depletion fails to influence initial CD4 T cell commitment in vivo in exogenous antigen-stimulated cytokine and antibody responses.
    J Immunol. 160 (3): 1098-105.
  3. Koo, G.C. et al. (1986) The NK-1.1(-) mouse: a model to study differentiation of murine NK cells.
    J Immunol. 137 (12): 3742-7.
  4. Kung, S.K. et al. (1999) The NKR-P1B gene product is an inhibitory receptor on SJL/J NK cells.
    J Immunol. 162 (10): 5876-87.
  5. Carlyle, J.R. et al. (1999) Mouse NKR-P1B, a novel NK1.1 antigen with inhibitory function.
    J Immunol. 162 (10): 5917-23.
  6. Carnemolla, B. et al. (2002) Enhancement of the antitumor properties of interleukin-2 by its targeted delivery to the tumor blood vessel extracellular matrix.
    Blood. 99: 1659-65.
  7. Carpentier, A.F. et al. (1999) Oligodeoxynucleotides containing CpG motifs can induce rejection of a neuroblastoma in mice.
    Cancer Res. 59: 5429-32.
  8. Sakai, T. et al. (2010) Inflammatory disease and cancer with a decrease in Kupffer cell numbers in Nucling-knockout mice.
    Int J Cancer. 126: 1079-94.
  9. View The Latest Product References
  10. Svensson, L. et al. (2003) gammadelta T cells contribute to the systemic immunoglobulin E response and local B-cell reactivity in allergic eosinophilic airway inflammation.
    Immunology. 108 (1): 98-108.
  11. Hazlett, L.D. et al. (2007) NKT cells are critical to initiate an inflammatory response after Pseudomonas aeruginosa ocular infection in susceptible mice.
    J Immunol. 179 : 1138-46.
  12. Joseph-Pietras, D. et al. (2006) Anti-tumoural activity of peripheral blood mononuclear cells against melanoma cells: discrepant in-vitro and in-vivo effects.
    Melanoma Res. 16: 325-33.
  13. Gock, H. et al. (2014) Altered glycosylation in donor mice causes rejection of strain-matched skin and heart grafts.
    Am J Transplant. 14 (4): 797-805.
  14. Khallouf, H. et al. (2012) 5-Fluorouracil and interferon-α immunochemotherapy enhances immunogenicity of murine pancreatic cancer through upregulation of NKG2D ligands and MHC class I.
    J Immunother. 35 (3): 245-53.
  15. Ebbinghaus, C. et al. (2005) Engineered vascular-targeting antibody-interferon-gamma fusion protein for cancer therapy.
    Int J Cancer. 116 (2): 304-13.
  16. Ekstrand-Hammarström, B. et al. (2011) Inhalation of alkylating mustard causes long-term T cell-dependent inflammation in airways and growth of connective tissue.
    Toxicology. 280 (3): 88-97.
  17. Klezovich-Bénard M et al. (2012) Mechanisms of NK cell-macrophage Bacillus anthracis crosstalk: a balance between stimulation by spores and differential disruption by toxins.
    PLoS Pathog. 8 (1): e1002481.
  18. Halin, C. et al. (2002) Enhancement of the antitumor activity of interleukin-12 by targeted delivery to neovasculature.
    Nat Biotechnol. 20 (3): 264-9.
  19. Gustafsson, Å. et al. (2015) Differential cellular responses in healthy mice and in mice with established airway inflammation when exposed to hematite nanoparticles.
    Toxicol Appl Pharmacol. 288 (1): 1-11.
  20. Flavell, D.J. et al. (2019) The TLR3 Agonist Poly Inosinic:Cytidylic Acid Significantly Augments the Therapeutic Activity of an Anti-CD7 Immunotoxin for Human T-cell Leukaemia.
    Biomedicines. 7 (1) Feb 16 [Epub ahead of print].
  21. Miao, M. et al. (2021) Reevaluation of NOD/SCID Mice as NK Cell-Deficient Models.
    Biomed Res Int. 2021: 8851986.

Further Reading

  1. Arase, N. et al. (1997) Association with FcRgamma is essential for activation signal through NKR-P1 (CD161) in natural killer (NK) cells and NK1.1+ T cells.
    J Exp Med. 186 (12): 1957-63.

Flow Cytometry

RRID
AB_322347
UniProt
P27814
P27812
Entrez Gene
Klrb1b
Klrb1c
GO Terms
GO:0004872 receptor activity
GO:0005887 integral to plasma membrane
GO:0005529 sugar binding
GO:0009897 external side of plasma membrane
GO:0042803 protein homodimerization activity
GO:0045953 negative regulation of natural killer cell mediated cytotoxicity
GO:0045954 positive regulation of natural killer cell mediated cytotoxicity
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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