Vinculin antibody | V284

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Mouse anti Human Vinculin

Product Type
Monoclonal Antibody
Clone
V284
Isotype
IgG1
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA465GA C F* IF IP P* WB 0.1 mg
Mouse anti Human vinculin antibody, clone V284 recognizes human vinculin, also known as metavinculin. Vinculin is a highly conserved, cytoskeletal protein associated with cell-cell and cell-matrix adhesion. Vinculin is an actin filament (F-actin)-binding protein involved in anchoring F-actin to the membrane.

Mouse anti Human vinculin antibody, clone V284 detects vinculin as a ~130 kDa band in homogenates of platelets and other cells. It reacts with leucocytes, muscle cells, epithelial cells and fibroblasts.

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Rat
Rabbit
Chicken
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Carrier Free
Yes
Immunogen
Purified vinculin from human platelets
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells of immunised mice were fused with cells from the SP2/0 mouse myeloma line

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch

More Information

UniProt
P18206 Related reagents
Entrez Gene
VCL Related reagents
GO Terms
GO:0002009 morphogenesis of an epithelium
GO:0002166 beta-dystroglycan binding
GO:0002576 platelet degranulation
GO:0006936 muscle contraction
GO:0003779 actin binding
GO:0005576 extracellular region
GO:0005829 cytosol
GO:0005198 structural molecule activity
GO:0005925 focal adhesion
GO:0006928 cellular component movement
GO:0007160 cell-matrix adhesion
GO:0008013 beta-catenin binding
GO:0030032 lamellipodium assembly
GO:0030168 platelet activation
GO:0030336 negative regulation of cell migration
GO:0034333 adherens junction assembly
GO:0034394 protein localization at cell surface
GO:0043034 costamere
GO:0043297 apical junction assembly
GO:0045294 alpha-catenin binding
GO:0045296 cadherin binding
GO:0090136 epithelial cell-cell adhesion
Regulatory
For research purposes only

Applications of Vinculin antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1
Immunofluorescence
Immunohistology - Frozen
Immunohistology - Paraffin 2
Immunoprecipitation
Western Blotting 1/500 1/2000
  1. 1 Membrane permeabilisation is required for this application. Bio-Rad recommends the use of Leucoperm™ (Product Code BUF09) for this purpose.
  2. 2This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul
Histology Positive Control Tissue
Human appendix

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Human anti Mouse IgG1:HRP HCA036P 0.1 mg E
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A 0.5 mg E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed) STAR117D549GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed) STAR117D649GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA 0.1 mg F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F 0.5 mg F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P 0.5 mg E WB
Goat anti Mouse IgG (Fc):FITC STAR120F 1 mg C F
Goat anti Mouse IgG (Fc):HRP STAR120P 1 mg E WB
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A 1 ml F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B 1 mg C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 0.5 mg F
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 1 ml F
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 0.5 mg C E P
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A 1 mg C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P 1 mg E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA 0.1 mg F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B 1 mg F

Negative Isotype Controls Available

Description Product Code Pack Size Applications List Price Quantity
Mouse IgG1 Negative Control MCA928 100 Tests F

Application Based External Images

Immunofluorescence

Immunohistology - Frozen

Western Blotting

Product Specific References

Source Reference

  1. Asijee, G.M. et al. (1990) Vinculin is a permanent component of the membrane skeleton and is incorporated into the (re)organising cytoskeleton upon platelet activation.
    Eur J Biochem. 189 (1): 131-6.
  2. Brown, H. et al. (2001) Blood-brain barrier function in cerebral malaria in Malawian children.
    Am J Trop Med Hyg. 64 (3-4): 207-13.

References for Vinculin antibody

  1. Porter, R.M. et al. (1993) Monoclonal antibodies to cytoskeletal proteins: an immunohistochemical investigation of human colon cancer.
    J Pathol. 170 (4): 435-40.
  2. Zheng, P.P. et al. (2007) Hela l-CaD is implicated in the migration of endothelial cells/endothelial progenitor cells in human neoplasms.
    Cell Adh Migr. 1: 84-91.
  3. Frangogiannis, N.G. et al. (2002) Active interstitial remodeling: an important process in the hibernating human myocardium.
    J Am Coll Cardiol. 39: 1468-74.
  4. Andrews, K.D. et al. (2008) Vascular prostheses: performance related to cell-shear responses.
    J Surg Res. 149: 39-46.
  5. Dlugosz, J.A. et al. (2000) Stretch-induced mesangial cell ERK1/ERK2 activation is enhanced in high glucose by decreased dephosphorylation.
    Am J Physiol Renal Physiol. 279: F688-97.
  6. Frye, M. et al. (2003) Evidence that Myc activation depletes the epidermal stem cell compartment by modulating adhesive interactions with the local microenvironment.
    Development. 130: 2793-808.
  7. Gray, M.J. et al. (2008) Therapeutic targeting of Id2 reduces growth of human colorectal carcinoma in the murine liver.
    Oncogene. 27: 7192-200.
  8. Jester, J.V. et al. (1999) Transforming growth factor(beta)-mediated corneal myofibroblast differentiation requires actin and fibronectin assembly.
    Invest Ophthalmol Vis Sci. 40: 1959-67.
  9. Jester, J.V. et al. (2003) Myofibroblast differentiation of normal human keratocytes and hTERT, extended-life human corneal fibroblasts.
    Invest Ophthalmol Vis Sci. 44: 1850-8.
  10. DeFife, K.M. et al. (1999) Cytoskeletal and adhesive structural polarizations accompany IL-13-induced human macrophage fusion.
    J Histochem Cytochem. 47: 65-74.
  11. Hua, H. et al. (2003) Endothelin-1 activates mesangial cell ERK1/2 via EGF-receptor transactivation and caveolin-1 interaction.
    Am J Physiol Renal Physiol. 284: F303-12.
  12. Zheng, P.P. et al. (2007) Hela /-CaD undergoes a DNA replication-associated switch in localization from the cytoplasm to the nuclei of endothelial cells/endothelial progenitor cells in human tumor vasculature.
    Cancer Biol Ther. 6: 886-90.
  13. Monsees, T.K. et al. (2005) Effects of different titanium alloys and nanosize surface patterning on adhesion, differentiation, and orientation of osteoblast-like cells.
    Cells Tissues Organs. 180: 81-95.
  14. Rebhun, R.B. et al. (2006) Targeting receptor tyrosine kinase on lymphatic endothelial cells for the therapy of colon cancer lymph node metastasis.
    Neoplasia. 8: 747-57.
  15. Sluka, P. et al. (2006) FSH regulates the formation of adherens junctions and ectoplasmic specialisations between rat Sertoli cells in vitro and in vivo.
    J Endocrinol. 189: 381-95.
  16. Ananthaseshan, S. et al. (2017) Locally Transplanted CD34+ Bone Marrow-Derived Cells Contribute to Vascular Healing After Vascular Injury.
    Transplant Proc. 49 (6): 1467-76.
  17. Rio,M. et al. (2016) Microfluidic measurement of cell motility in response to applied non-homogeneous DC electric fields
    Journal of Sensors and Sensor Systems. 5 (2): 237-43.
  18. Kubler, M.D. & Watt, F.M. (1993) Changes in the distribution of actin-associated proteins during epidermal wound healing.
    J Invest Dermatol. 100 (6): 785-9.