Vinculin antibody | V284
Mouse anti Human vinculin antibody, clone V284 (MCA465GA) used to illustrate vinculin expression on murine retinal cell line 661W by immunofluorescence.
Image caption:
Morphology of 661 W on the PET membrane for control and after 120 min of applied EF. Cells were labeled for (a) nucleus,(b) vinculin, (c) filamentous actin cytoskeleton, and (d) merged image. The direction of the arrow shows the direction of the cathode and anode positions. Scale bar 50μm.
From: Rio, M., Bola, S., Funk, R. H. W., and Gerlach, G.
Microfluidic measurement of cell motility in response to applied non-homogeneous DC electric fields.
J. Sens Sens Syst, 5, 237-43.
This is from an open access article distributed under the terms of the Creative Commons Attribution License.
Mouse anti Human vinculin antibody, clone V284 (MCA465GA) used to assess accumulation of vinculin in myocutaneous junctions of the human tongue by immunohistochemistry on formalin fixed, paraffin embedded stissue sections.
Image caption:
Frontal section through the edge-wise and lower region of the tongue, The myocutaneous junctions showed some accumulation of desmin (d) but no accumulation of either beta1 integrin (e) or vinculin (f).
From: May CA.
Aponeurosis linguae-Myocutaneous or myotendinous junctions of skeletal muscle fibres in the human tongue?
J Anat. 2022 Feb 8 [Epub ahead of print].
doi: 10.1111/joa.13637.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Human vinculin antibody, clone V284 (MCA465GA) used as a loading control for chaperone proteins in western blotting.
Image caption:
Chaperone protein expression levels in treated 661W cells. Whole cell lysates were prepared and subjected to western blotting with antibody against (A) HSP70 and calnexin. Vinculin served as loading control.
From: Bola, S.; Subramanian, P.; Calzia, D.; Dahl, A.; Panfoli, I.; Funk, R.H.W.; Roehlecke, C.
Analysis of Electric Field Stimulation in Blue Light Stressed 661W Cells.
Int J Mol Sci. 2023, 24, 3433.
doi: 10.3390/ijms24043433.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Human vinculin antibody, clone V284 (MCA465GA) used to evaluate vinculin expression in rat alveolar epithelial cell lysates by western blotting.
Image caption:
30% cyclic stretch upregulated YAP downstream target gene expression in AEC II. (B) Western blot analysis of Cyr61/CCN1 and CTGF/CCN2 protein in whole cell lysates compared to non-stretched controls. The bar graphs show relative levels of protein normalized to vinculin and GAPDH (as loading controls). con: control. st: stretch. n = 6.
From: Ran X, Müller S, Brunssen C, Huhle R, Scharffenberg M, Schnabel C, Koch T, Gama de Abreu M, Morawietz H, Ferreira JMC, Wittenstein J.
Modulation of the hippo-YAP pathway by cyclic stretch in rat type 2 alveolar epithelial cells-a proof-of-concept study.
Front Physiol. 2023 Oct 9;14:1253810.
doi: 10.3389/fphys.2023.1253810.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Human Vinculin
- Product Type
- Monoclonal Antibody
- Clone
- V284
- Isotype
- IgG1
- Specificity
- Vinculin
Antibody Quality and Performance Guarantee
| Mouse anti Human vinculin antibody, clone V284 recognizes human vinculin, also known as metavinculin. Vinculin is a highly conserved, cytoskeletal protein associated with cell-cell and cell-matrix
adhesion. Vinculin is an actin filament (F-actin)-binding protein involved in anchoring F-actin to the membrane. Mouse anti Human vinculin antibody, clone V284 detects vinculin as a ~130 kDa band in homogenates of platelets and other cells. It reacts with leucocytes, muscle cells, epithelial cells and fibroblasts. |
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Rat Rabbit Chicken Mouse - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Carrier Free
- Yes
- Immunogen
- Purified vinculin from human platelets.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells of immunized mice were fused with cells from the SP2/0 mouse myeloma cell line.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry 1 |  |
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| Immunofluorescence | |
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| Immunohistology - Frozen | |
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| Immunohistology - Paraffin 2 | |
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| Immunoprecipitation | |
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| Western Blotting | |
1/500 | 1/2000 |
- 1 Membrane permeabilization is required for this application. The use of Leucoperm (Product Code BUF09) is recommended for this purpose.
- 2This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul
- Histology Positive Control Tissue
- Human appendix
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Mouse IgG1 Negative Control | MCA928 | F | 100 Tests |
|
Log in | ||
| List Price | Your Price | ||||||
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| Description | Mouse IgG1 Negative Control | ||||||
Source Reference
-
Asijee, G.M. et al. (1990) Vinculin is a permanent component of the membrane skeleton and is incorporated into the (re)organising cytoskeleton upon platelet activation.
Eur J Biochem. 189 (1): 131-6.
References for Vinculin antibody
-
Porter, R.M. et al. (1993) Monoclonal antibodies to cytoskeletal proteins: an immunohistochemical investigation of human colon cancer.
J Pathol. 170 (4): 435-40. -
Kubler, M.D. & Watt, F.M. (1993) Changes in the distribution of actin-associated proteins during epidermal wound healing.
J Invest Dermatol. 100 (6): 785-9. -
DeFife, K.M. et al. (1999) Cytoskeletal and adhesive structural polarizations accompany IL-13-induced human macrophage fusion.
J Histochem Cytochem. 47: 65-74. -
Jester, J.V. et al. (1999) Transforming growth factor(beta)-mediated corneal myofibroblast differentiation requires actin and fibronectin assembly.
Invest Ophthalmol Vis Sci. 40: 1959-67. -
Dlugosz, J.A. et al. (2000) Stretch-induced mesangial cell ERK1/ERK2 activation is enhanced in high glucose by decreased dephosphorylation.
Am J Physiol Renal Physiol. 279: F688-97. -
Brown, H. et al. (2001) Blood-brain barrier function in cerebral malaria in Malawian children.
Am J Trop Med Hyg. 64 (3-4): 207-13. -
Frangogiannis, N.G. et al. (2002) Active interstitial remodeling: an important process in the hibernating human myocardium.
J Am Coll Cardiol. 39: 1468-74. -
Jester, J.V. et al. (2003) Myofibroblast differentiation of normal human keratocytes and hTERT, extended-life human corneal fibroblasts.
Invest Ophthalmol Vis Sci. 44: 1850-8.
View The Latest Product References
-
Hua, H. et al. (2003) Endothelin-1 activates mesangial cell ERK1/2 via EGF-receptor transactivation and caveolin-1 interaction.
Am J Physiol Renal Physiol. 284: F303-12. -
Frye, M. et al. (2003) Evidence that Myc activation depletes the epidermal stem cell compartment by modulating adhesive interactions with the local microenvironment.
Development. 130: 2793-808. -
Monsees, T.K. et al. (2005) Effects of different titanium alloys and nanosize surface patterning on adhesion, differentiation, and orientation of osteoblast-like cells.
Cells Tissues Organs. 180: 81-95. -
Rebhun, R.B. et al. (2006) Targeting receptor tyrosine kinase on lymphatic endothelial cells for the therapy of colon cancer lymph node metastasis.
Neoplasia. 8: 747-57. -
Zheng, P.P. et al. (2007) Hela /-CaD undergoes a DNA replication-associated switch in localization from the cytoplasm to the nuclei of endothelial cells/endothelial progenitor cells in human tumor vasculature.
Cancer Biol Ther. 6: 886-90. -
Zheng, P.P. et al. (2007) Hela l-CaD is implicated in the migration of endothelial cells/endothelial progenitor cells in human neoplasms.
Cell Adh Migr. 1: 84-91. -
Gray, M.J. et al. (2008) Therapeutic targeting of Id2 reduces growth of human colorectal carcinoma in the murine liver.
Oncogene. 27: 7192-200. -
Andrews, K.D. et al. (2008) Vascular prostheses: performance related to cell-shear responses.
J Surg Res. 149: 39-46. -
Rio,M. et al. (2016) Microfluidic measurement of cell motility in response to applied non-homogeneous DC electric fields
Journal of Sensors and Sensor Systems. 5 (2): 237-43. -
Ananthaseshan, S. et al. (2017) Locally Transplanted CD34+ Bone Marrow-Derived Cells Contribute to Vascular Healing After Vascular Injury.
Transplant Proc. 49 (6): 1467-76. -
May, C.A. (2022) Aponeurosis linguae-Myocutaneous or myotendinous junctions of skeletal muscle fibres in the human tongue?
J Anat. Feb 08 [Epub ahead of print]. -
Bola, S. et al. (2023) Analysis of Electric Field Stimulation in Blue Light Stressed 661W Cells
Int J Mol Sci. 24 (4): 3433. -
Ran, X. et al. (2023) Modulation of the hippo-YAP pathway by cyclic stretch in rat type 2 alveolar epithelial cells-a proof-of-concept study.
Front Physiol. 14: 1253810.
- UniProt
- P18206
- Entrez Gene
- VCL
- GO Terms
- GO:0002009 morphogenesis of an epithelium
- GO:0002166 beta-dystroglycan binding
- GO:0002576 platelet degranulation
- GO:0006936 muscle contraction
- GO:0003779 actin binding
- GO:0005576 extracellular region
- GO:0005829 cytosol
- GO:0005198 structural molecule activity
- GO:0005925 focal adhesion
- View More GO Terms
- GO:0006928 cellular component movement
- GO:0007160 cell-matrix adhesion
- GO:0008013 beta-catenin binding
- GO:0030032 lamellipodium assembly
- GO:0030168 platelet activation
- GO:0030336 negative regulation of cell migration
- GO:0034333 adherens junction assembly
- GO:0034394 protein localization at cell surface
- GO:0043034 costamere
- GO:0043297 apical junction assembly
- GO:0045294 alpha-catenin binding
- GO:0045296 cadherin binding
- GO:0090136 epithelial cell-cell adhesion
MCA465GA
151502
156722
161426
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